Article
Multidisciplinary Sciences
Hemant Chandru Naik, Kishore Hari, Deepshikha Chandel, Susmita Mandal, Mohit Kumar Jolly, Srimonta Gayen
Summary: Recent studies have shown the widespread presence of dynamic random monoallelic expression of autosomal genes during pregastrulation. The coordination of allelic bursting is lineage specific, with genes regulating development showing higher levels of coordination. This provides significant insights into the prevalence and origin of dynamic aRME during early development.
Article
Multidisciplinary Sciences
Ryo Maekawa, Shun Sato, Tetsuro Tamehisa, Takahiro Sakai, Takuya Kajimura, Kotaro Sueoka, Norihiro Sugino
Summary: This study compared the DNA methylome, transcriptome, and histological features between MED12m-positive and -negative uterine fibroids (UFs). It was found that MED12m-positive UFs have increased extracellular matrix formation activity, while MED12m-negative UFs have increased angiogenic activity and smooth muscle cell proliferation.
SCIENTIFIC REPORTS
(2022)
Article
Psychology, Multidisciplinary
Judith Streb, Elena Ruppel, Anne-Maria Moeller-Leimkuehler, Michael Buesselmann, Irina Franke, Manuela Dudeck
Summary: Despite similar externalizing behaviors, a significant relationship between external and internal depression symptoms was found only in men in this forensic psychiatric sample. Male forensic patients with a history of suicide had higher scores in the Gender-Sensitive Depression Screening, while female patients with a history of suicide had higher scores in the Beck Depression Inventory Revision. These findings suggest that the Gender-Sensitive Depression Screening may be useful for detecting depression symptoms in men and developing suicide prevention assessments in forensic practice.
FRONTIERS IN PSYCHOLOGY
(2021)
Article
Clinical Neurology
Nikolaj Kjaer Hoier, Trine Madsen, Adam P. Spira, Keith Hawton, Michael Eriksen Benros, Merete Nordentoft, Annette Erlangsen
Summary: This study examined the association between sleep disorders and suicide rates in Denmark between 1980 and 2016. The results showed that both males and females diagnosed with sleep disorders had higher suicide rates compared to those without sleep disorders, even after adjusting for preexisting mental disorders.
Article
Multidisciplinary Sciences
Md. Sohan, M. M. A. Shalahuddin Qusar, Mohammad Shahriar, Sardar Mohammad Ashraful Islam, Mohiuddin Ahmed Bhuiyan, Md. Rabiul Islam
Summary: This study compared the serum levels of pro-inflammatory cytokine leptin and neurotrophic factor EGF in healthy controls and MDD patients. The findings showed reduced serum EGF levels in MDD patients, but no correlation was found between serum EGF levels and the severity of depression.
Article
Clinical Neurology
Chunyu Guo, Yingying Cui, Zhengmei Xia, Jie Hu, Yanni Xue, Xuexue Huang, Yuhui Wan, Jun Fang, Shichen Zhang
Summary: This study examines the relationships between health literacy, depressive symptoms, and suicide-related outcomes in adolescents. The findings suggest that adequate health literacy is associated with lower levels of depressive symptoms and suicide-related outcomes, particularly in female students.
JOURNAL OF AFFECTIVE DISORDERS
(2023)
Article
Biochemistry & Molecular Biology
Alexander Knyshov, Eric R. L. Gordon, Paul K. Masonick, Stephanie Castillo, Dimitri Forero, Rochelle Hoey-Chamberlain, Wei Song Hwang, Kevin P. Johnson, Alan R. Lemmon, Emily Moriarty Lemmon, Samantha Standring, Junxia Zhang, Christiane Weirauch
Summary: Despite the difference in phylogenetic signals between genes on sex chromosomes and autosomes, the conservation of chromosomal gene content across deep phylogenetic scales in nonvertebrates has not been extensively studied. In this research, we assembled a large and diverse set of samples to investigate the phylogeny of assassin bugs. Our results show that genes on the X chromosome have more discordant phylogenies than those on autosomes, driven by high gene substitution rates. We conclude that binning genes to autosomal or sex chromosomes can provide a more accurate understanding of the evolutionary history of a clade.
MOLECULAR BIOLOGY AND EVOLUTION
(2023)
Article
Oncology
Sayaka Kawashima, Atsushi Hattori, Erina Suzuki, Keiko Matsubara, Machiko Toki, Rika Kosaki, Yukihiro Hasegawa, Kazuhiko Nakabayashi, Maki Fukami, Masayo Kagami
Summary: Research indicates that X-chromosome rearrangements generally do not impact the methylation status of escape gene promoters, except in cases of highly complex rearrangements involving the deletion of the FIRRE gene and the duplication of the DXZ4 gene.
CLINICAL EPIGENETICS
(2021)
Article
Biochemistry & Molecular Biology
Shahar Shohat, Ethel Vol, Sagiv Shifman
Summary: Sex differences in humans are influenced by gonadal hormones and sex chromosomes. This study explores how sex chromosomes can affect gene expression and the outcome of mutations, using cancer cell lines as a model system. The findings highlight the roles of X and Y chromosomes in sex-specific cell function.
Article
Psychology, Developmental
Jing Yu, Rise B. Goldstein, Denise L. Haynie, Jeremy W. Luk, Brian J. Fairman, Reeya A. Patel, Pablo Vidal-Ribas, Katherine Maultsby, Mahad Gudal, Stephen E. Gilman
Summary: The study found that life satisfaction and optimism can reduce the risk of suicidal thoughts and behaviors among adolescents with depressive symptoms. Resilience factors such as life satisfaction and optimism may buffer against suicidality risk in the face of mild or moderate to severe depressive symptoms for adolescents transitioning to young adulthood.
JOURNAL OF ADOLESCENT HEALTH
(2021)
Article
Behavioral Sciences
Rabia Yilmaz, Didem Behice Oztop, Elif Funda Sener, Merve Cikili-Uytun, Fatma Dal, Emrah Yildiz, Musa Sahpolat, Gokmen Zararsiz
Summary: The study indicates that BDNF gene expression is more associated with psychiatric disorders rather than suicide attempt in children and adolescents. The suicide group showed significantly higher scores in problem-solving skills and childhood trauma experiences compared to the control group.
BEHAVIOURAL BRAIN RESEARCH
(2021)
Article
Health Care Sciences & Services
Aleksandra Skiba, Monika Talarowska, Janusz Szemraj, Piotr Galecki
Summary: This study aimed to investigate the role of the NRXN1 gene in the etiology and epigenetics of depression by comparing NRXN1 mRNA and protein expression levels in patients with depression and healthy controls. The results showed that NRXN1 gene expression was significantly lower in patients with depression compared to healthy individuals, and the expression levels varied with the severity of depression symptoms. These findings may contribute to further research on the pathogenesis of depressive disorders and personalized treatment approaches.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Multidisciplinary Sciences
Sara Ballouz, Risa Karakida Kawaguchi, Maria T. Pena, Stephan Fischer, Megan Crow, Leon French, Frank M. Knight, Linda B. Adams, Jesse Gillis
Summary: This study explores the stochastic variation in allele-specific expression during organism development. The research on nine-banded armadillos reveals the enduring signal of autosomal allelic variability and its influence on phenotype. The findings shed light on the non-genetic factors shaping organismal diversity and provide insights into the inconsistent penetrance associated with stochastic allelic expressions.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Qingqin S. Li, Randall L. Morrison, Gustavo Turecki, Wayne C. Drevets
Summary: Epigenetic mechanisms play a significant role in the etiology of major depressive disorder (MDD), as indicated by a meta-analysis study. The study identified differentially methylated positions (DMPs) and differentially methylated regions (DMRs) associated with MDD, highlighting the involvement of pathways related to neuronal synaptic plasticity, calcium signaling, and inflammation.
SCIENTIFIC REPORTS
(2022)
Article
Dermatology
L. Hefez, J. Micallef, A. Revah-Levy, B. Falissard, E. Jouve, B. Dreno, O. Chosidow
Summary: The use of Adolescent Depression Rating Scale (ADRS) in monitoring symptoms of adolescents receiving isotretinoin treatment in dermatology practice was found to be valuable and satisfactory for both dermatologists and patients.
CLINICAL AND EXPERIMENTAL DERMATOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Massimiliano Orri, Jean R. Seguin, Natalie Castellanos-Ryan, Richard E. Tremblay, Sylvana M. Cote, Gustavo Turecki, Marie-Claude Geoffroy
Summary: Evidence from a quasi-experimental study based on genetic data suggests a causal role of cannabis, alcohol, and tobacco smoking on suicide attempt, with cannabis showing a direct pathway to suicide attempt and the effects of alcohol and smoking possibly mediated by other substance use phenotypes. No evidence was found for reverse causation.
MOLECULAR PSYCHIATRY
(2021)
Article
Neurosciences
Pierre-Eric Lutz, Daniel Almeida, Dominique Filliol, Fabrice Jollant, Brigitte L. Kieffer, Gustavo Turecki
Summary: Research indicates that depression is associated with increased MOR activity, partially explained by a specific increase in expression of the inhibitory alpha G-protein subunit GNAI2. This enhanced endogenous opioidergic tone may help buffer negative affective states in depressed individuals.
NEUROPSYCHOPHARMACOLOGY
(2021)
Article
Psychiatry
Gerd Wagner, Meng Li, Matthew D. Sacchet, Stephane Richard-Devantoy, Gustavo Turecki, Karl-Jurgen Bar, Ian H. Gotlib, Martin Walter, Fabrice Jollant
Summary: This study investigates the neural correlates of suicidal ideation and suicidal behavior, suggesting that suicide attempters exhibit distinct brain alterations compared to suicide ideators, patient controls, and healthy controls. The findings indirectly support the relevance of the transition model at the neurobiological level and highlight the need for further research on the brain bases of suicidal ideas in depressed individuals.
TRANSLATIONAL PSYCHIATRY
(2021)
Article
Biochemistry & Molecular Biology
Ashutosh Tripathi, Carl Whitehead, Katelyn Surrao, Ananya Pillai, Amit Madeshiya, Yong Li, Hesam Khodadadi, Anthony O. Ahmed, Gustavo Turecki, Babak Baban, Anilkumar Pillai
Summary: This study demonstrated that chronic stress induces a significant increase in serum IFN beta levels in mice, and systemic blockade of IFN-I signaling alleviates stress-induced neuroinflammation and behavioral abnormalities. It also revealed the mediating role of C3 in these processes and found a significant increase in IFN-I stimulated genes in the prefrontal cortex of depressed suicide subjects with correlation to inflammatory markers.
MOLECULAR PSYCHIATRY
(2021)
Article
Biochemical Research Methods
Malosree Maitra, Corina Nagy, Anjali Chawla, Yu Chang Wang, Camila Nascimento, Matthew Suderman, Jean-Francois Theroux, Naguib Mechawar, Jiannis Ragoussis, Gustavo Turecki
Summary: The authors present an optimized workflow for isolating single nuclei from archived postmortem tissues, which does not require sorting or ultracentrifugation. This protocol allows for efficient capture of single nuclei for high-quality snRNA-seq libraries and is compatible with the 10X Genomics Chromium system.
Article
Genetics & Heredity
Dalileia A. Santana, Amina Bedrat, Renato D. Puga, Gustavo Turecki, Naguib Mechawar, Tathyane C. Faria, Carolina O. Gigek, Spencer L. M. Payao, Marilia A. C. Smith, Bernardo Lemos, Elizabeth S. Chen
Summary: This study aimed to evaluate H3K9 acetylation and gene expression profiles in three brain regions of Alzheimer's disease (AD) patients and controls, and to identify AD region-specific abnormalities. The results showed hyperacetylation in the cerebellum and slight hypoacetylation in the hippocampus of AD patients. Differentially expressed genes were found in the hippocampus and auditory cortex. Network analysis revealed Rho GTPase-mediated mechanisms. These findings suggest dysregulation of crucial mechanisms in AD patients at the epigenetic and transcriptomic levels, which could contribute to future research on AD pathogenesis.
Article
Genetics & Heredity
Lai Jiang, Keelin Greenlaw, Antonio Ciampi, Angelo J. Canty, Jeffrey Gross, Gustavo Turecki, Celia M. T. Greenwood
Summary: 5-hydroxymethylcytosine (5hmC) is a methylation state associated with gene regulation, commonly found in cells of the central nervous system. Existing methods for analyzing 5hmC data have limitations, and we propose a hierarchical Bayesian method called CHYME to simultaneously estimate 5mC/5hmC signals and their associations with covariates or phenotypes, while accounting for DNA damage and experimental design dependencies. Simulations show that CHYME has better statistical power than other methods and can reveal associations between 5mC/5hmC levels and phenotypes.
GENETIC EPIDEMIOLOGY
(2022)
Article
Psychology, Developmental
Lea C. Perret, Michel Boivin, Genevieve Morneau-Vaillancourt, Till F. M. Andlauer, Stephane Paquin, Stephanie Langevin, Alain Girard, Gustavo Turecki, Kieran O'Donnell, Richard E. Tremblay, Sylvana M. Cote, Jean-Philippe Gouin, Isabelle Ouellet-Morin, Marie-Claude Geoffroy
Summary: This study examined whether having a genetic predisposition to depression increased the risk of experiencing depressive symptoms in peer victimised youth. The results showed that both self- and teacher-reported peer victimisation were associated with depressive symptoms in adolescence, and this association remained significant when accounting for genetic predisposition. However, there was no significant interaction between genetic predisposition and peer victimisation. The findings suggest that genetic predisposition and exposure to peer victimisation are independently associated with depressive symptoms in adolescence.
JOURNAL OF CHILD PSYCHOLOGY AND PSYCHIATRY
(2023)
Article
Biochemistry & Molecular Biology
R. J. Scott Lacombe, Mackenzie E. Smith, Kelly Perlman, Gustavo Turecki, Naguib Mechawar, Richard P. Bazinet
Summary: Through analysis of human brain hemispheres, it was found that oleic acid was the most abundant fatty acid in the human brain, and cholesterol and saturated fatty acids were more enriched in C-13 isotope, while polyunsaturated fatty acids were more depleted in C-13 isotope. These findings provide new insights into the origin and concentration of fatty acids in the human brain.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
El Cherif Ibrahim, Victor Gorgievski, Pilar Ortiz-Teba, Raoul Belzeaux, Gustavo Turecki, Etienne Sibille, Guillaume Charbonnier, Eleni T. Tzavara
Summary: Antidepressants are currently the best treatment for moderate to severe major depressive episodes, but the trial-and-error prescription strategy and side effects are concerning. There is a lack of biomarkers to aid in treatment decisions, highlighting the need for further research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Psychiatry
Kitty Reemst, Laura Kracht, Janssen M. Kotah, Reza Rahimian, Astrid A. S. van Irsen, Gonzalo Congrains Sotomayor, Laura N. Verboon, Nieske Brouwer, Sophie Simard, Gustavo Turecki, Naguib Mechawar, Susanne M. Kooistra, Bart J. L. Eggen, Aniko Korosi
Summary: Early-life stress has persistent effects on microglial function and may contribute to the development of psychiatric disorders.
TRANSLATIONAL PSYCHIATRY
(2022)
Article
Cell Biology
Bruno Mari Fredi, Roger Willian De Labio, Lucas Trevizani Rasmussen, Eduardo Federighi Baisi Chagas, Elizabeth Suchi Chen, Gustavo Turecki, Marilia de Arruda Cardoso Smith, Spencer Luiz Marques Payao
Summary: Alzheimer's disease is a progressive neuroinflammatory and neurodegenerative disorder that affects different regions of the brain. The accumulation of beta-amyloid protein, formation of neurofibrillary tangles, and inflammatory processes contribute to its pathophysiology. CDK10 and CDK11 have high expression in AD patients compared to control, and they present a positive correlation of gene expression in the analyzed groups and tissues, suggesting their important role in the pathogenesis of AD.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Edwin J. C. G. van den Oord, Lin Y. Xie, Min Zhao, Thomas L. Campbell, Gustavo Turecki, Anna K. Kahler, Brian Dean, Ole Mors, Christina M. Hultman, Nicklas H. Staunstrup, Karolina A. Aberg
Summary: Schizophrenia is a disabling disorder with unclear pathophysiology, but a recent study using methylome-wide association analysis found suggestive associations in all cell types and whole blood, as well as significant associations in monocytes, natural killer cells, and B cells. Validation of these methylomic findings in post-mortem brains revealed significant transcriptional differences correlated with schizophrenia, providing potential genes and loci of functional relevance. This unique approach combining pre-disease onset blood data and post-disease onset brain data may lead to the development of methylation-based biomarkers for early detection of schizophrenia susceptibility.
MOLECULAR PSYCHIATRY
(2023)
Article
Neurosciences
Nicolas Garel, Kyle T. T. Greenway, Le-Anh L. Dinh-Williams, Julien Thibault-Levesque, Didier Jutras-Aswad, Gustavo Turecki, Soham Rej, Stephane Richard-Devantoy
Summary: We provide evidence that sub-anesthetic ketamine infusions can facilitate the discontinuation of long-term benzodiazepine/z-drugs for treatment-resistant depression. In this cohort study, 91% of patients successfully discontinued all benzodiazepine/z-drugs after receiving a course of ketamine infusions. Less than 25% of discontinuers experienced significant worsening of symptoms during the discontinuation phase. During follow-up, 64% of patients remained abstinent from any benzodiazepine/z-drugs. These preliminary results suggest that ketamine infusions may be a novel approach for deprescribing benzodiazepine/z-drugs in patients with treatment-resistant depression.
NEUROPSYCHOPHARMACOLOGY
(2023)
Article
Biochemical Research Methods
Alice Morgunova, Pascal Ibrahim, Gary Gang Chen, Sache M. Coury, Gustavo Turecki, Michael J. Meaney, Anthony Gifuni, Ian H. Gotlib, Corina Nagy, Tiffany C. Ho, Cecilia Flores
Summary: Dried blood spots (DBS) are a common method to collect biological samples, especially for newborns and in remote areas. MicroRNAs (miRNAs) play critical roles in diseases and are used as markers and regulators. This protocol provides optimized steps to obtain peripheral miRNA expression profiles from DBS, including key practices and the benefits of standardization.
BIOLOGY METHODS & PROTOCOLS
(2023)