Article
Dentistry, Oral Surgery & Medicine
William Johnston, Bob T. Rosier, Miguel Carda-Dieguez, Michael Paterson, Paddy Watson, Krystyna Piela, Marilyn Goulding, Gordon Ramage, Divyashri Baranyia, Tsute Chen, Nezar N. Al-Hebshi, Alex Mira, Shauna Culshaw
Summary: This study investigated the early and later changes in subgingival biofilm following periodontal treatment and found that hand and ultrasonic instruments had similar impacts on the composition of the subgingival plaque microbiome. There were marked changes in biofilm composition in the early stages after treatment, but limited evidence of these changes being associated with treatment outcomes.
JOURNAL OF PERIODONTOLOGY
(2023)
Article
Microbiology
Bruno Bueno-Silva, Karyne Rossit Kiausinus, Francisco Jeferson dos Santos Goncalves, Marcus Vinicius Cintra Moreira, Eder Gonzaga de Oliveira, Aldo Brugnera Junior, Magda Feres, Luciene Cristina Figueiredo
Summary: This study evaluated the antimicrobial effect of Desplac (R) product on the subgingival biofilm. The results showed that Desplac (R) reduced bacterial metabolic activity, altered the composition of the biofilm, and inhibited the growth of certain pathogenic bacteria.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Veterinary Sciences
Avika Ruparell, Corrin Wallis, Richard Haydock, Amy Cawthrow, Lucy J. Holcombe
Summary: The study aimed to determine whether canine gingival margin (GM) plaque could be a reliable surrogate for subgingival (SG) plaque in terms of microbial community. Comparing samples from 381 dogs in the USA, China, and Thailand, it was found that GM and SG plaques shared common microbial communities, with some significant taxa-specific differences identified. The research revealed that GM microbiota exhibited lower variability and diversity but reflected a sub-population associated with SG plaque.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Dentistry, Oral Surgery & Medicine
Yong-Wei Fu, Lu Li, Xiao-Qian Wang, Yi Zhou, Li-Fang Zhu, You-Min Mei, Yan Xu
Summary: CYLD participates in periodontal inflammatory responses by negatively regulating LPS-induced NF-kappa B signalling, as evidenced by reduced CYLD expression in inflamed gingival tissue samples compared to healthy tissue samples and enhanced proinflammatory cytokine mRNA expression in CYLD-knockdown HGFs stimulated with LPS.
Article
Dentistry, Oral Surgery & Medicine
Nouf Alshibani, Reem AlKattan, Eman Allam, Fahad A. Alshehri, Manal Matouq Shalabi, Nada Almuhanna, Houriah Almarshad, Anfal Aljamili
Summary: This study investigated the effects of metformin (MF) on the production of matrix metalloproteinases (MMPs) and proinflammatory cytokines in lipopolysaccharide (LPS) - stimulated human gingival fibroblasts (HGFs). Different concentrations of MF were tested on HGFs, and expression levels of MMP-1, MMP-2, MMP-8, MMP-9, IL-1 beta, and IL-8 were analyzed. The results showed that MF had a minimal cytotoxic effect on HGFs and significantly reduced the expression of MMP-1, MMP-2, MMP-8, and IL-8 in LPS-stimulated HGFs.
Article
Microbiology
Bongsoo Park, Hyunwook Koh, Michael Patatanian, Hermes Reyes-Caballero, Ni Zhao, Jill Meinert, Janet T. Holbrook, Leah I. Leinbach, Shyam Biswal
Summary: This study reports the association between electronic cigarette (EC) use and gingival inflammation, and identifies the mechanism underlying this relationship through mediation analysis of the oral microbiome. The results show significant differences in the microbiome of EC users and the relative abundance of specific microbes mediates the effects of EC use on gingival inflammation. The findings emphasize the need for continued surveillance on the impact of ECs on the oral microbiome and oral diseases.
Article
Biochemistry & Molecular Biology
Miquel Antich-Rossello, Marta Munar-Bestard, Maria Antonia Forteza-Genestra, Javier Calvo, Antoni Gaya, Marta Monjo, Joana M. Ramis
Summary: This study investigated the combination of extracellular vesicles (EVs) derived from platelet lysate (PL) and hyaluronic acid biomaterials (HA) in an in vitro wound healing assay. The results showed that the combination of EVs and HA had good biocompatibility in gingival fibroblasts and keratinocytes, and could enhance wound healing in vitro. Furthermore, changes in gene expression related to extracellular matrix remodeling were observed after treatment with EVs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Aya Q. Alali, Abdalla Abdal-hay, Karan Gulati, Saso Ivanovski, Benjamin P. J. Fournier, Ryan S. B. Lee
Summary: This study investigated the effect of bioinspired lithium-doped titanium surfaces on human gingival fibroblasts and oral biofilm. Results showed that Li_Ti and NaOH_Ti surfaces promoted fibroblast proliferation, increased FGF-2 mRNA levels, and decreased bacterial metabolic activity. The bioinspired Li-doped Ti surfaces were found to enhance HGF bioactivity while inhibiting bacterial adhesion and growth, which is clinically significant for improving soft tissue integration during dental implant treatment maintenance.
Article
Dentistry, Oral Surgery & Medicine
Kubra Aral, Michael R. Milward, Paul R. Cooper
Summary: This study aimed to investigate the potential regulatory pathways of IL-1 beta production by monitoring the effects of periodontal pathogens Fn and Pg on inflammasomes and their regulators in HGFs. The results showed that Fn and Pg, especially when combined with ATP exposure, could dysregulate NLRP3, AIM2, IL-1 beta, ASC, and other inflammasome components and regulators, ultimately affecting the production of IL-1 beta.
Article
Pharmacology & Pharmacy
Ying Zhang, Yanyang Guo, Wenjia Wei, Zhongxiao Zhang, Xiaodong Xu
Summary: This article examines the biological effects and molecular mechanisms of berberine in an inflammatory response model in human gingival fibroblasts induced by lipopolysaccharide (LPS) using metabolomics. The study found that berberine can suppress the secretion of inflammatory cytokines and apoptosis in HGFs, suggesting its potential as a therapeutic drug for periodontitis.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Soon Chul Heo, Yu Na Kim, Bo Ram Keum, Ji-Young Joo, Moon-Kyoung Bae, Hyung Joon Kim
Summary: This study investigates the role of VASH1 in periodontal disease and reveals its importance in promoting osteoclastogenesis through inducing RANKL expression in human gingival fibroblasts. These findings provide insights into the pathological mechanism of periodontal disease and offer potential targets for therapeutic intervention.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2023)
Article
Dentistry, Oral Surgery & Medicine
Jamil Awad Shibli, Thayane Furtado Rocha, Fernanda Coelho, Ticiana Sidorenko de Oliveira Capote, Sybele Saska, Marcelo A. Melo, Joao Marcos Spessoto Pingueiro, Marcelo de Faveri, Bruno Bueno-Silva
Summary: This study evaluated the metabolic activity of HCOBc and CHX on subgingival biofilms, showing a significant reduction in biofilm metabolic activity and specific bacterial species with both treatments. Both substances exhibited similar cytotoxicity results. HCOBc was better than CHX in reducing red-complex bacterial proportions.
CLINICAL ORAL INVESTIGATIONS
(2021)
Article
Biochemistry & Molecular Biology
Diana Lorena Guevara Solarte, Sibylle Johanna Rau, Elmar Hellwig, Kirstin Vach, Ali Al-Ahmad
Summary: This study aimed to evaluate the antimicrobial properties and cytotoxicity of antimicrobial photodynamic therapy (aPDT) using indocyanine green (ICG) in combination with visible light (Vis) and water-filtered infrared A (wIRA). The results showed that aPDT effectively eliminated most periodontal bacteria and subgingival biofilms, but also exhibited cytotoxicity towards human gingival cells. These findings raise concerns about the potential toxicity of this specific aPDT as an adjuvant to periodontal treatments.
Article
Dentistry, Oral Surgery & Medicine
Ahmed Khocht, Michael Orlich, Bruce Paster, Denise Bellinger, Leticia Lenoir, Crissy Irani, Gary Fraser
Summary: This study found that there were no significant differences in periodontal inflammation indicators and microbial diversity between vegetarians and non-vegetarians. Vegetarians tended to have more bacteria associated with gingival health. IL-10 showed positive correlations with species beneficial for periodontal health in vegetarians.
JOURNAL OF PERIODONTAL RESEARCH
(2021)
Article
Dentistry, Oral Surgery & Medicine
Pasquale Santamaria, Aysegul Sari, Luigi Nibali
Summary: The aim of this study was to assess the differential molecular profiling of gingival crevicular fluid (GCF) from infrabony and suprabony periodontal defects compared with healthy sites. The results showed that 14 biomarkers levels were elevated in GCF from infrabony and suprabony defects compared with healthy sites, while the difference between infrabony and suprabony defects was not significant in biomarker changes.
JOURNAL OF CLINICAL PERIODONTOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Fabio R. M. Leite, Gustavo G. Nascimento, Holger J. Moller, Georgios N. Belibasakis, Nagihan Bostanci, Patricio C. Smith, Rodrigo Lopez
Summary: The study found that fast gingivitis development was associated with higher levels of non-organized response and lower levels of organized response, while slow responders were not associated with gingivitis. The results suggest that individual variability may play a role in susceptibility to gingivitis.
JOURNAL OF CLINICAL PERIODONTOLOGY
(2022)
Article
Chemistry, Physical
Felix J. Geissel, Varvara Platania, Alexander Gogos, Inge K. Herrmann, Georgios N. Belibasakis, Maria Chatzinikolaidou, Georgios A. Sotiriou
Summary: This study demonstrates that the concentration of Ag ions in solution is the major factor driving the antibiofilm effect of nanosilver coatings, independent of Ag size and coating thickness. The antibiofilm effect predominantly stems from the released Ag ions rather than direct bacterial contact inhibition, especially for coatings featuring the smallest Ag particle sizes.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Engineering, Biomedical
Matthew Blakeley, Prashant K. Sharma, Hans J. Kaper, Nagihan Bostanci, Thomas Crouzier
Summary: Disruption of lubrication between oral surfaces due to salivary gland hypofunction can negatively impact quality of life. Research suggests that functionalizing polyethylene glycol with wheat germ agglutinin and immobilizing it on oral epithelial cells and tissue can mimic the salivary film, providing long-lasting relief from mucosal dryness.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Microbiology
Kai Bao, Rolf Claesson, Peter Gehrig, Jonas Grossmann, Jan Oscarsson, Georgios N. Belibasakis
Summary: This study characterized the full proteome of ten strains of F. alocis using label-free quantification proteomics and identified differentially expressed proteins associated with FtxA-positive strains. The study also revealed specific interactions of FtxA with other intracellular proteins, highlighting its role as a major virulence factor of this species.
Review
Microbiology
Preethi Balan, Georgios Belibasakis, Saso Ivanovski, Nagihan Bostanci, Chaminda Jayampath Seneviratne
Summary: The microbial etiology of periodontitis has been extensively studied, but the translation of this knowledge into therapeutic interventions is limited. This review emphasizes the need for innovative clinical interventions beyond conventional therapies and highlights the importance of understanding the structure and function of subgingival microbiota for developing microbiome-based interventions.
CRITICAL REVIEWS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Daniel Manoil, Ana Parga, Cecilia Hellesen, Arwa Khawaji, Malin Brundin, Stephane Durual, Volkan Ozenci, Hong Fang, Georgios N. Belibasakis
Summary: This study aimed to assess the transcriptional responses to sublethal photo-oxidative stress caused by antimicrobial photodynamic therapy (aPDT) in Enterococcus faecalis strains. The results showed that surviving cells up-regulated genes involved in stress survival and virulence, including vancomycin-resistance genes in carrier isolates.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2022)
Article
Microbiology
Kai Bao, Rolf Claesson, Georgios N. Belibasakis, Jan Oscarsson
Summary: This study used label-free quantification proteomics to analyze the expression of extracellular vesicle (EV) proteins in ten strains of F. alocis. While most proteins were consistently identified among the strains, several proteins showed differential expression, suggesting potential variations in virulence potential among EVs from different F. alocis strains.
Review
Dentistry, Oral Surgery & Medicine
Georgios N. Belibasakis, Daniel Belstrom, Sigrun Eick, Ulvi K. Gursoy, Anders Johansson, Eija Koenoenen
Summary: This narrative review provides an overview of the collective knowledge on periodontal microbiology, with a focus on the European contribution in the global field. It discusses the etiological concepts of periodontal disease, particularly the ecological plaque hypothesis and its interpretation. The review also explores anaerobic microbiology, the discovery of periodontal pathogens and their virulence factors, as well as biofilms. Additionally, it highlights the evolution of molecular methods and high-throughput platforms in understanding the periodontal microbiome. Finally, the review examines the role of different microbial species in periodontal diagnosis, the use of microbial and host biomarkers, and the application of antimicrobials in treatment.
PERIODONTOLOGY 2000
(2023)
Article
Chemistry, Multidisciplinary
Felix J. Geissel, Varvara Platania, Niccolo DeBerardinis, Charlotte Skjoldebrand, Georgios N. Belibasakis, Cecilia Persson, Gry Hulsart-Billstrom, Maria Chatzinikolaidou, Georgios A. Sotiriou
Summary: Bone implants often fail due to bacterial colonization and loose biofilm infections. A novel nanoparticle coating composed of silver and bioglass has been developed to address these issues. The coating is produced using a scalable and reproducible process and exhibits both antibacterial and osteogenic properties. The silver-ion release inhibits biofilm formation, while the coating maintains high biocompatibility.
ADVANCED MATERIALS INTERFACES
(2023)
Article
Microbiology
Ana Parga, Daniel Manoil, Malin Brundin, Ana Otero, Georgios N. Belibasakis
Summary: This study assessed the effect of AHLs on biofilm formation and transcriptional regulations in Enterococcus faecalis and found that AHLs promote biofilm formation and up-regulate a transcriptional network involved in virulence and stress tolerance.
JOURNAL OF ORAL MICROBIOLOGY
(2023)
Article
Microbiology
Rolf Claesson, Anders Johansson, Georgios N. Belibasakis
Summary: The aim of this study was to compare the prevalence and proportions of three bacterial species in periodontitis pocket samples from young and old patients. The results showed that the prevalence of Aggregatibacter actinomycetemcomitans was higher in young patients, while Porphyromonas gingivalis was similarly distributed in both age groups. Parvimonas micra was more prevalent in old patients. Furthermore, the proportion of samples containing A. actinomycetemcomitans and P. micra together was higher than when P. gingivalis was present.
Article
Dentistry, Oral Surgery & Medicine
Nil Yakar, Nagihan Bostanci, Gueven Ozdemir, Harika Atmaca, Asena Turedi, Cagdas Sahin, Timur Kose, Gulnur Emingil
Summary: This study compared the levels of cytokines in saliva between postmenopausal and regularly menstruating women, and found that there was no significant difference after adjusting for other factors. Age may be a stronger confounding factor.
JOURNAL OF PERIODONTOLOGY
(2023)
Review
Dentistry, Oral Surgery & Medicine
Nagihan Bostanci, Georgios N. N. Belibasakis
Summary: The interface of molecular science and technology is guiding the transformation of personalized to precision healthcare. The application of proteomics, genomics, transcriptomics, and metabolomics is shaping the suitability of biomarkers for disease. Incorporation of molecular discoveries into routine clinical practice relies on the development of customized assays and devices that enable the rapid delivery of analytical data to the clinician, while the patient is still in session.
CLINICAL ORAL INVESTIGATIONS
(2023)
Review
Dentistry, Oral Surgery & Medicine
Rolf Claesson, Anders Johansson, Georgios N. Belibasakis
Summary: Diagnosis and treatment in dentistry rely on clinical examination, but microbiological analysis can provide supportive evidence to aid decision-making. Clinical microbiology can improve treatment outcomes for conditions such as periodontitis, endodontic infections, and odontogenic abscesses.
FRONTIERS IN ORAL HEALTH
(2022)
Review
Dentistry, Oral Surgery & Medicine
Timo Sorsa, Solomon Olusegun Nwhator, Dimitra Sakellari, Andreas Grigoriadis, Kehinde Adesola Umeizudike, Ella Brandt, Mutlu Keskin, Taina Tervahartiala, Pirjo Parnanen, Shipra Gupta, Ritin Mohindra, Nagihan Bostanci, Nurcan Buduneli, Ismo Tapani Raisanen
Summary: The manuscript highlights the benefits of active-matrix metalloproteinase-8 (aMMP-8) chairside/point-of-care diagnostic tools in oral and systemic diseases. Previous studies support aMMP-8 as a more precise biomarker compared to other markers. The lateral flow aMMP-8 point-of-care testing shows comparable results to protease activity assays and can be applied in various medical fields.
FRONTIERS IN ORAL HEALTH
(2022)