Journal
NATURE METHODS
Volume 6, Issue 11, Pages 813-U45Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.1389
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Funding
- Canadian Institutes of Health Research [MOP-152556]
- Canada Research Chairs Program
- Faculte des etudes superieurs de l'Universite de Montreal
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Clonal selection strategies are central tools in molecular biology. We developed a general strategy to dissect protein functions through positive and negative clonal selection for protein-protein interactions, based on a protein-fragment complementation assay using Saccharomyces cerevisiae cytosine deaminase as a reporter. We applied this method to mutational or chemical disruption of protein-protein interactions in yeast and to dissection of the functions of an allosterically activated transcription factor, Swi6.
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