Article
Biochemistry & Molecular Biology
Halina Jurkowska, Maria Wrobel, Ewa Jasek-Gajda, Leszek Rydz
Summary: This study investigated the expression of sulfurtransferases and cystathionine beta-synthase in human leukemia cell lines. The results revealed significant differences in the mRNA and protein levels of these enzymes among different leukemia cells. These findings contribute to understanding the variations in sulfur compound metabolism and provide potential targets for inhibiting leukemic cell proliferation.
Review
Biochemistry & Molecular Biology
Marta Kaczor-Kaminska, Kamil Kaminski, Maria Wrobel
Summary: Mucopolysaccharidosis type IIIB is a rare disease caused by mutations in the N-alpha-acetylglucosaminidase gene, resulting in reduced or absent enzyme activity. The disorder is characterized by the accumulation of heparan sulfate in lysosomes. This study reveals that the degradation of heparan sulfate produces monosaccharides and inorganic sulfate, which disrupts the metabolism of L-cysteine and leads to elevated levels of L-cysteine and reduced antioxidant potential in cells.
Article
Cell Biology
Dominika Szlezak, Patrycja Bronowicka-Adamska, Tomasz Hutsch, Marcin Ufnal, Maria Wrobel
Summary: Hypertension and age were found to affect hepatic sulfur metabolism and the production of H2S in rats, with SHRs showing higher capacity for H2S generation. Differences in gene expression and enzyme activity related to sulfur metabolism were observed between WKY and SHR, as well as between different age groups of rats.
Review
Biochemistry & Molecular Biology
Brandan Pedre, Tobias P. Dick
Summary: 3-Mercaptopyruvate sulfurtransferase (MPST) is involved in hydrogen sulfide generation, protein modification, and cyanide detoxification, while also playing a crucial role in oxidative stress resistance, mitochondrial function, and fatty acid metabolism. However, the overall role and regulation of MPST in the broader physiological context remain to be fully understood.
BIOLOGICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Prerona Bora, Suman Manna, Mrutyunjay A. Nair, Rupali R. M. Sathe, Shubham Singh, Venkata Sai Sreyas Adury, Kavya Gupta, Arnab Mukherjee, Deepak K. Saini, Siddhesh S. Kamat, Amrita B. Hazra, Harinath Chakrapani
Summary: Persulfides and polysulfides, along with hydrogen sulfide, are crucial in cellular physiology and disease, and enhancing these species through artificial substrates presents a promising therapeutic approach. By utilizing the enzyme machinery in cells, particularly 3-MST, researchers have successfully demonstrated the generation of persulfides in vitro and in vivo, with potential applications in mitigating oxidative stress and inflammation.
Review
Biochemistry & Molecular Biology
Leszek Rydz, Maria Wrobel, Halina Jurkowska
Summary: Mitochondria are crucial organelles for the synthesis of Fe-S clusters, with sulfurtransferases playing a key role. Fe-S clusters are essential for Fe-S proteins, susceptible to oxidative stress but capable of being resynthesized or repaired. Sulfurtransferases are involved in the formation, maturation, and reconstitution of Fe-S clusters.
Article
Biochemistry & Molecular Biology
Brandan Pedre, Deepti Talwar, Uladzimir Barayeu, Danny Schilling, Marcin Luzarowski, Mikolaj Sokolowski, Sebastian Glatt, Tobias P. Dick
Summary: Protein S-persulfidation (P-SSH) is a common posttranslational modification that occurs under basal conditions and is often elevated under stress conditions. The mechanism by which proteins are persulfidated inside cells has remained unclear. This study identifies 3-mercaptopyruvate sulfur transferase (MPST) as a protein that directly engages in protein-to-protein transpersulfidation reactions, promoting cytoprotective functions through protein persulfidation.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Mingxue Ran, Qingbin Li, Yufeng Xin, Shaohua Ma, Rui Zhao, Min Wang, Luying Xun, Yongzhen Xia
Summary: Heterotrophic bacteria and human mitochondria use sulfide: quinone oxidoreductase (SQR) and persulfide dioxygenase (PDO) to oxidize sulfide to sulfite and thiosulfate. RHOD domains play multiple roles in sulfide oxidation by promoting thiosulfate formation and facilitating the reaction between inorganic polysulfide and glutathione. The fused and dissociated forms of the DUF442 domain modulate the activity of SQRCp and PDOCp, respectively, affecting the accumulation of cellular sulfane sulfur and the production of thiosulfate.
Article
Biochemistry & Molecular Biology
Malgorzata Iciek, Magdalena Gorny, Magdalena Kotanska, Anna Bilska-Wilkosz, Marta Kaczor-Kaminska, Jacek Zagajewski
Summary: The study aimed to investigate the mechanism of action of yohimbine and its relationship with reactive sulfur species formed during cysteine catabolism. Administration of yohimbine at a dose of 5 mg/kg/day reduced oxidative stress and normalized sulfate levels in obese rats. Therefore, yohimbine may serve as a potential therapeutic agent for obesity and liver injury.
Article
Biochemistry & Molecular Biology
Qiaoli Yu, Mingxue Ran, Yuping Xin, Huaiwei Liu, Honglei Liu, Yongzhen Xia, Luying Xun
Summary: Hydrogen sulfide (H2S) and its oxidation product zero-valent sulfur (S-0) are essential in animals, plants, and bacteria. The conversion of thiosulfate to cellular sulfane sulfur in Escherichia coli is mediated by the enzyme PspE, which increases cellular sulfane sulfur levels and protects against the toxicity of hydrogen peroxide. These findings provide insights for the use of thiosulfate as a donor of H2S and sulfane sulfur in human and animal tests.
Article
Biology
Konrad Kaleta, Anna Misterka, Leszek Rydz, Maria Wrobel, Halina Jurkowska
Summary: Cyanogenic glycosides are secondary metabolites widely found in plants consumed by humans and animals. Thiosulfate sulfurtransferase (TST) and 3-mercaptopyruvate sulfurtransferase (MPST) are enzymes involved in defense against cyanide toxicity and sulfane sulfur production. The study investigated the expression, activity, and sulfane sulfur levels in chicken tissues. The highest TST and MPST expression/activity was found in the liver, correlated with high sulfane sulfur levels. The expression/activity and sulfane sulfur levels were significantly lower in the gizzard compared to the liver and heart. TST and MPST enzymes play an important role in cyanide detoxification in chicken tissues, requiring appropriate sulfane sulfur levels and enzyme activity for tissue protection.
Article
Biology
Chao Tang, Jingjing Li, Yuemeng Shen, Menghui Liu, Honglei Liu, Huaiwei Liu, Luying Xun, Yongzhen Xia
Summary: Bacillus licheniformis MW3 has the ability to degrade bird feathers, which are rich in hazardous sulfur compounds. The bacterium has a gene cluster that helps it detoxify these compounds. The cluster contains 11 genes, including a repressor gene, two regulatory genes, and two genes that encode enzymes for sulfur oxidation. Together, these genes enable the bacterium to grow on feather and highlight the importance of removing sulfide and sulfane sulfur during feather degradation.
COMMUNICATIONS BIOLOGY
(2023)
Review
Pharmacology & Pharmacy
Huijie Zhao, Yanting Zhang, Xiaodi Fu, Chaoren Chen, Saadullah Khattak, Honggang Wang
Summary: Hepatocellular carcinoma (HCC) is the most common primary malignant tumor of the liver, and it is also the leading cause of cancer-related death worldwide. Despite numerous studies conducted on HCC, the molecular mechanisms and effective treatment options are not fully understood. Hydrogen sulfide (H2S), previously considered a toxic gas, has been found to have multiple biological functions, but its role and mechanism in HCC remain unclear.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Dominika Szlezak, Tomasz Hutsch, Marcin Ufnal, Maria Wrobel
Summary: This study evaluated the influence of hypertension and aging on sulfur-containing compounds metabolism in the hearts and kidneys. The results showed that hypertension and aging reduce hydrogen sulfide production and affect enzyme activity and gene expression in the heart and kidneys.
Review
Biochemistry & Molecular Biology
Swetha Pavani Rao, Prakashkumar Dobariya, Harshini Bellamkonda, Swati S. More
Summary: 3-mercaptopyruvate sulfurtransferase (3-MST) is an important enzyme involved in the production of hydrogen sulfide. It is conserved across species and found in both mitochondria and cytoplasm. In addition to other enzymes, 3-MST mediates the production of hydrogen sulfide, which has been shown to alleviate various illnesses. While the role of other enzymes in hydrogen sulfide biogenesis is well-documented, there is limited information on the 3-MST pathway. This article compiles current knowledge on the physiological and pathological roles of 3-MST, and explores its potential as a therapeutic target.
Article
Genetics & Heredity
Andrzej Doniec, Wojciech Luczak, Maria Wrobel, Milosz Janula, Andrzej Ossowski, Pawel Grzmil, Tomasz Kupiec
Summary: DNA testing in cases of disputed paternity is a routine analysis carried out in genetic laboratories to determine genetic similarities between alleged fathers and children. Analysis showed that polymorphic loci on chromosome 2 were used to exclude the alleged father's paternity. While the results did not exclude the defendant's biological paternity, they indicated a case of uniparental maternal disomy.
Article
Cell Biology
Dominika Szlezak, Patrycja Bronowicka-Adamska, Tomasz Hutsch, Marcin Ufnal, Maria Wrobel
Summary: Hypertension and age were found to affect hepatic sulfur metabolism and the production of H2S in rats, with SHRs showing higher capacity for H2S generation. Differences in gene expression and enzyme activity related to sulfur metabolism were observed between WKY and SHR, as well as between different age groups of rats.
Article
Chemistry, Multidisciplinary
Malgorzata Janik-Hazuka, Kamil Kaminski, Marta Kaczor-Kaminska, Joanna Szafraniec-Szczesny, Aleksandra Kmak, Hassan Kassassir, Cezary Watala, Maria Wrobel, Szczepan Zapotoczny
Summary: Encapsulation of garlic oil compounds DADS and DATS in oil-core nanocapsules based on a derivative of hyaluronic acid has been shown to protect against oxidation and undesired interactions, while preserving their biological functions and anticancer activity. The encapsulation also inhibits lysis of red blood cell membrane and interactions with serum or digestive track components, indicating potential for enhanced therapeutic effects.
Article
Biochemistry & Molecular Biology
Halina Jurkowska, Maria Wrobel, Ewa Jasek-Gajda, Leszek Rydz
Summary: This study investigated the expression of sulfurtransferases and cystathionine beta-synthase in human leukemia cell lines. The results revealed significant differences in the mRNA and protein levels of these enzymes among different leukemia cells. These findings contribute to understanding the variations in sulfur compound metabolism and provide potential targets for inhibiting leukemic cell proliferation.
Article
Biochemistry & Molecular Biology
Marta Kaczor-Kaminska, Kamil Kaminski, Maria Wrobel
Summary: This paper provides information on the activity and expression levels of three sulfurtransferases in various cell lines and their correlation with cellular antioxidant capacity. By analyzing the genetic variation of sulfurtransferases, the study reveals a large number of polymorphisms. Despite challenges, understanding sulfurtransferases could enhance disease comprehension and potentially lead to new treatment approaches.
Review
Biochemistry & Molecular Biology
Leszek Rydz, Maria Wrobel, Halina Jurkowska
Summary: Mitochondria are crucial organelles for the synthesis of Fe-S clusters, with sulfurtransferases playing a key role. Fe-S clusters are essential for Fe-S proteins, susceptible to oxidative stress but capable of being resynthesized or repaired. Sulfurtransferases are involved in the formation, maturation, and reconstitution of Fe-S clusters.
Article
Materials Science, Multidisciplinary
Anna Bentke-Imiolek, Kinga Kaszuba, Patrycja Bronowicka-Adamska, Barbara Czopik, Joanna Zarzecka, Maria Wrobel
Summary: The aim of this study was to determine the cytotoxic concentrations and incubation times of the dental adhesive system OptiBond Solo Plus in its non-polymerized form and investigate its relationship with oxidative stress. The results showed a decrease in cell number, changes in the expression of specific sulfur enzymes, and alterations in the levels of reduced and oxidized glutathione.
Review
Biochemistry & Molecular Biology
Marta Kaczor-Kaminska, Kamil Kaminski, Maria Wrobel
Summary: Mucopolysaccharidosis type IIIB is a rare disease caused by mutations in the N-alpha-acetylglucosaminidase gene, resulting in reduced or absent enzyme activity. The disorder is characterized by the accumulation of heparan sulfate in lysosomes. This study reveals that the degradation of heparan sulfate produces monosaccharides and inorganic sulfate, which disrupts the metabolism of L-cysteine and leads to elevated levels of L-cysteine and reduced antioxidant potential in cells.
Article
Biochemistry & Molecular Biology
Dominika Szlezak, Marcin Ufnal, Adrian Drapala, Emilia Samborowska, Maria Wrobel
Summary: A study found that nitric oxide (NO) plays an important role in regulating the circulatory system. Decreased NO availability is associated with hypertension, cardiovascular, and kidney diseases. The study also revealed that hypertension and aging decrease tissue NO levels and are associated with reduced urinary excretion of NOS inhibitors, namely ADMA and SDMA.
Article
Biochemistry & Molecular Biology
Leszek Rydz, Maria Wrobel, Klaudia Janik, Halina Jurkowska
Summary: This study investigates the expression of proteins involved in L-cysteine metabolism and antioxidative processes, as well as cell proliferation under hypoxic conditions in human melanoma cell lines. The results suggest that the inhibition of melanoma cell proliferation under hypoxia is associated with decreased expression of thioredoxin reductase-1 and cystathionine beta-synthase, which may have significance in melanoma treatment. Interestingly, it was found that the expression and activity of 3-mercaptopyruvate sulfurtransferase is significantly higher in metastatic melanoma cells than in primary melanoma cells under normoxia.
Meeting Abstract
Urology & Nephrology
Delaney Orcutt, Maria M. Wrobel, Sofiane Mrah, J. Peter Marquardt, Florian Fintelmann, Duncan Hussey, Evan Gross, Elliott Trott, Joel Kramer, Kaylee Oliva, John Gore, George R. Schade, Dan Lin, Yaw Nyame, Scott Tykodi, John Thompson, Evan Hall, Ryan O'Malley, Sarah Psutka
JOURNAL OF UROLOGY
(2021)
