Article
Biotechnology & Applied Microbiology
Xiao-Yang Jing, Feng-Min Li
Summary: An improved method for predicting cell wall lytic enzymes was proposed in this study, utilizing support vector machine and a set of features to address the crisis of antimicrobial resistance. By employing synthetic minority over-sampling technique for data balancing and feature selection, the method shows excellent performance in predicting cell wall lytic enzymes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Corinna Probst, Magnus J. Hallas-Moller, Johan O. Ipsen, Jacob S. Brooks, Karsten Andersen, Mireille Haon, Jean-Guy A. Berrin, Helle Martens, Connie A. Nichols, Katja Johansen, J. Andrew A. Alspaugh
Summary: Fungi adapt to environmental stress by changing their size, shape, or rate of cell division. This study focuses on the role of the CEL1 gene in the fungal pathogen Cryptococcus neoformans, which is involved in cell wall remodeling and adaptation to the host environment. The results indicate that CEL1 is required for stress response phenotypes and virulence in C. neoformans.
Article
Multidisciplinary Sciences
Hucheng Chang, Neus Gacias Amengual, Alexander Botz, Lorenz Schwaiger, Daniel Kracher, Stefan Scheiblbrandner, Florian Csarman, Roland Ludwig
Summary: This study demonstrates the activity of Lytic polysaccharide monooxygenase (LPMO) on wood cell walls and suggests that even low levels of hydrogen peroxide can support the enzymatic degradation of wood biomass. LPMO plays a synergistic role in fungal extracellular catabolism and industrial biomass degradation.
NATURE COMMUNICATIONS
(2022)
Review
Microbiology
Neil A. R. Cow, Megan D. Lenardon
Summary: The fungal cell wall is crucial for the growth and survival of fungi and is targeted by antifungal drugs and the immune system. The inner wall of most fungi is made up of chitin and beta-glucans, while the outer wall is composed of a variety of polymers and glycosylated proteins. Understanding the organization of fungal cell walls can lead to the development of diagnostics and drugs for combating fungal infections.
NATURE REVIEWS MICROBIOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Jinfang Zhang, Baoyue Zhu, Xiaojian Xu, Yihan Liu, Qinggang Li, Yu Li, Fuping Lu
Summary: Deletion of lytE, lytF, and cwlS genes individually or in combination can enhance cell growth, delay cell lysis, and increase cell wall thickness, leading to improved heterologous protein expression in Bacillus amyloliquefaciens-based cell factories.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biology
Anna Isabell Weaver, Laura Alvarez, Kelly M. Rosch, Asraa Ahmed, Garrett Sean Wang, Michael S. van Nieuwenhze, Felipe Cava, Tobias Dorr
Summary: The peptidoglycan cell wall is a crucial structure for bacteria, with the ability to determine cell shape and support survival. Peptidoglycan is a dynamic process that requires regulated synthesis and turnover. This study uncovers the physiological role of lytic transglycosylases (LTGs), a class of autolytic enzymes, and demonstrates their essentiality for bacterial survival. The study also suggests that LTGs prevent toxic accumulation of peptidoglycan polymers and their function is independent of peptidoglycan synthesis.
Article
Multidisciplinary Sciences
Surya D. Aggarwal, Adrian J. Lloyd, Saigopalakrishna S. Yerneni, Ana Rita Narciso, Jennifer Shepherd, David Roper, Christopher G. Dowson, Sergio R. Filipe, N. Luisa Hiller
Summary: In response to unfavorable conditions, the Gram-positive pathogen Streptococcus pneumoniae relies on cell wall biosynthesis proteins MurM and MurN. MurM modulates stress response by decreasing the pool of mischarged tRNAs, while enhanced lysis in the absence of MurM may be caused by LytA.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Liyanage D. Fernando, Yordanis Perez-Llano, Malitha C. Dickwella Widanage, Anand Jacob, Liliana Martinez-Avila, Andrew S. Lipton, Nina Gunde-Cimerman, Jean-Paul Latge, Ramon Alberto Batista-Garcia, Tuo Wang
Summary: Halophilic fungi adapt to survive in hypersaline environments by altering their cell wall structures, which provide a mechanism to withstand external stress. These molecular principles can be utilized to optimize the use of halophilic strains in biotechnology applications.
NATURE COMMUNICATIONS
(2023)
Article
Microbiology
Xiaobo Zhong, Le Zhang, Gilles P. van Wezel, Erik Vijgenboom, Dennis Claessen
Summary: Lytic polysaccharide monooxygenases are commonly used in industry for efficient degradation of difficult-to-degrade polysaccharides. However, recently it has been discovered that these enzymes also play important biological roles, such as remodeling the bacterial cell wall. This newly identified biological role may be widespread due to the common presence of lytic polysaccharide monooxygenases in microbes.
Article
Chemistry, Multidisciplinary
Liyuan Zhang, Mengchen Zhang, Gongping Liu, Wanqin Jin, Xiaoyan Li
Summary: The article introduces a bio-utilization strategy to design a cell wall-graphene oxide microcomposite membrane for organic solvent nanofiltration. This membrane demonstrates a unique structure and shows high selectivity and permeance.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Yang Ye, Jian He, Hanle Wang, Wenbo Li, Qingya Wang, Chenqi Luo, Xiajing Tang, Xiaoyuan Chen, Xiuming Jin, Ke Yao, Min Zhou
Summary: This study reports a method to treat fungal keratitis using modified nanoparticles. These nanoparticles can disrupt the fungal cell wall and eradicate the fungi through the synergistic effects of ion-released chemotherapy, chemodynamic therapy, photodynamic therapy, and mild photothermal therapy. Additionally, a gel formulation of the nanoparticles can be used to topically treat infected corneas in a mouse model, killing the fungi, reducing inflammation, and promoting wound healing. The method shows good antifungal activity and biosafety, with potential for treating fungal keratitis.
Article
Biotechnology & Applied Microbiology
Amala Bhagwat, Fuming Zhang, Cynthia H. Collins, Jonathan S. Dordick
Summary: Lysostaphin (Lst) and endolysin PlyPH are potent modular lytic enzymes with activity against Staphylococcus aureus and Bacillus cereus, respectively. Their activity is reduced in the presence of bacterial growth-supporting conditions, such as complex media, which may limit their effectiveness in antimicrobial applications, therapeutics, and microbial control in more complex growth media.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Tingan Zhou, Shiyu Yu, Huibin Xu, Huiling Liu, Yijian Rao
Summary: Low production of fungal natural products (NPs) remains a critical factor in their large-scale applications. This study found that exogenous beta-glucanase treatment can stimulate fungal cell wall integrity, thereby improving the production of fungal NPs.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Katherine R. Hummels, Samuel P. Berry, Zhaoqi Li, Atsushi Taguchi, Joseph K. Min, Suzanne Walker, Debora S. Marks, Thomas G. Bernhardt
Summary: Gram-negative bacteria have a complex envelope that is a major factor in their inherent antibiotic resistance and drug entry. The coordination of the biogenesis of essential surface layers, such as peptidoglycan (PG) and outer membrane (OM), is still poorly understood. In this study, a regulatory interaction between the committed enzymes for PG and lipopolysaccharide (LPS) synthesis pathways in Pseudomonas aeruginosa was discovered.
Article
Biology
Luis F. Avila-Cobian, Stefania De Benedetti, Choon Kim, Rhona Feltzer, Matthew M. Champion, Jed F. Fisher, Shahriar Mobashery
Summary: The protein-interaction network of cell-wall biosynthesis assemblies in P. aeruginosa was elucidated using mass-spectrometry-based proteomics and binding assays. A key class of enzymes, the lytic transglycosylases (LTs), was identified and their putative binding partners were revealed through this pulldown strategy. Multiple binding partners were identified for the LTs, with a systematic assessment conducted for RlpA, a lipoprotein involved in bacterial daughter-cell separation.
COMMUNICATIONS BIOLOGY
(2022)
Article
Entomology
Adrian Wiater, Malgorzata Pleszczynska, Ewa Pietrykowska-Tudruj, Monika Janczarek, Bernard Staniec, Janusz Szczodrak
ENTOMOLOGICAL SCIENCE
(2019)
Review
Biochemistry & Molecular Biology
Magdalena A. Karas, Anna Turska-Szewczuk, Monika Janczarek, Agnieszka Szuster-Ciesielska
Article
Agronomy
Agnieszka Hanaka, Andrzej Plak, Piotr Zagorski, Ewa Ozimek, Anna Rysiak, Malgorzata Majewska, Jolanta Jaroszuk-Scisel
Article
Biochemistry & Molecular Biology
Mariola Andrejko, Anna Sieminska-Kuczer, Monika Janczarek, Ewa Janik, Miroslawa Bednarczyk, Mariusz Gagos, Malgorzata Cytrynska
ACTA BIOCHIMICA POLONICA
(2019)
Article
Biochemistry & Molecular Biology
Agnieszka Hanaka, Ewa Ozimek, Malgorzata Majewska, Anna Rysiak, Jolanta Jaroszuk-Scisel
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Chemistry, Medicinal
Katarzyna Dworaczek, Maria Kurzylewska, Magdalena A. Karas, Monika Janczarek, Agnieszka Pekala-Safinska, Anna Turska-Szewczuk
Article
Biochemistry & Molecular Biology
Jolanta Jaroszuk-Scisel, Renata Tyskiewicz, Artur Nowak, Ewa Ozimek, Malgorzata Majewska, Agnieszka Hanaka, Katarzyna Tyskiewicz, Anna Pawlik, Grzegorz Janusz
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Immunology
Marta Palusinska-Szysz, Agnieszka Szuster-Ciesielska, Monika Janczarek, Sylwia Wdowiak-Wrobel, Jurgen Schiller, Emilia Reszczynska, Wieslaw I. Gruszecki, Beate Fuchs
PATHOGENS AND DISEASE
(2019)
Article
Biochemistry & Molecular Biology
Jolanta Jaroszuk-Scisel, Artur Nowak, Iwona Komaniecka, Adam Choma, Anna Jarosz-Wilkolazka, Monika Osinska-Jaroszuk, Renata Tyskiewicz, Adrian Wiater, Jerzy Rogalski
Article
Multidisciplinary Sciences
Paulina Lipa, Monika Janczarek
Article
Veterinary Sciences
Piotr Wilkolek, Marcin Szczepanik, Beata Rodzik, Wieslaw Sitkowski, Michal Pluta, Iwona Taszkun, Marcin Golynski
JOURNAL OF EQUINE VETERINARY SCIENCE
(2020)
Article
Entomology
Katarzyna Buczek, Kamil Derylo, Mateusz Kutyla, Katarzyna Rybicka-Jasinska, Dorota Gryko, Grzegorz Borsuk, Beata Rodzik, Mariusz Trytek
Article
Agronomy
Magdalena Kachel, Artur Nowak, Jolanta Jaroszuk-Scisel, Renata Tyskiewicz, Stanislaw Parafiniuk, Fabienne Rabier
Summary: The study found that the application of CuNPs is more beneficial for the biological and biochemical activities of Brassica napus rhizospheric soil compared to the application of AgNPs. The abundance of all tested microbial groups showed a positive correlation with CuNPs but a negative correlation with AgNPs in each application version. Additionally, the impact of different NP concentrations on soil pH value and biochemical parameters varied between CuNPs and AgNPs.
Review
Horticulture
Agnieszka Hanaka, Ewa Ozimek, Emilia Reszczynska, Jolanta Jaroszuk-Scisel, Maria Stolarz
Summary: Increasing temperatures lead to intensified water evaporation, contributing to global warming and exacerbating drought stress, which triggers modifications in plant physiology and microbial functioning. Plants have developed strategies to cope with drought stress, with potential impacts on productivity and food security.
Article
Veterinary Sciences
Piotr Wilkolek, Marcin Szczepanik, Wieslaw Sitkowski, Beata Rodzik, Michal Pluta, Iwona Taszkun, Marcin Golynski
JOURNAL OF VETERINARY SCIENCE
(2019)