Review
Cell Biology
Huaqiang Ruan, Jiang Li, Ting Wang, Haiyun Ren
Summary: Pollen germination and pollen tube growth are important biological events in higher plants' sexual reproduction, involving vesicle trafficking, membrane fusion, and regulation by cytoskeleton, proteins, and signaling molecules such as small G proteins, calcium, and PIP2. Researchers focus on understanding how regulators and signaling molecules participate in secretory vesicle delivery, tethering, and fusion during pollen germination and tube growth.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Sebastian Eising, Bianca Esch, Mike Waelte, Prado Vargas Duarte, Stefan Walter, Christian Ungermann, Maria Bohnert, Florian Froehlich
Summary: Lysosome is a crucial catabolic organelle in cells, with mutations in lysosomal proteins potentially leading to neurodegeneration, cancer, and age-related diseases. The vacuole serves as a lysosomal analog in Saccharomyces cerevisiae, containing evolutionarily conserved proteins. Proteins can reach vacuoles through different pathways, including the Vps10-dependent endosomal vacuolar protein sorting pathway, the alkaline phosphatase pathway, and the cytosol-to-vacuole transport pathway.
JOURNAL OF CELL BIOLOGY
(2022)
Review
Plant Sciences
Stephen J. Bordeleau, Laura Canales E. Sanchez, Daphne R. Goring
Summary: Successful fertilization of a flowering plant requires cell-to-cell communication between pollen grain and pistil, where ligand-receptor kinase signaling plays a crucial role. While the later stages of pollen-pistil interactions have been well studied in Arabidopsis, the earlier stages remain less clear. Recent studies have implicated predicted receptor kinases in regulating pollen hydration and growth in the upper regions of the pistil. However, many questions regarding their specific functions remain unanswered.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Yazhou Duan, Limin Wang, Xueling Li, Wanlei Wang, Jing Wang, Xiaoyu Liu, Yangyang Zhong, Nana Cao, Mengjuan Tong, Weina Ge, Yi Guo, Rui Li
Summary: SKS11 and SKS12 are crucial for regulating the integrity, growth, and guidance of pollen tubes, possibly by modulating ROS levels and the deposition or remodeling of cell wall polysaccharides.
Article
Plant Sciences
Natalia Serrano, Premysl Pejchar, Hana Soukupova, Martin Hubalek, Martin Potocky
Summary: This study reveals the changes in whole-cell glycerolipid composition during tobacco pollen germination and growth, confirming the important role of phosphatidic acid in the establishment and maintenance of cellular polarity in pollen tubes, and suggesting the involvement of plasma membrane lysophospholipids in pollen germination.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Natalia V. Ozolina, Irina S. Kapustina, Veronika V. Gurina, Ekaterina V. Spiridonova, Vadim N. Nurminsky
Summary: The study compares the changes in lipid content of plant cell boundary membranes under hyperosmotic stress and highlights the significant role of the vacuolar membrane in the stress response.
Article
Plant Sciences
Lei Yang, Liwen Jiang
Summary: Vacuolar sorting is crucial for plants and plays a role in important agricultural traits. This study focused on the subcellular localization of seven vacuolar sorting receptors (VSRs) in rice and confirmed their role as markers for the prevacuolar compartment/multivesicular body (PVC/MVB) in rice.
JOURNAL OF PLANT PHYSIOLOGY
(2023)
Article
Cell Biology
Jan Zouhar, Wenhan Cao, Jinbo Shen, Enrique Rojo
Summary: The study of endomembrane trafficking is crucial for understanding cellular and organism functionality. Investigating endomembrane trafficking in plants is of particular interest due to its role in transporting seed storage proteins and secreting cell wall material. While the mechanisms of anterograde transport in plants have been extensively discussed, retrograde trafficking pathways have received less attention. This review summarizes the current understanding of retrograde trafficking in the endomembrane system of plants, discussing its integration with anterograde transport, describing retrieval mechanisms, highlighting contentious issues, and identifying future research questions.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Hsi-En Tsao, Shu Nga Lui, Anthony Hiu-Fung Lo, Shuai Chen, Hiu Yan Wong, Chi-Kin Wong, Liwen Jiang, Kam-Bo Wong
Summary: In Arabidopsis, vacuolar sorting receptor isoform 1 (VSR1) plays a key role in directing 12S globulins and other storage proteins to the protein storage vacuoles during seed development. The crystal structure of VSR1-PA in complex with the C-terminal pentapeptide of cruciferin 1 sheds light on the molecular mechanisms involved in the sorting process. The study provides insights into how VSR1 recognizes and interacts with the C-terminal sorting determinants of various storage proteins, which could potentially aid in targeting recombinant proteins to seed storage vacuoles.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Cerrone Cabanos, Yuki Matsuoka, Nobuyuki Maruyama
Summary: This review article focuses on the major seed storage proteins of soybean, their three-dimensional structures, nutritional importance, and bioactive peptides. It also summarizes past efforts in the recombinant production of foreign proteins or bioactive peptides in soybean seed.
Article
Biotechnology & Applied Microbiology
Su Yan, Yan Xu, Xiu-Mei Tao, Xiao-Wei Yu
Summary: It was found that knocking out vps10, vps13, and vps21 genes in T. reesei improved cellulase production by alleviating vacuolar transport and degradation. Transcriptome analysis indicated that increased cellulase gene expression might be attributed to the differential expression of sugar transporters. This study provides new clues for higher cellulase production in T. reesei through the alleviation of vacuolar transport via knockout of vps10, vps13, and vps21.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemical Research Methods
Yasen Wang, Buqing Wang, Yahui Gao, Hideki Nakanishi, Xiao-Dong Gao, Zijie Li
Summary: This study achieved highly efficient expression and secretion of human lysozyme (hLYZ) in Pichia pastoris using multiple strategies. The highest hLYZ activity reached 352,000 U/mL in a 5L fermenter. This research provides a cost-effective approach for hLYZ production and can be applied to the production of other recombinant proteins.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Plant Sciences
Chuanliang Liu, Yonglun Zeng, Hongbo Li, Chao Yang, Wenjin Shen, Min Xu, Zhidan Xiao, Tongsheng Chen, Baiying Li, Wenhan Cao, Liwen Jiang, Marisa S. Otegui, Caiji Gao
Summary: The study identified FYVE4 as a novel plant endosomal regulator, functioning in the ESCRT pathway to regulate MVE biogenesis.
Article
Cell Biology
Ilenia Agliarulo, Seetharaman Parashuraman
Summary: This article discusses the central role of the Golgi apparatus in the mammalian secretory pathway and its regulation of plasma membrane biosynthesis through three distinct processes. It also explores the importance of the molecules involved in these processes in physiology and development, as well as how mutations in these molecules can lead to genetic diseases and cancer.
Article
Plant Sciences
Cecilia M. Lara-Mondragon, Alexandria Dorchak, Cora A. MacAlister
Summary: In Arabidopsis pollen tubes, class I formins play a crucial role in organizing actin arrays during tube elongation, with their localization and mobility being dependent on O-glycosylation of their extracellular domain. The extracellular domain is necessary for the function of two pollen-expressed class I formins, AtFH3 and AtFH5, and specific O-glycans are attached to these sequences. Loss of O-glycosylation alters membrane localization and disrupts actin organization. The different O-glycans also affect lateral mobility in the plasma membrane. This study highlights the importance of protein sub-functionalization in pollen tube elongation.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Meeting Abstract
Oncology
Lin Haifeng, Ma Jie, Zhuo Manyun, Zhang Chengsheng, Luo Jingru, Zhuang Xiaohong, Zeng Zhiming, Yang Lihua
JOURNAL OF CLINICAL ONCOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Lok Wai Cola Ho, Cecilia Ka Wing Chan, Ruifang Han, Yolanda Fong Yung Lau, Huize Li, Yi-Ping Ho, Xiaohong Zhuang, Chung Hang Jonathan Choi
Summary: Understanding the mechanism of nanoparticle exocytosis from cells is important for designing nanoparticles with desirable cellular retention for nanomedicine applications. This study investigates the exocytosis mechanism of dodecyl-terminated, polyethylene glycol-coated gold nanoparticles and finds that the presence of alkyl chains promotes cellular uptake and improves exocytosis efficiency.
Article
Cell Biology
Shuangli Sun, Lanlan Feng, Kin Pan Chung, Ka-Ming Lee, Hayley Hei-Yin Cheung, Mengqian Luo, Kaike Ren, Kai Ching Law, Liwen Jiang, Kam-Bo Wong, Xiaohong Zhuang
Summary: The study uncovered an atypical interaction mode of Arabidopsis ATG8f with the plant unique adaptor protein SH3P2, showing unique conformational changes in ATG8f upon binding to the AIM sequence of the autophagic receptor NBR1. Additionally, the gel filtration assay revealed that the ubiquitin-associated domain of NBR1 outcompetes the SH3 domain of SH3P2 for ATG8f interaction. Biochemical and cellular analysis demonstrated distinct interfaces used by ATG8f to interact with NBR1 and SH3P2.
Meeting Abstract
Oncology
Haifeng Lin, Jie Ma, Manyun Zhuo, Chengsheng Zhang, Jingru Luo, Xiaohong Zhuang, Zhiming Zeng, Lihua Yang
JOURNAL OF CLINICAL ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hongbo Li, Yanglan Liao, Xuanang Zheng, Xiaohong Zhuang, Caiji Gao, Jun Zhou
Summary: This review discusses the role of phosphorylation in the regulation of autophagosome biogenesis and autophagic degradation in plants, and summarizes the experimentally verified phosphorylation sites in the core autophagy machinery in plants. Several approaches to test the roles of phosphorylation in the regulation of plant autophagy are also proposed.
Article
Plant Sciences
Hongbo Li, Juan Wei, Yanglan Liao, Xiaoling Cheng, Shuhong Yang, Xiaohong Zhuang, Zhonghui Zhang, Wenjin Shen, Caiji Gao
Summary: This study reveals a previously unidentified function of plant-specific casein kinase I members in attenuating ABA signaling by regulating the phosphorylation and nuclear accumulation of FREE1.
PLANT CELL AND ENVIRONMENT
(2022)
Review
Plant Sciences
Kai Ching Law, Ka Kit Chung, Xiaohong Zhuang
Summary: Endomembrane trafficking is a fundamental and essential process for the transportation of cellular components in all eukaryotic organisms. In plant cells, the Golgi apparatus and trans-Golgi network play crucial roles in sorting and trafficking. Recent studies in plants have provided novel insights into the sorting mechanisms involving adaptor protein complexes, retromer, and retriever complexes.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Cell Biology
Yunting Zhang, Fan Ying, Xiaoyu Tian, Zhenchuan Lei, Xiao Li, Chun-Yin Lo, Jingxuan Li, Liwen Jiang, Xiaoqiang Yao
Summary: TRPM2 plays a promoting role in the progression of hypercholesterolemia-induced atherosclerosis by linking ROS to Ca2+ and inflammation.
Article
Cell Biology
He Yan, Menglong Zhuang, Xiaoyu Xu, Shanshan Li, Mingkang Yang, Nianle Li, Xiaojuan Du, Kangwei Hu, Xiaomin Peng, Wei Huang, Hong Wu, Yu Chung Tse, Lifeng Zhao, Hao Wang
Summary: The study reveals that autophagy plays a significant role in plant sexual reproduction and is essential for pollen tube growth and male fertility in Arabidopsis. Additionally, the research demonstrates the interaction between autophagosomes and prevacuolar compartments and the involvement of autophagy in mitochondrial quality control.
Article
Biophysics
Ruolan Zhou, Xiaohong Zhuang, Qiaoli Wu, Ming Jin, Chaochuan Zheng, Yayun Jiang, Yongliang Lou, Laibao Zheng
Summary: In this study, an artificial enzyme Cu-MOF@Pt based on nanomaterials was successfully prepared, which demonstrated its selective detection ability for glucose. This method has broad application prospects.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Cell Biology
Yilin He, Jiayang Gao, Mengqian Luo, Caiji Gao, Youshun Lin, Hiu Yan Wong, Yong Cui, Xiaohong Zhuang, Liwen Jiang
Summary: This study identified and characterized two Arabidopsis SNAREs, AT4G15780/VAMP724 and AT1G04760/VAMP726, involved in plant autophagy. Phenotypic analysis showed that mutants of VAMP724 and VAMP726 are sensitive to nutrient-starved conditions. Further experiments demonstrated that VAMP724 and VAMP726 regulate autophagosome formation likely working together with ATG9 in Arabidopsis.
Article
Multidisciplinary Sciences
Hongbo Li, Ruihua Huang, Yanglan Liao, Shuhong Yang, Bojin Feng, Hongting Qin, Jun Zhou, Yonglun Zeng, Jinbo Shen, Xiaohong Zhuang, Liwen Jiang, Marisa S. Otegui, Shengchun Zhang, Caiji Gao
Summary: The study identifies a plant-specific endosomal regulator, BLISTER (BLI), that interacts with the retromer complex and plays a crucial role in regulating endosomal trafficking in plants.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Plant Sciences
Mengqian Luo, Kai Ching Law, Yilin He, Ka Kit Chung, Muk Kuen Po, Lanlan Feng, Kin Pan Chung, Caiji Gao, Xiaohong Zhuang, Liwen Jiang
Summary: This study reveals the role of AUTOPHAGY-RELATED2 in coordinating the trafficking of ATG18a and ATG9 to mediate autophagosome closure in Arabidopsis. Under normal conditions, ATG2 is essential for the transport of ATG18a and ATG9, and its depletion leads to unclosed autophagosome structures.
Article
Biochemistry & Molecular Biology
Xun Weng, Yifan Shen, Liwen Jiang, Lifeng Zhao, Hao Wang
Summary: Using fluorescence recovery after photobleaching, we observed the occurrence and localization of rapid vesicular activities in the tip region of growing pollen tube. We demonstrated that both vesicle polar exocytic fusion and endocytosis take place at the apex of the pollen tube. Furthermore, we found that the dynamic activities of tip-focused exocytosis and endocytosis are closely correlated with the spatial organization of actin fringe.
Article
Plant Sciences
Jun Zhou, Juncai Ma, Chao Yang, Xiu Zhu, Jing Li, Xuanang Zheng, Xibao Li, Siyu Chen, Lei Feng, Pengfei Wang, Man Ip Ho, Wenlong Ma, Jun Liao, Faqiang Li, Chao Wang, Xiaohong Zhuang, Liwen Jiang, Byung-Ho Kang, Caiji Gao
Summary: Short-term acute heat stress causes vacuolation of the Golgi apparatus, whereafter autophagy-related protein 8 (ATG8) translocates to the dilated Golgi membrane. The inactivation of the ATG conjugation system abolishes the targeting of ATG8 to the swollen Golgi, causing a delay in Golgi recovery after heat stress. CLATHRIN LIGHT CHAIN 2 (CLC2) is recruited to the cisternal membrane by ATG8, facilitating Golgi reassembly. This study reveals a hitherto unanticipated process of Golgi stack recovery from heat stress in plant cells and uncovers a previously unknown mechanism of organelle resilience involving ATG8.