Article
Oncology
Angela M. Carter, Nilesh Kumar, Brendon Herring, Chunfeng Tan, Rachael Guenter, Rahul Telange, Wayne Howse, Fabrice Viol, Tyler R. McCaw, Hayden H. Bickerton, Priyanka Gupta, Frank Gillardon, Eugene A. Woltering, Deepti Dhall, John Totenhagen, Ronadip R. Banerjee, Elizabeth M. Kurian, Sushanth Reddy, Herbert Chen, Joerg Schrader, J. Bart Rose, M. Shahid Mukhtar, James A. Bibb
Summary: Pancreatic neuroendocrine tumors (PanNETs) are a type of tumor that arises from hormone-secreting islet cells of the pancreas. A new inducible, conditional mouse model of PanNETs has been developed, which will facilitate molecular delineation of Cdk5-dependent PanNETs and the development of new targeted therapeutics.
Article
Neurosciences
Israt Jahan, Ryota Adachi, Ryo Egawa, Haruka Nomura, Hiroshi Kuba
Summary: This study reveals that microtubule reorganization via activation of CDK5 plays a role in the plasticity of the axon initial segment (AIS). Treatment with a high-K+ medium shortened the AIS, specifically in neurons tuned to high-frequency sound. CDK5/p35-mediated AIS shortening is achieved by promoting disassembly of microtubules at distal AIS.
JOURNAL OF NEUROSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Chia-Herng Yue, Muhammet Oner, Chih-Yuan Chiu, Mei-Chih Chen, Chieh-Lin Teng, Hsin-Yi Wang, Jer-Tsong Hsieh, Chih-Ho Lai, Ho Lin
Summary: In our study, we investigated the RET activation and its biochemical interaction with CDK5 in GDNF-induced medullary thyroid cancer proliferation. Additionally, we identified the physical interaction between CDK5 and RET protein in MTC for the first time.Overall, our results suggest that targeting CDK5 may be a promising therapeutic candidate for human medullary thyroid cancer in the near future.
Review
Immunology
Chaoyu Ma, Nu Zhang
Summary: This study summarizes recent findings on Tcf-1(+) T cells, focusing on their migratory and resident behavior, as well as the mechanisms underlying their differentiation and maintenance during persistent antigen stimulation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Susanne Krammer, Zuqin Yang, Hannah Mitlaender, Janina C. Grund, Sonja Trump, Susanne Mittler, Sabine Zirlik, Susetta Finotto
Summary: This study found that asthmatic patients had increased expression of TGF-beta RII in peripheral blood mononuclear cells and a suppressed release of TGF-beta after rhinovirus infection. Rhinovirus infection also activated Treg and NK cells.
Article
Biochemistry & Molecular Biology
Toru Tanaka, Sachiyo Ohashi, Akihiko Takashima, Shunsuke Kobayashi
Summary: This study investigates the mechanism of tau protein phosphorylation under neural excitation conditions, and reveals that the translation and hyper-activation of CDK5 are mediated by dendrite-distributed mRNA in response to glutamatergic stimulation.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2022)
Article
Immunology
Sarah K. Whitley, Mushi Li, Sakeen W. Kashem, Toshiro Hirai, Botond Z. Igyarto, Kelley Knizner, Jonhan Ho, Laura K. Ferris, Casey T. Weaver, Daniel J. Cuas, Mandy J. McGeachy, Daniel H. Kaplan
Summary: This study demonstrates the importance of locally produced IL-23 in promoting in situ proliferation of cutaneous TRM17 cells, supporting their longevity and function. Anti-IL-23 therapy effectively reduces the number of TRM17 cells in the skin, which has significant implications for the treatment of psoriasis.
SCIENCE IMMUNOLOGY
(2022)
Article
Plant Sciences
Zhenyu Liu, Weili Wang, Xueqin Li, Sha Tang, Dongwei Meng, Wenli Xia, Hong Wang, Yuzhang Wu, Xinyuan Zhou, Jingbo Zhang
Summary: Capsaicin can effectively alleviate renal fibrosis, possibly by suppressing the activation of myofibroblasts and protecting against the phenotypic alteration of tubular epithelial cells. This is mainly achieved through the inhibition of the TGF-beta 1-Smad2/3 signaling pathway.
Article
Multidisciplinary Sciences
Fei Lu, Shuran Chen, Weijun Shi, Xu Su, Huazhang Wu, Mulin Liu
Summary: The study found that CRC patients with high GPC1 expression had poor overall survival, and GPC1 may promote proliferation and migration of CRC cells by influencing the TGF-beta 1 signaling pathway. Additionally, silencing GPC1 inhibited TGF-beta 1 levels and increased SMAD2 expression.
Article
Cardiac & Cardiovascular Systems
Hideki Amano, Koji Eshima, Yoshiya Ito, Masaki Nakamura, Hidero Kitasato, Fumihiro Ogawa, Kanako Hosono, Kazuya Iwabuchi, Satoshi Uematsu, Shizuo Akira, Shuh Narumiya, Masataka Majima
Summary: mPGES-1/PGE(2) induces neovascularization from ischemia via EP4 by promoting the accumulation of Tregs.
CARDIOVASCULAR RESEARCH
(2023)
Article
Multidisciplinary Sciences
Alan Umfress, Ayanabha Chakraborti, Suma Priya Sudarsana Devi, Raegan Adams, Daniel Epstein, Adriana Massicano, Anna Sorace, Sarbjit Singh, M. Iqbal Hossian, Shaida A. Andrabi, David K. Crossman, Nilesh Kumar, M. Shahid Mukhtar, Huiyang Luo, Claire Simpson, Kathryn Abell, Matthew Stokes, Thorsten Wiederhold, Charles Rosen, Hongbing Lu, Amarnath Natarajan, James A. Bibb
Summary: Millions of traumatic brain injuries (TBIs) occur annually, commonly resulting from falls, traffic accidents, and sports-related injuries involving rotational acceleration/deceleration of the brain. This study developed a new model of repetitive rotational head trauma in rodents and demonstrated acute and prolonged pathological, behavioral, and electrophysiological effects of rotational TBI (rTBI). The aberrant activity of Cyclin-dependent kinase 5 (Cdk5) was identified as a principal mediator of rTBI, and pharmacological inhibition of Cdk5 showed promising results in reducing the cognitive and pathological consequences of injury.
SCIENTIFIC REPORTS
(2023)
Article
Neurosciences
Andrew Cho, Bradford E. Hall, Advait S. Limaye, Sheng Wang, Man-Kyo Chung, Ashok B. Kulkarni
Summary: This study found that modulating pain perception through Cdk5 phosphorylation of TRPV1, knocking in the T407A point mutation had an impact on pain behaviors in mice.
Article
Multidisciplinary Sciences
Tiago C. Luis, Nikolaos Barkas, Joana Carrelha, Alice Giustacchini, Stefania Mazzi, Ruggiero Norfo, Bishan Wu, Affaf Aliouat, Jose A. Guerrero, Alba Rodriguez-Meira, Tiphaine Bouriez-Jones, Iain C. Macaulay, Maria Jasztal, Guangheng Zhu, Heyu Ni, Matthew J. Robson, Randy D. Blakely, Adam J. Mead, Claus Nerlov, Cedric Ghevaert, Sten Eirik W. Jacobsen
Summary: In this study, the researchers uncover a feedback mechanism in which IL-1 secreted by activated platelets signals through niche Lepr+ cells to activate HSCs and restore platelet homeostasis.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Antonio Tejera-Munoz, Laura Marquez-Exposito, Lucia Tejedor-Santamaria, Sandra Rayego-Mateos, Macarena Orejudo, Beatriz Suarez-Alvarez, Carlos Lopez-Larrea, Marta Ruiz-Ortega, Raul R. Rodrigues-Diez
Summary: CCN2 plays a role in both fibrotic responses and growth factor-induced oxidative and proinflammatory responses. Its coexistence with TGF-β is necessary for persistent fibrotic response, but the mechanisms involved are not fully understood. CCN2 increases T beta RII expression in VSMCs and maintains TGF-β pathway activation through an EGFR-SMAD dependent manner.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Kuljira Mongkolpobsin, Chanin Sillapachaiyaporn, Sunita Nilkhet, Tewin Tencomnao, Seung Joon Baek
Summary: This study investigated the effects of stigmasterol on glutamate-induced neurotoxicity. The results showed that stigmasterol significantly inhibited neuronal cell death induced by glutamate by reducing ROS production, restoring mitochondrial membrane depolarization, and improving mitophagy aberration. Additionally, conjugating stigmasterol with soluble soybean polysaccharides and chitosan nanoparticles increased its water solubility and enhanced its protective effect against the Cdk5/p35/p25 signaling pathway.
Article
Virology
Michaela Prochazkova, Tibor Fuzik, Danyil Grybchuk, Francesco Luca Falginella, Lucie Podesvova, Vyacheslav Yurchenko, Robert Vacha, Pavel Plevka
Summary: Leishmania parasites carrying Leishmania RNA virus 1 (LRV1) are more likely to cause severe disease, and the structure of the LRV1 capsid provides insights for developing new treatments.
JOURNAL OF VIROLOGY
(2021)
Article
Multidisciplinary Sciences
Karel Skubnik, Lukas Sukenik, David Buchta, Tibor Fuzik, Michaela Prochazkova, Jana Moravcova, Lenka Smerdova, Antonin Pridal, Robert Vacha, Pavel Plevka
Summary: The Iflaviridae family consists of economically important viruses affecting western honeybees. These viruses induce genome release through conformational changes triggered by acidic pH, with different mechanisms for releasing genomes from their capsids.
Article
Physics, Applied
Michaela Prochazkova, Joanna Pawlat, Frantisek Krcma, Zdenka Kozakova
Summary: This study compared the effects of nonthermal plasma treatment on quinine water solutions using two different configurations, and investigated the impact of adding different electrolytes and modifying initial pH on plasma discharge. The results showed that the gliding arc discharge achieved a slightly higher quinine decomposition rate, and that the stability of quinine solutions was influenced by the post-preparation time.
PLASMA PROCESSES AND POLYMERS
(2022)
Review
Neurosciences
Victoria P. San Martin, Anggelo Sazo, Elias Utreras, Gustavo Moraga-Cid, Gonzalo E. Yevenes
Summary: Pharmacological intervention of glycine receptors can restore inhibitory function and exert anti-nociceptive effects on chronic pain. However, the roles of individual subunits of glycine receptors in chronic pain are not well defined.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Anna Simonova, Veronika Romanska, Barbora Benoni, Karel Skubnik, Lenka Smerdova, Michaela Prochazkova, Kristina Spustova, Ondrej Moravcik, Lenka Gahurova, Jan Paces, Pavel Plevka, Hana Cahova
Summary: The Picornavirales include viruses that infect vertebrates, insects, and plants, and their RNA particles contain various methylated nucleosides, primarily in tRNA molecules, which are present in the form of 3' and 5' fragments and are selectively packaged.
Article
Medicine, Research & Experimental
Lipei Shao, Alejandra Pelayo, Rongye Shi, Jinxia Ma, Hui Liu, Yihua Cai, Michaela Prochazkova, Robert P. Somerville, Sandhya R. Panch, Nirali N. Shah, David F. Stroncek, Ping Jin
Summary: This study found that the expression of the PFKFB4 gene and glycolytic pathway activity were increased in CD22 CAR T-cells associated with cytokine release syndrome (CRS). These results were validated in different cases. The study suggests that drugs targeting PFKFB4 and the glycolytic pathway may be beneficial in treating CRS.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Virology
Michaela Prochazkova, Tibor Fuzik, Danyil Grybchuk, Vyacheslav Yurchenko, Pavel Plevka
Summary: This study presents the structure of Leishmania RNA virus 1 (LRV1), revealing that its capsid is built from dimers and exhibits icosahedral symmetry. Additionally, the location of the RNA polymerase within the viral particle varies.
Article
Multidisciplinary Sciences
Marta Siborova, Tibor Fuzik, Michaela Prochazkova, Jiri Novacek, Martin Benesik, Anders S. Nilsson, Pavel Plevka
Summary: The authors demonstrate that E. coli phage SU10 undergoes conformational changes in nozzle proteins and tail fibers after cell binding, forming a nozzle that allows the delivery of phage DNA into bacterial cytoplasm.
NATURE COMMUNICATIONS
(2022)
Article
Biology
Danyil Grybchuk, Michaela Prochazkova, Tibor Fuzik, Aleksandras Konovalovas, Saulius Serva, Vyacheslav Yurchenko, Pavel Plevka
Summary: This study determined the structure and assembly mechanism of the L-BC virus using cryo-electron microscopy. The L-BC capsid has an icosahedral symmetry and the capsid proteins are stabilized by domain swapping. Additionally, some L-BC virus particles were found to be open and lacked one decamer of capsid proteins.
COMMUNICATIONS BIOLOGY
(2022)
Review
Psychology, Multidisciplinary
Yi Huang, Michaela Prochazkova, Jinjin Lu, Abanoub Riad, Petr Macek
Summary: This scoping review examines the relationship between family environmental contributors and adolescents' health-related outcomes using data from the international survey programme, Health Behaviours in School-aged Children (HBSC). The results indicate that family environment independently influences adolescent health, with the majority of research focusing on the impact of family demographic backgrounds. Limited research exists on parental health behaviors and sibling influences. Further studies should explore the mediator/moderator roles of the family and employ data-driven analyses given the extensive variables and data in HBSC.
FRONTIERS IN PSYCHOLOGY
(2022)
Article
Cell & Tissue Engineering
Victoria Ann Remley, Ashley Collins, Sarah Underwood, Jianjian Jin, Yoon Kim, Yihua Cai, Michaela Prochazkova, Larry Moses, Karen M. Byrne, Ping Jin, David F. Stroncek
Summary: The authors compared the use of an automated closed system with a semi-automated platform for depleting red blood cells (RBCs) from bone marrow. They found that the fully automated processing using the Sepax instrument resulted in comparable RBC depletion and total mononuclear cell recovery to the semi-automated process. The Sepax system is shown to be effective in depleting RBCs from bone marrow.
Article
Green & Sustainable Science & Technology
Martin Nad, Vladimir Brummer, Pavel Losak, Vitezslav Masa, Katerina Sukacova, Dominika Tatarova, Marek Pernica, Michaela Prochazkova
Summary: Researchers have developed a vertical photobioreactor using oval-shaped tubes for the cultivation of microalgae to capture CO2 from flue gas. The results showed that under optimal conditions, this novel photobioreactor achieved high biomass productivity and CO2 fixation efficiency. It also absorbed other minor pollutants from the flue gas, providing additional knowledge for algae cultivation using waste gas sources.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Energy & Fuels
Marek Vondra, Jiri Buzik, David Hornak, Michaela Prochazkova, Vaclav Miklas, Michal Tous, Zdenek Jegla, Vitezslav Masa
Summary: Reducing energy costs in Europe is challenging due to price increases. Local renewable energy sources, such as heat from hot springs, can contribute to this effort. This study aims to find a suitable cooling technology for spa resorts by testing a vacuum cooler and a plate heat exchanger. The vacuum cooler demonstrated greater robustness and resistance to fouling.
Article
Geosciences, Multidisciplinary
Michaela Prochazkova, Marek Vochozka, Zdenek Caha
Summary: The article examines the impact of mining companies' lifecycle on staff turnover and the workforce, with a focus on the potential role of age management. It finds that potential failure of these firms significantly affects staff turnover, necessitating the implementation of effective age management strategies. Additionally, the ongoing conflict in Ukraine has a substantial influence on the mining industry and state energy policies.
ACTA MONTANISTICA SLOVACA
(2022)
Meeting Abstract
Cell & Tissue Engineering
H. W. Song, L. Shao, M. Prochazkova, A. Cheuk, P. Jin, D. Stroncek, J. Khan, S. L. Highfill