Journal
NANO LETTERS
Volume 18, Issue 9, Pages 5797-5804Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.8b02446
Keywords
Amyloidogenesis; IAPP; amyloid beta; zebrafish; carbon nanotubes; amyloid diseases
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Funding
- ARC [CE140100036]
- Recruitment Program of 1000plan Youth and Startup Funds from Tongji University (Lin)
- ARC Australian Laureate Fellowship
- Monash International Postgraduate Research Scholarship (MIPRS)
- Shanghai Science and Technology Commission Belt and Road initiative program [17230743000]
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Amyloid diseases are global epidemics with no cure available. Herein, we report a first demonstration of in vivo mitigation of amyloidogenesis using biomimetic nano technology. Specifically, the amyloid fragments (b(a)) of beta-lactoglobulin, a whey protein, were deposited onto the surfaces of carbon nanotubes (b(a)CNT), which subsequently sequestered human islet amyloid polypeptide (IAPP) through functional-pathogenic double-protein coronae. Conformational changes at the b(a)-IAPP interface were studied by Fourier transform infrared, circular dichroism, and X-ray scattering spectroscopies. b(a)CNT eliminated the toxic IAPP species from zebrafish embryos, as evidenced by the assays of embryonic development, cell morphology, hatching, and survival as well as suppression of oxidative stress. In addition to IAPP, b(a)CNT also displayed high potency against the toxicity of amyloid-beta, thereby demonstrating the broad applicability of this biomimetic nanotechnology and the use of an embryonic zebrafish model for the high-throughput screening of a range of amyloidogenesis and their inhibitors in vivo.
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