Article
Biochemical Research Methods
Herbert J. Dias, Eduardo J. Crevelin, Vinicius Palaretti, Ricardo Vessecchi, Antonio E. M. Crotti
Summary: Through negative ion ESI accurate-mass tandem and sequential mass spectrometry, it was found that deprotonated DBNs produced more diagnostic product ions than protonated molecules, providing more information and avoiding the use of additives.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2021)
Article
Chemistry, Applied
Jiaqi Li, Maorong Jiang, JiaoRui Zhou, Junjie Ding, Zhimou Guo, Ming Li, Fei Ding, Wengang Chai, Jingyu Yan, Xinmiao Liang
Summary: Oligosaccharides are crucial components in mammalian milk, promoting gut microbiota colonization and protecting newborns from infections. Research on acidic oligosaccharides in rats and mice is limited, but the identification of new oligosaccharides in these species provides baseline information for biological and biomedical studies.
CARBOHYDRATE POLYMERS
(2021)
Article
Chemistry, Analytical
Fong-Fu Hsu
Summary: This study utilized HCD MS2 spectra to investigate the fragmentation processes of ceramide molecules, allowing for accurate definition of ceramide structures based on observed sets of fragment ions reflecting the fatty acyl chain and LCB. The approach permits differentiation of isomeric structures and detailed characterization of ceramide species in biological samples.
ANALYTICA CHIMICA ACTA
(2021)
Review
Pharmacology & Pharmacy
Manman Qin, Yuqing Qian, Lu Huang, Chao Zhong, Mingdong Li, Jun Yu, Huanwen Chen
Summary: Extraction electrospray ionization mass spectrometry (EESI-MS) enables effective ionization of trace molecules in complex samples without sample pretreatment, providing high sensitivity, selectivity, and response speed. It also allows for advanced functionalization of high-energy molecular species. This article systematically reviews the effects of operating conditions on the analytical performance of EESI-MS for various pharmaceutical compounds and introduces its potential application in molecular functionalization for drug discovery.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Jean-Arthur Amalian, Tathagata Mondal, Evgeniia Konishcheva, Gianni Cavallo, Benoit Eric Petit, Jean-Francois Lutz, Laurence Charles
Summary: Sequence-defined synthetic polymers are being used as a medium to store information at the molecular level, with low storage capacity of readable chains being a current limitation. Ordering short oligomers on surfaces to compose long messages is an attractive alternative, and desorption electrospray ionization (DESI) is shown to be suitable for bidimensional data storage.
ADVANCED MATERIALS TECHNOLOGIES
(2021)
Article
Biochemical Research Methods
Michael Goetze, Lukasz Polewski, Leila Bechtella, Kevin Pagel
Summary: This article introduces a 3D-printable nano-electrospray ionization (nano-ESI) source for Bruker instruments, which can be assembled by anyone with access to a 3D printer. The source can be fitted to any Bruker mass spectrometer with an ionBooster ESI source and only requires minor, reversible changes to the original Bruker hardware. The general utility of the source was demonstrated by recording high-resolution MS spectra of small molecules, intact proteins, as well as complex biological samples in negative and positive ion mode on two different Bruker instruments.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Multidisciplinary Sciences
Amanda Gisler, Kapil Dev Singh, Jiafa Zeng, Martin Osswald, Mo Awchi, Fabienne Decrue, Felix Schmidt, Noriane A. Sievi, Xing Chen, Jakob Usemann, Urs Frey, Malcolm Kohler, Xue Li, Pablo Sinues
Summary: Exhaled breath analysis provides valuable molecular-level information and has promising potential for precision medicine. However, the lack of validation in multicenter trials hinders the transition of breath tests into routine clinical practice. In this study, an interoperability framework was developed and applied, revealing a technical variability of approximately 20% and identifying a core signature of the human exhaled metabolome. The results showed high inter-subject variability for certain metabolic classes but relative stability in other regions, such as the TCA cycle. This framework and the overview of metabolic coverage will pave the way for future large-scale multicenter trials.
Article
Biochemical Research Methods
Felix-Levin Hormann, Simon Sommer, Sven Heiles
Summary: Phospholipids are crucial components of eukaryotic cell membranes and undergo structural changes during metabolic states. These structural variations are associated with disease states and specific organisms. This study demonstrates that all analyzed phospholipid classes form doubly charged lipid-metal ion complexes during electrospray ionization, which can be utilized for lipid class assignment and fatty acid identification. The use of water free methanol and divalent metal salts greatly enhances the abundance of these complexes. This method allows for the differentiation of fatty acid isomers and relative quantitation of these isomeric compounds.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Review
Chemistry, Analytical
Bin Hu, Zhong-Ping Yao
Summary: This article reviews the technical aspects and important applications of wooden-tip ESI-MS in various fields. Wooden-tip ESI-MS has shown significant achievements in the past 10 years and has the potential for further development and application.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Multidisciplinary
Xiaowei Song, Qingce Zang, Chao Li, Tianhao Zhou, Richard N. N. Zare
Summary: We developed an immunoassay-based desorption electrospray ionization mass spectrometry imaging (immuno-DESI-MSI) technique for visualizing functional macromolecules. Boronic acid mass tags (BMTs) were synthesized to label antibodies as MSI probes, which can be released from the antibody by cleaving the boronic ester bond under acidic conditions. The BMTs can work in both optical and MS imaging modes and can be readily identified due to the introduction of the boron element.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Editorial Material
Biochemistry & Molecular Biology
Rafal Franski, Monika Beszterda-Buszczak
Summary: Villalva et al. evaluated the potential of yarrow extract as a treatment for H. pylori infection. They used the agar-well diffusion bioassay to test the extract's antimicrobial activity. The extract was fractionated into two fractions containing polar phenolic compounds and monoterpenes and sesquiterpenes, respectively. The phenolic compounds were identified using HPLC-ESIMS, but there were some disputed product ions.
Article
Chemistry, Analytical
Xiaoping Zhang, Xiang Ren, Yuan Zhong, Konstantin Chingin, Huanwen Chen
Summary: The study detected ketones in exhaled breath using mass spectrometry and developed a fast, sensitive, noninvasive method for ketone analysis. Results showed higher levels of acetone in the breath of smokers compared to non-smokers.
Article
Biochemical Research Methods
Niklas Farke, Thorben Schramm, Andreas Verhuelsdonk, Johanna Rapp, Hannes Link
Summary: Flow-injection mass spectrometry (FI-MS) allows high sample-throughput metabolomics studies. However, FI-MS is susceptible to in-source modifications of analytes due to the direct injection of samples without prior chromatographic separation. In this study, we spiked authentic standards of 160 primary metabolites into an Escherichia coli metabolite extract and analyzed the spike-in samples using FI-MS. The results showed that FI-MS can detect a wide range of chemically diverse analytes within a short measurement time, but extensive in-source modifications were observed. We used mass differences and MS2 spectra to connect unknown m/z features to (de-)protonated metabolites, which explained a significant portion of all features.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Chemistry, Analytical
Fengjian Chu, Gaosheng Zhao, Wangyu Li, Wei Wei, Weiwei Chen, Zihan Ma, Zhan Gao, Nazifi Sani Shuaibu, Jikui Luo, Bingwen Yu, Hongru Feng, Yuanjiang Pan, Xiaozhi Wang
Summary: A microwave plasma torch-based ion/molecular reactor (MPTIR) has been developed for creating and monitoring rapid oxidation reactions without the addition of external oxidants or catalysts. The oxidation products from the MPTIR match those of the long-term environmental oxidative process, and two unreported environmental compounds were identified in actual water samples using this device.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Ibraam E. Mikhail, Masoomeh Tehranirokh, Andrew A. Gooley, Rosanne M. Guijt, Michael C. Breadmore
Summary: This review summarizes various sample clean-up and analyte enrichment strategies that can be directly coupled with ESI-MS and nano-ESI-MS for the analysis of biological fluids, including solid phase extraction, solid phase micro-extraction, slug flow micro-extraction/nano-extraction, and more.
JOURNAL OF CHROMATOGRAPHY A
(2021)
