Article
Plant Sciences
Jun Li, Ryan Foster, Si Ma, Sheng-Jin Liao, Samuel Bliss, Dewi Kartika, Lu Wang, Limin Wu, Andrew L. Eamens, Yong-Ling Ruan
Summary: Cell wall invertase (CWIN) hydrolyzes sucrose into glucose and fructose in the extracellular matrix and plays crucial roles in assimilate partitioning and sugar signaling. A study identified a group of transcription factors (TFs) that modulate CWIN gene expression in Arabidopsis thaliana, with MYB21, ARF6, ARF8, AP3 and CRC shown to be the most likely regulators. The involvement of these TFs in regulating CWIN expression provides insight into potential targets for engineering assimilate allocation and reproductive development to improve seed yield.
Article
Plant Sciences
Fei Li, Xiao Han, Huan Guan, Mei Chen Xu, Yu Xiu Dong, Xin-Qi Gao
Summary: In this study, a gene called PALD was identified in Arabidopsis thaliana and found to play a critical role in pollen longevity. PALD is expressed specifically in mature pollen grains and the pollen tube, and its expression is upregulated under dry conditions. The protein encoded by PALD is associated with lipid droplets (LDs), and the presence of PALD is important for maintaining LD quality and regulating pollen longevity. The study also showed that loss-of-function PALD mutants had reduced LDs in pollen grains and compromised pollen germination and male transmission after being stored under dry conditions.
Article
Plant Sciences
Xinlong Chen, Yingying Zhang, Wuzhong Yin, Gang Wei, Hailing Xu, Lu Ma, Weijiang Tian, Guang Yang, Yunfeng Li, Renhong Wu, Ting Zhang, Nan Wang, Guanghua He
Summary: EFOP3 and EFOP4 are key genes involved in regulating pollen development in Arabidopsis. They play important roles in vasculature formation, cell wall metabolism, and pollen grain development. This study provides insights into the expression, localization, and essentiality of EFOP3 and EFOP4 in pollen development, as well as their impact on Arabidopsis pollen fertility.
Article
Plant Sciences
Lang Yang, Jinhong Liu, Ching-Kiu Wong, Boon Leong Lim
Summary: The growth of pollen tubes relies on actin filaments for reproduction in plants. Pharmacological experiments indicate that the trafficking of lipid droplets (LDs) in pollen tubes is related to F-actin. Additionally, Myo11C1 and Myo11C2 motors were found to be involved in LDs movement, sharing functional redundancy in Arabidopsis pollen tubes.
Article
Biochemistry & Molecular Biology
Ludi Wang, Yui-Leung Lau, Lian Fan, Maurice Bosch, James Doughty
Summary: The pollen coat, derived from the anther tapetum, plays a central role in early pollination events by mediating molecular recognition and is rich in small cysteine-rich proteins (CRPs). Proteomic profiling of Arabidopsis thaliana, Arabidopsis lyrata, and Brassica oleracea revealed a large number of previously unidentified small CRPs in the pollen coat. Additionally, a wide range of other protein families associated with signal transduction, cell walls, lipid metabolism, and defense were also found in the proteomes. These findings provide valuable molecular targets for further studies on the pollen-stigma interaction and its evolutionary links to plant-pathogen interactions.
Article
Multidisciplinary Sciences
Haonan Lin, Hyeon Jeong Lee, Nathan Tague, Jean-Baptiste Lugagne, Cheng Zong, Fengyuan Deng, Jonghyeon Shin, Lei Tian, Wilson Wong, Mary J. Dunlop, Ji-Xin Cheng
Summary: The study presents a system for acquiring high-resolution SRS spectra using a polygon scanner, while significantly improving signal-to-noise ratio through the use of a spatial-spectral residual learning network. The authors demonstrate high-speed imaging and tracking of multiple biomolecules in the fingerprint region using a polygon-based ultrafast delay scanner and deep learning framework.
NATURE COMMUNICATIONS
(2021)
Review
Plant Sciences
Mohd Fadhli Hamdan, Shiu-Cheung Lung, Ze-Hua Guo, Mee-Len Chye
Summary: Acyl-CoA-binding proteins (ACBPs) play important roles in reproduction in plants and other organisms, potentially impacting crop improvement in agriculture.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Multidisciplinary Sciences
Lauana Pereira de Oliveira, Bruno Viana Navarro, Joao Pedro de Jesus Pereira, Adriana Rios Lopes, Marina C. M. Martins, Diego Mauricio Riano-Pachon, Marcos Silveira Buckeridge
Summary: This study comprehensively analyzed the sequences and expression levels of enzymes involved in trehalose metabolism in sugarcane, and specifically investigated the post-translational modifications and residues involved in catalysis and substrate binding of trehalose-6-phosphate synthase. The findings revealed the presence of multiple genes related to trehalose metabolism in sugarcane, and identified differences in enzyme activity between TPS Class I and TPS Class II.
SCIENTIFIC REPORTS
(2022)
Article
Agronomy
Zhanyu Chen, Yushuang Wang, Yanbo Chen, Xiaoqin Yang, Shuang Wang, Tingting Yu, Ying Zhou, Xiyan Cui
Summary: This study characterized the molecular properties of GmPLMT and its effects on lipid metabolites in soybeans. The expression patterns of GmPLMT varied in different growth stages of soybeans and transgenic Arabidopsis thaliana seeds, affecting the production of lipid metabolites. Increased expression of GmPLMT was positively correlated with higher levels of PtdCho and crude fats, suggesting its potential for enhancing lipid metabolite production in soybeans.
Article
Environmental Sciences
Shuang Ding, Wen Qi, Qi Xu, Tianyang Zhao, Xu Li, Jianli Yin, Ruxuan Zhang, Chuanyi Huo, Liting Zhou, Lin Ye
Summary: The study revealed that DEHP exposure may affect lipid metabolism in adolescents by regulating the expression of lipid metabolism-related genes, particularly showing significant associations with compounds like MECPP, MCMHP, and n-ary DEHP levels.
ENVIRONMENTAL POLLUTION
(2021)
Article
Plant Sciences
Sujeong Je, Yuree Lee, Yasuyo Yamaoka
Summary: This study examined the effects of seven chemicals that induce endoplasmic reticulum (ER) stress in animals on the growth and gene expression of Chlamydomonas reinhardtii and Arabidopsis thaliana. The results showed that four of these compounds induced ER stress in both organisms and influenced lipid biosynthesis.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Chemistry, Analytical
Ying Xu, Xiaoli Hou, Qiaoqiao Zhu, Shijie Mao, Jie Ren, Jidong Lin, Ning Xu
Summary: A noninvasive, label-free, single-cell Raman imaging analysis platform was developed to distinguish between different cell phenotypes based on their intrinsic biochemical profiles; significant differences were found between HeLa cell WT and CDK6 gene knockout types through Raman spectral analysis; insights into the biological mechanisms of cell phenotypes were revealed by studying interactions between organelles.
ANALYTICAL CHEMISTRY
(2022)
Article
Plant Sciences
Nabieu Kamara, Yamin Jiao, Weicong Huang, Lichong Cao, Lianjun Zhu, Chongchong Zhao, Xu Huang, Fimanekeni Ndaitavela Shivute, Xiangdong Liu, Jinwen Wu, Muhammad Qasim Shahid
Summary: This study aims to investigate the genetic defects related to pollen development and infertility in the novel tetraploid rice germplasm NY2, known as Neo-tetraploid rice. Combined cytological and transcriptome analysis revealed that NY2 gene regulates abnormal tapetal cells, microspore, and middle layer development, leading to pollen abortion and male sterility. Down-regulated transcription factor genes related to pollen development were also identified. This study provides important understanding of the molecular processes underlying fertility control in tetraploid rice.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Microbiology
Roz Laing, Stephen R. Doyle, Jennifer McIntyre, Kirsty Maitland, Alison Morrison, David J. Bartley, Ray Kaplan, Umer Chaudhry, Neil Sargison, Andy Tait, James A. Cotton, Collette Britton, Eileen Devaney
Summary: Through crossbreeding and gene expression analysis, we have identified certain genes associated with ivermectin resistance and proposed a novel mechanism that allows resistant worms to survive treatment. This is crucial for understanding ivermectin resistance and response to treatment in parasitic helminths.
Article
Plant Sciences
Yan Peng, Hongxiang Lou, Zengdong Tan, Zhewen Ouyang, Yuting Zhang, Shaoping Lu, Liang Guo, Bao Yang
Summary: This study investigated the changes in lipidome and metabolome in rapeseed under nitrogen starvation. It was found that nitrogen deficiency affected plant growth and amino acid levels, and also altered membrane lipid metabolism and carbon metabolism. This research provides valuable information for further understanding the metabolic response of rapeseed to nitrogen deficiency.
PLANT AND CELL PHYSIOLOGY
(2023)