Article
Chemistry, Multidisciplinary
Lior Levy, Tzila Davidov, Krishna K. Kolluri, Avraham Fridman, Stasia Krishtul, Sam M. Janes, Marcelle Machluf
Summary: Utilizing immunotherapeutic-nano-ghosts (iNGs) for cancer cell therapy combines the advantages of mesenchymal stem cell (MSC) therapy and T cell immunotherapy, providing a powerful and effective treatment for malignant diseases.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Review
Biotechnology & Applied Microbiology
Miguel J. S. Ferreira, Fabrizio E. Mancini, Paul A. Humphreys, Leona Ogene, Michael Buckley, Marco A. N. Domingos, Susan J. Kimber
Summary: The use of human pluripotent stem cells in skeletal tissue engineering has shown promising results, with various methods being used for generating cartilage and bone. Emphasis is placed on generating authentic tissues rather than just differentiated cells, and new technologies are being developed to overcome challenges in this field. It is likely that cost-effective and efficacious engineered constructs using human pluripotent stem cells will be available for skeletal repair in the near future.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Shudong Zhao, Jishizhan Chen, Lei Wu, Xin Tao, Naheem Yaqub, Jinke Chang
Summary: Skeletal muscle, responsible for crucial functions in the body, can suffer from severe injuries leading to volumetric muscle loss (VML). Tissue-engineered muscles using induced pluripotent stem cells (iPSCs) offer a potential solution to restore muscle function. iPSCs, with their pluripotency and self-renewal capacity, can be used to construct tissue-engineered artificial skeletal muscles for various applications. These iPSC-based models also have the potential to revolutionize drug discovery and reduce reliance on animal models. This review provides an overview of iPSCs in tissue-engineered skeletal muscles, discussing advancements, challenges, and considerations for clinical translation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Jiesi Luo, Xiangyu Shi, Yuyao Lin, Yifan Yuan, Mehmet H. Kural, Juan Wang, Matthew W. Ellis, Christopher W. Anderson, Shang-Min Zhang, Muhammad Riaz, Laura E. Niklason, Yibing Qyang
Summary: The study successfully developed xenogeneic-free conditions for deriving functional endothelial cells from human induced pluripotent stem cells (XF-hiPSC-ECs), which exhibited similar marker expression and function to human primary ECs. Moreover, the XF-hiPSC-ECs responded to shear stress with typical cell alignment and gene expression, and were successfully used to endothelialize decellularized human vessels in a dynamic bioreactor system, advancing the potential clinical application of tissue engineered vascular grafts.
ACTA BIOMATERIALIA
(2021)
Article
Engineering, Biomedical
Shuai Deng, Xiaoyu Zhao, Yanlun Zhu, Ning Tang, Rongliang Wang, Xuerao Zhang, Fuyang Qu, Yi-Ping Ho, Wayne Yuk-Wai Lee, Jiansu Chen, Mingqiang Li, Yu Tao, Hon Fai Chan
Summary: Liver tissue engineering is advanced through the fabrication of collagen type I microspheres, enabling efficient hepatic differentiation of stem cells and assembly of prevascularized liver tissue. The microspheres exhibit high uniformity and can self-assemble with endothelial cells to form high cell-packing density liver tissue. This technology has potential applications in regenerative medicine, drug screening, and in vitro liver modeling.
Article
Engineering, Biomedical
Jiesi Luo, Yuyao Lin, Xiangyu Shi, Guangxin Li, Mehmet H. Kural, Christopher W. Anderson, Matthew W. Ellis, Muhammad Riaz, George Tellides, Laura E. Niklason, Yibing Qyang
Summary: Research successfully derived functional xenogeneic-free hiPSC-VSMCs by replacing animal-derived reagents with functional counterparts of human origin, and developed tissue rings with comparable mechanical strength. Engineered vascular tissues were generated by seeding XF-hiPSC-VSMCs onto biodegradable PGA scaffolds, suitable for implantation into an immunodeficient mice model.
ACTA BIOMATERIALIA
(2021)
Review
Cell & Tissue Engineering
Farnaz Sani, Mahsa Sani, Zahra Moayedfard, Maryam Darayee, Lobat Tayebi, Negar Azarpira
Summary: Liver damage caused by toxicity can lead to severe conditions such as acute liver failure, fibrogenesis, and cirrhosis, with liver cirrhosis being the leading cause of liver-related deaths globally. Transplantation of gene-engineered stem cells is a potential therapeutic approach to improve liver function. Genetic engineering enhances the regenerative potential of stem cells by releasing growth factors and cytokines. Further research is recommended to develop safe gene modification methods and ensure long-term patient follow-up to increase the effectiveness and reliability of these therapeutic strategies.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Endocrinology & Metabolism
Xin Wang, Mengxi Gao, Yali Wang, Yucheng Zhang
Summary: Diabetes is a complex metabolic disorder that requires new methods to restore pancreatic beta-cells. Pluripotent stem cells provide an ideal alternative source for generating these cells. This review systematically summarizes the progress, challenges, and future perspectives of using human pluripotent stem cells to treat diabetes.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Seongjun So, Yoonkyung Park, Soon Suk Kang, Jongsuk Han, Jeong Hye Sunwoo, Whanseo Lee, Jin Kim, Eun Ah Ye, Jae Yong Kim, Hungwon Tchah, Eunju Kang, Hun Lee
Summary: Corneal endothelial cells (CECs) cannot proliferate or recover, causing decreased cell density and loss of pump/barrier function. The study investigated the efficacy and safety of transplanting induced pluripotent stem cell (iPSC)-derived CECs into a corneal endothelial dysfunction (CED) rabbit model. Results showed successful differentiation of iPSCs into CECs and improved corneal transparency after transplantation. iPSC-derived CECs may be a promising cellular resource for treating CED.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Ying Zhang, Jun Wei, Jiani Cao, Kehua Zhang, Yaojin Peng, Hongkui Deng, Jiuhong Kang, Guangjin Pan, Yong Zhang, Boqiang Fu, Shijun Hu, Jie Na, Yan Liu, Lei Wang, Lingmin Liang, Huanxin Zhu, Yu Zhang, Zi-Bing Jin, Jie Hao, Aijin Ma, Tongbiao Zhao, Junying Yu
Summary: "Requirements for Human-Induced Pluripotent Stem Cells" is the first set of guidelines in China on human-induced pluripotent stem cells. It provides detailed technical requirements and aims to promote international standardization.
CELL PROLIFERATION
(2022)
Review
Cardiac & Cardiovascular Systems
Mengcheng Shen, Thomas Quertermous, Michael P. Fischbein, Joseph C. Wu
Summary: The developmental origin of vascular smooth muscle cells plays a crucial role in regional susceptibility and resistance to vascular diseases. Patient induced pluripotent stem cell-derived VSMCs are valuable for research and therapeutic applications, but the heterogeneity in developmental origins poses challenges.
CIRCULATION RESEARCH
(2021)
Article
Cell & Tissue Engineering
Ana Belen Alvarez-Palomo, Jordi Requena-Osete, Raul Delgado-Morales, Victoria Moreno-Manzano, Carme Grau-Bove, Agueda M. Tejera, Manel Juan Otero, Carme Barrot, Irene Santos-Barriopedro, Alejandro Vaquero, Jovita Mezquita-Pla, Sebastian Moran, Carlos Hobeich Naya, Iris Garcia-Martinez, Francisco Vidal Perez, Maria A. Blasco, Manel Esteller, Michael J. Edel
Summary: The study demonstrates that utilizing synthetic mRNA transfection of CYCLIN D1 during reprogramming repairs DNA and significantly improves the genetic stability of human iPSC. This method reduces various genetic instabilities, decreases DNA damage, promotes correct protein expression, and reduces single-nucleotide polymorphism changes per chromosome.
Article
Multidisciplinary Sciences
Yu Jin Jang, Mijeong Kim, Bum-Kyu Lee, Jonghwan Kim
Summary: The authors developed a simple procedure to convert human primed pluripotent stem cells into trophoblast stem-like cells using bone morphogenetic protein 4, providing an important opportunity to study human placenta development and pathology.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Medicine, General & Internal
Natsumi Ibano, Emi Inada, Shinji Otake, Yuki Kiyokawa, Kensuke Sakata, Masahiro Sato, Naoko Kubota, Hirofumi Noguchi, Yoko Iwase, Tomoya Murakami, Tadashi Sawami, Yoshito Kakihara, Takeyasu Maeda, Miho Terunuma, Yutaka Terao, Issei Saitoh
Summary: This study aimed to elucidate the role of genetically modified human feeder cells expressing growth factors in maintaining the integrity of primary cultured human deciduous dental pulp cells. The results showed that co-culturing enhanced cell proliferation, promoted differentiation, and maintained stemness properties.
JOURNAL OF CLINICAL MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Tianyuan Shi, Martin Cheung
Summary: Using rodents for modeling neurological diseases has limitations due to differences in genetics, neural development, and physiology between humans and rodents. In the past decade, induced pluripotent stem cells and induced neural stem cells generated through reprogramming somatic cells have offered a powerful alternative for studying disease pathogenesis and testing regenerative medicines. Urine-derived stem cells are an ideal cell source for reprogramming due to their proliferation, multipotency, epithelial nature, and ease of reprogramming.
CELL AND BIOSCIENCE
(2021)
