Article
Reproductive Biology
Ewa Mlyczynska, Patrycja Kurowska, Dominika Wachowska, Malgorzata Grzesiak, Joelle Dupont, Agnieszka Rak
Summary: This study investigated the expression of phoenixin-14 in porcine corpus luteum and its impact on endocrine function. The results showed that phoenixin-14 can regulate the secretion of progesterone and estradiol and affect the expression of hormone receptors in luteal cells. Moreover, G protein-coupled receptor 173 and extracellular signal-regulated kinase 1/2 play important roles in the regulation of endocrine function in luteal cells.
BIOLOGY OF REPRODUCTION
(2023)
Article
Agriculture, Dairy & Animal Science
Ewa Mlyczynska, Ewa Zaobidna, Edyta Rytelewska, Kamil Dobrzy, Grzegorz Kopij, Karolina Szymanska, Patrycja Kurowska, Cecylia Dall'Aglio, Nina Smolinska, Tadeusz Kaminski, Agnieszka Rak
Summary: Visfatin/NAMPT is a hormone that connects energy metabolism with female reproduction. This study investigates the expression and localization of visfatin in the corpus luteum (CL) and its regulation by hormones such as LH, insulin, progesterone, prostaglandin E2, and F2 alpha. The study finds that visfatin expression in the porcine CL depends on the hormonal status of the estrous cycle or early pregnancy and is regulated by LH, insulin, progesterone, and prostaglandins through the ERK1/2 pathway.
ANIMAL REPRODUCTION SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Piotr Kaczynski, Monika Baryla, Ewelina Goryszewska, Agnieszka Waclawik
Summary: Intrauterine infusions of E2 can alter the expression of genes involved in CL function, potentially regulating DNA methylation as a physiological mechanism controlling luteal gene expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Karol Mierzejewski, Aleksandra Kurzynska, Zuzanna Gerwel, Monika Golubska, Robert Stryinski, Iwona Bogacka
Summary: This study aimed to investigate the in vitro effects of peroxisome proliferator-activated receptor-beta/delta (PPAR beta/delta) ligands on the transcriptomic profile of the lipopolysaccharide (LPS)-stimulated pig corpus luteum (CL) in the mid-luteal phase of the estrous cycle using RNA-seq technology. The results revealed that PPAR beta/delta agonists regulated the expression of genes involved in the inflammatory response in a dose-dependent manner. Lower dose of GW0724 showed an anti-inflammatory character, while higher dose seemed to be pro-inflammatory.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Reproductive Biology
Tomomi Kanazawa, Motohide Seki, Kosuke Iga
Summary: The present study aimed to evaluate the applicability of corpus luteum (CL) morphology and blood flow for early pregnancy diagnosis in Japanese Black (JB) cattle. The results showed that CL area and CL tissue area were greater in the pregnant group compared to the non-pregnant group on and after Day 19. Blood flow area (BFA) was also greater in the pregnant group on and after Day 18. Logistic regression analysis revealed that BFA on Days 17-20 was the best single predictor for pregnancy diagnosis, while CL area or CL tissue area on Day 21 was the most accurate predictor. BFA and central cavity area (CCA) were identified as effective predictors for early pregnancy diagnosis on Days 18-20.
Article
Agriculture, Dairy & Animal Science
Jamie M. Studer, Zoe E. Kiefer, Brady M. Goetz, Aileen F. Keating, Lance H. Baumgard, Zachary J. Rambo, Wesley P. Schweer, Mark E. Wilson, Christof Rapp, Jason W. Ross
Summary: The study investigated how a more bioavailable dietary Mn source can influence luteal function by altering the abundance of protein and mRNA involved in steroidogenesis. It was found that gilts fed Mn-amino acid complex had downregulated proteins related to P-4 signaling and cholesterol synthesis in the CL, and decreased transcript abundance of genes encoding steroidogenic enzymes in the CL tissue compared to controls.
JOURNAL OF ANIMAL SCIENCE
(2021)
Article
Food Science & Technology
Dainan Yu, Xun Jiang, Wenjing Ge, Bo Qiao, Dalei Zhang, Hui Liu, Haibin Kuang
Summary: This study found that acrylamide exposure during early- and mid-pregnancy had negative effects on luteal function, including decreased levels of serum progesterone and estradiol, reduced numbers and relative areas of ovarian corpora lutea, dysregulation of ovarian angiogenesis, increased oxidative stress, and induced apoptosis of ovarian cells.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Review
Agriculture, Dairy & Animal Science
J. L. Pate, C. H. K. Hughes
Summary: The corpus luteum plays a critical role in pregnancy in all mammals. However, the fate of the corpus luteum in ruminants depends on the presence of a functional uterus or signals from a developing embryo. Prostaglandin F2alpha, secreted by the uterus, is a key molecule that needs to be modified. The sensitivity of the corpus luteum to prostaglandin F2alpha can be influenced by internal mechanisms. Understanding the molecular changes that occur during different stages of luteal development and pregnancy can provide insight into the regulation of luteolytic capacity in ruminants.
Article
Reproductive Biology
M. Florencia Gallelli, Carolina Bianchi, Enzo Zampini, Mariana Bertuzzi, Marcelo Aba, Deborah Neild, Marcelo Miragaya
Summary: The administration of hCG on Day 7 post-mating in llamas was found to be effective in developing an accessory corpus luteum (CL) and increasing plasma progesterone (P4) concentration. This treatment had no impact on the original corpus luteum in pregnant llamas, but resulted in a larger corpus luteum diameter in treated non-pregnant llamas. The accessory CL was detected throughout the study in both pregnant and non-pregnant llamas, but its vascularization started to decrease around Day 16 post-mating in non-pregnant animals.
Review
Medicine, General & Internal
Zukaa Almahloul, Bedayah Amro, Zuhdi Nagshabandi, Iman Alkiumi, Zeinabs Hakim, Arnaud Wattiez, Muna Tahlak, Philippe R. R. Koninckx
Summary: Ovarian pregnancy is a rare condition with no established pathophysiology, diagnosis, and treatment. A review of case reports published on PubMed revealed that 8% of ovarian pregnancies occur in women with blocked oviducts and 23% are located on the other side of the corpus luteum. Abdominal bleeding should raise suspicion of ovarian pregnancy due to non-specific symptoms. Surgical excision is the preferred treatment, but caution must be taken not to interrupt an associated intra-uterine pregnancy.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Agriculture, Dairy & Animal Science
Jamie M. Studer, Zoe E. Kiefer, Brady M. Goetz, Aileen F. Keating, Lance H. Baumgard, Zachary J. Rambo, Wesley P. Schweer, Mark E. Wilson, Christof Rapp, Jason W. Ross
Summary: The study suggests that feeding a more bioavailable source of manganese can increase manganese accumulation in corpora lutea tissue and influence corpora lutea function.
JOURNAL OF ANIMAL SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Adomar Laurindo Neto, Felipe Lopes Ruas, Bruno Silva Espirito Santo, Ana Clara Degan Mattos, Junior Borges Silva, Marcos Flavio Diniz Jr, Danilo Fonseca Cardoso Pereira, Izabelle Pereira Lacerda, Jose Oliveira Carvalho, Guilherme Pugliesi
Summary: This study tested the impact of supplementing long-acting P4 at different times during the initial dioestrus on the pregnancy rates of dairy and beef recipients undergoing fixed-time embryo transfer. The results showed that using iP4 during FTET can enhance the pregnancy rates of beef recipients, although the effectiveness of iP4 supplementation on pregnancy maintenance may vary under different experimental conditions.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Agriculture, Dairy & Animal Science
Kinga Orzechowska, Marta Kiezun, Edyta Rytelewska, Marlena Gudelska, Katarzyna Kisielewska, Grzegorz Kopij, Kamil Dobrzyn, Ewa Zaobidna, Karolina Szymanska, Tadeusz Kaminski, Nina Smolinska
Summary: This study aimed to evaluate the effects of orexin A (OXA) on mRNA abundance of enzymes involved in prostaglandin production and the concentrations of prostaglandin E2 (PGE2) and F-2 alpha (PGF(2 alpha)) in endometrial and myometrial explants. The results suggest that OXA may affect the synthesis and secretion of PGE2 and PGF(2 alpha) in the uterus by regulating the mRNA levels of enzymes involved in prostaglandin production.
ANIMAL REPRODUCTION SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Bartlomiej Maria Jaskowski, Hartwig Bostedt, Marek Gehrke, Jedrzej Maria Jaskowski
Summary: The study shows that recipients with a cavitary corpus luteum have higher progesterone levels and pregnancy rates during embryo transfer. Therefore, the presence of a cavity inside the corpus luteum should not be a reason to exclude recipients from embryo transfer.
Review
Veterinary Sciences
Fang Chen, Yi'an Hou, Xiaoqing Zhu, Cheng Mei, Rihong Guo, Zhendan Shi
Summary: In recent decades, embryo transfer has been widely used in dairy cows, but improving the conception rate after transfer remains a major challenge. This review conducted a meta-analysis to evaluate the efficacy of hCG and GnRH in improving pregnancy rates after transfer. The results showed that hCG and GnRH can improve pregnancy rates in cows with poor fertility, but not in cows with good fertility.
VETERINARY SCIENCES
(2023)
Article
Cell Biology
Joanna Bukowska, Katarzyna Walendzik, Marta Kopcewicz, Patrycja Cierniak, Barbara Gawronska-Kozak
Summary: The study investigates the role of Wnt signaling in cutaneous wound repair, showing that Wnt ligands contribute to skin wound healing primarily by engaging in extracellular matrix maturation. The data suggests a potential relationship between Foxn1 and Wnts in post-traumatic skin tissue.
CONNECTIVE TISSUE RESEARCH
(2021)
Article
Reproductive Biology
Ewelina Goryszewska, Piotr Kaczynski, Monika Baryla, Agnieszka Waclawik
Summary: Our study suggests that prokineticin 1 acts as a pleiotropic factor by regulating angiogenesis, trophoblast adhesion and invasion, immunological response, and cell proliferation in the porcine endometrium through the PROKR1 signaling pathway.
BIOLOGY OF REPRODUCTION
(2021)
Article
Reproductive Biology
Piotr Kaczynski, Stefan Bauersachs, Ewelina Goryszewska, Monika Baryla, Agnieszka Waclawik
Summary: In vivo study revealed that prostaglandin E2 acting simultaneously with estradiol-17 beta induces more changes within porcine endometrial transcriptome resembling pregnancy effects compared with estradiol-17 beta administered alone.
BIOLOGY OF REPRODUCTION
(2021)
Article
Biochemistry & Molecular Biology
Sylwia Machcinska, Marta Kopcewicz, Joanna Bukowska, Katarzyna Walendzik, Barbara Gawronska-Kozak
Summary: The study found that interaction between Foxn1 and hypoxia-regulated factors affects re-epithelialization and growth factor expression in scarless skin wound healing in mice.
Article
Biochemistry & Molecular Biology
Piotr Kaczynski, Monika Baryla, Ewelina Goryszewska, Agnieszka Waclawik
Summary: Intrauterine infusions of E2 can alter the expression of genes involved in CL function, potentially regulating DNA methylation as a physiological mechanism controlling luteal gene expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Mari van de Vyver, Kiara Boodhoo, Trivia Frazier, Katie Hamel, Marta Kopcewicz, Benjamin Levi, Michelle Maartens, Sylwia Machcinska, Johanna Nunez, Chase Pagani, Emma Rogers, Katarzyna Walendzik, Joanna Wisniewska, Barbara Gawronska-Kozak, Jeffrey M. Gimble
Summary: This study aims to develop and validate a scoring system based on healing parameters in each phase, which can be easily and accurately assessed using H&E or MT staining. The study compares parameters assessed using different staining methods and reports high sensitivity for each parameter, ranging between 80% and 95%. The validated scoring system defines minimum criteria for assessing wound healing dynamics and is associated with academic and scientific institutions involved in its development.
STEM CELLS AND DEVELOPMENT
(2021)
Article
Multidisciplinary Sciences
Ewelina Goryszewska-Szczurek, Monika Baryla, Piotr Kaczynski, Agnieszka Waclawik
Summary: Prokineticin 1 plays a pleiotropic role in trophoblast function during implantation and early placentation, with its signaling pathway regulating key functions such as cell proliferation and adhesion. The study also identified the involvement of various signaling pathways, including MAPK, PI3K/AKT/mTOR, and cAMP, in mediating the effects of PROK1 in trophoblasts. The findings suggest that PROK1 has a significant impact on trophoblast development and function in the early stages of pregnancy.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Barbara Gawronska-Kozak, Katarzyna Walendzik, Sylwia Machcinska, Artur Padzik, Marta Kopcewicz, Joanna Wisniewska
Summary: This study demonstrates for the first time that Foxn1 and Hif-1 alpha cooperatively regulate dWAT during skin wound healing, promoting adipocyte generation through the Igf2 signaling pathway, while reducing macrophage content in the wound site.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Piotr Kaczynski, Ewelina Goryszewska-Szczurek, Monika Baryla, Agnieszka Waclawik
Summary: The establishment of pregnancy in mammals, including pigs, requires communication between developing embryos and the maternal organism. Pigs signalize their presence by secreting factors such as estradiol-17 beta (E2), initiating the maternal recognition of pregnancy. Prostaglandin (PGF2 alpha) and luteoprotective factors contribute to the maintenance of progesterone secretion, preparing the uterus for implantation. Embryo implantation involves a proinflammatory environment coordinated by the secretion of cytokines, growth factors, and lipid mediators. Recent studies have identified novel factors and revealed complex relationships with established factors. Early pregnancy is a sequence of processes orchestrated by pleiotropic factors, preserving essential functions critical for implantation and placenta formation.
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2023)
Article
Biochemistry & Molecular Biology
Sylwia Machcinska, Katarzyna Walendzik, Marta Kopcewicz, Joanna Wisniewska, Anne Rokka, Mirva Paakkonen, Mariola Slowinska, Barbara Gawronska-Kozak
Summary: This study reveals that Foxn1 in keratinocytes plays a role in activating antioxidant defense and controlling functional properties, especially in response to hypoxic environment. It regulates elements of the electron transport chain and participates in the induction of the thioredoxin system, affecting keratinocyte migration and angiogenesis.
Article
Reproductive Biology
Piotr Kaczynski, Vera van der Weijden, Ewelina Goryszewska-Szczurek, Monika Baryla, Susanne E. Ulbrich, Agnieszka Waclawik
Summary: This study found that CpG methylation induced by embryonic signals may contribute to regulating endometrial gene expression during early pregnancy in pigs.
BIOLOGY OF REPRODUCTION
(2022)
Article
Oncology
Joanna Wis, Magda Slyszewska, Marta Kopcewicz, Katarzyna Walendzik, Sylwia Machcinska, Karolina Stalanowska, Barbara Gawronska-Kozak
Summary: This study evaluated the effects of pig adipose-derived stem cells (pASCs) cultured under different conditions on wound healing in a mouse model. The results showed that hypoxia-preconditioned pASCs accelerated wound healing, increased hyaluronic acid content, and reduced scar formation. Transplantation of hypoxia-preconditioned pASCs also promoted angiogenesis and decreased pro-scarring signaling pathways. However, at early stages of healing, wounds treated with hypoxia-preconditioned pASCs exhibited decreased elasticity and increased expression of scar-forming signaling pathways.
EXPERIMENTAL CELL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Katarzyna Walendzik, Marta Kopcewicz, Joanna Wisniewska, Paulina Opyd, Sylwia Machcinska-Zielinska, Barbara Gawronska-Kozak
Summary: The epidermally expressed Foxn1 gene regulates the development of dermal white adipose tissue (dWAT) and determines the ability of dermal fibroblasts to generate adipocytes. This study identifies the contribution of Foxn1 to dWAT metabolism and suggests potential targets for modulating and regulating dWAT in physiological and pathological processes in the skin.
Article
Multidisciplinary Sciences
Monika Baryla, Ewelina Goryszewska-Szczurek, Piotr Kaczynski, Gianfranco Balboni, Agnieszka Waclawik
Summary: This study investigated the role of Prokineticin 1 (PROK1) in the corpus luteum (CL) during pregnancy and found that PROK1 is essential for regulating steroidogenesis, luteal cell apoptosis and viability, and angiogenesis in maintaining luteal function.
SCIENTIFIC REPORTS
(2023)
Article
Cell & Tissue Engineering
Joanna Bukowska, Anna Zuzanna Szostek-Mioduchowska, Marta Kopcewicz, Katarzyna Walendzik, Sylwia Machcinska, Barbara Gawronska-Kozak
Summary: ASCs isolated from domestic animals have shown potential for preventing and treating human diseases, particularly in large animal models such as pigs and horses. Current research indicates that ASC-based therapies have significant benefits in promoting animal health and have promising prospects for future applications.
STEM CELL REVIEWS AND REPORTS
(2021)
