Journal
SENSORS
Volume 15, Issue 1, Pages 1047-1059Publisher
MDPI
DOI: 10.3390/s150101047
Keywords
micro-Raman spectroscopy; AC electrokinetics; dielectrophoresis; microelectrodes; optical biosensors; M13 phage
Funding
- NSERC
- Queen's University
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A detection method that combines electric field-assisted virus capture on antibody-decorated surfaces with the fingerprinting capabilities of micro-Raman spectroscopy is demonstrated for the case of M13 virus in water. The proof-of-principle surface mapping of model bioparticles (protein coated polystyrene spheres) captured by an AC electric field between planar microelectrodes is presented with a methodology for analyzing the resulting spectra by comparing relative peak intensities. The same principle is applied to dielectrophoretically captured M13 phage particles whose presence is indirectly confirmed with micro-Raman spectroscopy using NeutrAvidin-Cy3 as a labeling molecule. It is concluded that the combination of electrokinetically driven virus sampling and micro-Raman based signal transduction provides a promising approach for time-efficient and in situ detection of viruses.
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