Journal
NANOTECHNOLOGY
Volume 24, Issue 28, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/0957-4484/24/28/285501
Keywords
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Funding
- Converging Research Center Program [2012K001366]
- WCU program [R31-10032]
- NRF [2013-003416, 2013007874]
- Ministry of Education, Science and Technology (WCU project) [2013003890, 2012K001365, 20120006564, 2012-0001020, R32-2012-000-10213-0]
- National Research Foundation of Korea [R31-2012-000-10032-0, 2009-0080242, 2010-50232, 2012-0009597, R32-2012-000-10213-0, 2010-0020821, 2009-0079103, 2010-50233] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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We present receptor-modified carbon nanotube sensors for the highly selective and sensitive detection of acetylcholine (ACh), one kind of neurotransmitter. Here, we successfully expressed the M1 muscarinic acetylcholine receptor (M1 mAChR), a family of G protein-coupled receptors (GPCRs), in E. coli and coated single-walled carbon nanotube (swCNT)-field effect transistors (FETs) with lipid membrane including the receptor, enabling highly selective and sensitive ACh detection. Using this sensor, we could detect ACh at 100 pM concentration. Moreover, we showed that this sensor could selectively detect ACh among other neurotransmitters. This is the first demonstration of the real-time detection of ACh using specific binding between ACh and M1 mAChR, and it may lead to breakthroughs for various applications such as disease diagnosis and drug screening.
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