Article
Endocrinology & Metabolism
Nils Mueller, Juan Pablo Robles, Magdalena Zamora, Johannes Ebnet, Hulya Markl-Hahn, Gonzalo Martinez de la Escalera, Carmen Clapp, Thomas Bertsch, Jakob Triebel
Summary: Vasoinhibin, a protein hormone with key roles in vascular diseases, has been targeted for detection using monoclonal antibodies and ELISA, showing potential for diagnostic and therapeutic applications.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Materials Science, Multidisciplinary
Bo Kyeong Yoon, Tun Naw Sut, Ki Yeol Yoo, Seung Hwa Lee, Youngkyu Hwang, Joshua A. Jackman, Nam-Joon Cho
Summary: A streamlined ELISA strategy using lipid nanoparticles as a blocking agent was developed, showing fewer processing steps, quicker protocol time, and superior detection performance compared to conventional BSA blocking. This method has been successfully applied to COVID-19 antibody detection and is broadly applicable for rapid ELISA diagnostics development.
APPLIED MATERIALS TODAY
(2021)
Article
Immunology
Jesse Bodle, Kirsten Vandenberg, Karen Laurie, Ian G. Barr, Ying Zhang, Steven Rockman
Summary: This study aimed to determine the applicability of enzyme linked immunoassay (ELISA) in quantifying and assessing the stability of different influenza vaccines. The ELISA, utilizing stable-indicating monoclonal antibodies (mAbs), accurately measures the HA antigen content and stability, with higher sensitivity and specificity towards different influenza B virus strains. Moreover, the ELISA can accurately quantify HA from different platforms and processing stages. Therefore, it is a suitable replacement for the traditional single radial immunodiffusion (SRID) assay.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Food Science & Technology
Jiao Li, Yuan Ding, He Chen, Wanlin Sun, Yue Huang, Fengquan Liu, Minghua Wang, Xiude Hua
Summary: Propiconazole, a commonly used fungicide in agricultural production, presents health risks. An ic-ELISA method using monoclonal antibodies was developed for accurate detection of propiconazole residues, showing high sensitivity and reliability. The results were consistent with gas chromatography-mass spectrometry analysis, providing an alternative tool for propiconazole determination.
Article
Allergy
Azadeh Hadadianpour, Jacob Daniel, Jian Zhang, Benjamin W. Spiller, Asta Makaraviciute, Asa M. DeWitt, Heather S. Walden, Robert G. Hamilton, R. Stokes Peebles, Thomas B. Nutman, Scott A. Smith
Summary: This study generated human hybridomas secreting naturally occurring helminth-specific IgE mAbs from individuals with filarial infections. The research provides valuable insight into the immune response and IgE antibody response induced by parasitic worms.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Article
Chemistry, Applied
Long Xu, Fanfan Yang, Alberto C. P. Dias, Xiaoying Zhang
Summary: Sunset yellow is a commonly used food colorant, but excessive and illegal use may pose health risks. Researchers have applied quantum dots in the analysis of sunset yellow and developed two new detection methods. These methods are efficient, highly sensitive, and capable of distinguishing sunset yellow from analogues.
Article
Food Science & Technology
Xiya Zhang, Mingyue Ding, Chensi Zhang, Yexuan Mao, Youyi Wang, Peipei Li, Haiyang Jiang, Zhanhui Wang, Xuezhi Yu
Summary: The researchers developed a rapid icELISA method for monitoring brevetoxin levels in oysters, providing a useful tool for the detection of these toxins in shellfish samples.
Article
Chemistry, Applied
Shunsuke Fujii, Tomoe Ohta, Riho Ehama, Mizuki Irikida, Shuichi Nomura, Yukihiro Shoyama, Takuhiro Uto
Summary: This study developed a simple and reliable method for the detection and quantification of FMN in food samples, and successfully applied it in cell-based assays.
Article
Multidisciplinary Sciences
Youhee Heo, Kwanwoo Shin, Min Cheol Park, Ji Yoon Kang
Summary: This report introduces a method for enhancing the sensitivity of chemifluorescence-based ELISA, using photooxidation-induced fluorescence amplification (PIFA). PIFA utilizes autocatalytic photooxidation to amplify the fluorescent product, improving detection sensitivity. The method requires minimal modifications to the fluorescence reader and is compatible with conventional ELISA assays.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Erandi Munasinghe, Maheshi Athapaththu, Wimaladharma Abeyewickreme
Summary: Synthetic peptides were designed and synthesized as antigens for antibody production to detect dengue infection. Monoclonal antibodies generated from immunized mice showed significant reactivity with dengue NS1 protein, enabling the detection of dengue infection in urine samples.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Medicine, General & Internal
Agnieszka Lebek-Szatanska, Lucyna Papierska, Piotr Glinicki, Wojciech Zgliczynski
Summary: This study aimed to evaluate the diagnostic performance of new screening methods for primary aldosteronism (PA). The results showed that the aldosterone-to-angiotensin II ratio (AA2R) had significantly worse diagnostic performance compared to the aldosterone-to-direct renin ratio (ADRR) and was not effective as a screening tool for PA. These findings provide important evidence in the discussion on the accuracy of renin-angiotensin system evaluation by recently used ELISA tests.
Article
Biochemical Research Methods
Supaluk Krittanai, Rattanathorn Choonong, Khunkhang Butdapheng, Jakkrit Jabsanthia, Seiichi Sakamoto, Waraporn Putalun
Summary: This study aimed to develop an enzyme-linked immunosorbent assay (ELISA) using a glabridin-specific antibody. The glabridin-bovine serum albumin conjugates were injected into mice to produce hybridomas, and a highly specific antibody against glabridin was produced. The developed ELISA method showed high sensitivity and specificity for quantifying glabridin in plant materials and human serum samples.
PHYTOCHEMICAL ANALYSIS
(2023)
Article
Biochemical Research Methods
Nandini Debnath, Ludovic Saiveng Live, Mahla Poudineh
Summary: Biomarker detection in whole blood is important for understanding the disease. Current detection techniques have limitations, and there is a need for reliable and adaptable methods for point-of-care testing. We have developed a simple and low-cost protein detection device that can detect biomarkers in whole blood within 15 minutes. This technique has the potential to improve the diagnosis and screening of various diseases.
Article
Agriculture, Dairy & Animal Science
Aicha Yasmine Bellatreche, Riad Bouzid, Amandine Blaizot, Dominique Aubert, Radu Blaga, Khatima Ait-Oudhia, Delphine Le Roux
Summary: Toxoplasmosis is a zoonotic disease caused by the protozoan Toxoplasma gondii, affecting most warm-blooded animals. Assessing the seroprevalence of T. gondii in different animal species gives a good estimate of the global circulation of the parasite and the risk for human infections. This study compared two methods for detecting T. gondii antibodies in hunting dogs and found a high prevalence of infection. It emphasizes the importance of studying T. gondii infections in companion animals to assess the risk for human populations.
Review
Biochemistry & Molecular Biology
Shima Makaremi, Zahra Asadzadeh, Nima Hemmat, Amir Baghbanzadeh, Alessandro Sgambato, Farid Ghorbaninezhad, Hossein Safarpour, Antonella Argentiero, Oronzo Brunetti, Renato Bernardini, Nicola Silvestris, Behzad Baradaran
Summary: Immunotherapy is a promising approach in colorectal cancer (CRC) treatment, with checkpoint-blocking antibodies showing promising outcomes. The immunosuppressive tumor microenvironment is a cause of poor treatment response in CRC patients. Combination therapy with immune checkpoint inhibitors (ICIs) shows potential for enhancing immune responses against CRC tumors.
Review
Food Science & Technology
Shengtao Bo, Sui Kiat Chang, Hong Zhu, Yueming Jiang, Bao Yang
Summary: Prenylated stilbenoids are a unique class of natural phenolic compounds with potential health benefits, including antioxidant, anti-cancer, anti-inflammatory, and anti-microbial activities. This review provides a comprehensive overview of the structure, bioactivity, potential applications, edible sources, and biosynthesis strategies of prenylated stilbenoids in the food industry. The findings regarding their use as food preservatives, nutraceuticals, and food additives are discussed, providing a full image of these compounds.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Chemistry, Analytical
Zian Wang, Zhiwei Liu, Tian Guan, Xi Zeng, Runlin Shen, Zhaodong Li, Yi Lei, Zhenlin Xu, Zhili Xiao, Hongtao Lei, Dingqiang Huang
Summary: With the growing popularity of herbal tea in China, illegal drugs, such as aminopyrine, are being added by food fraudsters to enhance its effects. However, the current lack of immunoassays for aminopyrine makes real-time detection in the field difficult. In this study, a sensitive ic-ELISA method was developed using a polyclonal antibody for aminopyrine, which showed good consistency with LC-MS in detecting actual samples and can be considered as a reliable method for aminopyrine detection in herbal tea.
ANALYTICAL METHODS
(2023)
Review
Food Science & Technology
Mengting Chen, Aori Qileng, Hongzhi Liang, Hongtao Lei, Weipeng Liu, Yingju Liu
Summary: Mycotoxin contamination in foods and other goods is a major concern due to its toxicity, threat to public safety, and loss of resources. To address this, there is a need to develop simple, sensitive, inexpensive, and rapid platforms for mycotoxin detection. Immunoassays, particularly dual-mode immunosensors, are considered as promising methods for toxin detection. This review summarizes the types and detection principles of single-mode immunosensors and focuses on the development of dual-mode immunosensors for mycotoxin detection, as well as the combination of dual-mode immunosensors with portable devices for point-of-care testing. The challenges and future prospects of dual-mode immunosensors are also discussed to promote the advancement of rapid detection platforms.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2023)
Article
Food Science & Technology
Runlin Shen, Tian Guan, Zhaodong Li, Ziling Hong, Boris B. Dzantiev, Anatoly V. Zherdev, Anastasios Koidis, Xiaojun Yao, Hongtao Lei
Summary: In this study, a highly sensitive antibody to hydrochlorothiazide was obtained and a rapid detection method based on time resolved fluorescence was developed. The strip assay showed consistent results with the confirmed method and could be used to identify adulterated hydrochlorothiazide in functional foods.
Review
Food Science & Technology
Guoxing Jing, Yuanyuan Wang, Mengping Wu, Wenjie Liu, Shaofeng Xiong, Jianna Yu, Wenshan Li, Wen Liu, Yueming Jiang
Summary: Mycotoxins, highly toxic secondary metabolites produced by fungi, pose a serious threat to food safety and human health. Photocatalytic technology has shown great promise for the degradation of mycotoxins in food due to its high efficiency and low cost, particularly for aflatoxin and deoxynivalenol. This review provides an updated overview of the progress in photocatalytic degradation of mycotoxins in food, including the characteristics of new photocatalytic materials, degradation products, and potential mechanisms and pathways. It also offers a prospect for the future application of photocatalytic technology in mycotoxin degradation.
FOOD REVIEWS INTERNATIONAL
(2023)
Article
Agriculture, Multidisciplinary
Hong-Wei Liang, Bao-Zhu Jia, Wen-Feng Zhang, Xing-Xing Wang, Kai Zhou, Hong-Tao Lei, Zhen-Lin Xu, Lin Luo
Summary: In this study, a RF-ELISA method was successfully developed for the detection of tricaine. The detection limits of this method in tilapia and shrimp were determined to be 5.6 and 2.8 ng/g, respectively. The validation data from LC-MS/MS confirmed the accuracy, precision, and reliability of RF-ELISA. This method opens up new avenues for the analysis of tricaine and other targets in food safety detection.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Qiqi Quan, Zhiwei Liu, Zhaodong Li, Kangliang Pan, Anastasios Koidis, Yi Lei, Xiaoqin Yu, Qiuhua Mo, Xinan Huang, Hongtao Lei
Summary: A novel method was developed to quickly detect illegally used 6-benzylaminopurine (6-BA) in bean sprouts. Four immunizing haptens of 6-BA were designed using computer-assisted modeling and synthesized, leading to the production of a highly sensitive and specific antibody. The developed indirect competitive enzyme-linked immunosorbent assay (icELISA) showed a limit of detection of 0.075 μg/L for 6-BA, with average recoveries ranging from 87.2% to 95.0% and a coefficient of variation below 8.7%. The results obtained by icELISA were in good agreement with HPLC-MS/MS, demonstrating the effectiveness of the proposed method.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Review
Food Science & Technology
Wanli Zhang, Yueming Jiang, Zhengke Zhang
Summary: Fresh fruits have good flavor and high nutritional value, but they age and decay rapidly after harvest, requiring the use of green postharvest fruit preservatives. Natural organic acids are considered as promising options due to their safety and effectiveness. This review discusses the recent applications of various natural organic acids in postharvest fruit quality management, highlighting their potential biochemical mechanisms and benefits in delaying senescence, preventing chilling injury, controlling diseases, inhibiting browning, and improving overall fruit quality. Chemical modification and cotreatment strategies are also considered to enhance the efficiency of natural organic acid preservation methods.
Review
Food Science & Technology
Jiali Yang, Zhengjiao Gao, Zhiqian Yu, Yu Hou, Dingtao Tang, Huiling Yan, Fuwang Wu, Sui Kiat Chang, Yonggui Pan, Yueming Jiang, Zhengke Zhang, Bao Yang
Summary: This review summarizes the natural aurones isolated from dietary plants, a subclass of active flavonoids characterized by a scaffold of 2-benzylidene-3(2H)-benzofuranone. Their positive effects on immunomodulation, antioxidation, cancer prevention, and maintaining the health status of cardiovascular, nervous system, and liver organs are highlighted. The biosynthesis strategies of plant-derived aurones are elaborated to tackle their limited natural abundance, and the potential application of natural aurones in food coloration is discussed. This paper provides a comprehensive overview of dietary aurones, combining up-to-date information.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Chemistry, Applied
Yipeng Chen, Yueming Jiang, Lingrong Wen, Bao Yang
Summary: Pectin can improve the bioaccessibility of icaritin as a nanocarrier, while ultrasound can modify the pectin structure. This study investigated the effects of ultrasound-modified pectin (UMP) on the physiochemical properties of icaritin/pectin micelles (IPMs). The UMP-IPMs showed lower encapsulation efficiencies and loading capacities compared to native IPMs, but exhibited stronger anti-proliferation activities against HepG2 cells.
Article
Agriculture, Multidisciplinary
Jiayi Chen, Zehao Yang, Jianpeng Zhang, Xing Shen, Zhenlin Xu, Xiangmei Li, Hongtao Lei
Summary: In this study, the novel nanomaterial NU66@QD was synthesized and used to develop a multiple immunochromatographic assay (ICA) for the detection of multiple toxins in cereals and feed. The NU66@QD-ICA showed high sensitivity and efficiency, as confirmed by parallel analysis with LC-MS/MS.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Lin Ye, Huodai Chen, Karl Wah Keung Tsim, Xing Shen, Xiangmei Li, Xueling Li, Hongtao Lei, Yunle Liu
Summary: Through mouse experiments, it was found that the gut microbiota plays a crucial role in AFB(1)-induced liver injury, as alterations in gut microbiota lead to colonic barrier dysfunction and liver pyroptosis, resulting in hepatic inflammation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Food Science & Technology
Qiuxia Yang, Yingjun Liu, Yushan Guo, Yueming Jiang, Lingrong Wen, Bao Yang
Summary: This paper reviews the latest reports on nutrients and phytochemicals in fig, summarizes the health benefits and side effects reported in the last decade, and discusses the impacts of postharvest processing and variety selection on fig quality. Figs are rich in bioactive phytochemicals, with prenylated phenolics being rare and having good antioxidant, anticancer, and other activities. Sugars, organic acids, coumarins, and prenylated phenolics can be used as marker chemicals for variety selection.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2023)
Article
Food Science & Technology
Yanfei Wu, Qiuxiao Yang, Lihong Xie, Chunxiao Yin, Hongxia Qu, Junxian He, Yueming Jiang, Taotao Li
Summary: Fusarium proliferatum is a pathogenic fungus that causes crown rot in banana fruit during postharvest storage. The secreted aspartic proteinase FpOPSB plays a critical role in the infection process. FpOPSB enhances the virulence of F. proliferatum by suppressing plant immunity and promoting fungal growth.
Article
Plant Sciences
Guoxiang Jiang, Zhiwei Li, Xiaochun Ding, Yijie Zhou, Hongmei Lai, Yueming Jiang, Xuewu Duan
Summary: A WUSCHEL-related homeobox transcription factor (TF), SlWOX13, regulates tomato fruit ripening via ethylene synthesis and signaling, as well as transcriptional regulation of key ripening-related TFs.
