Evolution of the Acquisition of Fertilization Competence and Polyspermy Blocks During Meiotic Maturation

In many animals, fully grown oocytes are arrested at prophase of meiosis I. Before or after ovulation/spawning, a secondary arrest occurs at metaphase of meiosis I or II (MI or II, respectively). MI arrest in the ovary is released after spawning, and is followed by fertilization, whereas MI and MII arrest after ovulation are released by fertilization. Insemination of isolated oocytes from the ovaries at an inappropriate time increases the rate of polyspermy, indicating that ovaries provide the proper environment for acquisition of the polyspermy blocks and the development of competence to be fertilized normally. Due to MI arrest in the ovaries or MI/MII arrest after ovulation/spawning, the fertilizable period can be elongated. Thus, MI and MII arrest may play a role in maintaining the cell-cycle phases to enable normal fertilization. Here, the evolution of fertilization timing is discussed.