Journal
NATURE PROTOCOLS
Volume 7, Issue 4, Pages 782-788Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2012.025
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Funding
- US National Institutes of Health National Center for Research Resources (NIH-NCRR) [P40 RR012546]
- Fundacion CNIC Carlos III
- Fundacion ProCNIC
- Comunidad de Madrid [FIBROTEAM S2011/BMD-2321]
- Spanish Ministry of Economy and Competitiveness [RYC-2006-001694, BFU-2008-0012BMC]
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The zebrafish heart has the capacity to regenerate after ventricular resection. Although this regeneration model has proved useful for the elucidation of certain regeneration mechanisms, it is based on the removal of heart tissue rather than on tissue damage. We recently characterized the cellular response and regenerative capacity of the zebrafish heart after cryoinjury (CI), an alternative procedure that more closely models the pathophysiological process undergone by the human heart after myocardial infarction (MI). After anesthesia, localized CI with a liquid nitrogen-cooled copper probe induced damage in 25% of the ventricle, in a procedure requiring <5 min. Here we present a detailed description of the technique, which provides a valuable system for the study of the mechanisms of heart regeneration and scar removal after MI in a versatile vertebrate model.
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