Article
Pharmacology & Pharmacy
Exequiel O. J. Porta, Jaime A. Isern, Karunakaran Kalesh, Patrick G. Steel
Summary: In this study, a new therapeutic target for the treatment of leishmaniasis was discovered using an activity-based protein profiling strategy to investigate serine proteases (SPs) in Leishmania parasites. Significant differences in the expression levels of active Leishmania serine hydrolases (SHs) were observed throughout the parasite's life cycle and between different species. Two targetable SPs were identified in Leishmania mexicana using quantitative proteomic mass spectrometry. The results of this study provide templates for the discovery of new drugs for the treatment of leishmaniasis.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Kaitlin Schaefer, Irene Lui, James R. Byrnes, Emily Kang, Jie Zhou, Amy M. Weeks, James A. Wells
Summary: This study presents a proteomic approach called cell surface N-terminomics to identify precise cleavage sites on the surface of living cells. By tagging extracellular neo-N-termini, this method allows for a comprehensive analysis of proteolyzed membrane proteins in various cell types. The research demonstrates that single oncogenes can induce extracellular proteolytic remodeling, which may be relevant in cancerous cell growth, invasion, and migration. Cell surface N-terminomics is a versatile platform that can be used for targeted immunotherapies.
ACS CENTRAL SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Kaitlin Schaefer, Irene Lui, James R. Byrnes, Emily Kang, Jie Zhou, Amy M. Weeks, James A. Wells
Summary: Proteolytic cleavage of cell surface proteins plays a crucial role in processes such as cell-cell interactions, receptor activation, and shedding of signaling proteins. This study introduces a proteomic approach called cell surface N-terminomics, which allows for the identification of precise cleavage sites on living cells. By utilizing a peptide ligase called stabiligase, extracellular neo-N-termini can be efficiently tagged for proteomic analysis. The study demonstrates the versatility of this approach by identifying and characterizing numerous extracellular neo-N-termini in different cell types, including primary immune cells. The findings also reveal the involvement of single oncogenes, KRAS(G12V) and HER2, in the proteolytic remodeling of proteins associated with cancerous cell growth, invasion, and migration. Cell surface N-terminomics provides a generalizable platform for targeting proteolyzed epitopes using immunotherapies.
ACS CENTRAL SCIENCE
(2022)
Article
Multidisciplinary Sciences
Ziya Isiksacan, Angelo D'Alessandro, Susan M. Wolf, David H. McKenna, Shannon N. Tessier, Erdem Kucukal, A. Aslihan Gokaltun, Nishaka William, Rebecca D. Sandlin, John Bischof, Narla Mohandas, Michael P. Busch, Caglar Elbuken, Umut A. Gurkan, Mehmet Toner, Jason P. Acker, Martin L. Yarmush, O. Berk Usta
Summary: Transfusion of red blood cells is a valuable and widespread treatment in modern medicine. Current practices in blood transfusion overlook the variability in stored red blood cell quality. It is necessary to rethink transfusion medicine workflows and utilize novel technologies for assessing red blood cell quality.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Medicine, General & Internal
Jan A. Graw, Victoria Buenger, Lorenz A. Materne, Alexander Krannich, Felix Balzer, Roland C. E. Francis, Axel Pruss, Claudia D. Spies, Wolfgang M. Kuebler, Steffen Weber-Carstens, Mario Menk, Oliver Hunsicker
Summary: This study investigated the effects of different storage durations of PRBCs on ARDS patients. The results showed that prolonged storage of PRBCs did not increase mortality in adults with ARDS, but transfusion of long-term stored PRBCs was associated with prolonged dependence on RRT in critically ill patients with ARDS.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Sonali R. Bhagwat, Komal Choudhary, Nirali Pandya, Sadhana Sharma, Sanjeeva Srivastava, Amit Kumar, Krishnan Hajela
Summary: MBL Associated Serine Protease-1 (MASP-1) is an enzyme in the lectin complement pathway with substrates beyond the complement system, influencing physiological processes. The study identified 35 putative substrates of MASP-1, including alpha 2-antiplasmin, alpha1-acid glycoprotein, antithrombin III, and siglec-6. The cleavage of these substrates by MASP-1 may have implications in thrombosis and thrombolysis.
MOLECULAR IMMUNOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Run Yao, Danyang Yan, Xiangjie Fu, Ying Deng, Xi Xie, Ning Li
Summary: This study investigates the association between plasma/red blood cells (RBCs) transfusion ratio and in-hospital mortality in patients with acute type A aortic dissection (AAAD). The findings suggest that a 1:1 plasma/RBCs transfusion ratio is associated with the lowest mortality in AAAD patients. Plasma transfusion is identified as an independent risk factor for in-hospital mortality. There is a non-linear relationship between plasma/RBCs transfusion ratio and mortality.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2023)
Review
Physiology
Gregory Barshtein, Ivana Pajic-Lijakovic, Alexander Gural
Summary: Red blood cells' deformability plays a crucial role in adapting to flow conditions, with high deformability facilitating flow through small blood vessels. Loss of deformability may lead to cell rigidity and dysfunction.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Genetics & Heredity
Takehito Sugasawa, Yasuharu Kanki, Ritsuko Komine, Koichi Watanabe, Kazuhiro Takekoshi
Summary: This study conducted an animal experiment to identify novel RNA markers for detecting autologous blood transfusion (ABT) doping. The results showed significant expression fluctuations in genes depending on the storage period, with a large number of genes showing decreased expression compared to the control group. Further bioinformatics analysis identified three significant genes with drastically decreased expression levels according to the storage period. These findings provide valuable insights for developing a testing method for ABT doping in the future.
Article
Biochemistry & Molecular Biology
Dania Fischer, Fabian Thies, Omar Awad, Camilla Brat, Patrick Meybohm, Patrick C. Baer, Markus M. Mueller, Anja Urbschat, Thorsten J. Maier, Kai Zacharowski, Jessica Roos
Summary: The relationship between allogeneic blood transfusions and increased rates of cancer recurrence, metastasis and death in colorectal cancer patients is still not fully understood. This study focused on the in vitro effects of microparticles derived from red blood cell units on colon carcinoma cells and found that these microparticles did not significantly affect cell characteristics or signaling pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Obstetrics & Gynecology
Nchafatso G. Obonyo, Lawrence Y. Lu, Nicole M. White, Declan P. Sela, Reema H. Rachakonda, Derek Teo, Matthew Tunbridge, Beatrice Sim, Louise E. See Hoe, Jonathon P. Fanning, John-Paul Tung, Matthew McKnoulty, Gianluigi Li Bassi, Jacky Y. Suen, John F. Fraser
Summary: This study investigated the association between transfusion of blood products close to the end of shelf-life and clinical outcomes in obstetric inpatients. The results showed that transfusing older red blood cells or platelets was not associated with increased morbidity or mortality.
INTERNATIONAL JOURNAL OF GYNECOLOGY & OBSTETRICS
(2023)
Article
Surgery
Francesco Amico, Jimmy T. Efird, Gabrielle D. Briggs, Natalie J. Lott, Kate L. King, Rena Hirani, Zsolt J. Balogh
Summary: The objective of this study was to test the association between blood donor demographics and post-injury multiple organ failure (MOF) in transfused trauma patients. The results showed that donor sex, but not age, was significantly associated with MOF. These findings may guide future risk mitigation strategies specific to blood donor demographics.
Article
Biochemical Research Methods
Andrea Fossati, Alicia L. Richards, Kuei-Ho Chen, Devan Jaganath, Adithya Cattamanchi, Joel D. Ernst, Danielle L. Swaney
Summary: By utilizing data-independent technologies and spectral libraries, researchers have successfully provided a comprehensive overview of the plasma proteome in individuals with and without TB. Through the use of nonconventional proteases and traditional trypsin, they identified a large number of peptides and proteins, achieving differential expression of more than 400 proteins in TB infection.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Davide Schiroli, Lucia Merolle, Eleonora Quartieri, Roberta Chicchi, Tommaso Fasano, Tiziana De Luca, Giuseppe Molinari, Stefano Pulcini, Thelma A. Pertinhez, Erminia Di Bartolomeo, Rino Biguzzi, Roberto Baricchi, Chiara Marraccini
Summary: This study compared two methods for preparing optimal P-RBCs products for beta-thalassemia major patients, finding that method 1 resulted in higher RBC count and Hb content, while method 2 produced P-RBCs with lower levels of K+, iron, and storage lesions markers. Further clinical studies are needed to determine the impact of these differences on transfusion-related complications and the quality of life of beta TM patients.
Article
Engineering, Biomedical
Xiangjian Liu, Yuying Hu, Yuxin Pan, Meirong Fang, Zhen Tong, Yilan Sun, Songwen Tan
Summary: The study found that medium molecular weight sodium hyaluronate (MSH) significantly increased the recovery rate of thawed red blood cells (RBCs) and maintained the functions and properties of RBCs. After direct washing of thawed RBCs, residual MSH could be removed, contributing to the increased recovery rate.
MATERIALS TODAY BIO
(2021)