Article
Cardiac & Cardiovascular Systems
Sabin J. Bozso, Jimmy J. H. Kang, Ryaan El-Andari, Nicholas Fialka, Lin Fu Zhu, Steven R. Meyer, Darren H. Freed, Jayan Nagendran, Jeevan Nagendran
Summary: Autologous mesenchymal stem cell recellularization of xenogenic valves reduces the xenoreactive immune response in an in vivo rat model and may be an effective approach to decrease the progression of xenograft valve dysfunction.
EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY
(2022)
Article
Engineering, Biomedical
Bin Wang, Wei Liu, Jiao Jiao Li, Senlin Chai, Dan Xing, Hongsheng Yu, Yuanyuan Zhang, Wenjin Yan, Zhihong Xu, Bin Zhao, Yanan Du, Qing Jiang
Summary: In the treatment of osteoarthritis, delivering low doses of MSCs through microcarriers can achieve similar reparative effects as repeated high doses. Mechanistic investigations reveal that 3D growth of MSCs reduces inflammatory factors, enhances early chondrogenesis and extracellular matrix interactions, and maintains the phenotype of co-cultured chondrocytes.
BIOACTIVE MATERIALS
(2022)
Article
Materials Science, Ceramics
Patricia Ros-Tarraga, Carlos M. Martinez, Miguel A. Rodriguez, Piedad N. De Aza
Summary: The elderly population is increasing, leading to an increase in bone and joint problems. Scaffold for bone tissue regeneration can solve these problems, and material selection is crucial for satisfactory results. This study designed a three-dimensional porous ceramic scaffold and analyzed its biocompatibility and osteoinductive capacity. The results suggest that it has potential applications as bone substitutes.
CERAMICS INTERNATIONAL
(2022)
Article
Biotechnology & Applied Microbiology
Arturo Ibanez-Fonseca, Ana Rico, Silvia Preciado, Fernando Gonzalez-Perez, Sandra Muntion, Jesus Garcia-Brinon, Maria-Carmen Garcia-Macias, Jose Carlos Rodriguez-Cabello, Miguel Pericacho, Matilde Alonso, Fermin Sanchez-Guijo
Summary: Combination of an elastin-like recombinamer (ELR)-based hydrogel with mesenchymal stromal cells (MSCs) shows potential in improving tissue reperfusion and blood vessel density in a murine hindlimb ischemia model, suggesting a promising approach for clinical application.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Madison Moss, Mariela Pauli, Joshua M. Moreau, Jarish N. Cohen, Michael D. Rosenblum, Margaret M. Lowe
Summary: The human skin xenograft model is an important option for translational research in skin immunology. This paper provides an optimized technique for transplanting human skin onto mice and discusses necessary considerations for downstream experimental aims. Adherence to these methods allows for maintenance of xenografts for over 6 weeks post-surgery, facilitating experimental characterization of human skin and preclinical testing of compounds in vivo.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Engineering, Biomedical
Melissa Alvarado-Velez, Syed Faaiz Enam, Nalini Mehta, Johnathan G. Lyon, Michelle C. LaPlaca, Ravi V. Bellamkonda
Summary: The development of immunosuppressive hydrogels for stem cell delivery, such as the agarose-FasL hydrogel in this study, has shown promising results in enhancing the benefits of stem cell therapy for traumatic brain injury. By reducing host cytotoxic CD8(+) T cell populations, increasing the survival of transplanted allogeneic MSCs, and elevating the expression of neurotrophic factors in the injury penumbra, this approach holds potential for improving therapeutic outcomes in TBI cases.
Article
Nanoscience & Nanotechnology
Marine Z. C. Hatit, Melissa P. Lokugamage, Curtis N. Dobrowolski, Kalina Paunovska, Huanzhen Ni, Kun Zhao, Daryll Vanover, Jared Beyersdorf, Hannah E. Peck, David Loughreyl, Manaka Sato, Ana Cristianl, Philip J. Santangelo, James E. Dahlman
Summary: This study compared the delivery of mRNA by lipid nanoparticles in different species using DNA barcoding technology and identified species-dependent responses. These findings contribute to the acceleration of RNA therapy development.
NATURE NANOTECHNOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Ruyin Lyu, Yuping Chen, Yajun Shuai, Jie Wang, Leihao Lu, Qichao Cheng, Jiangfeng Cai, Chuanbin Mao, Mingying Yang
Summary: We demonstrate a universal strategy for discovering peptides that can bind to and functionalize biomaterials using a Bombyx mori silk fibroin membrane as a model. By fusing two peptides, one screened for binding to the biomaterial and another derived from a growth factor, a new chimeric peptide is created to efficiently bind to the silk fibroin membrane. The chimeric peptide enables the membrane to induce osteogenic differentiation of human mesenchymal stem cells and stimulate bone regeneration in a rat model without causing inflammation.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Cell Biology
Tatiana Borisovna Bukharova, Irina Alekseevna Nedorubova, Viktoria Olegovna Mokrousova, Anastasiia Yurevna Meglei, Viktoriia Pavlovna Basina, Andrey Anatolevich Nedorubov, Andrey Vyacheslavovich Vasilyev, Timofei Evgenevich Grigoriev, Yuriy Dmitrievich Zagoskin, Sergei Nicolaevich Chvalun, Sergey Ivanovich Kutsev, Dmitry Vadimovich Goldshtein
Summary: Adenovirus-mediated gene therapy is a promising approach in bone regenerative medicine. This study compared the effectiveness of in vivo and ex vivo gene therapy using adenoviral constructs with the BMP2 gene, PLA particles, and a fibrin clot for bone defect healing. The results showed that ex vivo gene therapy was more effective in promoting bone regeneration, indicating that ex vivo adenoviral gene therapy with the BMP2 gene is a more efficient approach for bone regeneration.
Article
Cell & Tissue Engineering
Yong-Hong Wang, Ya-Chao Tao, Dong-Bo Wu, Meng-Lan Wang, Hong Tang, En-Qiang Chen
Summary: Different storage solutions did not significantly affect cell behavior, while heterogeneity was prevalent in MSC populations and could limit cell application. Therefore, establishing a more precise standard for culture-expanded MSCs is necessary.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Marine & Freshwater Biology
Yali Luo, Wen-Xiong Wang
Summary: Oysters are excellent biomonitor of coastal pollution and can accumulate toxic metals such as copper and zinc. This study investigated the immune responses of different subpopulations of oyster hemocytes under different periods of zinc exposure, revealing temporal changes in immune modulation in response to zinc exposure.
AQUATIC TOXICOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yo-Han Han, Ji-Ye Kee
Summary: This study confirmed the anti-adipogenic effect of Isatidis Radix (IR) by inhibiting lipid accumulation in cells and a high-fat diet-induced obese mouse model. IR suppressed reactive oxygen species production, inhibited the expression of key genes involved in lipid synthesis and oxidation, and reduced weight gain and fat accumulation. Furthermore, IR extract decreased serum levels of free fatty acids, total cholesterol, and LDL cholesterol, suggesting its potential as an anti-obesity drug.
Review
Immunology
Yasunari Matsuzaka, Ryu Yashiro
Summary: This article summarizes the roles of extracellular vesicles produced by various immune cells, focusing on the importance of DCs and cancer cell-derived EVs, as well as the potential clinical applications of using EVs in tumor vaccination therapy.
Article
Chemistry, Multidisciplinary
Xue-Han Xu, Tie-Jun Yuan, Haseeb Anwar Dad, Mu-Yang Shi, Yi-Yu Huang, Zhi-Hong Jiang, Li-Hua Peng
Summary: Exosomes derived from ginseng were shown to efficiently promote neural differentiation of BMSCs by transferring miRNAs, demonstrating potential for neural regenerative medicine both in vitro and in vivo.
Article
Cell & Tissue Engineering
Bing Wei, Mingliang Ji, Yucheng Lin, Shanzheng Wang, Yuxi Liu, Rui Geng, Xinyue Hu, Li Xu, Zhuang Li, Weituo Zhang, Jun Lu
Summary: This study confirms that MSCs protect damaged tenocytes by transferring mitochondria. The transferred mitochondria can reduce cell death and oxidative stress, and promote cell proliferation and mitochondrial function restoration. In vivo experiments show that mitochondrial transfer from MSCs improves the expression of tendon-specific markers, decreases the infiltration of inflammatory cells, and facilitates tendon tissue remodeling.
STEM CELL RESEARCH & THERAPY
(2023)