Article
Biochemistry & Molecular Biology
Mi Shen, Zixin Chen, Mengru Ming, Zhenghui Cheng, Junjie Sun, Qingyun Liang, Tongxin Shang, Qi Zhang, Songlin Zhou, Yuhua Ji, Fei Ding
Summary: Lysine acetylation is a reversible modification that plays a role in modulating the biology of Schwann cells (SCs) and regeneration of the peripheral nervous system (PNS). This study characterized the acetylome of the mouse sciatic nerve (SN) and identified acetylated proteins mainly located in the myelin sheath, mitochondrial inner membrane, and cytoskeleton. The results provide valuable insights into the role and mechanism of protein acetylation in SC development and PNS regeneration.
JOURNAL OF NEUROCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Vahid Zolfagharzadeh, Jafar Ai, Hadi Soltani, Sajad Hassanzadeh, Mehdi Khanmohammadi
Summary: We developed injectable biomimetic microspheres for repairing sciatic tissue using a microfluidic device. The microspheres, loaded with insulin, were fabricated using laccase-mediated crosslinking. The physical properties of the microspheres, such as swelling rate and release rate, showed that they were suitable for tissue engineering and drug delivery systems. The microspheres also demonstrated cytocompatibility and promoted nerve regeneration in a rat model, resulting in improved functional characteristics of the sciatic tissue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biology
Sonia Taib, Noel Lamande, Sabrina Martin, Fanny Coulpier, Piotr Topilko, Isabelle Brunet
Summary: Peripheral nerves rely on blood vessel invasion to ensure their function, which occurs during both embryonic development and the post-natal period. Netrin-1 promotes nerve invasion by blood vessels via the endothelial receptor UNC5B during embryogenesis, while myelinated Schwann cells negatively regulate intra-nervous vascularization during the post-natal period.
Article
Neurosciences
Mingchao Li, Matthew C. Banton, Qing Min, David B. Parkinson, Xinpeng Dun
Summary: This study identified 55 transcription factors that are upregulated in the distal nerve following mouse sciatic nerve injury, through analysis of microarray and single-cell RNA sequencing data. Further analysis of the expression profile and function of these transcription factors in cells of the distal nerve stump, as well as validation of specific transcription factors expression patterns, were conducted to provide insights into the role of key transcription factors in peripheral nerve regeneration.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Review
Biotechnology & Applied Microbiology
Misganaw Asmamaw Mengstie
Summary: The review examines the use of viral vectors as a means of delivering CRISPR/Cas9 and discusses the benefits and limitations of each vector. Viral vectors, such as AdVs, AAVs, and LVs, are commonly used for in vivo CRISPR/Cas9 delivery due to their higher delivery effectiveness. Future thoughts on overcoming current restrictions and adapting the technology are also discussed.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Pharmacology & Pharmacy
Artem A. Sizikov, Petr I. Nikitin, Maxim P. Nikitin
Summary: Nanoparticle-based technologies are being rapidly developed in various fields including biomedicine and molecular science. Magnetic nanoparticles have unique abilities to respond to magnetic fields, making them attractive for in vivo applications such as magnetofection. However, data on in vivo magnetofection processes after systemic injection of magnetic nanostructures are often limited, poorly studied, and sometimes contradictory. Emphasis has been placed on analyzing the structure and coating of nanomagnetic vectors, as well as considering current challenges and future trends in method development.
Article
Biochemistry & Molecular Biology
Peng Shang, Nadezda A. Stepicheva, Haitao Liu, Olivia Chowdhury, Jonathan Franks, Ming Sun, Stacey Hose, Sayan Ghosh, Meysam Yazdankhah, Anastasia Strizhakova, Donna Beer Stolz, J. Samuel Zigler, Debasish Sinha
Summary: This study introduces a novel and efficient method for RPE explant culture, allowing for obtaining biologically relevant RPE cells in situ, which is beneficial for pharmacological and mechanistic studies. The method involves culturing RPE explants for downstream analysis, such as transduction with adenoviral constructs and chemical treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Xiao-Feng Zhao, Lucas D. Huffman, Hannah Hafner, Mitre Athaiya, Matthew C. Finneran, Ashley L. Kalinski, Rafi Kohen, Corey Flynn, Ryan Passino, Craig N. Johnson, David Kohrman, Riki Kawaguchi, Lynda J. S. Yang, Jeffery L. Twiss, Daniel H. Geschwind, Gabriel Corfas, Roman J. Giger
Summary: This study performed single-cell analysis to investigate the pathological changes following nerve injury in mice. It was found that macrophages rapidly accumulated at the site of nerve injury and underwent metabolic reprogramming. The blood-nerve barrier showed partial leakiness, resulting in proliferation of stromal cells and entry of serum proteins. Distinct immune compartments were identified in response to mechanical nerve injury and Wallerian degeneration. Delayed Wallerian degeneration affected the distribution of immune cells in both the injured nerve and the distal nerve.
Article
Virology
Miranda Sam, Mohammed Selman, Weilong Zhao, Jiwon Jung, Aarron Willingham, Uyen Phan, Gary C. Starling, Qinshan Gao
Summary: CVA21 is a naturally occurring RNA virus that shows potential in treating malignancies. Researchers have successfully engineered CVA21 to carry transgenes and demonstrated the possibility of using cells as carriers for delivering the virus, which opens up new possibilities for cell therapy strategies.
JOURNAL OF VIROLOGY
(2023)
Article
Neurosciences
Kai Zhang, Qingyao Wang, Yiyao Liang, Yu Yan, Haiqiong Wang, Xu Cao, Bing Shan, Yaoyang Zhang, Ang Li, Yanshan Fang
Summary: Nerve injury leads to changes in protein levels, with ADPGK significantly increased in the injured nerve. Upregulated ADPGK in macrophages post-injury promotes phagocytosis, suggesting a role in injury-induced inflammatory responses. This study provides insights into the molecular mechanisms underlying neural injury and inflammation.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2021)
Article
Neurosciences
Sedigheh Momenzadeh, Saeed Zamani, Fariba Dehghan, Carlos Barreiro, Mohammad-Saeid Jami
Summary: The hormone irisin is proposed as an effector of exercise on the nervous system, with potential to improve nerve function and maintain cell homeostasis. Studies showed that irisin administration can regulate nerve metabolism, enhance axonal transport, and induce neurotrophic support on the sciatic nerve, suggesting its therapeutic potential for peripheral neuropathies and nerve injuries.
EUROPEAN JOURNAL OF NEUROSCIENCE
(2021)
Article
Multidisciplinary Sciences
Ya-Chien Chan, Eike Kienle, Martin Oti, Antonella Di Liddo, Maria Mendez-Lago, Dominik F. Aschauer, Manuel Peter, Michaela Pagani, Cosmas Arnold, Andreas Vonderheit, Christian Schoen, Sebastian Kreuz, Alexander Stark, Simon Rumpel
Summary: Enhancers are important cis-regulatory elements that control cell-type specific gene expression patterns. In this study, the Self-Transcribing Active Regulatory Region Sequencing (STARR-seq) technique was transposed to the mouse brain using adeno-associated-viruses (AAV) to identify enhancer activities. The study successfully constructed a genomic map of enhancer activities in the mouse brain and provided insights into their expression patterns in different cell types.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Pedro Silva-Pinheiro, Pavel A. Nash, Lindsey Van Haute, Christian D. Mutti, Keira Turner, Michal Minczuk
Summary: Mutations in mitochondrial DNA can lead to clinically heterogeneous diseases. Here the authors demonstrate in vivo base editing of mouse mitochondrial DNA in a post-mitotic tissue by AAV delivery of DddA-derived cytosine base editor (DdCBE).
NATURE COMMUNICATIONS
(2022)
Article
Pharmacology & Pharmacy
Yasmin M. Ahmed, Raha Orfali, Doaa S. Hamad, Mostafa E. Rateb, Hanan O. Farouk
Summary: This study investigates the role of chitosan-ufasomes loaded with propranolol HC1 in enhancing sciatica in rats with cisplatin-induced sciatic nerve damage. The results show that PRO-CTS-UFAs significantly improved sciatic nerve structure and inhibited abnormal gene expression and oxidative stress. The study suggests that CTS-UFAs have potential applications in the treatment of sciatic nerve damage.
Article
Cell Biology
Ruslan Masgutov, Alina Zeinalova, Alexey Bogov, Galina Masgutova, Ilnur Salafutdinov, Ekaterina Garanina, Valeriia Syromiatnikova, Kamilla Idrisova, Adelya Mullakhmetova, Dina Andreeva, Liliya Mukhametova, Adilet Kadyrov, Igor Pankov, Albert Rizvanov
Summary: The plasmid construct pBud-coVEGF165-coFGF2 possesses no immunogenicity and biosafety when injected into the intact rat sciatic nerve, showing significant changes in pro-angiogenic stimuli and levels of cytokines in peripheral blood, suggesting systemic effects. Transient expression of growth factors and active transport of transcripts in the sciatic nerve indicate its potential for nerve regeneration when used as a gene therapy drug.
NEURAL REGENERATION RESEARCH
(2021)
Article
Neurosciences
Enric Domenech-Estevez, Hasna Baloui, Cendrine Repond, Katia Rosafio, Jean-Jacques Medard, Nicolas Tricaud, Luc Pellerin, Roman Chrast
JOURNAL OF NEUROSCIENCE
(2015)
Article
Cell Biology
Sergio Gonzalez, Ruani Fernando, Jade Berthelot, Claire Perrin-Tricaud, Emmanuelle Sarzi, Roman Chrast, Guy Lenaers, Nicolas Tricaud
Article
Neurosciences
Bruno Hivert, Delphine Pinatel, Marilyne Labasque, Nicolas Tricaud, Laurence Goutebroze, Catherine Faivre-Sarrailh
Article
Multidisciplinary Sciences
Ruani N. Fernando, Laurent Cotter, Claire Perrin-Tricaud, Jade Berthelot, Sylvain Bartolami, Jorge A. Pereira, Sergio Gonzalez, Ueli Suter, Nicolas Tricaud
NATURE COMMUNICATIONS
(2016)
Article
Geriatrics & Gerontology
Harun N. Noristani, Hassan Boukhaddaoui, Guillaume Saint-Martin, Pauline Auzer, Rahima Sidiboulenouar, Nicolas Lonjon, Eric Alibert, Nicolas Tricaud, Christophe Goze-Bac, Christophe Coillot, Florence E. Perrin
FRONTIERS IN AGING NEUROSCIENCE
(2017)
Review
Neurosciences
Nicolas Tricaud
FRONTIERS IN CELLULAR NEUROSCIENCE
(2018)
Article
Neurosciences
Ysander von Boxberg, Sylvia Soares, Sophie Fereol, Redouane Fodil, Sylvain Bartolami, Jacques Taxi, Nicolas Tricaud, Fatiha Nothias
Article
Biochemical Research Methods
Helene Hajjar, Hassan Boukhaddaoui, Amel Rizgui, Chamroeun Sar, Jade Berthelot, Claire Perrin-Tricaud, Herve Rigneault, Nicolas Tricaud
JOURNAL OF BIOPHOTONICS
(2018)
News Item
Medicine, Research & Experimental
Sylvain Bartolami, Nicolas Tricaud
M S-MEDECINE SCIENCES
(2012)
Review
Neurosciences
Hwan Tae Park, Jong Kuk Kim, Nicolas Tricaud
Article
Multidisciplinary Sciences
Nathalie Bernard-Marissal, Gerben van Hameren, Manisha Juneja, Christophe Pellegrino, Lauri Louhivuori, Luca Bartesaghi, Cylia Rochat, Omar El Mansour, Jean-Jacques Medard, Marie Croisier, Catherine Maclachlan, Olivier Poirot, Per Uhlen, Vincent Timmerman, Nicolas Tricaud, Bernard L. Schneider, Roman Chrast
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Multidisciplinary Sciences
Anca G. Radu, Sakina Torch, Florence Fauvelle, Karin Pernet-Gallay, Anthony Lucas, Renaud Blervaque, Veronique Delmas, Uwe Schlattner, Laurence Lafanechere, Pierre Hainaut, Nicolas Tricaud, Veronique Pingault, Nadege Bondurand, Nabeel Bardeesy, Lionel Larue, Chantal Thibert, Marc Bilaud
Article
Neurosciences
Gerben van Hameren, Graham Campbell, Marie Deck, Jade Berthelot, Benoit Gautier, Patrice Quintana, Roman Chrast, Nicolas Tricaud
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2019)
Review
Biochemistry & Molecular Biology
Nicolas Tricaud, Hwan Tae Park
CELLULAR AND MOLECULAR LIFE SCIENCES
(2017)