Article
Biochemistry & Molecular Biology
Mei Zhu, Yan Li, Xuesha Long, Congyu Wang, Guiping Ouyang, Zhenchao Wang
Summary: Compound 2-(butyldisulfanyl) quinazolin-4(3H)-one exhibited strong anti-Xac activity and effectively controlled citrus canker. It reduced biofilm formation, increased reactive oxygen species levels, damaged bacterial morphology, and caused bacterial death. Through down-regulation of enzymes in the MEP pathway, this compound showed destructive ability against Xac. Overall, this study suggests that such derivatives hold promise as novel bactericides for citrus canker control.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Andre Vessoni Alexandrino, Evandro Luis Prieto, Nicole Castro Silva Nicolela, Tamiris Garcia da Silva Marin, Talita Alves dos Santos, Joao Pedro Maia de Oliveira da Silva, Anderson Ferreira da Cunha, Franklin Behlau, Maria Teresa Marques Novo-Mansur
Summary: Citrus canker, caused by Xanthomonas citri, is a devastating disease for the citrus industry. Xcc has the ability to use xylose, a plant cell wall constituent, which contributes to its virulence. Deletion of the xylose isomerase genes in Xcc increases its aggressiveness and disease symptoms, indicating that xylose accumulation enhances Xcc virulence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Wenting Li, Zhenxi Li, Jiaquan Huang, Meirong Xu, Zheng Zheng, Xiaoling Deng
Summary: This study provides a reliable CRISPR/cas system for Xcc strains and identifies 15 types of CRISPR arrays with 25 spacers in XccA strains from six provinces in China. The phylogenetic analysis based on the CRISPR locus shows a more reliable and accurate typing result compared to commonly used loci. In addition, seven associated cas genes are found adjacent to the CRISPR array.
HORTICULTURAL PLANT JOURNAL
(2022)
Article
Agronomy
Yun-Jiao Zhang, Yi-Bo Pang, Xin-Yi Wang, Yong-Hui Jiang, Daniela D. Herrera-Balandrano, Yan Jin, Su-Yan Wang, Pedro Laborda
Summary: The application of exogenous genistein inhibited soybean plant growth temporarily, increased disease resistance against Xag and fungal pathogen Sclerotinia sclerotiorum. It also enhanced antioxidant capacity, reduced H2O2 level, and promoted phenolics accumulation in infected leaves. Exogenous genistein altered the endogenous isoflavone profile, leading to increased genistin levels with strong antibacterial activity against Xag.
PEST MANAGEMENT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Doron Teper, Jin Xu, Sheo Shankar Pandey, Nian Wang
Summary: Citrus canker disease caused by Xanthomonas citri subsp. citri is a destructive disease in citrus, with different pathotypes displaying varying host ranges. This study investigated the adaptation of Xcc(AW) strains to incompatible sweet orange, revealing increased expression of type III secretion system and effector genes in adapted strains. Mutations in pthAW1 were identified as contributing to the adaptation of Xcc(AW) to sweet orange, demonstrating active rearrangements of the TALE repeat array during host adaptation.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Article
Multidisciplinary Sciences
He Sun, Xinlu Zhu, Chuanxi Li, Zhiming Ma, Xiao Han, Yuanyuan Luo, Liang Yang, Jing Yu, Yansong Miao
Summary: The study reveals a mechanism by which bacterial T3Es subvert the host actin cytoskeleton through liquid-liquid phase separation, providing insight into the sophisticated strategies pathogens use to manipulate host cell processes.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Camila Fernandes, Leonor Martins, Miguel Teixeira, Jochen Blom, Joel E. Pothier, Nuno A. Fonseca, Fernando Tavares
Summary: The study revealed the presence of different Xanthomonas lineages within the same walnut tree, including pathogenic and nonpathogenic strains. Genome sequencing showed significant genomic differences between X. arboricola pv. juglandis and X. euroxanthea, especially in terms of pathogenicity and virulence features.
Article
Horticulture
Ashok Kumar Mahawer, Anil Kumar Dubey, Om Prakash Awasthi, Dinesh Singh, Anil Dahuja, Amitha Mithra Sevanthi, Amrender Kumar, Amit Kumar Goswami, Nimisha Sharma, Jagdish Yadav, Amit Kumar Kesharwani, Abhijeet Shankar Kashyap, Aditya Kulshreshtha, Ravinder Pal Singh, Amrut Morade, Radha Mohan Sharma
Summary: India is a leading citrus-producing country with significant production of lemons and limes. However, its contribution to the global citrus market is limited due to factors like citrus canker. This study investigated the impact of Xanthomonas citri pv. citri on different Citrus spp. and found that susceptible genotypes showed greater degradation in chlorophyll content and reduction in leaf gas exchange parameters compared to resistant genotypes. Resistant genotypes also exhibited higher activities of antioxidant enzymes and phenolics. The results provide insights into the pathogen mechanism and resistance in Citrus species.
Article
Multidisciplinary Sciences
Simone Cristina Picchi, Mariana de Souza e Silva, Luiz Leonardo Saldanha, Henrique Ferreira, Marco Aurelio Takita, Camila Caldana, Alessandra Alves de Souza
Summary: N-Acetylcysteine (NAC) is known for its antioxidant and anti-adhesive properties, but its antimicrobial activity is less understood. This study showed that NAC affects bacterial cell proliferation and metabolism, particularly in cysteine metabolism. Interestingly, the decrease in amino acids availability due to NAC treatment leads to impaired protein synthesis and cell proliferation.
SCIENTIFIC REPORTS
(2021)
Article
Plant Sciences
Wenting Li, Zhenxi Li, Ling Zhang, Xiaoling Deng, Zheng Zheng
Summary: This study presents the genome sequence of the oldest X. citri pv. citri strain from historical citrus canker specimens in China, which will contribute to the genomic evolution research of X. citri pv. citri.
Article
Biochemistry & Molecular Biology
Analia Carrau, Josefina Tano, Laura Moyano, Maria Belen Ripa, Silvana Petrocelli, Laura Piskulic, Leandro Marcio Moreira, Jose Salvatore Leister Patane, Joao Carlos Setubal, Elena Graciela Orellano
Summary: Plant-pathogen interaction is influenced by environmental factors such as temperature and light. This study shows that the bluf2 gene in Xcc bacterium regulates various aspects of virulence processes, including motility, adhesion, exopolysaccharide production, and biofilm formation. It also regulates ROS detoxification, which plays a role in the plant's response to infection. This is the first report on BLUF-like receptors in plant pathogenic bacteria.
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Swayamjit Ray, Clare L. Casteel
Summary: This review discusses recent advances in effector-mediated plant-virus-vector interactions, suggesting the occurrence of molecular synergisms that benefit both the virus and insect vector. The study reveals that both hemipteran vectors and viruses use effectors to manipulate host physiology and successfully colonize plants. Co-evolutionary dynamics have resulted in effective immune responses in hosts and diverse mechanisms of counterattack by both vectors and viruses. The review also proposes future research directions and methodologies to identify true effector synergisms in plant-virus-vector interactions.
Article
Biotechnology & Applied Microbiology
Dennie te Molder, Wasin Poncheewin, Peter J. Schaap, Jasper J. Koehorst
Summary: The genus Xanthomonas primarily consists of plant pathogens, but there is a growing number of non-pathogenic and endophytic members. Machine learning approaches on genome content can help distinguish between these plant-associated phenotypes. Through literature mining and genome analysis, insights into virulence factors and unique features of non-pathogenic strains can be obtained.
Article
Horticulture
Jyotsana Sharma, Nanjundappa Manjunatha, Somnath S. Pokhare, Prakash G. Patil, Ruchi Agarrwal, Mansi G. Chakranarayan, Anita Aralimar, Priya Devagire, Rajiv A. Marathe
Summary: Xanthomonas axonopodis pv. punicae (Xap) causes bacterial blight disease in pomegranates, resulting in significant economic loss. The lack of resistant varieties and monoculture of susceptible varieties have worsened the problem. Xap has shown low sensitivity to streptomycin under field conditions, and molecular analysis suggests that horizontal gene transfer and gene mutations may be responsible for this lower sensitivity.
Article
Microbiology
Paula M. M. Martins, Thomas K. Wood, Alessandra A. de Souza
Summary: Citrus canker disease, caused by the bacterium Xanthomonas citri subsp. citri, poses a constant threat to citrus-producing areas and requires agricultural practices to limit its spread. Research has shown that X. citri forms dormant cells with reduced metabolism, leading to tolerance to antibiotics. This knowledge could potentially lead to more efficient management practices for controlling the spread of the disease.
Article
Microbiology
Lucas M. Ceseti, Eliane S. de Santana, Camila Y. Ratagami, Yasmin Barreiros, Lidia Dos Passos Lima, German Dunger, Chuck S. Farah, Cristina E. Alvarez-Martinez
CURRENT MICROBIOLOGY
(2019)
Article
Agronomy
C. Jaime, S. E. Muchut, A. G. Reutemann, J. O. Gieco, G. Dunger
Article
Chemistry, Physical
Renato M. Domingos, Raphael D. Teixeira, Ari Zeida, William A. Agudelo, Thiago G. P. Alegria, Jose F. da Silva Neto, Plinio S. Vieira, Mario T. Murakami, Chuck S. Farah, Dario A. Estrin, Luis E. S. Netto
Article
Microbiology
William Cenens, Maxuel O. Andrade, Edgar Llontop, Cristina E. Alvarez-Martinez, German G. Sgro, Chuck S. Farah
Article
Chemistry, Physical
Anacleto Silva de Souza, Jose David Rivera, Vitor Medeiros Almeida, Pingju Ge, Robson Francisco de Souza, Chuck Shaker Farah, Henning Ulrich, Sandro Roberto Marana, Roberto Kopke Salinas, Cristiane Rodrigues Guzzo
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2020)
Article
Microbiology
Edgar E. Llontop, William Cenens, Denize C. Favaro, German G. Sgro, Roberto K. Salinas, Cristiane R. Guzzo, Chuck S. Farah
Summary: The study reveals that in Xanthomonas citri, the ATPase PilB can form a complex with two regulatory proteins, PilZ and c-di-GMP receptor FimX, and these proteins colocalize at the leading poles of the bacterial cells during surface motility. The X-ray structures of PilZ in complex with PilB's N-terminal domain and FimX's C-terminal domain allow for a proposed model of the three-way interaction between these proteins.
Article
Medicine, General & Internal
Gerson Shigeru Kobayashi, Luciano Abreu Brito, Danielle de Paula Moreira, Angela May Suzuki, Gabriella Shih Ping Hsia, Lylyan Fragoso Pimentel, Ana Paula Barreto de Paiva, Carolina Regoli Dias, Naila Cristina Vilaca Lourenco, Beatriz Araujo Oliveira, Erika Regina Manuli, Marcelo Andreetta Corral, Natale Cavacana, Miguel Mitne-Neto, Maria Mirtes Sales, Luiz Phellipe Dell' Aquila, Alvaro Razuk Filho, Eduardo Fagundes Parrillo, Maria Cassia Mendes-Correa, Ester Cerdeira Sabino, Silvia Figueiredo Costa, Fabio Eudes Leal, German Gustavo Sgro, Chuck Shaker Farah, Mayana Zatz, Maria Rita Passos-Bueno
Summary: The study developed a direct RT-LAMP workflow for viral detection in saliva, showing high sensitivity and specificity. Salivary viral load peaks in the first days of symptoms and then decreases. The findings suggest that saliva reflects transmission dynamics better than NOP specimens, and gender differences in viral load may affect transmission rates.
Article
Multidisciplinary Sciences
Gabriel U. Oka, Diorge P. Souza, William Cenens, Bruno Y. Matsuyama, Marcus V. C. Cardoso, Luciana C. Oliveira, Filipe da Silva Lima, Iolanda M. Cuccovia, Cristiane R. Guzzo, Roberto K. Salinas, Chuck S. Farah
Summary: The study identified an interaction between XVIPCD and VirD4, dividing XVIPCD into two regions with distinct functions. The N-terminal region interacts with VirD4, while both the N- and C-terminal regions are required for efficient X-Tfe translocation into target cells. The conformational stability of the N-terminal region is reduced at lower pH, potentially facilitating X-Tfe unfolding and translocation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Lidia dos Passos Lima, Juliana Biar Pereira, Anthony Jhoao Fasabi Flores, Alan Pericles Rodrigues Lorenzetti, Ana Laura Boechat, Maria Claudia Pereda, Sophia Gualtieri, Daniele Ferreira do Prado, Diego Rocha, Lucas de Moraes Ceseti, Regina Lucia Baldini, Chuck S. Farah, Tie Koide, Celso Eduardo Benedetti, Cristina E. Alvarez-Martinez
Summary: The genus Xanthomonas includes multiple phytopathogenic species that infect various plants and cause significant crop diseases. This study characterizes a sigma(ECF) factor called EcfL in Xanthomonas citri, showing its involvement in the virulence and adaptation of the bacterium to citrus plants. EcfL regulates an operon containing three genes and is induced in response to acid stress.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Environmental Sciences
Bianca H. Ventura Fernandes, Natalia Martins Feitosa, Ana Paula Barbosa, Camila Gasque Bomfim, Anali M. B. Garnique, Ivana F. Rosa, Maira S. Rodrigues, Lucas B. Doretto, Daniel F. Costa, Bruno Camargo-dos-Santos, Gabrielli A. Franco, Joao Favero Neto, Juliana Sartori Lunardi, Marina Sanson Bellot, Nina Pacheco Capelini Alves, Camila C. Costa, Mayumi F. Aracati, Leticia F. Rodrigues, Rafaela Hemily Cirilo, Raul Marcelino Colagrande, Francisco I. F. Gomes, Rafael T. Nakajima, Marco A. A. Belo, Percilia Cardoso Giaquinto, Susana Luporini de Oliveira, Silas Fernandes Eto, Dayanne Carla Fernandes, Wilson G. Manrique, Gabriel Conde, Roberta R. C. Rosales, Iris Todeschini, Ilo Rivero, Edgar Llontop, German G. Sgro, Gabriel Umaji Oka, Natalia Fernanda Bueno, Fausto K. Ferraris, Mariana T. Q. de Magalhaes, Renata J. Medeiros, Juliana M. Mendonca-Gomes, Mara Souza Junqueira, Katia Conceicao, Leticia Gomes de Pontes, Antonio Condino-Neto, Andrea C. Perez, Leonardo J. G. Barcellos, Jose Dias Correa Junior, Erick Gustavo Dorlass, Niels O. S. Camara, Edison Luiz Durigon, Fernando Q. Cunha, Rafael H. Nobrega, Glaucia M. Machado-Santelli, Chuck S. Farah, Flavio P. Veras, Jorge Galindo-Villegas, Leticia Costa-Lotufo, Thiago M. Cunha, Roger Chammas, Luciani R. Carvalho, Cristiane R. Guzzo, Guilherme Malafaia, Ives Charlie-Silva
Summary: This study found that injection of SARS-CoV-2 Spike protein rSpike in zebrafish resulted in mortality and adverse effects on liver, kidney, ovary, and brain tissues, with a survival rate of 78.6%. The conserved genetic homology between zebrafish and humans may contribute to the intense toxic effects observed. The results highlight the potential of zebrafish as an animal model for translational COVID-19 research and for understanding ecotoxicological impacts, vaccine responses, and therapeutic approaches.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biotechnology & Applied Microbiology
Santiago Justo Arevalo, Daniela Zapata Sifuentes, Andrea Cuba Portocarrero, Michella Brescia Reategui, Claudia Monge Pimentel, Layla Farage Martins, Paulo Marques Pierry, Carlos Morais Piroupo, Alcides Guerra Santa Cruz, Mauro Quinones Aguilar, Chuck Shaker Farah, Joao Carlos Setubal, Aline Maria da Silva
Summary: Understanding the biochemistry and metabolic pathways of cyanide degradation is crucial for improving cyanide bioremediation processes. In this study, a cyanide-degrading Bacillus strain was isolated and sequenced from water in contact with mine tailings in Peru. The genome analysis identified a putative cyanide dihydratase gene, and the recombinant enzyme showed activity at high pH. Transcription of the gene was strongly induced in the presence of cyanide. These findings suggest that this Bacillus strain and its cyanide dihydratase enzyme could be potential tools for cyanide bioremediation.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Rosa Maria Chura-Chambi, Chuck Shaker Farah, Ligia Morganti
Summary: We have demonstrated that hGH-IBs possess native-like secondary and tertiary structures and that non-denaturing methods can lead to high yields of refolded protein. The refolding process described in this study is likely applicable to other proteins, and it may be particularly useful for reducing the pH required for alkaline solubilization.
MICROBIAL CELL FACTORIES
(2022)
Article
Biochemistry & Molecular Biology
Lidia dos Passos Lima, Eliane Silva de Santana, Alan Pericles Rodrigues Lorenzetti, Rogerio Ferreira Lourenco, Lucas de Moraes Ceseti, Luana Riva, Ethel Bayer-Santos, Ana Laura Boechat, Regina Lucia Baldini, Chuck Shaker Farah, Tie Koide, Cristina Elisa Alvarez-Martinez
Summary: This study uncovers the regulatory mechanism of X. citri T6SS. Overexpression of a constitutively active EcfK activates the transcription factor TagK, which induces the expression of T6SS genes. TagK binds directly to T6SS gene promoters and promotes T6SS expression upon contact with amoeba cells.
MOLECULAR MICROBIOLOGY
(2022)
Article
Biology
Julia Takuno Hespanhol, Daniel Enrique Sanchez-Limache, Gianlucca Goncalves Nicastro, Liam Mead, Edgar Enrique Llontop, Gustavo Chagas-Santos, Chuck Shaker Farah, Robson Francisco de Souza, Rodrigo da Silva Galhardo, Andrew L. Lovering, Ethel Bayer-Santos
Summary: This study characterizes the function of the SPI-22 T6SS in Salmonella bongori, highlighting its antibacterial activity, and identifies a group of antibacterial T6SS effectors (TseV1-4) evolutionarily related to DNA repair enzymes. Specifically, TseV3 is able to recognize and cleave specific DNA structures, generating DNA double-strand breaks and inducing the SOS response in target cells. The crystal structure of the TseV3:TsiV3 complex suggests that the immunity protein blocks the effector's interaction with the DNA substrate.
Review
Biochemistry & Molecular Biology
Cristina E. Alvarez-Martinez, German G. Sgro, Gabriel G. Araujo, Mateus R. N. Paiva, Bruno Y. Matsuyama, Cristiane R. Guzzo, Maxuel O. Andrade, Chuck S. Farah
Summary: This study describes the diversity of roles played by different types of secretion systems in adaptation and virulence in Xanthomonas, as well as the distribution and functions associated with these important nanoweapons within the genus.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)