Article
Biotechnology & Applied Microbiology
Leonela Z. Carabajal Paladino, Ray Wilson, Priscilla Y. L. Tng, Vishaal Dhokiya, Elizabeth Keen, Piotr Cuber, Will Larner, Sara Rooney, Melanie Nicholls, Anastasia Uglow, Luke Williams, Michelle A. E. Anderson, Sanjay Basu, Philip T. Leftwich, Luke Alphey
Summary: This study presents two improved methods for genetic manipulation in Ae. aegypti, including the use of transgenic lines expressing Cre recombinase for gene recombination, and a plasmid-based method for site-specific integration of genetic elements using PhiC31. These methods expand the toolbox for synthetic biology in Ae. aegypti and can be easily transferred to other mosquito and insect species.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Oncology
Lei Wang, Jiang Long, Huan Chen, Shaoyang Sun, Kunpeng Lv, Qiang Li, Xu Wang
Summary: The study demonstrates a novel approach to therapeutically target specific focuses by inhibiting aberrant Wnt activity without interfering with normal tissues using synthetic cell-based therapy. Successful proof of principle in an in vivo model suggests the potential of combining synthetic cells with antigen receptors to enhance treatment accuracy.
INTERNATIONAL JOURNAL OF CANCER
(2021)
Article
Zoology
Cong Li, Rui Bi, Lin Wang, Yu-Hua Ma, Yong-Gang Yao, Ping Zheng
Summary: In this study, the in vivo spermatogenesis potential of tree shrew SSCs cultured across different passages was examined. It was found that long-term expansion (>50 passages) led to the loss of spermatogenesis ability in SSCs. RNA sequencing analysis revealed significant gene expression changes after sub-culturing primary SPG lines for more than 40 passages, and experimental validation confirmed DNA damage accumulation and mitochondrial dysfunction in high-passage cells. Supplementation with NAD(+) precursor NR showed beneficial effects in reducing DNA damage accumulation and mitochondrial dysfunction. This research provides a comprehensive analysis of the genetic and physiological attributes critical for the sustained expansion of undifferentiated SSCs in tree shrews and proposes an effective strategy for extended in vitro maintenance.
ZOOLOGICAL RESEARCH
(2023)
Review
Cell Biology
Nesma E. Abdelaal, Bereket Molla Tanga, Mai Abdelgawad, Sahar Allam, Mostafa Fathi, Islam M. Saadeldin, Seonggyu Bang, Jongki Cho
Summary: Male infertility is a major health issue affecting a significant percentage of couples worldwide. The basis of spermatogenesis lies in spermatogonial stem cells, which hold promise for the regeneration of impaired spermatogenesis. However, challenges such as cryopreservation efficiency, malignant cell contamination, and socio-cultural attitudes hinder the wider application of SSCs as alternatives for the treatment of male infertility.
Article
Biochemistry & Molecular Biology
Weixiang Song, Danchen Zhang, Jiaqi Mi, Wenfei Du, Yang Yang, Rong Chen, Cong Tian, Xiaodong Zhao, Kang Zou
Summary: E-cadherin and Cadherin-22 synergistically maintain the undifferentiation of SPCs through the pivotal intermediate molecule beta-catenin. LEF1, TCF3, STAT3, and HDAC4 were identified as co-regulatory factors of beta-catenin in regulating SPC fate.
CELL AND BIOSCIENCE
(2022)
Article
Biology
Zachary C. Stevenson, Megan J. Moerdyk-Schauwecker, Stephen A. Banse, Dhaval S. Patel, Hang Lu, Patrick C. Phillips
Summary: High-throughput transgenesis using synthetic DNA libraries is a powerful method for exploring genetic function. However, the need for library transgenesis has restricted its application to single-cell models. In this study, we present TARDIS, a method that overcomes these limitations and enables large-scale transgenesis in multicellular systems. We demonstrate its utility in Caenorhabditis elegans and show that transformation yields can be increased up to approximately 1000-fold compared to current methods.
Article
Cell Biology
Seung-Jun Lee, Ka-Hwa Kim, Dong-Joon Lee, Pyunggang Kim, Jinah Park, Seong-Jin Kim, Han-Sung Jung
Summary: Spermatogonial stem cell (SSC) self-renewal is regulated by reciprocal interactions between Sertoli cells and SSCs in the testis. The study focused on the mechanism by which MAST4 in Sertoli cells transmits the signal and regulates SSCs, especially cell cycle regulation. The results demonstrate that MAST4 plays a pivotal role in maintaining the cell cycle of SSCs via the CDK2-PLZF interaction.
CELL PROLIFERATION
(2023)
Article
Veterinary Sciences
Shengnan Wang, Yanchao Duan, Bingbing Chen, Shuai Qiu, Tianzhuang Huang, Wei Si
Summary: Gene editing technology is considered an effective way to establish animal models of human diseases. Using spermatogonial stem cells and sperm as gene editing objects is simple to operate. However, there is no research on gene editing of spermatogonial stem cells to obtain genetically modified sperm, and it is unknown whether cryopreservation has adverse effects on genetically modified sperm. Furthermore, clear cell surface markers to identify primate spermatogonial stem cells are lacking. In this experiment, the enhanced green fluorescent protein lentivirus was injected into seminiferous tubules through the testis reticulum using ultrasound guidance, resulting in the successful production of transgenic sperm with similar freezing and recovery rates to wild animals.
VETERINARY SCIENCES
(2023)
Article
Cell Biology
Fangfang Dong, Min Chen, Min Chen, Lin Jiang, Zhiming Shen, Longfei Ma, Chunsheng Han, Xudong Guo, Fei Gao
Summary: PRMT5 is abundantly expressed in spermatogonial stem cells and its deletion leads to loss of SSCs and male infertility. Deficiency of Prmt5 in SSCs results in abnormal proliferation, cell cycle arrest, and increased apoptosis, along with alterations in gene expression and histone modifications.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Entomology
Xien Chen, Subba Reddy Palli
Summary: The study identified the molecular mechanism of deltamethrin resistance in fall armyworm by showing the key role of P450 gene SfCYP321A8, which could be applied in resistance and functional genomics research. Transgenic lines expressing P450 genes were developed and showed significantly increased tolerance to deltamethrin through bioassays, highlighting the potential for further research on insecticide resistance in lepidopteran pests.
JOURNAL OF PEST SCIENCE
(2022)
Article
Veterinary Sciences
Dong-Hyeok Kwon, Gyeong-Min Gim, Kyeong-Hyeon Eom, Ji-Hyun Lee, Goo Jang
Summary: This study investigated the applicability of three transposon systems in bovine somatic and embryo cells, and determined which system is suitable for each cell type.
BMC VETERINARY RESEARCH
(2022)
Article
Cell Biology
Naoya Yamaguchi, Ziyi Zhang, Teseo Schneider, Biran Wang, Daniele Panozzo, Holger Knaut
Summary: This study reports a new approach to measure cell migration stresses in zebrafish embryos and suggests that collectively migrating cells may be pushed forward by their rear cells. The findings have important implications for understanding animal embryonic development and tissue motion.
NATURE CELL BIOLOGY
(2022)
Article
Cell Biology
Aaron J. Huebner, Rebecca A. Gorelov, Ruslan Deviatiiarov, Samuel Demharter, Tobias Kull, Ryan M. Walsh, Marty S. Taylor, Simon Steiger, John T. Mullen, Peter V. Kharchenko, Konrad Hochedlinger
Summary: Huebner et al. developed a 2D culture system to study gastric isthmus stem cells and found that Sox2 plays a role in specifying enterochromaffin cells in the stomach. They showed that cell types in the corpus and antrum regions share similar transcriptional states, including the poorly characterized isthmus stem cells. The 2D culture system can be converted into 3D organoids and was used to demonstrate the importance of Sox2 in generating enterochromaffin cells.
NATURE CELL BIOLOGY
(2023)
Review
Endocrinology & Metabolism
Lei Diao, Paul J. Turek, Constance M. John, Fang Fang, Renee A. Reijo Pera
Summary: Spermatogonial stem cells (SSCs) are essential adult stem cells involved in spermatogenesis and male fertility. Understanding the regulation of SSCs is crucial for studying their function and the causes of male infertility, as well as developing new treatments through in vitro culture and differentiation of SSCs. This review highlights the recent research progress on SSC regulation and discusses the potential application of SSCs in fertility restoration.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Cell & Tissue Engineering
Takashi Shinohara, Takuya Yamamoto, Hiroko Morimoto, Yusuke Shiromoto, Mito Kanatsu-Shinohara
Summary: This study found that spermatogonial stem cells (SSCs) can induce immune tolerance by enhancing the expression of immunosuppressive molecule PD-L1, leading to the successful birth of allogeneic offspring. It was also found that activation of the MAPK14-BCL6B pathway can induce PD-L1 expression and promote self-renewal of SSCs.
Article
Biotechnology & Applied Microbiology
Nirmalya Ganguli, Nilanjana Ganguli, Sunandini Chandra, Mayank Choubey, Debi P. Sarkar, Subeer S. Majumdar
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Sandip Mukherjee, Snehasis Das, Dipanjan Chattopadhyay, Shuvasree Sarkar, Subhendu Kumar Chatterjee, Dhrubajyoti Talukdar, Sutapa Mukherjee, Subeer S. Majumdar, Satinath Mukhopadhyay, Mihir K. Chaudhuri, Samir Bhattacharya
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2018)
Article
Cell Biology
Mrittika Chattopadhyay, Sandip Mukherjee, Subhendu K. Chatterjee, Dipanjan Chattopadhyay, Snehasis Das, Subeer S. Majumdar, Satinath Mukhopadhyay, Sutapa Mukherjee, Samir Bhattarcharya
CELLULAR SIGNALLING
(2018)
Article
Genetics & Heredity
Kamal Mandal, Rajesh K. Sarkar, Souvik Sen Sharma, Ayushi Jain, Subeer S. Majumdar
Article
Biochemistry & Molecular Biology
Mansi Shukla, Nirmalya Ganguli, Souvik Sen Sharma, Subeer S. Majumdar
JOURNAL OF CELLULAR BIOCHEMISTRY
(2018)
Article
Cell Biology
Indrashis Bhattacharya, Sayon Basu, Bhola Shankar Pradhan, Hironmoy Sarkar, Perumal Nagarajan, Subeer S. Majumdar
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2019)
Article
Reproductive Biology
Souvik Sen Sharma, Amandeep Vats, Subeer Majumdar
REPRODUCTIVE BIOLOGY
(2019)
Review
Biochemistry & Molecular Biology
Indrashis Bhattacharya, Souvik Sen Sharma, Subeer S. Majumdar
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2019)
Article
Multidisciplinary Sciences
Bhola Shankar Pradhan, Indrashis Bhattacharya, Rajesh Sarkar, Subeer S. Majumdar
SCIENTIFIC REPORTS
(2019)
Article
Developmental Biology
Bhola Shankar Pradhan, Indrashis Bhattacharya, Rajesh Sarkar, Subeer S. Majumdar
MOLECULAR HUMAN REPRODUCTION
(2020)
Article
Obstetrics & Gynecology
Sujith Raj Salian, Riddhi Kirit Pandya, Sindhura Lakshmi Koulmane Laxminarayana, Hanumantappa Krishnamurthy, Aswathi Cheredath, Prathima Tholeti, Shubhashree Uppangala, Guruprasad Kalthur, Subeer Majumdar, Stefan Schlatt, Satish Kumar Adiga
Summary: This study investigated the influence of handling mouse ITT at different temperatures and time durations on cellular niche and quality. The results showed that storing ITT at ultraprofound-hypothermic-temperature is most suitable for organotypic culture, while long-term exposure at profound-hypothermic-temperature may compromise cell viability. These findings provide valuable insights for human prepubertal fertility restoration research.
REPRODUCTIVE SCIENCES
(2021)
Article
Andrology
Rajesh K. Sarkar, Souvik Sen Sharma, Kamal Mandal, Neerja Wadhwa, Neetu Kunj, Alka Gupta, Rahul Pal, Umesh Rai, Subeer S. Majumdar
Summary: The study identified a crucial role of transcription factor MEIS1 in Sertoli cell maturation and normal spermatogenic progression, with its inhibition leading to impaired spermatogenesis and reduced fertility.
Article
Biochemistry & Molecular Biology
Snehasis Das, Dipanjan Chattopadhyay, Subhendu K. Chatterjee, Samim Ali Mondal, Subeer S. Majumdar, Satinath Mukhopadhyay, Nirmalendu Saha, Ravichandiran Velayutham, Samir Bhattacharya, Sutapa Mukherjee
Summary: The study revealed that FetA-mediated activation of Ras through the Ras-MEK-ERK pathway plays a crucial role in regulating PPARγ inhibitory phosphorylation, affecting the crosstalk between adipocytes and myocytes; FetA triggers TNFα production in inflamed adipocytes, leading to the activation of Ras, and subsequently, impairs adiponectin levels and disrupts mitochondrial ATP production in skeletal muscle cells, causing imbalance in energy homeostasis and loss of insulin sensitivity.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Multidisciplinary Sciences
C. Macchi, A. Moregola, M. F. Greco, M. Svecla, F. Bonacina, S. Dhup, R. K. Dadhich, M. Audano, P. Sonveaux, C. Mauro, N. Mitro, M. Ruscica, G. D. Norata
Summary: This study investigated the impact of MCT1 deficiency on CD8(+) T cell function and found that MCT1 deficiency impaired CD8(+) T cell proliferation, shifted ATP production to mitochondrial oxidative phosphorylation, and affected the number of CD8(+) T cells infiltrating adipose tissue during obesity.
Article
Cell Biology
Sayon Basu, Satya Pal Arya, Abul Usmani, Bhola Shankar Pradhan, Rajesh Kumar Sarkar, Nirmalya Ganguli, Mansi Shukla, Kamal Mandal, Surendra Singh, Kanchan Sarda, Subeer S. Majumdar
CELL AND TISSUE RESEARCH
(2018)
