One solution for cloning and mutagenesis: In-Fusion® HD Cloning Plus

We describe in this edition a single, convenient system for both cloning and site-directed mutagenesis including deletions, base substitutions and base insertions. In-Fusion HD* Cloning Plus is sequence independent, seamless, directional and flexible enough to use with any vector, and it consistently allows over 95% cloning efficiency. Mutagenesis can be carried out by combining the strength of the In-Fusion HD enzyme with inverse PCR.