Garbage in, garbage out: A critical evaluation of strategies used for validation of immunohistochemical biomarkers

Antibody performance in western blot applications is context-dependent

(2014) Cajsa Älgenäs et al.   Biotechnology Journal

The human liver-specific proteome defined by transcriptomics and antibody-based profiling

(2014) Caroline Kampf et al.   FASEB JOURNAL

Antibody validation of immunohistochemistry for biomarker discovery: Recommendations of a consortium of academic and pharmaceutical based histopathology researchers

(2014) William J. Howat et al.   METHODS

A systematic analysis of commonly used antibodies in cancer diagnostics

(2013) Gabriela Gremel et al.   HISTOPATHOLOGY

A matrix approach to guide IHC-based tissue biomarker development in oncology drug discovery

(2013) Neil R Smith et al.   JOURNAL OF PATHOLOGY

Analysis of the Human Tissue-specific Expression by Genome-wide Integration of Transcriptomics and Antibody-based Proteomics

(2013) Linn Fagerberg et al.   MOLECULAR & CELLULAR PROTEOMICS

Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

(2013) Seung Woo Cho et al.   NATURE BIOTECHNOLOGY

Highly multiplexed single-cell analysis of formalin-fixed, paraffin-embedded cancer tissue

(2013) M. J. Gerdes et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

RNA-Seq Atlas—a reference database for gene expression profiling in normal tissue by next-generation sequencing

(2012) Markus Krupp et al.   BIOINFORMATICS

Mammostrat As an Immunohistochemical Multigene Assay for Prediction of Early Relapse Risk in the Tamoxifen Versus Exemestane Adjuvant Multicenter Trial Pathology Study

(2012) John M.S. Bartlett et al.   JOURNAL OF CLINICAL ONCOLOGY

Utility of multispectral imaging in automated quantitative scoring of immunohistochemistry

(2012) Christopher Fiore et al.   JOURNAL OF CLINICAL PATHOLOGY

Novel pancreatic beta cell-specific proteins: Antibody-based proteomics for identification of new biomarker candidates

(2012) Cecilia Lindskog et al.   Journal of Proteomics

Scalable In Situ Hybridization on Tissue Arrays for Validation of Novel Cancer and Tissue-Specific Biomarkers

(2012) Sara Kiflemariam et al.   PLoS One

Antibodies for profiling the human proteome-The Human Protein Atlas as a resource for cancer research

(2012) Anna Asplund et al.   PROTEOMICS

Prognostic Value of a Combined Estrogen Receptor, Progesterone Receptor, Ki-67, and Human Epidermal Growth Factor Receptor 2 Immunohistochemical Score and Comparison With the Genomic Health Recurrence Score in Early Breast Cancer

(2011) Jack Cuzick et al.   JOURNAL OF CLINICAL ONCOLOGY

Factors Influencing the Degradation of Archival Formalin-Fixed Paraffin-Embedded Tissue Sections

(2011) Ran Xie et al.   JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY

Antigen Retrieval Causes Protein Unfolding

(2011) Carol B. Fowler et al.   JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY

The Human Protein Atlas as a proteomic resource for biomarker discovery

(2011) F. Pontén et al.   JOURNAL OF INTERNAL MEDICINE

The evolution of gene expression levels in mammalian organs

(2011) David Brawand et al.   NATURE

Global quantification of mammalian gene expression control

(2011) Björn Schwanhäusser et al.   NATURE

Phosphoproteins and the Dawn of Functional Phenotyping

(2011) J. Bodo et al.   PATHOBIOLOGY

Clinical and Biological Significance of E-cadherin Protein Expression in Invasive Lobular Carcinoma of the Breast

(2010) Emad A. Rakha et al.   AMERICAN JOURNAL OF SURGICAL PATHOLOGY

Antibody validation

(2010) Jennifer Bordeaux et al.   BIOTECHNIQUES

American Society of Clinical Oncology/College of American Pathologists Guideline Recommendations for Immunohistochemical Testing of Estrogen and Progesterone Receptors in Breast Cancer

(2010) M. Elizabeth H. Hammond et al.   JOURNAL OF CLINICAL ONCOLOGY

Profiling protein expression and interactions: proximity ligation as a tool for personalized medicine

(2010) A. Blokzijl et al.   JOURNAL OF INTERNAL MEDICINE

Towards a knowledge-based Human Protein Atlas

(2010) Mathias Uhlen et al.   NATURE BIOTECHNOLOGY

Prediction of the human membrane proteome

(2010) Linn Fagerberg et al.   PROTEOMICS

siRNA vs. shRNA: Similarities and differences

(2009) Donald D. Rao et al.   ADVANCED DRUG DELIVERY REVIEWS

Bright-Field Microscopy Visualization of Proteins and Protein Complexes by In Situ Proximity Ligation with Peroxidase Detection

(2009) A. Zieba et al.   CLINICAL CHEMISTRY

A global view of protein expression in human cells, tissues, and organs

(2009) Fredrik Pontén et al.   Molecular Systems Biology

BioGPS: an extensible and customizable portal for querying and organizing gene annotation resources

(2009) Chunlei Wu et al.   GENOME BIOLOGY

SPOCTOPUS: a combined predictor of signal peptides and membrane protein topology

(2008) Håkan Viklund et al.   BIOINFORMATICS

Multiple immunofluorescence labelling of formalin-fixed paraffin-embedded (FFPE) tissue

(2008) David Robertson et al.   BMC CELL BIOLOGY

Virtual microscopy as an enabler of automated/quantitative assessment of protein expression in TMAs

(2008) Catherine Conway et al.   HISTOCHEMISTRY AND CELL BIOLOGY

A Guide to the Perplexed on the Specificity of Antibodies

(2008) Clifford B. Saper   JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY

Commercial Antibodies: The Good, Bad, and Really Ugly

(2008) John R. Couchman   JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY

State of the art in antigen retrieval for immunohistochemistry

(2008) Fabio D'Amico et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Hypothesis for the mechanism for heat-induced antigen retrieval occurring on fresh frozen sections without formalin-fixation in immunohistochemistry

(2008) Kochi Kakimoto et al.   JOURNAL OF MOLECULAR HISTOLOGY

Characterizing proteins and their interactions in cells and tissues using the in situ proximity ligation assay

(2008) Ola Söderberg et al.   METHODS

Antibodypedia, a Portal for Sharing Antibody and Antigen Validation Data

(2008) Erik Björling et al.   MOLECULAR & CELLULAR PROTEOMICS

Selected reaction monitoring for quantitative proteomics: a tutorial

(2008) Vinzenz Lange et al.   Molecular Systems Biology

RNA-Seq: a revolutionary tool for transcriptomics

(2008) Zhong Wang et al.   NATURE REVIEWS GENETICS