Article
Developmental Biology
Zvi Roth
Summary: High temperatures can affect follicles and oocytes, leading to impaired follicle development and compromised competence of oocytes to develop into embryos. Studies have shown various mechanisms of oocyte damage due to heat stress, with only subpopulations of follicles affected rather than the entire ovarian reserve.
REPRODUCTION FERTILITY AND DEVELOPMENT
(2021)
Article
Multidisciplinary Sciences
Thais Preisser Pontelo, Mauricio Machaim Franco, Taynan Stonoga Kawamoto, Felippe Manoel Costa Caixeta, Ligiane de Oliveira Leme, Nayara Ribeiro Kussano, Marcio Gilberto Zangeronimo, Margot Alves Nunes Dode
Summary: The presence of scriptaid during pre-maturation (PIVM) and/or maturation (IVM) did not improve the developmental competence or embryo quality of bovine oocytes, with lower blastocyst rates observed in embryos treated with scriptaid during PIVM and/or IVM.
Article
Cell Biology
Ju Hee Lee, Jae Kyun Park, Sook Young Yoon, Eun A. Park, Jin Hyun Jun, Hyunjung J. Lim, Jayeon Kim, Haengseok Song
Summary: Advanced maternal age (AMA) is associated with decreased quality and quantity of oocytes, making older oocytes more vulnerable to cryo-damage during vitrification. The cellular and molecular features of aged vitrified oocytes exhibit aberrations, leading to delayed embryo development. Collectively, current vitrification protocols optimized for young females may need to be modified for aged women.
Article
Reproductive Biology
F. Magata, A. Ideta, F. Matsuda, M. Urakawa, Y. Oono
Summary: The addition of GSH-OEt in oocyte development in aged cows can reduce oxidative damage, promote developmental competence, and increase the probability of fertilization and blastocyst formation, but has no significant effect on young cows.
Article
Agriculture, Dairy & Animal Science
Marwa El-Sheikh, Ahmed Atef Mesalam, Seon-Min Kang, Myeong-Don Joo, Seham Samir Soliman, Atif Ali Khan Khalil, Mi-Jeong Ahn, Il-Keun Kong
Summary: A study demonstrated that melatonin (MT) plays a protective role during in vitro embryo production (IVP) by reducing the deleterious effects of juglone, a polyphenolic extract of walnut trees, on bovine oocyte development. The study observed that melatonin supplementation regulated oocyte development, apoptosis, autophagy, and mitochondrial function, thereby protecting oocytes from oxidative stress.
Article
Genetics & Heredity
Ahmed Z. Balboula, Mansour Aboelenain, Miki Sakatani, Ken-Ichi Yamanaka, Hanako Bai, Takahiro Shirozu, Manabu Kawahara, Abd Elraouf O. Hegab, Samy M. Zaabel, Masashi Takahashi
Summary: Supplementation of E-64 significantly improves the developmental competence of OPU-derived immature oocytes and enhances the quality of blastocysts, suggesting its potential as a tool to enhance the efficiency of OPU-IVF program.
Article
Agriculture, Dairy & Animal Science
Juan P. Cabeza, Juan Camera, Olinda Briski, Minerva Yauri Felipe, Daniel F. Salamone, Andres Gambini
Summary: This study investigates the use of a novel zinc chelator for activating bovine and porcine oocytes. The results show that the zinc chelator can successfully induce blastocyst development in both species, although developmental rates and embryo quality are compromised in bovine compared to calcium-induced embryos. On the contrary, the new concentration and incubation time of the zinc chelator improve developmental rates in porcine without affecting embryo quality. These findings contribute to understanding the role of zinc in oocyte activation and preimplantation embryo development across different mammalian species.
Article
Developmental Biology
Shuta Nagata, Yuki Inoue, Takuya Sato, Keisuke Tanaka, Akihisa Shinozawa, Komei Shirasuna, Hisatala Iwata
Summary: This study demonstrates that ageing affects miRNA profiles in follicular fluid, and an miRNA called miR-19b, which is highly abundant in the follicular fluid of young cows, supports the growth of oocytes derived from early antral follicles.
Article
Reproductive Biology
Maria Florencia Suqueli Garcia, Nadia Gabbanelli, Glenda Laura Rios, Jorgelina Buschiazzo
Summary: This study found that progesterone (P4) can induce acrosome exocytosis in sperm without compromising sperm viability or activating sperm caspases. Furthermore, using lower concentrations of exogenous P4 during in vitro fertilization (IVF) can enhance the developmental competence of partially cumulus-denuded oocytes.
Article
Reproductive Biology
Amirhossein Abazarikia, Mahdi Zhandi, Armin Towhidi, Malak Shakeri, Ali Reza Youse, Amir Aliyan
Summary: The study showed that introducing conjugated linoleic acid (CLA) can improve oocyte developmental competence, reduce aberrant spindle organization of heat-stressed oocytes, and does not cause epigenetic alteration in the obtained blastocysts.
Review
Genetics & Heredity
Federica Innocenti, Giulia Fiorentino, Danilo Cimadomo, Daria Soscia, Silvia Garagna, Laura Rienzi, Filippo Maria Ubaldi, Maurizio Zuccotti
Summary: This mini review summarizes the crucial roles played by maternally inherited factors during oocyte maturation, fertilization, and preimplantation development, with a direct or indirect effect on the acquisition or maintenance of oocyte competence.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2022)
Article
Endocrinology & Metabolism
Qiyu Yang, Lixia Zhu, Meng Wang, Bo Huang, Zhou Li, Juan Hu, Qingsong Xi, Jing Liu, Lei Jin
Summary: This retrospective study observed the IVM process of 157 GV oocytes from 59 infertile couples and found that the duration of GV-MI of oocytes and the serum progesterone levels of patients significantly affected the developmental potential of the IVM oocytes. It is crucial to fertilize oocytes at the optimal maturation stage during in vitro maturation to improve the formation rate of high-quality embryos.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
K. K. Forrest, V. V. Flores, S. C. Gurule, S. Soto-Navarro, C. B. Shuster, C. A. Gifford, J. A. Hernandez Gifford
Summary: This study found that oocytes matured from high LPS follicles had a higher percentage of abnormal cells compared to those matured from low LPS follicles. Additionally, follicular E2 concentrations tended to be higher in high LPS follicles. However, mRNA transcript abundance for aromatase and beta-catenin was similar between the two groups.
ANIMAL REPRODUCTION SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Xi Xu, Baigao Yang, Hang Zhang, Xiaoyi Feng, Haisheng Hao, Weihua Du, Huabin Zhu, Adnan Khan, Muhammad Zahoor Khan, Peipei Zhang, Xueming Zhao
Summary: This study found that the use of 1 μM NMN can effectively reduce the lipid droplet content in bovine oocytes and decrease the expression levels of genes related to lipid synthesis. Vitrified bovine oocytes treated with 1 μM NMN had a higher survival rate and better development ability compared to other treatment groups. Additionally, 1 μM NMN, 2.5 μM BER, and 1 μM COR also decreased the levels of ROS and apoptosis, reduced the mRNA expression levels of genes involved in ER stress and mitochondrial fission, and increased the mRNA expression levels of genes associated with mitochondrial fusion in vitrified bovine oocytes. The results suggest that 1 μM NMN, 2.5 μM BER, and 1 μM COR effectively reduce lipid droplet content and enhance the development ability of vitrified bovine oocytes by regulating ROS levels, ER stress, mitochondrial function, and apoptosis. Furthermore, 1 μM NMN was more effective than 2.5 μM BER and 1 μM COR.
Article
Endocrinology & Metabolism
Desmond A. R. Tutt, Gizem Guven-Ates, Wing Yee Kwong, Rob Simmons, Fei Sang, Giuseppe Silvestri, Carla Canedo-Ribeiro, Alan H. Handyside, Remi Labrecque, Marc-Andre Sirard, Richard D. Emes, Darren K. Griffin, Kevin D. Sinclair
Summary: Removing complex proteins during bovine IVM affects the metabolism of cumulus cells. The addition of melatonin is beneficial in the presence of complex proteins but detrimental in their absence. These findings lay the foundation for developing chemically defined IVM systems for the generation of cytogenetically and epigenetically normal embryos.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Koki Nishihara, Daichi Kato, Yutaka Suzuki, Dahye Kim, Misato Nakano, Yu Yajima, Satoshi Haga, Miwa Nakano, Hiroshi Ishizaki, Ryouka Kawahara-Miki, Tomohiro Kono, Kazuo Katoh, Sang-gun Roh
JOURNAL OF ANIMAL SCIENCE
(2018)
Article
Multidisciplinary Sciences
Akiko Matsuura, Tamaki Ishima, Yuko Fujita, Yoshimi Iwayama, Shunsuke Hasegawa, Ryouka Kawahara-Miki, Motoko Maekawa, Manabu Toyoshima, Yusuke Ushida, Hiroyuki Suganuma, Satoshi Kida, Takeo Yoshikawa, Masaomi Iyo, Kenji Hashimoto
SCIENTIFIC REPORTS
(2018)
Article
Biochemistry & Molecular Biology
Kotomi Seno, Yasuhisa Munakata, Michiya Sano, Ryouka Kawahara-Miki, Hironori Takahashi, Akihide Ohkuchi, Hisataka Iwata, Takehito Kuwayama, Koumei Shirasuna
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Meeting Abstract
Immunology
Michiya Sano, Kotomi Seno, Yasuhisa Munakata, Ryouka Kawahara-Miki, Hironori Takahashi, Hisataka Iwata, Takehito Kuwayama, Akihide Ohkuchi, Koumei Shirasuna
JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2018)
Article
Biology
Ryo Futahashi, Yumi Yamahama, Migaku Kawaguchi, Naoki Mori, Daisuke Ishii, Genta Okude, Yuji Hirai, Ryouka Kawahara-Miki, Kazutoshi Yoshitake, Shunsuke Yajima, Takahiko Hariyama, Takema Fukatsu
Article
Biotechnology & Applied Microbiology
Manabu Nemoto, Hidekazu Niwa, Satoshi Murakami, Ryoka Miki, Tohru Higuchi, Hiroshi Bannai, Koji Tsujimura, Hiroshi Kokado
JOURNAL OF GENERAL VIROLOGY
(2019)
Article
Multidisciplinary Sciences
Shunsuke Hasegawa, Hotaka Fukushima, Hiroshi Hosoda, Tatsurou Serita, Rie Ishikawa, Tomohiro Rokukawa, Ryouka Kawahara-Miki, Yue Zhang, Miho Ohta, Shintaro Okada, Toshiyuki Tanimizu, Sheena A. Josselyn, Paul W. Frankland, Satoshi Kida
NATURE COMMUNICATIONS
(2019)
Article
Biochemistry & Molecular Biology
Hiroki Akizawa, Shun Saito, Nanami Kohri, Eri Furukawa, Yoshihiro Hayashi, Hanako Bai, Masashi Nagano, Yojiro Yanagawa, Hayato Tsukahara, Masashi Takahashi, Shinjiro Kagawa, Ryouka Kawahara-Miki, Hisato Kobayashi, Tomohiro Kono, Manabu Kawahara
Summary: This study dissected the molecular details of blastocyst stage in cattle using an optimized in vitro culture method, revealing dynamic expressions of key markers in TE cells over time. RNA-seq and qPCR analyses showed that TE cells highly expressed Wnt, FGF, and VEGF signaling pathways-related genes during their functional maturation.
Article
Agriculture, Dairy & Animal Science
Ryo Inaba, Ryouka Kawahara-Miki, Akihisa Shinozawa, Taichi Yasuhara, Takashi Fujii, Keisuke Koyama, Michiko Murata-Okubo, Kousaku Souma, Hiroki Hirayama
Summary: Hormonal induction of parturition in cattle increases the risk of retained fetal membrane. This study revealed the importance of interferon signaling in the immune regulation of placental tissues during parturition.
JOURNAL OF REPRODUCTION AND DEVELOPMENT
(2022)
Article
Multidisciplinary Sciences
Tappei Mishina, Hirohiko Takeshima, Mikumi Takada, Kei'ichiro Iguchi, Chunguang Zhang, Yahui Zhao, Ryouka Kawahara-Miki, Yasuyuki Hashiguchi, Ryoichi Tabata, Takeshi Sasaki, Mutsumi Nishida, Katsutoshi Watanabe
Summary: Research into the genetic diversity of triploid asexual Carassius auratus fish in Japan reveals a mixed genetic composition with alleles from both Japanese and Eurasian lineages. Interestingly, there is a unidirectional gene flow from sexual diploid relatives to sympatric triploids, contributing to the genetic similarity between the two groups. Additionally, the weaker population structure and multiple triploid lineages coexisting in Japanese rivers suggest that interploidy gene flow and increased establishment of immigrants may play a role in the successful distribution of this asexual vertebrate.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Genta Okude, Minoru Moriyama, Ryouka Kawahara-Miki, Shunsuke Yajima, Takema Fukatsu, Ryo Futahashi
Summary: The functional changes and interactions of transcription factors Kr-h1, broad, and E93 are crucial in the metamorphosis of insects. These findings are significant for understanding the evolution of insect metamorphosis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Mari Kawaguchi, Wen-Shan Chang, Hazuki Tsuchiya, Nana Kinoshita, Akira Miyaji, Ryouka Kawahara-Miki, Kenji Tomita, Atsushi Sogabe, Makiko Yorifuji, Tomohiro Kono, Toyoji Kaneko, Shigeki Yasumasu
Summary: The seahorse is a unique teleost fish with special morphology, including bony plates and spines, and males possessing a brooding organ called the brood pouch. The flame cone cells, found in the brood pouch and spines, express a gene called pgrich which is not present in related species. This pgrich gene may have originated from the elastin gene with the involvement of transposable elements and obtained its novel function in the flame cone cells during seahorse evolution.
CELL AND TISSUE RESEARCH
(2023)
Article
Multidisciplinary Sciences
Shu Hashimoto, Masaya Yamanaka, Takayuki Yamochi, Hisataka Iwata, Ryouka Kawahara-Miki, Masayasu Inoue, Yoshiharu Morimoto
SCIENTIFIC REPORTS
(2019)
Article
Multidisciplinary Sciences
Kazuo Yamagata, Kouhei Nagai, Hiroshi Miyamoto, Masayuki Anzai, Hiromi Kato, Kei Miyamoto, Satoshi Kurosaka, Rika Azuma, Igor I. Kolodeznikov, Albert Protopopov, Valerii V. Plotnikov, Hisato Kobayashi, Ryouka Kawahara-Miki, Tomohiro Kono, Masao Uchida, Yasuyuki Shibata, Tetsuya Handa, Hiroshi Kimura, Yoshihiko Hosoi, Tasuku Mitani, Kazuya Matsumoto, Akira Iritani
SCIENTIFIC REPORTS
(2019)
Article
Entomology
Genta Okude, Ryo Futahashi, Ryouka Kawahara-Miki, Kazutoshi Yoshitake, Shunsuke Yajima, Takema Fukatsu
APPLIED ENTOMOLOGY AND ZOOLOGY
(2017)