4.5 Article

Genotypic Characterization of Environmental Isolates of Cryptococcus gattii from Puerto Rico

Journal

MYCOPATHOLOGIA
Volume 170, Issue 4, Pages 279-285

Publisher

SPRINGER
DOI: 10.1007/s11046-010-9296-3

Keywords

C. gattii; Puerto Rico; Environmental isolates

Categories

Ask authors/readers for more resources

Cryptococcus neoformans and Cryptococcus gattii are found in distinct environments with some overlap around different parts of the world. However, no systematic surveys of these two pathogens have been reported from Puerto Rico, a tropical island uniquely situated between mainland USA and countries in South America. We carried out an exhaustive environmental survey in southwestern Puerto Rico for pathogenic Cryptococcus species. Twenty-two presumptive isolates of C. gattii from cacti and tree detritus were characterized in detail by physiological and molecular methods and seventeen strains were confirmed as C. gattii. Cryptococcus gattii isolates were haploid and MAT strains. Sixteen out of seventeen C. gattii isolates belonged to VGII/AFLP6 genotype while one isolate was a VGIV/AFLP7 genotype. The results are significant as Puerto Rico strains are distinct from VGIII/AFLP5 strains reported from Southern California, but similar to C. gattii VGII/AFLP6 molecular type implicated in recent outbreaks of cryptococcosis in Pacific Northwest and British Columbia, Canada, but different in its M13 fingerprinting, and a common genotype in South America.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.5
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

Article Immunology

Foodborne Botulism Outbreak Associated With Commercial Nacho Cheese Sauce From a Gas Station Market

Hilary E. Rosen, Akiko C. Kimura, John Crandall, Alyssa Poe, June Nash, Jason Boetzer, Selam Tecle, Rituparna Mukhopadhyay, Kate McAuley, Olivia Kasirye, Alvaro Garza, Mahtab Shahkarami, Vishnu Chaturvedi, David Kiang, Jeff Vidanes, Kelly McCoy, Mark Barcellos, Tammy Derby, Seema Jain, Duc J. Vugia

CLINICAL INFECTIOUS DISEASES (2020)

Article Chemistry, Medicinal

Unique Cell Surface Mannan of Yeast Pathogen Candida auris with Selective Binding to IgG

Lufeng Yan, Ke Xia, Yanlei Yu, Anna Miliakos, Sudha Chaturvedi, Fuming Zhang, Shiguo Chen, Vishnu Chaturvedi, Robert J. Linhardt

ACS INFECTIOUS DISEASES (2020)

Article Microbiology

Development of a Duplex Real-Time PCR Assay for the Differentiation of Blastomyces dermatitidis and Blastomyces gilchristii and a Retrospective Analysis of Culture and Primary Specimens from Blastomycosis Cases from New York (2005 to 2019)

Mitchell Kaplan, YanChun Zhu, Julianne Kus, Lisa McTaggart, Vishnu Chaturvedi, Sudha Chaturvedi

Summary: A highly sensitive, specific, and reproducible TaqMan duplex real-time PCR assay was developed to differentiate Blastomyces dermatitidis and Blastomyces gilchristii, revealing the distribution of these pathogens in blastomycosis cases in New York.

JOURNAL OF CLINICAL MICROBIOLOGY (2021)

Article Infectious Diseases

Real-time PCR assay for detection and differentiation of Coccidioides immitis and Coccidioides posadasii from culture and clinical specimens

Sudha Chaturvedi, Tanya R. Victor, Anuradha Marathe, Ketevan Sidamonidze, Kelly L. Crucillo, Vishnu Chaturvedi

Summary: Coccidioidomycosis (Valley Fever) is a fungal disease caused by two closely related pathogens: Coccidioides immitis and C. posadasii. The development of a TaqMan real-time PCR assay allows for rapid and accurate differentiation of the two pathogens, improving treatment management and surveillance of the disease.

PLOS NEGLECTED TROPICAL DISEASES (2021)

Article Microbiology

Antifungal Resistance Trends of Candida auris Clinical Isolates in New York and New Jersey from 2016 to 2020

Shannon Kilburn, Gabriel Innes, Monica Quinn, Karen Southwick, Belinda Ostrowsky, Jane A. Greenko, Emily Lutterloh, Rebecca Greeley, Reed Magleby, Vishnu Chaturvedi, Sudha Chaturvedi

Summary: Around 55% of U.S. Candida auris clinical cases were reported in New York and New Jersey from 2016 to 2020. Almost all clinical isolates from New York and New Jersey (99.8%) were resistant to fluconazole, and 50% were resistant to amphotericin B. Echinocandin resistance increased from 0% to 4%, and pan-resistance increased from 0 to C. auris clinical isolates, but not in New Jersey, highlighting regional differences.

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY (2022)

Article Microbiology

Reverse Transcription-Quantitative Real-Time PCR (RT-qPCR) Assay for the Rapid Enumeration of Live Candida auris Cells from the Health Care Environment

Bryanna Lexus Freitas, Lynn Leach, Vishnu Chaturvedi, Sudha Chaturvedi

Summary: A new detection method has been developed to rapidly detect live multidrug-resistant yeast Candida auris in healthcare environments. This method is highly sensitive and specific, and it correlates with the results from traditional culture methods.

JOURNAL OF CLINICAL MICROBIOLOGY (2022)

Article Virology

A Single-Round Infection Fluorescent SARS-CoV-2 Neutralization Test for COVID-19 Serological Testing at a Biosafety Level-2 Laboratory

Jing Zou, Hongjie Xia, Pei-Yong Shi, Xuping Xie, Ping Ren

Summary: A robust serological test to measure neutralizing antibodies against SARS-CoV-2 in BSL-2 laboratories has been developed and evaluated, showing high sensitivity and specificity compared to the gold standard test. The test is highly reproducible and can be used for large-scale testing of COVID-19 patients or vaccinated individuals.

VIRUSES-BASEL (2022)

Article Microbiology

Candida auris Pan-Drug-Resistant to Four Classes of Antifungal Agents

Samantha E. Jacobs, Jonathan L. Jacobs, Emily K. Dennis, Sarah Taimur, Meenakshi Rana, Dhruv Patel, Melissa Gitman, Gopi Patel, Sarah Schaefer, Kishore Iyer, Jang Moon, Victoria Adams, Polina Lerner, Thomas J. Walsh, YanChun Zhu, Mohammed Rokebul Anower, Mayuri M. Vaidya, Sudha Chaturvedi, Vishnu Chaturvedi

Summary: Candida auris isolates from a patient who underwent transplantation were analyzed for genome and drug resistance. The isolates showed global similarity and a distinct subcluster. Drug resistance was observed in some isolates, including those with unique mutations. The pan-drug-resistant isolates were also resistant to two-drug combination therapy. Genetic analysis revealed in-hospital evolution and circulation of the C. auris isolates.

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY (2022)

Article Virology

Vaccination Decreases the Infectious Viral Load of Delta Variant SARS-CoV-2 in Asymptomatic Patients

Jessica A. Plante, Rafael R. G. Machado, Brooke M. Mitchell, Divya P. Shinde, Jordyn Walker, Dionna Scharton, Allan McConnell, Nehad Saada, Jianying Liu, Bilal Khan, Rafael K. Campos, Bryan A. Johnson, Vineet D. Menachery, Corri B. Levine, Ping Ren, Susan L. F. McLellan, Kenneth S. Plante, Scott C. Weaver

Summary: The Delta variant of SARS-CoV-2 can cause breakthrough infections in fully vaccinated individuals. Vaccination reduces the infectious viral load and increases the levels of anti-spike IgA in the nasal secretions of asymptomatic individuals infected with the Delta variant.

VIRUSES-BASEL (2022)

Article Mycology

Utility of CHROMagar™ Candida Plus for presumptive identification of Candida auris from surveillance samples

Anuradha Marathe, YanChun Zhu, Vishnu Chaturvedi, Sudha Chaturvedi

Summary: Candida auris is an important nosocomial fungal pathogen, and its rapid identification using CHROMagar (TM) Candida Plus can aid in surveillance efforts to control its spread in healthcare settings.

MYCOPATHOLOGIA (2022)

Article Microbiology

Comparison of Analytical Sensitivity (Limit of Detection) of Xpert MTB/RIF and Xpert MTB/RIF Ultra for Non-Sputum Specimens

Marisa C. C. Nielsen, Paula Clarner, Ruchi Paroha, Sunhee Lee, Phyu M. M. Thwe, Ping Ren

Summary: Tuberculosis (TB) is a major public health threat and remains a leading cause of death worldwide. The Cepheid Xpert (R) MTB/RIF tests are widely accepted for TB diagnosis, but their sensitivity on non-sputum samples has not been determined. This study systematically evaluated the sensitivity of US-IVD and Ultra tests on different sample types including CSF, tissue, and BAL. The Ultra test showed better sensitivity than US-IVD across all sample types, providing a foundation for early detection of pulmonary and extrapulmonary TB and potentially improving TB case detection rates.

PATHOGENS (2023)

Article Microbiology

Development and Validation of TaqMan Chemistry Probe-Based Rapid Assay for the Detection of Echinocandin-Resistance in Candida auris

YanChun Zhu, Kelli M. Hager, Swati R. Manjari, Nilesh K. Banavali, Vishnu Chaturvedi, Sudha Chaturvedi

Summary: Candida auris is a multidrug-resistant yeast pathogen causing global outbreaks in health care facilities, and the emergence of echinocandin-resistant strain is concerning. The currently used antifungal susceptibility tests are slow and not scalable, limiting their effectiveness in surveillance. The development of a TaqMan chemistry probe-based fluorescence melt curve analysis allows for rapid and accurate detection of FKS1 mutations conferring echinocandin resistance, providing a potential solution to the urgent need for assessment of resistance.

JOURNAL OF CLINICAL MICROBIOLOGY (2023)

Article Mycology

Nomenclatural issues concerning cultured yeasts and other fungi: why it is important to avoid unneeded name changes

Andrey Yurkov, Artur Alves, Feng-Yan Bai, Kyria Boundy-Mills, Pietro Buzzini, Neza Cadez, Gianluigi Cardinali, Serge Casaregola, Vishnu Chaturvedi, Valerie Collin, Jack W. Fell, Victoria Girard, Marizeth Groenewald, Ferry Hagen, Chris Todd Hittinger, Aleksey Kachalkin, Markus Kostrzewa, Vassili Kouvelis, Diego Libkind, Xinzhan Liu, Thomas Maier, Wieland Meyer, Gabor Peter, Marcin Piatek, Vincent Robert, Carlos A. Rosa, Jose Paulo Sampaio, Matthias Sipiczki, Marc Stadler, Takashi Sugita, Junta Sugiyama, Hiroshi Takagi, Masako Takashima, Benedetta Turchetti, Qi-Ming Wang, Teun Boekhout

Summary: The accurate application of fungal names is crucial for scientific communication, clinical diagnostics, and regulatory compliance. Maintaining the stability of the taxonomic system and ensuring the traceability of nomenclatural changes are key for a broad range of users and taxonomists. Proper preservation of nomenclatural history and physical organisms representing names is essential to assure the unambiguous application of names.

IMA FUNGUS (2021)

Article Mycology

Fusarium: more than a node or a foot-shaped basal cell

P. W. Crous, L. Lombard, M. Sandoval-Denis, K. A. Seifert, H-J Schroers, P. Chaverri, J. Gene, J. Guarro, Y. Hirooka, K. Bensch, G. H. J. Kema, S. C. Lamprecht, L. Cai, A. Y. Rossman, M. Stadler, R. C. Summerbell, J. W. Taylor, S. Ploch, C. M. Visagie, N. Yilmaz, J. C. Frisvad, A. M. Abdel-Azeem, J. Abdollahzadeh, A. Abdolrasouli, A. Akulov, J. F. Alberts, J. P. M. Araujo, H. A. Ariyawansa, M. Bakhshi, M. Bendiksby, A. Ben Hadj Amor, J. D. P. Bezerra, T. Boekhout, M. P. S. Camara, M. Carbia, G. Cardinali, R. F. Castaneda-Ruiz, A. Celis, V Chaturvedi, J. Collemare, D. Croll, U. Damm, C. A. Decock, R. P. de Vries, C. N. Ezekiel, X. L. Fan, N. B. Fernandez, E. Gaya, C. D. Gonzalez, D. Gramaje, J. Z. Groenewald, M. Grube, M. Guevara-Suarez, V. K. Gupta, V Guarnaccia, A. Haddaji, F. Hagen, D. Haelewaters, K. Hansen, A. Hashimoto, M. Hernandez-Restrepo, J. Houbraken, V Hubka, K. D. Hyde, T. Iturriaga, R. Jeewon, P. R. Johnston, Z. Jurjevic, I Karalti, L. Korsten, E. E. Kuramae, I Kusan, R. Labuda, D. P. Lawrence, H. B. Lee, C. Lechat, H. Y. Li, Y. A. Litovka, S. S. N. Maharachchikumbura, Y. Marin-Felix, B. Matio Kemkuignou, N. Matocec, A. R. McTaggart, P. Mlcoch, L. Mugnai, C. Nakashima, R. H. Nilsson, S. R. Noumeur, I. N. Pavlov, M. P. Peralta, A. J. L. Phillips, J. Pitt, G. Polizzi, W. Quaedvlieg, K. C. Rajeshkumar, S. Restrepo, A. Rhaiem, J. Robert, V Robert, A. M. Rodrigues, C. Salgado-Salazar, R. A. Samson, A. C. S. Santos, R. G. Shivas, C. M. Souza-Motta, G. Y. Sun, W. J. Swart, S. Szoke, Y. P. Tan, J. E. Taylor, P. W. J. Taylor, P. Tiago, K. Z. Vaczy, N. van de Wiele, N. A. van der Merwe, G. J. M. Verkley, W. A. S. Vieira, A. Vizzini, B. S. Weir, N. N. Wijayawardene, J. W. Xia, M. J. Yanez-Morales, A. Yurkov, J. C. Zamora, R. Zare, C. L. Zhang, M. Thines

Summary: The paragraph lists multiple research institutions from around the world, covering various research fields such as microbiology, agriculture, plant protection, and food science.

STUDIES IN MYCOLOGY (2021)

Article Multidisciplinary Sciences

Soil microbiome dataset from Guanica dry forest in Puerto Rico generated by shotgun sequencing

Roberto G. Sotomayor-Mena, Carlos Rios-Velazquez

DATA IN BRIEF (2020)

No Data Available