Article
Biochemical Research Methods
Daniel Spitzer, Maryam I. Khel, Tim Puetz, Jenny Zinke, Xiaoxiong Jia, Kathleen Sommer, Katharina Filipski, Frits Thorsen, Thomas M. Freiman, Stefan Guenther, Karl H. Plate, Patrick N. Harter, Stefan Liebner, Yvonne Reiss, Mariangela Di Tacchio, Sylvaine Guerit, Kavi Devraj
Summary: The neurovascular unit (NVU) is crucial for the proper functioning of the central nervous system and involves endothelial cells, pericytes, astrocytes, microglia, and neurons. Dysfunction of the blood-brain barrier (BBB), regulated by the NVU, is seen in various neurological diseases and is a major therapeutic target. In this study, the EPAM-ia method is introduced as a protocol for simultaneous isolation and analysis of NVU cells using flow cytometry. This method involves the differential processing and combination of NVU cell types followed by immunostaining and fluorescence-activated cell sorting. The EPAM-ia method has applications in transcriptomics, proteomics, and cell culture and can be employed for analyzing various cell types in neurological diseases associated with BBB dysfunction.
Review
Biochemistry & Molecular Biology
Lucio Diaz-Flores, Ricardo Gutierrez, Maria Pino Garcia, Miriam Gonzalez-Gomez, Lucio Diaz-Flores, Jose Luis Carrasco, Juan Francisco Madrid, Aixa Rodriguez Bello
Summary: Perivascular cells, including pericytes and CD34+ stromal cells/telocytes, play an important role in angiogenesis. The behavior and changes of these cells differ depending on the type of angiogenesis, involving various cell-to-cell interactions and signaling pathways. Perivascular cells and CD34+ stromal cells/telocytes have important functions in vessel development and tissue repair, while their involvement in intussusceptive angiogenesis is limited.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Agata N. Rumianek, Ben Davies, Keith M. Channon, David R. Greaves, Gareth S. D. Purvis
Summary: In this study, a novel transgenic mouse (hCD68-CreERT2) was successfully generated to target macrophages in vivo under continuous tamoxifen induction, without affecting the expression of other myeloid cells. Through mating with the tdTomato variant mouse, the efficiency and specificity of this transgenic mouse were validated.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Shinji Tanaka, Shuqiu Zheng, Yugesh Kharel, Russell G. Fritzemeier, Tao Huang, Daniel Foster, Nabin Poudel, Eibhlin Goggins, Yusuke Yamaoka, Kinga P. Rudnicka, Jonathan E. Lipsey, Hope Radel, Sophia M. Ryuh, Tsuyoshi Inoue, Junlan Yao, Diane L. Rosin, Susan R. Schwab, Webster L. Santos, Kevin R. Lynch, Mark D. Okusa
Summary: This study reveals the important role of S1P signaling in the progression of kidney fibrosis. S1P is mainly produced by sphingosine kinase 2 in kidney perivascular cells and exported via Spns2. S1P binds to S1P1 expressed in perivascular cells, enhancing the production of proinflammatory cytokines/chemokines upon injury, leading to immune cell infiltration and fibrosis. Inhibition of Spns2 can alleviate kidney fibrosis.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Immunology
Xu Liu, Feiping Xia, Xiao Wu, Ying Tang, Lu Wang, Qin Sun, Ming Xue, Wei Chang, Ling Liu, Fengmei Guo, Yi Yang, Haibo Qiu
Summary: The study presents a method to isolate primary mouse PMECs and their EVs, and successfully establishes an iMPMECs cell line that can provide sufficient quantities of EVs for further research. Identification of PMECs and their EVs can be achieved through morphological, phenotypic, and functional characteristics, laying the foundation for future studies.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Guo Yin, Tae Shin, Jiyeon Ock, Min-Ji Choi, Anita Limanjaya, Mi-Hye Kwon, Fang-Yuan Liu, Soon-Sun Hong, Ju-Hee Kang, Yong Gho, Jun-Kyu Suh, Ji-Kan Ryu
Summary: Pericyte-derived extracellular vesicle-mimetic nanovesicles (PC-NVs) play a crucial role in improving erectile function and peripheral nervous system (PNS) regeneration. The study found that PC-NVs stimulated endothelial cells, increased neuronal cell content, and promoted angiogenesis and nerve regeneration. PC-NVs also increased the expression of neurotrophic factors and cell survival signaling pathway activity, while reducing JNK signaling pathway activity. After local application of PC-NVs, motor and sensory functions significantly improved.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2022)
Article
Immunology
Haoxin Shi, Dong Li, Qing Shi, Zhenxia Han, Yuwei Tan, Xiaodong Mu, Miao Qin, Zengjun Li
Summary: The study established a 3D culture system for macrophages using collagen microcarriers, finding that macrophages grown in 3D culture had significantly different morphology, arrangement, and angiogenic capabilities compared to those in 2D culture. Whole-transcriptome sequencing revealed significant gene expression differences, including downregulation of important angiogenic factors, and decreased expression of genes related to key angiogenic pathways in 3D-cultured macrophages.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Karin Strohmeier, Martina Hofmann, Jaroslaw Jacak, Marie-Sophie Narzt, Marlene Wahlmueller, Mario Mairhofer, Barbara Schaedl, Wolfgang Holnthoner, Martin Barsch, Matthias Sandhofer, Susanne Wolbank, Eleni Priglinger
Summary: The study found altered gene expression on the cellular and tissue levels in patients with lipedema, and revealed morphological differences in the cellular junctions of endothelial cell cultures derived from healthy individuals and those with lipedema through machine learning analysis. Additionally, the secretome of SVF cells from lipedema patients was shown to induce endothelial barrier dysfunction in early-stage lipedema, as evidenced by increased leakiness of healthy human primary endothelial cells.
Article
Cell Biology
Jinhui Zhang, Wenya Fan, Lingling Neng, Bei Chen, Bin Zuo, Wei Lu
Summary: This study focused on investigating the role of long non-coding RNA Rian in regulating the growth and function of perivascular-resident macrophage-like melanocytes (PVM/Ms), which can modulate the permeability of the intrastrial fluid-blood barrier by promoting the expression of pigment epithelial-derived factor (PEDF) and aid in maintaining inner ear homeostasis.
Article
Multidisciplinary Sciences
Erika Wong, Nina Nguyen, Judith Hellman
Summary: Endothelial cells play crucial roles in vascular regulation and immunity, and dysfunction of these cells is associated with various medical conditions. This protocol outlines a method to isolate highly pure endothelial cells from mice, which can be used for in vitro studies on cytokine production, cellular interactions, coagulation pathways, and permeability. Additionally, this procedure allows for the exploration of specific gene functions in healthy and pathological responses, utilizing knockout and transgenic mouse lines.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Biochemistry & Molecular Biology
Isaura Martins, Dalila Neves-Silva, Mariana Ascensao-Ferreira, Ana Filipa Dias, Daniel Ribeiro, Ana Filipa Isidro, Raquel Quiteria, Diogo Paramos-de-Carvalho, Nuno L. Barbosa-Morais, Leonor Saude
Summary: Traumatic spinal cord injury leads to cellular events that result in tissue loss and neuroinflammation. The disruption of the blood-spinal cord barrier allows immune cells to infiltrate and creates a toxic microenvironment that hinders axonal regeneration. Understanding the vascular response to injury is crucial for improving spinal cord repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nanoscience & Nanotechnology
Fang Yu, Nivasini Selva Kumar, Satnam Singh, Walter Hunziker, Sum Huan Ng, Deepak Choudhury
Summary: A microfluidic immunopanning chip (MIC) has been developed for simultaneous isolation and purification of multiple types of primary brain microvascular cells, achieving higher efficiency and purity compared to conventional methods. Incorporation of microcarriers in the MIC increases cell capturing efficiency and serves as a substrate for subsequent cell culture. With low reagents consumption, multiplexing capabilities, and high isolation efficiency, the MIC shows great potential for rare cell isolation and broader applications in cell therapy.
MICROFLUIDICS AND NANOFLUIDICS
(2021)
Article
Cell Biology
Yiqiang Cao, Fei Wang, Yonggang Wang, Jiang Long
Summary: Agomelatine prevents brain ischemic/reperfusion injury by inhibiting macrophage infiltration and protecting brain endothelial cells in a stroke mouse model.
Article
Cell Biology
Susan M. Millard, Ostyn Heng, Khatora S. Opperman, Anuj Sehgal, Katharine M. Irvine, Simranpreet Kaur, Cheyenne J. Sandrock, Andy C. Wu, Graham W. Magor, Lena Batoon, Andrew C. Perkins, Jacqueline E. Noll, Andrew C. W. Zannettino, David P. Sester, Jean-Pierre Levesque, David A. Hume, Liza J. Raggatt, Kim M. Summers, Allison R. Pettit
Summary: The study reveals that surface marker staining of macrophages in mouse hematopoietic tissues comes from subcellular remnants associated with other cells, rather than intact macrophages. This phenomenon may undermine the accuracy of ex vivo molecular profiling of macrophages and lead to misattribution of macrophage-expressed genes to non-macrophage cells.
Article
Multidisciplinary Sciences
Kholoud K. Saleh, Haibi Xi, Corey Switzler, Emily Skuratovsky, Matthew A. Romero, Peggie Chen, Devin Gibbs, Lily Gane, Michael R. Hicks, Melissa J. Spencer, April D. Pyle
Summary: The study revealed that in patients with Duchenne muscular dystrophy, as disease severity increases, the frequencies of activated fibroblasts, fibro-adipogenic pro-genitor cells, and pro-inflammatory macrophages increase, with upregulation of extracellular matrix genes in endothelial cells, and a pronounced risk of clotting in severely dystrophic patients.
Article
Immunology
Arwa A. Faizo, Asma A. Bawazir, Majed N. N. Almashjary, Ahmed M. M. Hassan, Fadi S. S. Qashqari, Ahmed S. S. Barefah, Sherif A. A. El-Kafrawy, Thamir A. A. Alandijany, Esam I. I. Azhar
Summary: This study investigated the effect of iron deficiency on COVID-19-vaccine-induced humoral immunity. The comparison between iron-deficient individuals and healthy controls showed no significant difference in the production of neutralizing antibodies. The use of NP-based ELISA identified individuals unaware of exposure to SARS-CoV-2. In conclusion, there is currently a lack of evidence regarding the association between iron deficiency and the effectiveness of COVID-19 vaccines, and larger studies are needed for comprehensive conclusions.
Article
Environmental Sciences
Munawar Shah, Rasim Shahzad, Muhsan Ehsan, Bushra Ghaffar, Irfan Ullah, Punyawi Jamjareegulgarn, Ahmed M. Hassan
Summary: Global Navigation Satellite System (GNSS)-based ionospheric anomalies are now being used to study seismic activities. In this study, statistical and machine learning algorithms were applied to observe Total Electron Content (TEC) from GPS and GIMs data. TEC perturbation was observed at the Sukkur GNSS station near the Mirpur EQ epicenter 5-10 days before the main shock, but no anomalies were found in GIM-TEC data.
Article
Multidisciplinary Sciences
Sayed Sartaj Sohrab, Sherif Aly El-Kafrawy, Zeenat Mirza, Ahmed M. Hassan, Fatima Alsaqaf, Esam Ibraheem Azhar
Summary: A new coronavirus, MERS-CoV, was discovered in Jeddah, Saudi Arabia in 2012. It has been reported in 27 countries and can be transmitted to humans from camels. There is currently no vaccine or antiviral therapy available for this virus.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
S. Ganeshkumar, Amit Kumar, J. Maniraj, Y. Suresh Babu, Alok Kumar Ansu, Ashish Goyal, Iman Kareem Kadhim, Kuldeep K. Saxena, Chander Prakash, Reem Altuijri, M. Ijaz Khan, Ahmed M. Hassan
Summary: Nano-scale multi-layered composite coatings have the potential to improve cutting tool performance by providing wear protection, heat dissipation, reducing friction, and heat. This review explores the potential benefits of these coatings and discusses future research directions.
ARABIAN JOURNAL OF CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
K. V. Nagaraja, Umair Khan, J. K. Madhukesh, Ahmed M. Hassan, B. C. Prasannakumara, Nabil Ben Kahla, Samia Elattar, Jasgurpreet Singh Chohan
Summary: Access to dependable and environmentally friendly energy sources is crucial for a country's economic growth and long-term development. This study investigates the impact of solar radiation and chemical processes on modified nanofluids, revealing the heat transfer effects under different flow conditions and the relationship between reaction rate and concentration levels.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Nesma Mohamed Ahmed, Ahmed Mahrous Hassan, Mohamed Abdelwahab Kassem, Ahmed Mahmoud Hegazi, Youssef Fayez Elsaadawi
Summary: This study evaluated the dependability and performance of a photovoltaic water pumping system under real operating conditions by examining the effects of solar irradiance, panels' temperature, and components' efficiency. The results showed that radiance significantly affected the pump flow rate, and higher panel temperatures led to lower efficiency.
SCIENTIFIC REPORTS
(2023)
Article
Materials Science, Multidisciplinary
Qadeer Raza, Xiaodong Wang, Ahmed M. Hassan, Imran Siddique, Bagh Ali, Irfan Ali
Summary: This study focuses on the numerical modeling of coaxially swirling porous disk flow subject to the combined effects of mixed convection and chemical reactions. Numerical investigations were conducted to analyze the morphologies of aluminum oxide (Al2O3) and copper (Cu) nanoparticles under the influence of magnetohydrodynamics. A model considering the aggregate nanoparticle volume fraction based on single-phase simulation, along with the energy and mass transfer equations, was developed for the flow of hybrid nanofluids. The high-order, nonlinear, ordinary differential equations obtained from the governing system of nonlinear partial differential equations were solved numerically by the Runge-Kutta technique and the shooting method.
FRONTIERS IN MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
T. N. Tanuja, L. Kavitha, S. V. K. Varma, Umair Khan, El-Sayed M. Sherif, Ahmed M. Hassan, Ioan Pop, K. Sarada, Harjot Singh Gill
Summary: The current study investigates the flow of micropolar liquid in a saturated permeable medium sandwiched between clear and hybrid nanofluids in an inclined channel, considering radiation and dissipation effects. Copper and Aluminium oxide nanoparticles are included along with the base (water) fluid. The solution for velocity, micro rotational velocity, and temperature is obtained using regular perturbation technique. The study analyzes the heat transport rate, shear stress, and temperature for different physical parameters through graphs and tables, revealing the effects of material and magnetic parameters.
FRONTIERS IN MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
K. V. Nagaraja, K. Vinutha, J. K. Madhukesh, Umair Khan, Jasgurpreet Singh Chohan, El-Sayed M. Sherif, Ioannis E. Sarris, Ahmed M. Hassan, B. Shanker
Summary: This study investigates the effects of porous media and thermal radiation on the movement of Casson-based nano liquid over a curved surface. The governing equations are simplified using similarity variables and solved numerically. The findings show that increased curvature improves velocity but decreases temperature, while increased modified Hartmann number raises velocity but decreases porosity parameter. Thermal radiation raises temperature, while modified Hartmann numbers and Casson factor lower velocity but increase thermal profile. The research findings have potential applications in the design of heat exchangers and microscale engines.
FRONTIERS IN MATERIALS
(2023)
Article
Thermodynamics
Shuguang Li, Sana Akbar, Muhammad Sohail, Umar Nazir, Abha Singh, Mashael Alanazi, Ahmed M. Hassan
Summary: This study focuses on the application of magnetized hybrid nanofluid in engineering and advanced industrial processes, and investigates the effect of suspended nano-size particles on the fluid flow using buoyancy and viscous dissipation. A numerical model of three-dimensional hybrid nanofluid flow is developed, considering the heat transmission phenomenon and the influence of parameters such as magnetic parameter M, slip parameter K, rotation parameter λ, and buoyancy parameter λb. The velocity and temperature distributions are determined using the Runge-Kutta (4th order) method, and the influence of various parameters on the flow behavior is graphically presented. The results are validated numerically and will contribute significantly to the field of fluid mechanics.
CASE STUDIES IN THERMAL ENGINEERING
(2023)
Article
Thermodynamics
Wei Li, Umar Farooq, Hassan Waqas, Aiedh Mrisi Alharthi, Nahid Fatima, Ahmed M. Hassan, Taseer Muhammad, Ali Akgul
Summary: This study investigates the effect of thermal radiation on heat transfer in MHD flow using a hybrid nanofluid. The suitable similarity transformations and computation techniques are used to analyze the flow of the hybrid nanofluid passing through a surface with thermal radiation, Biot number, and melting phenomena. The results show that the present model of hybrid nanofluid has higher thermal energy storage efficiency compared to standard nanofluids.
CASE STUDIES IN THERMAL ENGINEERING
(2023)
Article
Thermodynamics
Yijie Li, Yuchi Leng, Mahnoor Sarfraz, Nevine M. Gunaime, Jawad Ahmed, Ahmed M. Hassan, Mohamed Abdelghany Elkotb
Summary: This study investigates the thermal characteristics of nanofluids over a rotating cylinder, finding that the presence of nanoparticles increases the thermal transport coefficient while multi-walled carbon nanotubes exhibit a higher heat transfer rate compared to single-walled carbon nanotubes.
CASE STUDIES IN THERMAL ENGINEERING
(2023)
Article
Multidisciplinary Sciences
Bawar Iftikhar, Sophia C. Alih, Mohammadreza Vafaei, Muhammad Faisal Javed, Muhammad Faisal Rehman, Sherzod Shukhratovich Abdullaev, Nissren Tamam, M. Ijaz Khan, Ahmed M. Hassan
Summary: Plastic sand paver blocks provide a sustainable alternative by reducing the need for cement and utilizing plastic waste. This study developed empirical models using gene expression programming and multi-expression programming to predict the compressive strength of these blocks. The research findings have the potential to promote the reuse of these blocks, contributing to environmental protection and economic advantage.
SCIENTIFIC REPORTS
(2023)
Article
Public, Environmental & Occupational Health
Sarah Ayman Al-numaani, Alaa Talat Al-Nemari, Sherif A. El-Kafrawy, Ahmed M. Hassan, Ahmed M. Tolah, Maimonah Alghanmi, Ayat Zawawi, Badr Essa Masri, Salwa I. Hindawi, Thamir A. Alandijany, Leena H. Bajrai, Abdullah Bukhari, Ahmad Bakur Mahmoud, Waleed S. Al Salem, Abdullah Algaissi, Remi N. Charrel, Esam I. Azhar, Anwar M. Hashem
Summary: High seroprevalence rates of Sandfly Fever Sicilian virus (SFSV) and Toscana virus (TOSV) were found in humans and livestock animals in the western region of Saudi Arabia, indicating their circulation in the area. The seropositivity was higher in non-Saudis and increased with age, especially for SFSV. Animal handlers had the highest seroprevalence rates, suggesting that contact with livestock animals could be a risk factor for infection.
Article
Chemistry, Multidisciplinary
Shabbir Ahmad, Hidemasa Takana, Kashif Ali, Yasmeen Akhtar, Ahmed M. Hassan, Adham E. Ragab
Summary: Tri-hybrid nanofluid (THNF) achieves higher heat transfer rate by combining three different nanoparticles. It has diverse properties and potential applications in various fields. The study explores the interaction of Lorentz force with THNF nanoparticles and reveals significant effects on the flow field.
NANOTECHNOLOGY REVIEWS
(2023)