High-Throughput Assay of Levansucrase Variants in Search of Feasible Catalysts for the Synthesis of Fructooligosaccharides and Levan

Mutational analysis of conserved regions harboring catalytic triad residues of the levansucrase protein encoded by thelsc-3gene (lsc3) ofPseudomonas syringaepv. tomato DC3000

(2013) Karin Mardo et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

Sequence-based analysis of the microbial composition of water kefir from multiple sources

(2013) Alan J. Marsh et al.   FEMS MICROBIOLOGY LETTERS

Biomolecular Characterization of the Levansucrase of Erwinia amylovora, a Promising Biocatalyst for the Synthesis of Fructooligosaccharides

(2013) Lorenzo Caputi et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Crystal structure of a lactosucrose-producing enzyme, Arthrobacter sp. K-1 β-fructofuranosidase

(2012) Takashi Tonozuka et al.   ENZYME AND MICROBIAL TECHNOLOGY

Repression vs. activation ofMOX,FMD,MPP1andMAL1promoters by sugars inHansenula polymorpha: the outcome depends on cell's ability to phosphorylate sugar

(2012) Sandra Suppi et al.   FEMS YEAST RESEARCH

How glycan metabolism shapes the human gut microbiota

(2012) Nicole M. Koropatkin et al.   NATURE REVIEWS MICROBIOLOGY

Screening β-Fructofuranosidases Mutant Libraries to Enhance the Transglycosylation Rates of β-(2→6) Fructooligosaccharides

(2011) Miguel A. de Abreu et al.   COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING

Levansucrases from Pseudomonas syringae pv. tomato and P. chlororaphis subsp. aurantiaca: Substrate specificity, polymerizing properties and usage of different acceptors for fructosylation

(2011) Triinu Visnapuu et al.   JOURNAL OF BIOTECHNOLOGY

Single amino acid residue changes in subsite −1 of levansucrase from Zymomonas mobilis 10232 strongly influence the enzyme activities and products

(2010) Shuying Li et al.   MOLECULAR BIOLOGY REPORTS

The pURI family of expression vectors: A versatile set of ligation independent cloning plasmids for producing recombinant His-fusion proteins

(2010) José Antonio Curiel et al.   PROTEIN EXPRESSION AND PURIFICATION

A new acetonitrile-free mobile phase method for LC–ELSD quantification of fructooligosaccharides in onion (Allium cepa L.)

(2010) Katherine Downes et al.   TALANTA

Structural insights into glycoside hydrolase family 32 and 68 enzymes: functional implications

(2009) Willem Lammens et al.   JOURNAL OF EXPERIMENTAL BOTANY

Fully automated chip-based negative mode nanoelectrospray mass spectrometry of fructooligosaccharides produced by heterologously expressed levansucrase fromPseudomonassyringaepv. tomato DC3000

(2009) Triinu Visnapuu et al.   RAPID COMMUNICATIONS IN MASS SPECTROMETRY

Hansenula polymorpha maltase gene promoter with sigma 70-like elements is feasible for Escherichia coli-based biotechnological applications: Expression of three genomic levansucrase genes of Pseudomonas syringae pv. tomato

(2008) Triinu Visnapuu et al.   PROCESS BIOCHEMISTRY

Donor substrate recognition in the raffinose-bound E342A mutant of fructosyltransferase Bacillus subtilis levansucrase

(2008) Guoyu Meng et al.   BMC STRUCTURAL BIOLOGY

Expression Vectors for Enzyme Restriction- and Ligation-Independent Cloning for Producing Recombinant His-Fusion Proteins

(2007) Blanca de las Rivas et al.   BIOTECHNOLOGY PROGRESS