Article
Chemistry, Analytical
James W. Beattie, Alena Istrate, Annabelle Lu, Cameron Marshall, Ruth C. Rowland-Jones, Monika Farys, Sergei G. Kazarian, Bernadette Byrne
Summary: Monoclonal antibodies (mAbs) are widely used biotherapeutics, but their production is time-consuming and expensive. This study used Raman spectroscopy to investigate the binding behavior and capacity loss of protein A resin. The results revealed a distinct Raman spectral fingerprint for mAb bound to the protein A ligand, and showed that the decrease in binding capacity could be detected by Raman spectroscopy. Depth profiling analysis indicated that mAb binding to used resin beads is not homogeneous. The presence of irreversibly bound mAb and host cell proteins was confirmed by mass spectrometric analysis.
ANALYTICAL CHEMISTRY
(2022)
Article
Microbiology
Yuuki Kodama, Shoya Miyazaki
Summary: The endosymbiosis between Paramecium bursaria and Chlorella spp. is mutualistic, with symbiotic algae potentially promoting host cell survival under starvation conditions.
CURRENT MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Mario Costa, Ana Marta Pereira, Silvia Coimbra
Summary: Plant development involves constant adjustments of cell wall composition and structure, with the use of fluorescent immunolocalization offering a high-resolution and specific technique to detect multiple glycan epitopes in the same sample, guiding research and linking glycans to specific functions in plants.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Plant Sciences
Sukhita Sathitnaitham, Anongpat Suttangkakul, Passorn Wonnapinij, Simon J. McQueen-Mason, Supachai Vuttipongchaikij
Summary: Cell walls are dynamic, multi-component materials that are crucial in plant biology, with their properties modulated by wall-related enzymes. A new method allows simultaneous examination of specific cell wall matrix components, providing insight into direct and indirect consequences of cell wall manipulations. By utilizing this method, researchers can gain a deeper understanding of wall composition, its dynamism, and plasticity, further advancing knowledge of cell wall biology.
Article
Biochemistry & Molecular Biology
Sunaina Kiran Prabhu, Chao Li, Guanghui Zong, Roushu Zhang, Lai-Xi Wang
Summary: Core fucosylation plays a crucial role in modulating the structure and biological functions of glycoproteins, but only a few alpha-L-fucosidases are capable of removing core fucose from intact glycoproteins. This study found that bacterial alpha-L-fucosidases from Lactobacillus casei and Bacteroides fragilis could only hydrolyze truncated Fuc alpha 1,6GlcNAc-peptide substrates, while the human alpha-L-fucosidase showed activity in removing core fucose from intact glycopeptides and glycoproteins. Additionally, the human alpha-L-fucosidase was the only enzyme that exhibited some capability to remove core fucose from intact IgG antibodies.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Plant Sciences
Kaewta Rattanapisit, Tharita Kitisripanya, Atthaphon Konyanee, Worapol Sae-Foo, Apisit Burapapiruin, Waraporn Putalun, Seiichi Sakamoto, Waranyoo Phoolcharoen, Gorawit Yusakul
Summary: The plant expression platform is a new source for producing IgG targeting small low-molecular-weight molecules. The study demonstrated that plant-produced functional IgG maintained the binding activity and specificity of IgG derived from mammalian cells, showing potential as an efficient and cost-effective platform for antibody production in immunoassays targeting low-molecular-weight compounds.
PLANT CELL REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Xihua Lian, Stephen Chambers, John G. Lewis, Amy Scott-Thomas, Madhav Bhatia
Summary: Invasive aspergillosis (IA) is a life-threatening disease mainly caused by Aspergillus fumigatus and Aspergillus flavus. Current diagnostic techniques for IA lack accuracy. Two monoclonal antibodies (1D2 and 4E4) provide a potentially promising diagnostic approach. These antibodies showed strong immunoreactivity against A. fumigatus and A. flavus. The antigens identified may be glycoproteins and the immunoreactivity is strong.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Veterinary Sciences
Ning Xiao, Jiannan Li, Minxue Li, Yuting Hu, Huixing Lin, Hongjie Fan
Summary: This study successfully produced and identified three highly specific monoclonal antibodies against the outer membrane protein (Omp2) of L. intracellularis for the detection of the bacteria in infected cells and ileal tissues. The results show that these monoclonal antibodies can be useful tools for evaluating L. intracellularis infection in vivo and in vitro.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Biochemical Research Methods
Lav Tripathi, Eero Hietanen, Pirjo Merilahti, Laura Teixido, Neus Sanchez-Alberola, Sisko Tauriainen, Petri Susi
Summary: This study successfully cloned VP4 and VP2 genes from human parechovirus A1 (PeV-A1) and developed monoclonal antibody Mab-PAR-1 with broad reactivity against parechoviruses, showing potential for application in rapid antigen detection assays.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Review
Medicine, Research & Experimental
Omozojie P. Aigbogun, Christopher P. Phenix, Ed S. Krol, Eric W. Price
Summary: Small-molecule drugs have short in vivo half-lives, hindering their success as therapeutics. Chemically programmed antibodies (cPAbs) have been developed to address these limitations by augmenting small molecules with antibodies for effector functions and prolonged pharmacokinetic profiles. This review summarizes the different methods used to produce cPAbs and highlights their potential applications in antibody modification fields.
MOLECULAR PHARMACEUTICS
(2023)
Article
Chemistry, Multidisciplinary
Shravan Sreenivasan, Deepak Sonawat, Anurag S. Rathore
Summary: An established platform for determining the size distribution of therapeutic IgG aggregates utilized image processing algorithms for brightfield and fluorescence microscope images. The algorithm involved conversion to grayscale, thresholding, morphological operations, and denoising steps for fluorescence images of tagged aggregates. The resulting size distribution was compared with experimental techniques, showing potential as an orthogonal methodology for analyzing IgG aggregates.
PHARMACEUTICAL RESEARCH
(2021)
Article
Food Science & Technology
Cristina Capodicasa, Erica Bastiani, Thea Serra, Laura Anfossi, Marcello Catellani
Summary: A new green competitive ELISA method for the quantification of aflatoxin M1 in raw milk, based on plant-produced recombinant monoclonal antibodies, has been successfully developed. The method showed high accuracy within the decision levels required by EU legislation, with a detection limit of 3 ng/L and a quantification range of 5-110 ng/L.
Article
Chemistry, Applied
Jingming Zhou, Wenjing Qian, Qingbao Yang, Chao Liang, Yumei Chen, Aiping Wang, Gaiping Zhang
Summary: A quantum dots-based fluorescent immunochromatographic assay (QDs-ICA) was developed for the sensitive analysis of VIR M1. The QDs-ICA exhibited higher sensitivity compared to existing methods and showed potential for analyzing VIR M1 in animal feed and animal-derived food.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2022)
Article
Immunology
Collin Jugler, Haiyan Sun, Francisca Grill, Karen Kibler, Adrian Esqueda, Huafang Lai, Yize Li, Douglas Lake, Qiang Chen
Summary: In this study, researchers developed an antibody cocktail against SARS-CoV-2 using Nicotiana benthamiana plants. The cocktail, consisting of two monoclonal antibodies, showed high expression in plants and effectively neutralized multiple variants of the virus. This work demonstrates the potential of using plant-based production for the development of affordable and efficacious mAb cocktails against emerging viral diseases.
Article
Food Science & Technology
Yiheng Shi, Lei Zhou, Xiaowei Qu, Tianli Yue, Yahong Yuan
Summary: An effective method for identifying A. acidoterrestris using is-ELISA was developed, which is highly sensitive and specific, suitable for preventing juice spoilage.
Article
Forestry
Maree Brennan, Clement Fritsch, Sylvain Cosgun, Stephane Dumarcay, Francis Colin, Philippe Gerardin
ANNALS OF FOREST SCIENCE
(2020)
Article
Engineering, Aerospace
George D. O'Mahony, James T. Curran, Philip J. Harris, Colin C. Murphy
IEEE AEROSPACE AND ELECTRONIC SYSTEMS MAGAZINE
(2020)
Article
Forestry
Maree Brennan, David Hentges, Sylvain Cosgun, Stephane Dumarcay, Francis Colin, Christine Gerardin, Philippe Gerardin
Summary: Softwood species' knotwood is rich in lignans and terpenes, with D-pinitol also present in high concentrations at the crown top. Different softwood species exhibit variability in extractive yields and compositions, with potential bioactive molecules for anti-tumor properties and food-supplement markets.
Article
Food Science & Technology
Aaron Hoyle, Maree Brennan, Nicholas Pitts, Gail E. Jackson, Steve Hoad
JOURNAL OF CEREAL SCIENCE
(2020)
Article
Plant Sciences
Maree Brennan, Clement Fritsch, Sylvain Cosgun, Stephane Dumarcay, Francis Colin, Philippe Gerardin
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2020)
Article
Plant Sciences
Kathryn R. Grant, Maree Brennan, Stephen P. Hoad
Summary: This study investigates the relationship between genotype, plant development, plant structure, and plant material properties in barley husks. It finds that genotype influences lemma structure and material properties, which in turn affect the risk of grain skinning. Thinner lemma structures in high-risk genotypes are more prone to fragmentation, potentially explaining the differences in grain skinning risk between genotypes.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Computer Science, Information Systems
George D. O'Mahony, Kevin G. McCarthy, Philip J. Harris, Colin C. Murphy
Summary: This paper focuses on improving wireless edge device security by developing a novel intelligent interference diagnostic framework using I/Q samples. By utilizing low-order features, high accuracy and generalization to unseen data are achieved. The study establishes the foundation for low complexity supervised machine learning models for interference detection in wireless networks.
Article
Plant Sciences
Ramesh R. Chavan, Adya P. Singh, Awanis Azizan, Philip J. Harris
Summary: The predominant hemicelluloses in the gametophytic stem of moss Hypnodendron menziesii were found to be heteromannans. Immunofluorescence and immunogold microscopy confirmed the presence of heteromannans in all cell types, except hydroids, with secondary walls. Pre-treatments were essential for detecting heteromannans, as revealed by experiments with sodium carbonate and pectate lyase.
Article
Forestry
Jean-Baptiste Pichancourt, Rodolphe Bauer, Antoine Billard, Maree Brennan, Sylvain Caurla, Antoine Colin, Adrien Contini, Sylvain Cosgun, Henri Cuny, Stephane Dumarcay, Mathieu Fortin, Philippe Gerardin, David Jean Hentges, Fleur Longuetaud, Beatrice Richard, Holger Wernsdorfer, Francis Colin
Summary: This study presents a methodological framework for organizing information and making informed decisions about extracting bio-molecules from forest biomass. By demonstrating its usefulness in extracting bio-molecules from silver fir in France, it highlights the challenges faced by supply chain actors in decision-making processes. The study emphasizes the need for future improvements to the methodological framework to address the challenges in decision-making regarding the extraction of high-value bio-molecules.
ANNALS OF FOREST SCIENCE
(2021)
Article
Plant Sciences
Xuezhao Sun, Ian G. Andrew, Philip J. Harris, Simone O. Hoskin, Keith N. Joblin, Yuhua He
Summary: This study mapped the distribution of pectic polysaccharides in the cell walls of forage chicory leaves using four monoclonal antibodies. The differential distribution of pectic epitopes among walls of different cell types and within walls may reflect the deposition and modification of these polysaccharides which are involved in cell wall properties and cell development.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Oncology
Karin Purshouse, John P. Thomson, Maheva Vallet, Lorna Alexander, Isaac Bonisteel, Maree Brennan, David A. Cameron, Jonine D. Figueroa, Elizabeth Furrie, Pamela Haig, Mattea Heck, Hugh McCaughan, Paul Mitchell, Heather McVicars, Lorraine Primrose, Ines Silva, Kate Templeton, Natalie Wilson, Peter S. Hall
Summary: This article characterizes the incidence and outcomes of SARS-CoV-2 infection and vaccination in patients with solid tumors undergoing anti-cancer treatment. The study found that patients undergoing anti-cancer treatment have high protection from SARS-CoV-2 infection following COVID-19 vaccination.
Article
Plant Sciences
Shih-Yi Hsiung, Jing Li, Balazs Imre, Mu-Rong Kao, Hsien-Chun Liao, Damao Wang, Chih-Hui Chen, Pi-Hui Liang, Philip J. Harris, Yves S. Y. Hsieh
Summary: The xyloglucans of aquatic Araceae species have unique structures compared to other non-commelinid monocotyledon families. Lemna minor, a species of aquatic Araceae, was found to have xyloglucans with a different structure from other non-commelinid monocotyledons. A study of 26 Araceae species, including L. minor, revealed that all seven aquatic species examined had xyloglucans with one or two of three distinct features: a < 77% XXXG core motif, no fucosylation, and > 14% oligosaccharide units with S or D side chains. Two terrestrial species also lacked fucose in their xyloglucans, indicating this feature is not unique to aquatic species.
Article
Environmental Sciences
Ninna Granucci, Philip J. Harris, Silas G. Villas-Boas
Summary: This study analyzed the chemical composition of apple, orange, and carrot pomaces and found that dietary fiber was the most abundant component, mainly consisting of cellulose and pectin. In addition, soluble sugars were also major components of the pomaces. The study concluded that these food-grade pomaces have great potential for bioconversion into high-value-added products through microbial fermentation.
WASTE AND BIOMASS VALORIZATION
(2023)
Proceedings Paper
Automation & Control Systems
George D. O'Mahony, Philip J. Harris, Colin C. Murphy
2020 31ST IRISH SIGNALS AND SYSTEMS CONFERENCE (ISSC)
(2020)
Article
Plant Sciences
Aaron Hoyle, Maree Brennan, Logan Rees, Gail E. Jackson, Stephen P. Hoad