Article
Polymer Science
Shumin Liang, Lidong Zhang
Summary: A novel concept of developing a fluorophore-free organogel that can exhibit blue fluorescence in a transparent state and undergo optical switching from blue to purple in a non-transparent state upon phase transition is proposed. The organogel, synthesized by co-crosslinking hydrophilic acrylamide and hydrophobic hexafluorobutyl acrylate using ammonium persulphate initiator in dimethyl sulphoxide, exhibits blue fluorescence generated by the decomposition of ammonium bisulfate during heating. The organogel also demonstrates patternable and reversible optical switching under UV light, making it applicable for information encoding/encryption and optical-switchable soft actuators.
MACROMOLECULAR RAPID COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Laurell F. F. Kessler, Ashwin Balakrishnan, Nina S. S. Deussner-Helfmann, Yunqing Li, Maximilian Mantel, Marius Glogger, Hans-Dieter Barth, Marina S. S. Dietz, Mike Heilemann
Summary: Super-resolution techniques such as SMLM and STED microscopy are enhanced by using weak affinity-based transient labelling systems, such as DNA-based hybrid systems. However, these techniques suffer from high background fluorescence signal caused by unbound fluorophore-labelled oligonucleotides. This study introduces short-distance self-quenching in fluorophore dimers as an efficient mechanism to reduce background fluorescence signal and increase photon budget in the bound state.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Analytical
Xinming Zhang, Chao Jiang, Ting He, Feng Zhao, Junle Qu, Peng Huang, Jing Lin
Summary: In this study, a series of dual imaging probes were successfully synthesized using probe-active pocket match engineering for detecting HNE activity. Among them, probe 2 (LET-8) showed the best selectivity and response ability, and could be used for NIRF/PA dual imaging of HNE activity.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Fabio de Moliner, Zuzanna Konieczna, Lorena Mendive-Tapia, Rebecca S. Saleeb, Katie Morris, Juan Antonio Gonzalez-Vera, Takeshi Kaizuka, Seth G. N. Grant, Mathew H. Horrocks, Marc Vendrell
Summary: The multiple applications of super-resolution microscopy have led to the need for minimally invasive labeling strategies for peptide-guided fluorescence imaging. In this study, we have built a library of benzodiazole amino acids and examined their potential as background-free fluorescence microscopy probes. The results show that benzodiazole amino acids retain the binding capabilities of bioactive peptides and exhibit excellent signal-to-background ratios.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Karolina Maleckaite, Jelena Dodonova, Stepas Toliautas, Rugile Zilenaite, Dziugas Jurgutis, Vitalijus Karabanovas, Sigitas Tumkevicius, Aurimas Vysniauskas
Summary: Viscosity imaging at a microscopic scale is important for studying biosystems, and developing red-emitting, viscosity-sensitive fluorophores is a challenging task. Successful prediction of the viscosity sensitivity of fluorophores through DFT calculations aided in the design of a new red viscosity probe.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Connor W. W. Barth, Syed Zaki Husain Rizvi, Anas M. M. Masillati, Samrat Chakraborty, Lei G. G. Wang, Antonio R. R. Montano, Dani A. A. Szafran, William S. S. Greer, Nynke S. S. van den Berg, Jonathan Sorger, Deepa A. A. Rao, Adam W. G. Alani, Summer L. L. Gibbs
Summary: Patients undergoing gynecological procedures often suffer from long-term side effects caused by nerve damage. A near-infrared fluorescent contrast agent, LGW08-35, has been developed for real-time visualization of important nerves during nerve-sparing gynecologic surgeries, which can help eliminate incontinence and sexual dysfunction in patients.
Article
Physics, Multidisciplinary
Konstantin Grygoryev, Huihui Lu, Celina L. Li, Alexander Zhdanov, Ray Burke, Stefan Andersson-Engels
Summary: This study introduces a pulsed, multi-wavelength system designed to measure diffuse reflectance and auto-fluorescence under strong ambient illumination conditions. Tested on a variety of tissue phantoms and ex-vivo samples, the system shows promise for accurate resection of malignant tumor margins.
FRONTIERS IN PHYSICS
(2021)
Article
Chemistry, Analytical
Junhao Wang, Yuanyuan Liu, Tingting Zhao, Jiaheng Shi, Jing Chen, Dan Li, Yanyun Cui, Shenghao Xu, Xiliang Luo
Summary: Strategies for tumor-specific molecular imaging based on signal amplification have potential in evaluating tumor metastasis and progression. However, traditional amplification strategies are limited by off-tumor signal leakage. In this study, an enzyme-activated autonomous-motion DNAzyme signal amplification strategy (E-DNAzyme) was designed for improved spatial specificity in tumor-specific molecular imaging, which can be specifically activated by overexpressed APE1 in tumor cells. The proposed E-DNAzyme showed a lower detection limit and a higher discrimination ratio compared to traditional amplification strategies.
ANALYTICAL CHEMISTRY
(2023)
Article
Nanoscience & Nanotechnology
Dorina F. Sava Gallis, Kimberly S. Butler, Charles J. Pearce, Nichole Valdez, Mark A. Rodriguez
Summary: This study presents a rapid and facile design strategy to create a highly complex optical tag with programmable, multimodal photoluminescent properties using intrinsic and DNA-fluorophore hidden signatures. A novel heterometallic near-infrared (NIR)-emitting metal-organic framework (MOF) was designed and synthesized as the first covert feature of the tag. The NIR-emissive MOF was post-synthetically modified via a fluorescent DNA oligo labeling design strategy to achieve controlled, multimodal, and tailorable emission with difficult to counterfeit features.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Nanoscience & Nanotechnology
Dorina F. Sava Gallis, Kimberly S. Butler, Charles J. Pearce, Nichole Valdez, Mark A. Rodriguez
Summary: This article introduces a design strategy for creating highly complex optical tags using DNA-fluorophore hidden signatures and near-infrared emissive mesoporous metal-organic frameworks, allowing for controlled, multimodal emission with difficult to counterfeit features. It demonstrates important covert elements and innovative technology.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Rui Zhang, Xuan He, Jia-Min Jiang, Pan-Pan Li, Hai-Yan Wang, Lin Li, Jia-Xiang Yang, Lin Kong
Summary: TD-DFT quantum calculation was performed to predict the electronic transition and absorption/emission maxima of pyrrole-difluoroboron derivatives with different electron donor-acceptor units or conjugated degrees. It led to the design of a new NIR fluorophore TPBD-BP, which showed excellent water solubility, biocompatibility, and AIE for in vivo imaging. The NIR-II emission signal of TPBD-BP dots could be observed in the reproductive organs of normal nude mice, indicating potential applications in biological imaging.
BIOORGANIC CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Enquan Jin, Qiqi Yang, Cheng-Wei Ju, Qiang Chen, Katharina Landfester, Mischa Bonn, Klaus Muellen, Xiaomin Liu, Akimitsu Narita
Summary: This study presents a novel fluorescent probe N-DBOV, which exhibits excellent optical properties and pH sensitivity, making it a promising candidate for fluorescence sensing in biology and environmental analytics.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Multidisciplinary
Michael Weber, Taukeer A. Khan, Lukas J. Patalag, Mariano Bossi, Marcel Leutenegger, Vladimir N. Belov, Stefan W. Hell
Summary: The study introduces ONB-2SiR, a photoactivatable fluorophore that can be activated in UV and specifically de-excited by STED at 775 nm. Additionally, a conjugation and purification protocol is presented to effectively label primary and secondary antibodies with moderately water-soluble dyes, reducing dye aggregation and improving imaging performance.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Chemistry, Analytical
Yu Hou, Dianbing Wang, Song Lu, Dongge Guo, Min Li, Mengmeng Cui, Xian-En Zhang
Summary: A strategy combining the CRISPRSunTag system with a light-inducible nuclear export tag (LEXY) is proposed to reduce background fluorescence and improve visualization of low-repetitive genomic loci, achieving a significant increase in the signal-to-noise ratio.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Jonathan T. Husband, Yujie Xie, Thomas R. Wilks, Louise Male, Miquel Torrent-Sucarrat, Vasilios G. Stavros, Rachel K. O'Reilly
Summary: A new fluorescent dye with rigidochromic behavior was reported, showing high fluorescence in solid matrix and negligible fluorescence in solution. The dye's quantum yield was found to be sensitive to the nature of the surrounding matrix, due to precise tuning of noncovalent interactions at the molecular level. This work expands the diversity of molecules exhibiting solid state environment sensitivity and provides important insights for their design.
