Journal
MOLECULES
Volume 17, Issue 12, Pages 13813-13824Publisher
MDPI AG
DOI: 10.3390/molecules171213813
Keywords
lipase; transesterification; prodrug; immobilization of enzymes
Funding
- Institute of Organic Chemistry and Biochemistry [AS CR RVO: 61388963]
- Academy of Science of the Czech Republic [M200551203]
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Lipases from Geotrichum candidum 4013 (extracellular lipase and cell-bound lipase) were immobilized by adsorption on chitosan beads. The enzyme preparations were tested in the synthesis of ester prodrugs from racemic 9-(2,3-dihydroxypropyl) adenine in dimethylformamide with different vinyl esters (acetate, butyrate, decanoate, laurate, palmitate). The transesterification activities of these immobilized enzymes were compared with commercially available lipases (lipase from hog pancreas, Aspergillus niger, Candida antarctica, Pseudomonas fluorescens). Lipase from Candida antarctica was found to be the most efficient enzyme regarding chemical yield of the desired products, while transesterification by lipase from Aspergillus niger resulted in lower yields.
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