Article
Biology
Hiroki Akizawa, Emily M. Lopes, Rafael A. Fissore
Summary: The concentration of zinc ion in mouse eggs plays a crucial role in calcium ion responses and IP(3)R1 function during fertilization and egg activation, ensuring optimal response to fertilization and egg activation.
Article
Multidisciplinary Sciences
Takuya Kobayashi, Akihisa Tsutsumi, Nagomi Kurebayashi, Kei Saito, Masami Kodama, Takashi Sakurai, Masahide Kikkawa, Takashi Murayama, Haruo Ogawa
Summary: This study reveals the gating mechanism of RyR2 and how it is altered by pathogenic mutations through high-resolution structures and functional analysis.
NATURE COMMUNICATIONS
(2022)
Article
Pharmacology & Pharmacy
Anna Mosshammer, Lifang Zou, Stefan Boehm, Klaus Schicker
Summary: Many drugs used in cardiovascular therapy may exert their effects through the sympathetic nervous system. The P2Y(6) receptor has been identified as a new target for cardiovascular drugs, and its activation leads to sympathetic excitatory actions involving Ca2+-activated Cl- channels.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Juan Carlos Arevalo, Ruben Deogracias
Summary: Brain-derived nerve factor (BDNF), through TrkB receptor activation, modulates many physiological and pathological functions in the nervous system. It is crucial for brain circuit development and maintenance, synaptic plasticity, and neurodegenerative diseases. Regulation of BDNF concentrations at transcriptional, translational, and secretion levels is essential for proper central nervous system function. This review summarizes recent advances in understanding the molecular players involved in BDNF release and discusses how changes in these proteins impact BDNF-mediated functions in physiological and pathological conditions.
Article
Materials Science, Multidisciplinary
Qin Chen, Congyan Liu, Qing Gu, Ding Qu, Xinmeng Shi, Jingfa Zhang, Jun Sun, Huaijin Guan, Yan Chen, Xingwei Wu
Summary: Neovascular age-related macular degeneration (nAMD) is a leading cause of blindness worldwide due to abnormal angiogenesis. Current treatment using intraocular anti-VEGF agents is not effective for all patients and can cause severe complications. This study developed a novel injectable thermosensitive polymeric hydrogel loaded with tetramethylpyrazine (TMP) nanoparticles for sustained release and targeted therapy of choroidal neovascularization (CNV). The hydrogel-encapsulated TMP nanocrystals showed promising results in improving abnormal angiogenesis and preventing photoreceptor apoptosis without toxicity. This advanced formulation may represent an effective therapeutic strategy for patients with CNV.
APPLIED MATERIALS TODAY
(2023)
Article
Biochemistry & Molecular Biology
Iyuki Namekata, Kento Jitsukata, Ayumi Fukuda, Ryosuke Odaka, Shogo Hamaguchi, Hikaru Tanaka
Summary: Intracellular Ca2+-mediated mechanisms are involved in the pacemaker depolarization in both mouse and guinea pig sinus node tissues, but the Na+-Ca2+ exchanger (NCX) current is only involved in mice.
Article
Pharmacology & Pharmacy
Ritu Dwivedi, Bernard T. Drumm, Tuleen Alkawadri, S. Lorraine Martin, Gerard P. Sergeant, Mark A. Hollywood, Keith D. Thornbury
Summary: We investigated the effects of TMEM16A blockers on isometric contractions in mouse bronchial rings and intracellular calcium in bronchial myocytes. We found that benzbromarone and MONNA caused intracellular calcium release, resulting in a reduction of carbachol-induced contractions.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Developmental Biology
Anna Storey, Khalil Elgmati, Yisu Wang, Paul Knaggs, Karl Swann
Summary: The study revealed that mouse eggs are about 10 times more sensitive to InsP(3) than human eggs, and have consistently higher ATP levels. As a result, mouse eggs can undergo Ca2+ oscillations in response to Sr2+ stimulation, while human eggs may be unresponsive due to lower cytosolic ATP levels.
MOLECULAR HUMAN REPRODUCTION
(2021)
Article
Neurosciences
Grant F. Kusick, Tyler H. Ogunmowo, Shigeki Watanabe
Summary: Traditionally, it was believed that synaptic vesicles remain stably docked once they enter docking sites, awaiting calcium signals. However, recent studies have shown that vesicles can rapidly and reversibly transit between docking and undocking during activity. This finding accounts for many synaptic physiological phenomena.
CURRENT OPINION IN NEUROBIOLOGY
(2022)
Article
Neurosciences
Xianwei Zhang, Charlotte E. R. Smith, Stefano Morotti, Andrew G. Edwards, Daisuke Sato, William E. Louch, Haibo Ni, Eleonora Grandi
Summary: This study investigates the independent and interactive effects of TATS and Ca2+-handling protein remodelling on Ca2+-handling abnormality driven membrane instabilities. The results show that the impact of protein remodelling on Ca2+-driven proarrhythmic behaviour varies depending on TATS density.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Article
Biochemistry & Molecular Biology
Yonghao Xiao, Hui Zhao, Xiaoyu Ma, Zongheng Gu, Xin Wu, Liang Zhao, Lin Ye, Zengguo Feng
Summary: Due to the decreasing self-repairing ability, elder people are more prone to chronic wounds and slow wound healing. This study developed a hydrogel dressing by dissolving Heparin and basic fibroblast growth factor(bFGF) in a mixing solution, which can continuously release bFGF and exhibit better wound healing effect in elderly subjects.
Article
Multidisciplinary Sciences
Sheau-Chung Tang, Jiunn-Liang Ko, Chun-Te Lu, Pui-Ying Leong, Chu-Chyn Ou, Chih-Ting Hsu, Yu-Ping Hsiao
Summary: This study examined the pathway of cell death caused by skin burns in human skin cells and the role of chloroquine in wound healing. The findings suggest that autophagy and the Wnt/β-catenin pathway play important roles in cell repair and wound healing. Chloroquine can promote wound healing and restore cell migration activity.
Article
Biochemistry & Molecular Biology
Shogo Hamaguchi, Ikue Morinou, Yuko Shiseki, Ayako Mikami, Maika Seki, Iyuki Namekata, Hikaru Tanaka
Summary: Mechanisms of alpha-adrenoceptor-mediated positive inotropy were investigated in neonatal mouse ventricular myocardium. The positive inotropy induced by phenylephrine was suppressed by prazosin, nifedipine, and chelerythrine, but not by SEA0400. Phenylephrine increased L-type Ca2+ channel current and prolonged action potential duration, while voltage-dependent K+ channel current was unaffected. In the presence of cromakalim, the phenylephrine-induced prolongation of action potential duration and positive inotropy were smaller than in its absence, suggesting the involvement of ATP-sensitive K+ channels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Kouya Uchino, Hiroyuki Kawano, Yasuyoshi Tanaka, Yuna Adaniya, Ai Asahara, Masanobu Deshimaru, Kaori Kubota, Takuya Watanabe, Shutaro Katsurabayashi, Katsunori Iwasaki, Shinichi Hirose
Summary: Patients with Dravet syndrome exhibit abnormalities in inhibitory neurotransmission, which may exacerbate epileptic symptoms.
SCIENTIFIC REPORTS
(2021)
Article
Pharmacology & Pharmacy
Fenglian Zhang, Hanxing Wan, Fenglan Chu, Cheng Lu, Jun Chen, Hui Dong
Summary: This study found that glucose absorption through Na+-glucose co-transporter 1 in mouse upper jejunal epithelium is more efficient in the presence of extracellular Ca2+. Regulatory mechanisms involving Ca(v)1.3 channels, ER Ca2+ release, SOCE, and NCX1 were critical in modulating intestinal glucose uptake via Ca2+ signaling. Targeting these mechanisms in IEC may help regulate blood glucose and sodium levels in metabolic diseases.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)