Article
Biotechnology & Applied Microbiology
Junwen Zhang, Jeffrey E. Plowman, Bin Tian, Stefan Clerens, Stephen L. W. On
Summary: The study utilized MALDI-TOF MS analysis to identify different yeast strains from organic wine production facilities in Waipara, New Zealand, demonstrating a high level of accuracy and reliability in species differentiation. Thirteen indigenous species belonging to eight genera were identified from Pinot Noir ferments, with taxonomic diversity generally decreasing as fermentation progressed; consistent proteomic differences were also observed among different fermentation systems.
Article
Microbiology
Claudia Colabella, Debora Casagrande Pierantoni, Laura Corte, Luca Roscini, Angela Conti, Matteo Bassetti, Carlo Tascini, Vincent Robert, Gianluigi Cardinali
Summary: By employing next generation sequencing technology, a study on the variability of ribosomal RNA in fungi revealed a significant amount of variation at both strain and species levels, with a higher concentration within the ITS region. These variants, including internal variability and SNPs between strains, can be utilized for in-depth strain typing and ecological studies.
Article
Microbiology
Junwen Zhang, Jeffrey E. Plowman, Bin Tian, Stefan Clerens, Stephen L. W. On
Summary: This study assessed the potential of MALDI-TOF profiling in predicting the utility of yeast strains in the beverage sector. The results showed that broth cultures yielded more peaks but with less clarity compared to agar cultures. Hierarchical clustering methods provided a clearer resolution of different species and a broad overview of strain utility, while MDS and UMAP analyses provided more nuanced insights. PCA-based displays were less informative.
Article
Biotechnology & Applied Microbiology
Samart Dorn-In, Sirkka Mang, Karin Schwaiger
Summary: This study investigated cold-tolerant Clostridium spp. strains isolated from various samples, identifying them into different groups based on genetic and biochemical characteristics. Spiked beef samples showed changes in odor after storage, while maintaining similar color and meat quality compared to the control group.
Article
Immunology
Ming-Chi Li, Hung-Jen Tang, Chi-Jung Wu, Shin-Wei Wang, Shu-Li Su, Wei-Lun Liu, Wen-Chien Ko, Yee-Chun Chen
Summary: This study aimed to compare the identification performance of two MALDI-TOF MS systems and an oligonucleotide array for uncommon Candida species isolates. The results showed that the oligonucleotide array and the two MALDI-TOF MS systems had high identification rates for these isolates. Elevated fluconazole minimal inhibitory concentrations were observed for some uncommon Candida species.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2022)
Article
Biology
Marek Kieliszek, Adam Wasko, Katarzyna Michalak, Anna M. Kot, Kamil Piwowarek, Stanislaw Winiarczyk
Summary: This study demonstrates the influence of selenium on protein biosynthesis in yeast cells. Proteomic analysis reveals higher levels of specific proteins in selenium-enriched yeast cells, including malate dehydrogenase, RNA helicase, and tRNA editing protein. These findings provide a model for future research on metabolic gene expression.
OPEN LIFE SCIENCES
(2022)
Article
Infectious Diseases
In Young Yoo, Hyang Jin Shim, Sun Ae Yun, On Kyun Kang, Yoo Na Chung, Tae Yeul Kim, Hyeyoung Lee, Jayoung Kim, Yeon-Joon Park, Hee Jae Huh, Nam Yong Lee
Summary: The MicroIDSys Elite system showed good performance in identifying mycobacteria directly from liquid cultures, with a correct identification rate of 85.6% for primary liquid cultures and 95.2% for liquid media subcultures. This indicates that the system is a useful routine diagnostic tool.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Microbiology
Helene Faury, Ronan Le Guen, Vanessa Demontant, Christophe Rodriguez, Berenice Souhail, Adrien Galy, Sarah Jolivet, Raphael Lepeule, Jean-Winoc Decousser, Vincent Fihman, Paul-Louis Woerther, Guilhem Royer
Summary: This study provides important new insight into the clinical features and clades associated with ampicillin-susceptible and ampicillin-resistant Enterococcus faecium isolates. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry with a custom-built database may enable the differentiation of different clades, facilitating the management of these infections.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemical Research Methods
Junwen Zhang, Jeffrey E. Plowman, Bin Tian, Stefan Clerens, Stephen L. W. On
Summary: The study found that culture media significantly impact the MALDI-TOF spectra of yeast strains, recommending YPD agar for taxonomic studies and YPD broth for intra-subspecific differentiation of S. cerevisiae. The data also showed a correlation between MALDI spectra and the potential industrial application of individual yeast strains.
JOURNAL OF MICROBIOLOGICAL METHODS
(2021)
Article
Microbiology
Helene Faury, Ronan Le Guen, Vanessa Demontant, Christophe Rodriguez, Berenice Souhail, Adrien Galy, Sarah Jolivet, Raphael Lepeule, Jean-Winoc Decousser, Vincent Fihman, Paul-Louis Woerther, Guilhem Royer
Summary: This study provides important insights into the clinical features and clades associated with ampicillin-susceptible and ampicillin-resistant Enterococcus faecium isolates. The findings suggest that ampicillin-resistant isolates are associated with a longer hospital stay, history of prior hospitalization, and the carriage of multidrug-resistant organisms. Furthermore, the use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry with a custom-built database may facilitate the management of these infections by distinguishing between clade B, which is typically susceptible to ampicillin, and clade A1/A2, which are typically resistant.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Eva Gato, Ignacio Pedro Constanso, Bruno Kotska Rodino-Janeiro, Paula Guijarro-Sanchez, Tyler Alioto, Manuel Jesus Arroyo, Gema Mendez, Luis Mancera, Marta Gut, Ivo Gut, Miguel Alvarez-Tejado, German Bou, Marina Oviano
Summary: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been used for direct detection of KPC-producing isolates by analyzing the 11,109 Da mass peak representing the P019 protein. The study found the P019 peak prevalence in bla(KPC) K. pneumoniae isolates to be 49.2%, while it was absent in other carbapenemase or noncarbapenemase producers. Variability of P019 presence was observed in different geographical locations within the EuSCAPE collection, indicating a need for reconsideration when applying this technique in a clinical setting.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Microbiology
Luna Girolamini, Maria Rosaria Pascale, Marta Mazzotta, Simona Spiteri, Federica Marino, Silvano Salaris, Antonella Grottola, Massimiliano Orsini, Sandra Cristino
Summary: Legionella surveillance is crucial for preventing infection risks and studying the ecology of isolates, but difficulties in isolation may lead to underestimation of human infection. The discovery of a new Legionella species highlights the importance of paying attention to characteristics of isolates less associated with the Legionella genus during culture techniques.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Microbiology
Daniele Maubon, Cecile Garnaud, Lala Soavina Ramarozatovo, Rapelanoro Rabenja Fahafahantsoa, Muriel Cornet, Tahinamandranto Rasamoelina
Summary: Chromoblastomycosis and sporotrichosis, as neglected tropical diseases, have laboratory diagnosis methods that require expertise and have limitations in sensitivity and specificity. However, the application of molecular methods such as MALDI-TOF MS, PCR, and sequencing can greatly improve the speed and accuracy of diagnosis, providing user-friendly point-of-care tests for basic healthcare settings and field consultations.
Article
Immunology
Guotao Ding, Guiying Li, Mengyu Liu, Peng Sun, Danqi Ren, Yan Zhao, Teng Gao, Guoxing Yang, Yanfei Fang, Weihao Li
Summary: In this study, bacterial contamination on used medical face masks was analyzed using the pour plate method, bio-Mass spectrometry technology, and 16SrRNA gene sequencing technology. The results showed that the bacterial counts were higher on the inside surface of the masks compared to the outside. The most common bacteria found were Neisseria and Corynebacterium. It is recommended to replace masks worn for more than four hours.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Monika Borkowska, Ewelina Celinska
Summary: Yeasts play an important role in food production, and reliable methods for yeast identification and differentiation are needed to monitor production processes and ensure the authenticity and safety of foods. In this study, a qPCR-HRM protocol based on three targeted regions was developed for rapid differentiation of yeast species isolated from food products. The simultaneous analysis of the three regions resulted in rapid and accurate differentiation of all the yeast strains at species-level resolution.
Article
Mycology
Hazal Kandemir, Karolina Dukik, Ferry Hagen, Macit Ilkit, Yvonne Graeser, G. Sybren de Hoog
Article
Dermatology
Peter Mayser, Pietro Nenoff, Dieter Reinel, Dietrich Abeck, Jochen Brasch, Georg Daeschlein, Isaak Effendy, Gabriele Ginter-Hanselmayer, Yvonne Graeser, Uta-Christina Hipler, Peter Hoeger, Annette Kolb-Maeurer, Hagen Ott, Martin Schaller, Miriam Zidane
JOURNAL DER DEUTSCHEN DERMATOLOGISCHEN GESELLSCHAFT
(2020)
Article
Dermatology
Andreas Ebert, Michel Monod, Karine Salamin, Anke Burmester, Silke Uhrlass, Cornelia Wiegand, Uta-Christina Hipler, Constanze Krueger, Daniela Koch, Franziska Wittig, Shyam B. Verma, Archana Singal, Sanjeev Gupta, Resham Vasani, Abir Saraswat, Rengarajan Madhu, Saumya Panda, Anupam Das, Mahendra M. Kura, Akshy Kumar, Shital Poojary, Sibylle Schirm, Yvonne Graeser, Uwe Paasch, Pietro Nenoff
Article
Microbiology
Max Berlin, Christiane Kupsch, Lea Ritter, Benjamin Stoelcker, Anton Heusinger, Yvonne Graeser
Article
Dermatology
Jochen Brasch, Vera Beck-Jendroschek, Karin Voss, Alexander Achenbach, Hans-Gerhard Berger, Melanie Harder, Yvonne Graeser, Thomas Schwarz
Summary: This study reports on three cases of Arthroderma (A.) crocatum isolated from human skin in Germany. The characteristics and epidemiology of this geophilic and likely apathogenic dermatophyte are described, emphasizing the importance of a combination of KOH mount, culture, and genetic analysis for clinically meaningful conclusions. The study suggests that the prevalence of A. crocatum may be currently underestimated.
Article
Biotechnology & Applied Microbiology
Susanne Nieland, Susann Barig, Julian Salzmann, Frauke Gehrau, Arief Izzairy Zamani, Annabell Richter, Julia Ibrahim, Yvonne Graeser, Chyan Leong Ng, Klaus-Peter Stahmann
Summary: By comparing different fungi, it was found that Aspergillus fumigatus AR04 had a faster hyphal growth rate at higher temperatures, and could even grow at 49 degrees Celsius. The fungus reached a steady state at a higher dilution rate of 0.7 h(-1) at 40 degrees Celsius compared to other fungi.
MICROBIAL BIOTECHNOLOGY
(2021)
Article
Microbiology
Xue Kong, Chao Tang, Ashutosh Singh, Sarah A. Ahmed, Abdullah M. S. Al-Hatmi, Anuradha Chowdhary, Pietro Nenoff, Yvonne Graser, Steven Hainsworth, Ping Zhan, Jacques F. Meis, Paul E. Verweij, Weida Liu, G. Sybren de Hoog
Summary: A decade-long outbreak of dermatophytosis due to a terbinafine-resistant novel species has spread to several countries, with investigations into the molecular background of the SQLE gene revealing high resistance levels in certain isolates. Antifungal susceptibility tests on isolates from different countries identified three species and highlighted the limited distribution of high terbinafine resistance.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Klaus-Peter Wojak, Gertrud F. Ungermann, Ichsan Ichsan, Emilia Gomez-Molero, Klaus Jung, Michael Weig, Friedemann Nauck, Dirk Ziebolz, Yvonne Graeser, Roland Nau, Uwe Gross, Bernd Alt-Epping, Oliver Bader
Summary: Elderly patients with multiple morbidities or medical treatments are more susceptible to fungal infections, particularly from azole-resistant yeasts like Candida glabrata. Factors such as age and denture-wearing influence the distribution of colonizing fungal species in the oral cavity, with non-albicans Candida species being more common in the elderly.
Article
Dermatology
Jochen Brasch, Yvonne Graeser, Vera Beck-Jendroscheck, Karen Voss, Kaspar Torz, Grit Walther, Thomas Schwarz
Summary: Indian strains of Trichophyton mentagrophytes with reduced susceptibility to itraconazole were found in Germany as early as 2011, earlier than previously assumed. This variant of the pathogen cannot be phenotypically distinguished from customary strains and requires genetic identification. The variant is anthropophilic and may lead to further dissemination.
JOURNAL DER DEUTSCHEN DERMATOLOGISCHEN GESELLSCHAFT
(2021)
Article
Dermatology
Jochen Brasch, Yvonne Graeser, Vera Beck-Jendroscheck, Karen Voss, Kaspar Torz, Grit Walther, Thomas Schwarz
Summary: Study reports on Indian strains of Trichophyton mentagrophytes with reduced susceptibility to itraconazole due to genetic mutations in Germany, raising concerns about potential wider spread of this variant and the need for rapid and accurate antifungal testing.
JOURNAL DER DEUTSCHEN DERMATOLOGISCHEN GESELLSCHAFT
(2021)
Article
Immunology
Sven-Niklas Burmann, Frank Oellig, Yvonne Graeser, Alena-Lioba Michalowitz, Alexandros Paschos, Alexander Kreuter
Summary: Tinea corporis is a common superficial fungal infection, whereas tinea of the anogenital region is rare and mostly found in tropical countries. A distinctive variant of pubogenital tinea (PT) characterized by deep tissue infiltration and systemic symptoms has been reported, with transmission via sexual contacts hypothesized. In this case report, PT caused by T. quinckeanum experienced a strong inflammatory reaction following successful treatment with itraconazole.
INTERNATIONAL JOURNAL OF STD & AIDS
(2022)
Review
Microbiology
Silke Uhrlass, Shyam B. Verma, Yvonne Graeser, Ali Rezaei-Matehkolaei, Maryam Hatami, Martin Schaller, Pietro Nenoff
Summary: T. indotineae is a newly identified dermatophyte species that has spread in an epidemic form on the Indian subcontinent. It causes inflammatory and itchy dermatophytosis and displays resistance to terbinafine, with itraconazole being the most effective treatment.
Letter
Dermatology
Jochen Brasch, Yvonne Graeser, Katharina Drerup, Karen Voss, Thomas Schwarz
JOURNAL DER DEUTSCHEN DERMATOLOGISCHEN GESELLSCHAFT
(2023)
Article
Dermatology
Pietro Nenoff, Dieter Reinel, Peter Mayser, Dietrich Abeck, Guntram Bezold, Philipp P. Bosshard, Jochen Brasch, Georg Daeschlein, Isaak Effendy, Gabriele Ginter-Hanselmayer, Yvonne Graeser, Gudrun Hamm, Ulrich Hengge, Uta-Christina Hipler, Peter Hoeger, Alexandra Kargl, Annette Kolb-Maeurer, Constanze Krueger, Bartosz Malisiewicz, Johannes Mayer, Hagen Ott, Uwe Paasch, Martin Schaller, Silke Uhrlass, Miriam Zidane
Summary: Onychomycosis is a fungal infection of the fingernails and toenails. The updated German S1 guideline aims to simplify the selection and implementation of appropriate diagnostics and treatment for this condition. It is based on current international guidelines and a literature review conducted by a multidisciplinary committee.
JOURNAL DER DEUTSCHEN DERMATOLOGISCHEN GESELLSCHAFT
(2023)
Review
Dermatology
Yvonne Graeser, Ditte Marie Lindhardt Saunte
ACTA DERMATO-VENEREOLOGICA
(2020)