Article
Genetics & Heredity
Alfredo Rodriguez, Michael Epperly, Jessica Filiatrault, Martha Velazquez, Chunyu Yang, Kelsey McQueen, Larissa A. Sambel, Huy Nguyen, Divya Ramalingam Iyer, Ulises Juarez, Cecilia Ayala-Zambrano, David B. Martignetti, Sara Frias, Renee Fisher, Kalindi Parmar, Joel S. Greenberger, Alan D. D'Andrea
Summary: Overexpression of the TGF beta pathway impairs the proliferation of hematopoietic stem and progenitor cells in Fanconi anemia. Genetic disruption of Smad3, a transducer of the canonical TGF beta pathway, modifies the phenotype of Fancd2-deficient mouse models, leading to high levels of embryonic lethality. The activation of the non-canonical TGF beta-ERK pathway is critical for the survival of Fancd2-Smad3 double knockout mice, but it also results in enhanced genomic instability and ineffective hematopoiesis.
Article
Biochemical Research Methods
Boyang Zhang, Hanqin Li, Zhixing Hu, Houbo Jiang, Aimee B. Stablewski, Brandon J. Marzullo, Donald A. Yergeau, Jian Feng
Summary: The protocol involves injecting naive human pluripotent stem cells converted from the primed state into mouse blastocysts to generate mouse-human chimeric embryos. It allows for studying the development of hPSCs in mouse embryos and may facilitate the generation of human cells, tissues, and organs in animals.
Article
Chemistry, Physical
Veronika Hlavackova, Rojina Shrestha, Eva Hofmanova, Pavel Kejzlar, Jakub Riha, Deepa Bartak, Alena Sevcu, Katerina Cerna
Summary: In this study, a novel protocol for detecting both living and dead bacterial cells in bentonite was proposed. The protocol involved the addition of a dispersant, followed by two-step centrifugation to separate and concentrate the cells. The extracted cells were then examined using epifluorescence microscopy and molecular biology methods. The protocol showed a high extraction efficiency and successfully identified the microbial community composition in different bentonite samples.
APPLIED CLAY SCIENCE
(2023)
Article
Developmental Biology
Neil R. Horner, Shanmugasundaram Venkataraman, Chris Armit, Ramon Casero, James M. Brown, Michael D. Wong, Matthijs C. van Eede, R. Mark Henkelman, Sara Johnson, Lydia Teboul, Sara Wells, Steve D. Brown, Henrik Westerberg, Ann-Marie Mallon
Summary: The use of automated annotation technology in high-throughput embryo pipelines allows for rapid integration of large volumes of 3D imaging data. By utilizing wild-type control data, issues such as low mutant sample numbers and incomplete penetrance are addressed. Additionally, research has shown that developmental substages have an impact on automated phenotyping results.
Article
Biochemical Research Methods
Pallavi Chinnu Varghese, Debasree Dutta
Summary: This article presents a protocol for studying gene expression during murine pre-implantation embryonic development. It includes steps for isolating embryos, extracting RNA and synthesizing cDNA, and detecting protein localization through immunofluorescence assay with confocal microscopy.
Article
Multidisciplinary Sciences
Daiyu Ito, Sayaka Wakayama, Rina Emura, Masatoshi Ooga, Teruhiko Wakayama
Summary: The study showed that freeze-dried sperm can be preserved on thin plastic sheets, with comparable DNA integrity to glass ampoule sperm, and healthy offspring can be obtained. This method allows for easy transportation and storage, promoting collaboration between laboratories and conservation of genetic resources.
Article
Entomology
Arun Rajamohan, Jarrad R. Prasifka, Joseph P. Rinehart
Summary: Since 1990, cryopreservation of insect embryos has been used for fruit flies, but the development of similar protocols for other insects has been slower. This study successfully developed a cryopreservation protocol for the sunflower moth and introduced a new method to assess the water permeability of embryos. The modified method improved the embryos' permeability and resulted in higher embryo survival rates.
Article
Obstetrics & Gynecology
Marta Gianzo, Itziar Urizar-Arenaza, Iraia Munoa-Hoyos, Gorka Labaka, Zaloa Larreategui, Nicolas Garrido, Jon Irazusta, Nerea Subiran
Summary: The study suggest a potential relationship between human sperm aminopeptidase N (APN) levels and embryo development, with low APN levels being associated with higher quality and usable embryos. This may offer a new molecular biomarker for diagnosing male infertility and improving success rates of assisted reproduction techniques.
HUMAN REPRODUCTION
(2022)
Article
Plant Sciences
Ayame Imoto, Mizuki Yamada, Takumi Sakamoto, Airi Okuyama, Takashi Ishida, Shinichiro Sawa, Mitsuhiro Aida
Summary: The ClearSee tissue clearing method is reliable for studying embryo morphology and gene expression patterns in Arabidopsis thaliana, enabling high-quality 3D imaging with cellular resolution.
Article
Engineering, Electrical & Electronic
Nasiru Yahaya Ahmed, Hazlee Azil Illias, Hazlie Mokhlis
Summary: This study proposes a simplified guide for selecting the most suitable line arrester, considering placement configurations, double circuit outage prevention, and energy capabilities. A typical 275 kV double circuit transmission line in Malaysia was analyzed using the Electromagnetic Transient Program. Results show that installing four arresters, two per circuit, at the lower and upper phases respectively, is the most optimal and universal double outage mitigation technique.
ELECTRIC POWER SYSTEMS RESEARCH
(2023)
Review
Chemistry, Physical
Markssuel Marvila, Paulo de Matos, Erich Rodriguez, Sergio Neves Monteiro, Afonso R. G. de Azevedo
Summary: Construction and demolition activities generate large amounts of waste, requiring the extraction of vast quantities of natural aggregate. By substituting natural aggregate with recycled aggregate from waste, environmental problems can be reduced. While the use of recycled aggregate may slightly decrease concrete performance, this reduction is negligible when replacement levels are below 30%. Efforts should focus on improving the efficiency of recycled aggregate processing and standardization.
Article
Veterinary Sciences
Hiroyuki Imai, Soichiro Tsuda, Tokuko Iwamori, Kiyoshi Kano, Ken Takeshi Kusakabe, Etsuro Ono
Summary: This study aimed to establish a novel method for producing chimeric embryos via water-in-oil droplets, successfully evaluating the generation of chimeric embryos and highlighting the advantages in controlling stem cell numbers and operability.
EXPERIMENTAL ANIMALS
(2021)
Article
Genetics & Heredity
Abhishek Sampath Kumar, Luyi Tian, Adriano Bolondi, Amelia Aragones Hernandez, Robert Stickels, Helene Kretzmer, Evan Murray, Lars Wittler, Maria Walther, Gabriel Barakat, Leah Haut, Yechiel Elkabetz, Evan Z. Macosko, Leo Guignard, Fei Chen, Alexander Meissner
Summary: The use of single-cell technologies has provided improved understanding of the dynamic gene expression during embryonic development. This study utilized Slide-seq technology to create spatial transcriptomic maps of mouse embryos at different stages, and developed a new visualization tool, sc3D, for quantitative investigation of gene expression patterns. Several previously unannotated genes with distinct spatial patterns were identified along the main embryonic axes, and the conflicting transcriptional identities of "ectopic" neural tubes in mutant embryos were characterized. Overall, this study presents an experimental and computational framework for spatiotemporal investigation of embryonic structures and mutant phenotypes.
Article
Biochemistry & Molecular Biology
Weihong Fan, Misato Homma, Renliang Xu, Hiroki Kunii, Hanako Bai, Manabu Kawahara, Toshikazu Kawaguchi, Masashi Takahashi
Summary: The zona pellucida (ZP) is crucial for fertilization and embryonic development. Removing the ZP (ZPR) is necessary for various reproductive techniques, but traditional methods can have negative effects on development. This study shows that a two-step ZPR method combined with a customized well-of-well system can improve blastocyst rate and reduce toxic effects on zona free (ZF) embryo development.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Neurosciences
Noemi G. Gyori, Christopher A. Clark, Daniel C. Alexander, Enrico Kaden
Summary: Diffusion MRI is a valuable tool for probing tissue microstructure in the brain. While model-based techniques for white matter characterisation are mature, modelling grey matter is more challenging. Advances in measurement technology and modelling efforts offer the prospect of a clinically viable technique for revealing salient features of grey matter microstructure.
