Review
Chemistry, Applied
Petr Tuma, Frantisek Opekar, Pavel Dlouhy
Summary: This paper provides a comprehensive survey of using capillary and microchip electrophoresis with contactless conductivity detection for the analysis of drinking water, beverages, and food items. It highlights the analysis of food on microchips and modern trends such as dual-opposite injection. CE/(CD)-D-4 is characterized by its universality, analysis speed, simple sample preparation, and minimal sample consumption.
Review
Chemistry, Analytical
Theo Lienard-Mayor, Myriam Taverna, Stephanie Descroix, Thanh Duc Mai
Summary: This study provides a comprehensive overview of different strategies for combining droplet-based sample handling and preparation with electrophoretic separation. It focuses on three principal interfacing strategies, including droplets in immiscible phases, digital microfluidics with electrowetting-on-dielectric principle, and inkjet droplet generation. Different instrumental developments, pros and cons of designs, and application demonstrations of droplet-interfaced electrokinetic strategies are discussed.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Hongyu Nie, Zhihui Li, Xiaokun Wang, Rongmeng Gu, Hongyan Yuan, Yong Guo, Dan Xiao
Summary: In this study, a multi-input capacitively coupled contactless conductivity detector (MICD)-D-4 was proposed with high sensitivity, and further improved by combining it with differential detection to form (DFMICD)-D-4. The new detector showed improved performance compared to traditional single-input and double-input detectors, with a higher response-to-baseline ratio and a low limit of detection (0.7 nM). It can be applied to the detection of low-conductivity solutions and total dissolved solids (TDS) analysis, demonstrating its versatility and superiority over standard conductivity meters.
Article
Chemistry, Analytical
Thushara N. Samarasinghe, Yong Zeng, Carey K. Johnson
Summary: The separation of a set of model proteins was tested on a microchip electrophoresis analytical platform, with the best separation and resolution achieved on a silica-nanoparticle colloidal array chip.
JOURNAL OF SEPARATION SCIENCE
(2021)
Review
Biochemical Research Methods
Abdallah M. Zeid, Islam M. Mostafa, Baohua Lou, Guobao Xu
Summary: Pathogenic bacteria and viruses are the main causes of infectious diseases worldwide. Early diagnosis plays a critical role in controlling their spread and treating infections. The innovation of smart sensing platforms, including nanosensors, is essential for isolating patients and providing suitable treatment strategies. Nanosensors offer high sensitivity, specificity, portability, and fast analysis, and they can be used for pathogen detection in biological matrices. The integration of nanosensors with artificial intelligence enables precise detection of pathogenic bacteria and viruses in biological samples.
Review
Chemistry, Analytical
Petr Tuma
Summary: This review article covers the historical development and current techniques of using capillary and chip electrophoresis with contactless conductivity detection for amino acid determination. It discusses theory of conductivity detection, optimization of background electrolyte, chiral separation, multidimensional separation, and the use of multidetection systems. Additionally, it highlights clinical applications and modern sample treatment techniques.
Review
Biochemical Research Methods
Zhao Meng, Junmin Bi, Qianqian Zhang, Hang Ren, Weidong Qin
Summary: The review discusses the applications of nanomaterials in capillary electrophoresis (CE) and microchip capillary electrophoresis (MCE) to enhance sensitivity of various detection techniques. It provides an overview of the types of nanomaterials used, detection mechanisms, analytical performance, and applications.
Review
Chemistry, Analytical
Petr Tuma
Summary: Contactless conductivity detection (C4D) is a universal detection technique that plays a significant role in the analysis of clinical samples. It is particularly sensitive to low molecular weight biogenic substances and can directly quantify them in their native form, making it widely applicable.
ANALYTICA CHIMICA ACTA
(2022)
Article
Food Science & Technology
Maria Castro-Puyana, Maria L. Marina
Summary: This review article critically examines the significant advances in using CE-MS-based approaches to assess various aspects of Food Science, and discusses the latest developments in hardware and software.
CURRENT OPINION IN FOOD SCIENCE
(2023)
Review
Biochemical Research Methods
Abdallah M. Zeid, Islam M. Mostafa, Baohua Lou, Guobao Xu
Summary: Pathogenic bacteria and viruses are the main causes of infectious diseases, and early diagnosis is crucial. The development of smart sensing platforms for point-of-care diagnosis is necessary for isolating patients and providing suitable treatment strategies. Nanosensors, such as nanopore sensors and nanostructure-integrated lab-on-a-chip sensors, offer portable, cost-effective, and fast analysis. Furthermore, non-invasive nanosensors can detect volatile organic compounds emitted from diseased organs. The integration of smart miniaturized nanosensors with artificial intelligence enables precise detection of pathogenic bacteria or viruses in biological samples.
Article
Chemistry, Analytical
Jin Chen, Yu Sun, Xiaogai Peng, Yi Ni, Fengchao Wang, Xiaoming Dou
Summary: The lab-built microchip capillary electrophoresis system and space domain internal standard method were used for rapid and reliable detection and quantification of S. aureus. The SDIS method showed higher precision and accuracy compared to traditional methods, allowing for quick identification and quantitation of the pathogen.
Article
Biochemical Research Methods
Saara Mikkonen, Leila Josefsson, Meeri E-L Makinen, Veronique Chotteau, Asa Emmer
Summary: CE and MCE methods were developed and applied for the analysis of amino acids in cell culture supernatant. The results showed that these methods were sensitive and the relative concentration changes agreed well with HPLC results. In addition, CE and MCE methods were faster and resulted in lower sample and reagent consumption.
BIOTECHNOLOGY JOURNAL
(2022)
Review
Biochemical Research Methods
Alberto Valdes, Gerardo Alvarez-Rivera, Barbara Socas-Rodriguez, Miguel Herrero, Alejandro Cifuentes
Summary: This paper presents a revision of the main applications of capillary electromigration methods in food analysis and Foodomics, including the analysis of different types of molecules in foods and monitoring of food interactions and food processing. New developments in microchips and applications of capillary electrophoresis in Foodomics are discussed, as well as new procedures to investigate food quality, safety, nutritional value, storage, and bioactivity.
Article
Chemistry, Analytical
Sille Stepanova, Vaclav Kasicka
Summary: This review provides an overview of recent advances and applications of capillary electrophoresis and microchip capillary electrophoresis methods in proteomics and peptidomics. It covers topics such as sample preparation, hyphenation with mass spectrometry, and multidimensional separations. New developments in both bottom-up and top-down approaches in proteomics are discussed, along with various applications in protein posttranslational modifications, biological and biochemical research, clinical peptidomics and proteomics, and food analysis.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Chemistry, Analytical
Yali Chen, Ling Xia, Xiaohua Xiao, Gongke Li
Summary: The study achieved rapid and effective quantitation of carnosine and nicotinamide in cosmetics by using cyclic olefin copolymer microchips with both dynamic and static coatings. The static coating improved the hydrophilicity of the surface and prevented nonspecific peptide adsorption, while the dynamic coating adjusted flow velocity and enhanced column efficiency. The method showed potential in complex sample analysis.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Chemistry, Analytical
Thomas Bouvarel, Nathalie Delaunay, Valerie Pichon
ANALYTICA CHIMICA ACTA
(2020)
Review
Chemistry, Analytical
Julien Camperi, Valerie Pichon, Nathalie Delaunay
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2020)
Article
Chemistry, Analytical
Stan Perchepied, Nicolas Eskenazi, Chiara Giangrande, Julien Camperi, Thierry Fournier, Joelle Vinh, Nathalie Delaunay, Valerie Pichon
Article
Biochemical Research Methods
Julien Camperi, Audrey Combes, Thierry Fournier, Valerie Pichon, Nathalie Delaunay
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Analytical
Fatima Barakat, Karen Gaudin, Brune Vialet, Katell Bathany, Sebastien Benizri, Philippe Barthelemy, Ludivine Ferey
Article
Biochemical Research Methods
Amira Al Matari, Audrey Combes, Julien Camperi, Thierry Fournier, Valerie Pichon, Nathalie Delaunay
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2020)
Review
Chemistry, Analytical
Thomas Bouvarel, Nathalie Delaunay, Valerie Pichon
Summary: This review discusses the latest developments in miniaturization of molecularly imprinted polymers to meet the societal demand for reliable, fast, affordable, and solvent/sample saving analyses. Different polymerization routes for producing miniaturized molecularly imprinted polymers in capillaries or chip channels are investigated, along with their potential applications in various separation techniques.
JOURNAL OF SEPARATION SCIENCE
(2021)
Review
Food Science & Technology
Nathalie Delaunay, Audrey Combes, Valerie Pichon
Article
Biochemical Research Methods
Amira Al Matari, Anastasia Goumenou, Audrey Combes, Thierry Fournier, Valerie Pichon, Nathalie Delaunay
Summary: The hCG protein, consisting of alpha and beta subunits with various glycoforms, was previously analyzed for glycoforms at the intact level. A modified method now allows for improved separation and detection of hCG alpha and beta glycoforms, with a higher number of glycoforms detected. The new method shows potential for rapid quality control of glycoprotein-based pharmaceutical preparations.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Thomas Bouvarel, Christophe Chendo, Nathalie Delaunay, Valerie Pichon
Summary: A simple, selective, and sensitive method for determining benzoylecgonine in complex biological samples was developed, utilizing a miniaturized solid phase extraction step with a monolithic molecularly imprinted polymer directly coupled to UV detection. The method achieved high selectivity and reproducibility, with limits of detection and quantification meeting regulatory standards for BZE detection in urine without the need for additional chromatographic separation. The photopolymerization technique allowed for the production of monoliths of varying lengths to adapt to different applications.
Review
Biochemistry & Molecular Biology
Anastasia Goumenou, Nathalie Delaunay, Valerie Pichon
Summary: Glycosylation is a significant post-translational modification of proteins that can affect their basic properties, but a full characterization of glycosylation remains challenging due to sample complexity. Lectins, a group of proteins with affinity for specific sugar moieties, are commonly used for selective enrichment in affinity chromatography and solid phase extraction methods.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Review
Environmental Sciences
Marine Boudias, Alkiviadis Gourgiotis, Gilles Montavon, Charlotte Cazala, Valerie Pichon, Nathalie Delaunay
Summary: The article discusses the performances of different analytical approaches, followed by an overview of applications.
JOURNAL OF ENVIRONMENTAL RADIOACTIVITY
(2022)
Article
Chemistry, Analytical
Pengchao Cao, Valerie Pichon, Catherine Dreanno, Kada Boukerma, Nathalie Delaunay
Summary: Several ion-imprinted polymers (IIPs) were synthesized for selective extraction of Cu(II) ions. The best IIP exhibited high specificity and good selectivity, with an adsorption capacity of 63 μg g(-1) and an enrichment factor of 24. The method was successfully applied to real samples and Brunauer-Emmett-Teller analysis showed the impact of post-treatment on the porous structure.
Article
Biochemical Research Methods
Amira Al Matari, Christophe Chendo, Thomas Bouvarel, Mael Arveiler, Michael Tatoulian, Olivier Venier, Nathalie Delaunay, Valerie Pichon
Summary: Monitoring synthesis reactions in real time is important for detecting intermediates, understanding reaction mechanisms, and identifying impurities. Spectroscopic methods may not be effective for complex mixtures, while mass spectrometry (MS) offers accuracy and sensitivity for detecting, quantifying, identifying, and characterizing reagents and species. This study developed a universal setup using two valves to hyphenate a microreactor with MS, which showed good repeatability and a linear response for analyzing molecules of different nature and hydrophilicity, even in non-MS-compatible solvents. The setup allowed for monitoring compounds synthesized at different concentrations and was successfully used in studying an amination reaction with high repeatability.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Environmental
Marine Boudias, Sofiane Korchi, Alkiviadis Gourgiotis, Audrey Combes, Charlotte Cazala, Valerie Pichon, Nathalie Delaunay
Summary: In this study, a selective and specific sorbent for radium extraction was developed using ion-imprinted polymer technology. The researchers identified the best synthesis conditions by testing various parameters and comparing the properties of different polymers. The resulting ion-imprinted polymer showed both selectivity and specificity in retaining radium ions while minimizing interference from other ions.
CHEMICAL ENGINEERING JOURNAL
(2022)