Article
Biochemistry & Molecular Biology
Zhan Li, Ying Wang, Lili Yu, Yongzhe Gu, Lijuan Zhang, Jun Wang, Lijuan Qiu
Summary: The increasing problem of soil salinization has a severe impact on soybean growth and development, leading to reduced yields and posing a serious threat to global crop production. In this study, the PfFAD3a gene was isolated from perilla and overexpressed in soybeans to assess the salt tolerance of transgenic plants. The results showed that overexpression of PfFAD3a increased the expression of PfFAD3a and the content of ff-linolenic acid in transgenic soybean plants, significantly enhancing their salt tolerance. Physiological and biochemical analysis revealed that overexpression of PfFAD3a increased the chlorophyll content and maximum photochemical efficiency of transgenic soybean plants under salt stress, while reducing the accumulation of MDA, H2O2, and O-circle(2), and increasing the activities of SOD, POD, CAT, and APX, as well as the production of proline and soluble sugar. In conclusion, the overexpression of PfFAD3a enhances salt tolerance in transgenic soybean plants through improved membrane fluidity and increased antioxidant capacity induced by C18:3.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Xiaoyue Yu, Yu Pan, Yan Dong, Bin Lu, Chao Zhang, Minsheng Yang, Lihui Zuo
Summary: PeWRKY31 was isolated from Populus x euramericana, and overexpression of PeWRKY31 improved the salt tolerance and insect resistance of transgenic tobacco. The study provides references for the generation of stress-resistant lines with potentially great economic benefit.
Article
Plant Sciences
Peiqi Xian, Yuan Yang, Chuwen Xiong, Zhibin Guo, Intikhab Alam, Zihang He, Yakun Zhang, Zhandong Cai, Hai Nian
Summary: In this study, a Cd-responsive WRKY transcription factor GmWRKY172 was identified and found to enhance Cd tolerance and reduce Cd accumulation in soybean. Transgenic plants overexpressing GmWRKY172 showed better growth and lower Cd content under Cd stress. RNA sequencing analysis revealed that GmWRKY172 regulates multiple stress-related pathways to enhance Cd tolerance in soybean.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Wenqiang Wang, Yang Yang, Yanmei Deng, Zhigang Wang, Yuchao Yuan, Shenlin Yang, Juan Qi, Jiajie Wu, Daolin Fu, Wei Wang, Qunqun Hao
Summary: The study found that barley transgenic lines overexpressing ICS exhibit enhanced salt stress tolerance, attributed to improved Na+/K+ ratio, Na+ sequestration, and antioxidative competence under salt stress.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Agronomy
Zhanyu Chen, Xiaokun Fang, Xueshun Yuan, Yingying Zhang, Huiying Li, Ying Zhou, Xiyan Cui
Summary: The overexpression of transcription factor GmTGA15 in soybean and Arabidopsis plants enhances tolerance to drought stress by increasing chlorophyll and proline content, and decreasing malondialdehyde content. Transgenic Arabidopsis plants exhibit longer roots and lower mortality rate under simulated drought conditions.
Article
Biochemistry & Molecular Biology
Jiao Liu, Leru Liu, Lu Tian, Shaoming Xu, Guojiang Wu, Huawu Jiang, Yaping Chen
Summary: In this study, the expression characteristics and potential functions of the polyol transporter LjPLT3 in Lotus japonicus under salt stress were investigated. LjPLT3 was found to be expressed in the vascular tissue of leaves, stems, roots, and nodules, and its expression was induced by NaCl treatment. Overexpression of LjPLT3 in L. japonicus resulted in modified growth rate and saline tolerance of the transgenic plants. Transgenic seedlings exhibited higher chlorophyll concentration, fresh weight, and survival rate after salt treatment compared to the wild type.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Zhuo Zhang, Yuanling Zhao, Yifan Chen, Yueming Li, Lijun Pan, Siyu Wang, Piwu Wang, Sujie Fan
Summary: In this study, a TCP transcription factor, TCP9-like, was identified in salt-tolerant soybean cultivars and its overexpression enhanced salt tolerance by regulating the accumulation of Na+ and K+ ions. TCP9-like up-regulated the expression of several genes involved in K+/Na+ homeostasis pathway. These findings provide insights into the role of TCP transcription factors in salt stress response in soybean.
Article
Plant Sciences
Shujing Huang, Caihui Chen, Mengxuan Xu, Guibin Wang, Li-an Xu, Yaqiong Wu
Summary: The GbBBX25 gene was isolated from ginkgo and overexpression in Populus trees improved their salt adaptation abilities, resulting in higher soluble sugar levels and peroxidase activity. Five salt stress-related genes were induced in transgenic plants with significantly higher expression levels than in nontransgenic plants.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Lijuan Zhu, Junjie Yang, Yingting Zhang, Hailiang Hu, Jiebing Cui, Jinyu Xue, Jin Xu
Summary: In this study, the CfICE1 gene from Cryptomeria fortunei was transformed into poplar, resulting in improved resistance to cold, drought, and salt stress. The transgenic poplar lines exhibited higher chlorophyll content, antioxidant enzyme activity, and soluble protein content, as well as lower levels of malondialdehyde and hydrogen peroxide. The ultrastructure of the transgenic plants was also more complete compared to the wild type after stress. The upregulation of CfICE1 in transgenic poplars after abiotic stress suggests that it plays a role in regulating the CBF gene and improving plant resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Yandi Wu, Xiucai Fan, Ying Zhang, Jianfu Jiang, Lei Sun, Faiz Ur Rahman, Chonghuai Liu
Summary: VvSNAT1 is a salt-inducible gene highly expressed in leaves, which enhances salt tolerance in plants. It protects cells from oxidative damage by reducing the accumulation of MDA and H2O2.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Ming Li, Rui Chen, Qiyan Jiang, Xianjun Sun, Hui Zhang, Zheng Hu
Summary: GmNAC06 is important in salt stress responses, regulating protein and ion homeostasis, and may be useful in generating salt tolerant transgenic crops.
PLANT MOLECULAR BIOLOGY
(2021)
Article
Genetics & Heredity
Zhipeng Hong, Yang Li, Yang Zhao, Mingyu Yang, Xiaoming Zhang, Yuhan Teng, Linjie Jing, Danxun Kong, Tongxin Liu, Shuanglin Li, Fanli Meng, Qi Wang, Ling Zhang
Summary: In this study, the Arabidopsis gene AtARA6 was transformed into soybean and its effects on salt tolerance pathways were investigated. Transcriptome sequencing revealed that AtARA6 is involved in the regulation of soybean SNARE complexes in the vesicle transport pathway, resulting in enhanced salt tolerance. Furthermore, the downregulation of negative regulator transcription factors MYC2, WRKY6, and WRKY86, along with the enrichment of genes involved in the inositol catabolic process, were observed in transgenic soybeans subjected to salt treatment, leading to reduced reactive oxygen species content and improved salt tolerance.
FRONTIERS IN GENETICS
(2022)
Article
Plant Sciences
Liming Zhu, Mengjuan Li, Junnan Huo, Ziming Lian, Yuxin Liu, Lu Lu, Ye Lu, Zhaodong Hao, Jisen Shi, Tielong Cheng, Jinhui Chen
Summary: NtSOS2, a key factor in plant salt stress response pathway, was cloned from Nitraria tangutorum and found to play an important role in improving plant tolerance to high salt environments. Functional analysis and transgenic experiments confirmed NtSOS2's involvement in regulating ion transporter-related genes and enhancing plant growth under salt stress conditions. These findings provide valuable insights into plant salt tolerance mechanisms and the potential applications of halophyte-derived molecular knowledge for environmental improvement.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Yuwen Zou, Limeng Zhang, Ruicheng Liu, Liqun He, Zhangjian Hu, Yan Liang, Fucheng Lin, Yanhong Zhou
Summary: Endophytic fungus Falciphora oryzae enhances salt tolerance in pepper by promoting ion homeostasis and upregulating antioxidant defense systems. Inoculation with F. oryzae leads to increased plant growth and photosynthetic performance, along with increased synthesis and signaling of indole acetic acid and abscisic acid. This study reveals the potential of endophytic fungi as a strategy to improve plant tolerance to salt stress.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Plant Sciences
Mingyu Yang, Yuhan Teng, Tong Yue, Ziye Wang, Guanghui Feng, Jingwen Ruan, Shi Yan, Yuhong Zheng, Ling Zhang, Qingshan Chen, Fanli Meng
Summary: In this study, the peanut ALDH gene AhALDH2B6 was expressed in soybean using genetic modification, and its function in enhancing cold tolerance was confirmed. Transgenic soybeans showed improved growth and reduced stress-related biochemical indicators. The AhALDH2B6 gene specifically up-regulated genes involved in oxidoreductase-related functions, supporting its role in cold stress alleviation.
Article
Plant Sciences
M. B. Shine, Jung-Wook Yang, Mohamed El-Habbak, Padmaja Nagyabhyru, Da-Qi Fu, Duroy Navarre, Said Ghabrial, Pradeep Kachroo, Aardra Kachroo
Article
Virology
Igor Koloniuk, Mohamed H. El-Habbak, Karel Petrzik, Said A. Ghabrial
ARCHIVES OF VIROLOGY
(2014)
Article
Biochemistry & Molecular Biology
Ajay Kumar Singh, Da-Qi Fu, Mohamed El-Habbak, Duroy Navarre, Said Ghabrial, Aardra Kachroo
MOLECULAR PLANT-MICROBE INTERACTIONS
(2011)
Article
Entomology
Mohamed Hamed El-habbak, Mohamed Hassan Refaat
EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL
(2019)
Meeting Abstract
Plant Sciences
S. Rao, M. El-Habbak, J. S. Haudenshield, D. Zheng, G. L. Hartman, S. S. Korban, S. A. Ghabrial
Meeting Abstract
Plant Sciences
M. H. El-Habbak, A. Padmanaban, S. Ghabrial
Meeting Abstract
Plant Sciences
A. Singh, M. El-Habbak, S. Ghabrial, A. Kachroo