Article
Biotechnology & Applied Microbiology
Jae Eun Choi, Jae-Sik Jeon, Jae Kyung Kim
Summary: The prevalence of candidiasis, a highly contagious disease with high morbidity and mortality, has significantly increased worldwide in the past two decades. This study investigated the positive rate and infection trend of Candida albicans, a major causative agent of candidiasis, based on age, specimen type, and sex using real-time polymerase chain reaction-based testing of samples collected for sexually transmitted disease diagnosis in Korea between 2018 and 2020. The findings showed that the positive rate of C. albicans was higher among younger women when swab specimens were collected, and decreased with age. The study contributes significantly to the literature due to the scarcity of epidemiological studies using molecular techniques to detect C. albicans in sexually transmitted disease test samples globally.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Immunology
Nichole D. Brandquist, Cierra Lampman, Elias J. Smith, Lizeth Basilio, Akram Almansob, Peter C. Iwen, Jill R. Blankenship
Summary: In this study, clinical strains of Candida albicans collected from patients with systemic infections were classified by MLST analysis. These clinical strains exhibited different filamentation patterns across various inducing conditions, indicating a broad phenotypic diversity among clinical strains. The results emphasize the importance of using diverse clinical strains in pathogenesis assays.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Nupur Garg, Upasana Sahu, Sudeshna Kar, Farhan J. Ahmad
Summary: This study introduces a fast, sensitive, and specific LAMP technique for early detection of leprosy, which showed complete concurrence with conventional techniques in blind clinical validation studies. Visual detection methods were also standardized for easy-to-read and rapid diagnosis, eliminating the lack of accuracy and sensitivity in skin smear tests and the need for expensive lab equipments and trained technicians.
SCIENTIFIC REPORTS
(2021)
Article
Medicine, General & Internal
Iori Oka, Hideo Shigeishi, Kouji Ohta
Summary: This study aimed to investigate the relationship between oral Candida Albicans infection/co-infection and periodontitis in middle-aged and older Japanese individuals. The results showed that some patients with Candida Albicans also had Porphyromonas gingivalis co-infection, which was significantly associated with deeper periodontal pockets and bleeding on probing.
MEDICINA-LITHUANIA
(2022)
Article
Food Science & Technology
Rosalia Rodriguez-Riveiro, Amaya Velasco, Carmen G. Sotelo
Summary: This study compares five DNA extraction methods based on quantity, quality, and simplicity to determine the most suitable method for species identification. Significant differences were found among the methods in terms of yield, efficiency, and purity, but not in terms of the species employed. The Wizard kit showed the highest yield, while other methods stood out for affordability (Chelex) and automation (Mpure).
Article
Virology
K. Shahar-Nissan, M. Oikawa Tepperberg, E. Mendelson, E. Bilavsky
Summary: This study assessed the clinical characteristics of children retrospectively diagnosed with congenital cytomegalovirus (cCMV) using dried blood-spot (DBS) polymerase chain reaction (PCR) testing and reported missed opportunities for early diagnosis and treatment. Universal newborn screening for cCMV could have prevented poor hearing and developmental outcomes in some cases.
JOURNAL OF CLINICAL VIROLOGY
(2022)
Article
Mycology
Qiuyu Pang, Weida Liu, Fan Cui, Siyue Kan, Xiaofang Li
Summary: This study investigated the genotypes and drug resistance of Candida albicans strains causing different conditions of vulvovaginal candidiasis (VVC). Results showed that C. albicans was the main pathogen of VVC, but non-albicans Candida (NAC) infection and mixed infection cases were increasing. Some C. albicans strains exhibited resistance to multiple drugs. The genotypes of RVVC and drug-resistant C. albicans strains were different from those of uncomplicated VVC. These findings have significant diagnostic and therapeutic implications for VVC.
Article
Nanoscience & Nanotechnology
Shun-ichi Matsuura, Tomoya Baba, Takuji Ikeda, Katsutoshi Yamamoto, Tatsuo Tsunoda, Aritomo Yamaguchi
Summary: The mesoporous silica-enhanced PCR technology allows highly precise and sensitive DNA amplification/detection from minimal amounts of nucleic acid, making it applicable for genetic testing involving small samples.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Georgiana Negru, Laure Kamus, Elena Bicu, Sergiu Shova, Boualem Sendid, Faustine Dubar, Alina Ghinet
Summary: The reaction between benzylidenemalononitriles and phenylhydrazines in refluxing ethanol did not lead to pyrazole derivatives, but instead produced hydrazones, whose structure was confirmed by X-ray analysis. The newly synthesized hydrazones showed promising antifungal activity against Candida spp., with five compounds demonstrating selective antifungal activity with MIC99 values ranging from 16 to 32 μg/mL.
Article
Infectious Diseases
Katherine Aguirre-Guataqui, Mateo Marquez-Torres, Hector Manuel Pineda-Castaneda, Yerly Vargas-Casanova, Andres Ceballos-Garzon, Zuly Jenny Rivera-Monroy, Javier Eduardo Garcia-Castaneda, Claudia Marcela Parra-Giraldo
Summary: Antimicrobial peptides are valuable resources for the discovery or design of potential antifungal candidates. Bovine lactoferricin and Buforin II are AMPs with great antimicrobial potential. Chimeras containing the minimal active motifs of these peptides were synthesized and evaluated for their antifungal activity against various strains of Candida. Some chimeras exhibited strong antifungal activity against specific strains.
Article
Public, Environmental & Occupational Health
S. Umamaheshwari, M. N. Sumana
Summary: This study retrospectively analyzed the distribution of Candida in various clinical samples in a tertiary care hospital in India. It found that urine samples were the major source of Candida infection, and non-albicans Candida (NAC) was the predominant species. The susceptibility test revealed that about 75.44% of isolates were susceptible to the tested drugs, while about 24.56% were resistant to one or more drugs.
FRONTIERS IN PUBLIC HEALTH
(2023)
Article
Biochemical Research Methods
Weidu Song, Chuanhao Zhang, Huichao Lin, Taiyi Zhang, Haixia Liu, Xiaowen Huang
Summary: The proposed real-time rotary PCR reaction system (RRP) meets the requirements of low cost, low-power consumption, potential for miniaturization, and portability. Experimental and theoretical results demonstrate its feasibility in the rapid quantitative detection of Pseudomonas aeruginosa and have broad application prospects in bacteria detection and food safety.
Article
Biochemical Research Methods
Shaolei Huang, Yiquan An, Bangchao Xi, Xianglian Gong, Zhongfu Chen, Shan Shao, Shengxiang Ge, Jun Zhang, Dongxu Zhang, Ningshao Xia
Summary: Nucleic acid detection is a method that directly identifies the presence of pathogenic microorganisms and has advantages of high sensitivity, commendable specificity, and a short window period. A real-time PCR system based on multiple temperature zones was proposed to solve the time-consuming problem, enabling fast detection speed and high sensitivity under extreme conditions.
Article
Multidisciplinary Sciences
Ayumi Koyama, Dai Miyazaki, Yuji Nakagawa, Yuji Ayatsuka, Hitomi Miyake, Fumie Ehara, Shin-ichi Sasaki, Yumiko Shimizu, Yoshitsugu Inoue
Summary: A deep learning-based algorithm was developed for computer-assisted diagnosis of infectious keratitis, showing high accuracy in distinguishing different pathogens and robustness even with low-resolution images.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Rebeca Alonso-Monge, Daniel Prieto, Ioana Coman, Sara Rochas, David M. Arana, Susana Hidalgo-Vico, Elvira Roman, Jesus Pla
Summary: Candida albicans is an important pathogen in human fungal diseases, with the gastrointestinal tract being a significant reservoir for its infections. Certain isolates of C. albicans have been identified to have enhanced fitness in a murine gastrointestinal commensalism model, even displaying colonization in mice without antibiotic treatment. These isolates could potentially facilitate the study of factors regulating fungal colonization.