Review
Cell Biology
Niyaz Mohammadzadeh Honarvar, Meysam Zarezadeh, Samantha A. Molsberry, Alberto Ascherio
Summary: Aging leads to decreased serum levels of phospholipids and sphingomyelins in men, and increased levels in women; however, inconsistencies were found in studies on LPC in men and PC in women.
AGEING RESEARCH REVIEWS
(2021)
Article
Cell Biology
Kuo-Hsuan Chang, Mei-Ling Cheng, Hsiang-Yu Tang, Cheng-Yu Huang, Hsiu-Chuan Wu, Chiung-Mei Chen
Summary: Using targeted metabolomics analysis, this study identified new blood biomarker candidates for Parkinson's disease (PD) and found significant correlations between these biomarkers and disease progression and clinical manifestations of PD.
Review
Nutrition & Dietetics
Ake Nilsson, Rui-Dong Duan, Lena Ohlsson
Summary: Milk polar lipids are essential for tissue growth in infants and may inhibit cholesterol absorption, with beneficial effects on the gut and metabolism. The milk fat globule membrane contains a variety of components that can be enriched through technical procedures to influence digestion and absorption pathways.
FRONTIERS IN NUTRITION
(2021)
Article
Cell Biology
Yu-Qin Di, Xiao-Lin Han, Xin-Le Kang, Di Wang, Cai-Hua Chen, Jin-Xing Wang, Xiao-Fan Zhao
Summary: CTSD has dual functions in promoting cell proliferation and apoptosis, with its secretion determined by N233 glycosylation and expression promoted by 20E hormone. Autophagy triggers its maturation and localization inside cells, leading to activation of CASP3 and apoptosis. Its differential expression in tissues plays a role in determining cell fates during lepidopteran metamorphosis.
Article
Biochemistry & Molecular Biology
Qiangqiang Li, Gang Chen, Dan Zhu, Wenwen Zhang, Suzhen Qi, Xiaofeng Xue, Kai Wang, Liming Wu
Summary: A study found that phosphatidylcholine (PC) has better effects in preventing colitis compared to sphingomyelin (SM), inhibiting colonic tissue lesions and pro-inflammatory cytokines expressions. Furthermore, supplementation of PC and SM can impact gut microbiota abundance and metabolism. These findings provide a scientific foundation for further research in developing more effective dietary intervention strategies.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Xiaosen Ouyang, Willayat Y. Wani, Gloria A. Benavides, Matthew J. Redmann, Hai Vo, Thomas van Groen, Victor M. Darley-Usmar, Jianhua Zhang
Summary: Lysosomes are crucial for protein and organelle degradation and homeostasis, with cathepsins playing a key role in protein quality control. Overexpression of CTSD can reduce the toxicity of protein aggregates and extend the lifespan of certain mutant mice.
ACTA PHARMACEUTICA SINICA B
(2023)
Article
Medicine, General & Internal
Lukasz Oldak, Patrycja Milewska, Sylwia Chludzinska-Kasperuk, Kamil Grubczak, Joanna Reszec, Ewa Gorodkiewicz
Summary: This study focused on quantifying cathepsin B, D, and S in gliomas, exploring their correlation with patient age, tumor size, and each other. The research led to the development of a diagnostic method for distinguishing different grades of glioma and identifying independent markers using cathepsins.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Chemistry, Applied
Jiaxin Wang, Xuening Lu, Jiaen Zhang, Zeheng Xiao
Summary: Lipids including PC, PE, SM, and LPC were extracted from golden apple snails, with SM being the most abundant lipid. The ethanol/hexane extraction method from GAS proved effective and time-saving, showing potential for controlling the snail invasion.
Article
Food Science & Technology
Nahyun Ahn, Jee-Young Imm
Summary: The effects of adding different phospholipid (PL) matrices on emulsion stability, microstructure, and in vitro simulated lipid digestion were studied. The addition of milk sphingomyelin (SM) significantly increased emulsion stability and free fatty acid release during digestion compared to soy phosphatidylcholine (PC). This was attributed to reduced droplet aggregation and improved digestibility. The incorporation of SM also delayed the digestion of beta-lactoglobulin. Thus, the selection of appropriate emulsifiers can enhance lipid digestion in infants and have nutritional benefits.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Virology
Yuexuan Wang, Honglin Han, Kecheng Zhu, Suifeng Xu, Chengzong Han, Yunxiang Jiang, Shina Wei, Qiwei Qin
Summary: In this study, Ec-Cathepsin D (Ec-CD) was identified from the orange-spotted grouper and its function in SGIV infection was investigated. The overexpression of Ec-CD inhibited SGIV replication, SGIV-induced apoptosis, and pro-inflammatory cytokine production. It also suppressed the activation of MAPK pathways.
Article
Endocrinology & Metabolism
Jingzhe Song, Nan Zhu, Xinchen Pan, Lu Guo, Xiang Kong
Summary: This study investigated the expression and significance of Cathepsin C (Cat C) and Cathepsin D (Cat D) in the serum and placenta of nonpregnant women, pregnant women in different trimesters, and patients with preeclampsia (PE). The results showed that the levels of Cat C were significantly lower in the first trimester compared to the nonpregnant group, while Cat D levels were significantly higher. Cat C and D levels increased in the second and third trimesters, with no significant difference between the two trimesters. In patients with PE, Cat C levels were significantly higher and Cat D levels were significantly lower than in the third trimester, and these levels were correlated with the severity of PE.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Kuo-Hsuan Chang, Chia-Ni Lin, Chiung-Mei Chen, Rong-Kuo Lyu, Chun-Che Chu, Ming-Feng Liao, Chin-Chang Huang, Hong-Shiu Chang, Long-Sun Ro, Hung-Chou Kuo
Summary: This study identified a panel of metabolites correlated with disease severity in ALS, some of which show potential as biomarkers for monitoring disease progression and therapeutic effects. Machine learning algorithms using selected metabolites achieved good performance in distinguishing ALS patients from normal controls.
Article
Biochemistry & Molecular Biology
Daniel Janitschke, Anna Andrea Lauer, Cornel Manuel Bachmann, Jakob Winkler, Lea Victoria Griebsch, Sabrina Melanie Pilz, Elena Leoni Theiss, Heike Sabine Grimm, Tobias Hartmann, Marcus Otto Walter Grimm
Summary: Alzheimer's disease is characterized by increased plaque formation and tangle accumulation in the brain, along with extensive lipid alterations. Methylxanthines, a type of alkaloid commonly consumed through diet, have been found to interfere with the molecular mechanisms leading to Alzheimer's disease. Our study shows that methylxanthines not only affect triglycerides and cholesterol in the liver and serum, but also induce changes in other lipid classes in neuroblastoma cells. These changes include both beneficial and adverse effects related to Alzheimer's disease. Therefore, we suggest combining methylxanthines with a diet that alters lipid metabolism to counteract the adverse effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Sandeep Goyal, Ketul V. Patel, Yadav Nagare, Digambar B. Raykar, Santosh S. Raikar, Atul Dolas, Princy Khurana, Rajath Cyriac, Sharad Sarak, Mukesh Gangar, Anil K. Agarwal, Aditya Kulkarni
Summary: This study describes the design, optimization, and evaluation of novel non-peptidic acylguanidine based small molecule inhibitors of Cathepsin D, leading to the identification of an optimized lead 24e with improved microsomal stability and potency. This compound could serve as a potential candidate for further preclinical studies against diseases where Cathepsin D plays a key role.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Zvonimir Boban, Ivan Mardesic, Witold Karol Subczynski, Drazan Jozic, Marija Raguz
Summary: The optimization of GUV production from phosphatidylcholine/sphingomyelin/cholesterol mixtures using the electroformation method requires careful consideration of electrical parameters, lipid film thickness, and cholesterol concentration. Results show that the optimal lipid film thickness is around 30 nm, with the best frequency-voltage combinations in the range of 2-6 V and 10-100 Hz. Increasing cholesterol content leads to a decrease in GUV yield and size, highlighting the importance of balancing lipid composition for high-quality vesicle production in experimental research.
Review
Gastroenterology & Hepatology
Cordelia Schuler, Federica Foti, Leonie Perren, Celine Mamie, Bruce Weder, Michelle Stokmaier, Cheryl de Valliere, Rainer Heuchel, Pedro A. Ruiz, Gerhard Rogler, Martin Hausmann
Summary: This study evaluated the effect of Smad7 deficiency on inflammation and fibrogenesis, and showed that Smad7 deficiency is associated with ameliorated inflammation and increased collagen deposition.
INFLAMMATORY BOWEL DISEASES
(2023)
Article
Cell Biology
Barbora Adamova, Kamila Rihova, Jana Pokludova, Petr Benes, Jan Smarda, Jarmila Navratilova
Summary: An acidic environment and hypoxia in cancer cells contribute to their resistance to therapy. The deregulation of the PI3K/Akt pathway is common in colon cancer. Combining Akt inhibitors with Bcl-2/Bcl-xL inhibitors could be an effective treatment strategy for colon cancer.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2023)
Article
Gastroenterology & Hepatology
Yuhan Yin, Anna Sichler, Josef Ecker, Melanie Laschinger, Gerhard Liebisch, Marcus Hoering, Marijana Basic, Andre Bleich, Xue-Jun Zhang, Ludwig Kuebelsbeck, Johannes Plagge, Emely Scherer, Dirk Wohlleber, Jianye Wang, Yang Wang, Marcella Steffani, Pavel Stupakov, Yasmin Gaertner, Fabian Lohoefer, Carolin Mogler, Helmut Friess, Daniel Hartmann, Bernhard Holzmann, Norbert Hueser, Klaus-Peter Janssen
Summary: Hepatocyte growth and proliferation depend on membrane phospholipid biosynthesis, which is significantly influenced by short-chain fatty acids (SCFAs) generated through bacterial fermentation. Antibiotic treatment and dysbiosis not only affect gut microbiota, but also impair hepatic lipid synthesis and liver regeneration.
JOURNAL OF HEPATOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Lea Schaefer, Sarah M. Grundmann, Silvia Friedrichs, Dieter Luetjohann, Marcus Hoering, Gerhard Liebisch, Erika Most, Robert Ringseis, Klaus Eder
Summary: Replacing soybean oil with Hermetia illucen larvae fat alters the fatty acid composition of meat and reduces lipid oxidation.
ARCHIVES OF ANIMAL NUTRITION
(2023)
Article
Hematology
Lilli Arndt, Ileana Hernandez-Resendiz, Doreen Moos, Janine Dokas, Silvana Mueller, Franziska Jeromin, Richard Wagner, Uta Ceglarek, Iris M. Heid, Marcus Hoering, Gerhard Liebisch, Sonja C. Stadler, Ralph Burkhardt
Summary: This study demonstrates that deficiency of Trib1 promotes the formation of atherosclerotic lesions and increases plasma cholesterol and triglyceride levels in Ldlr(-/-) mice. It also suggests that Trib1 deficiency alters hepatic lipid metabolism and induces inflammation.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Article
Genetics & Heredity
Lisa Rein-Fischboeck, Rebekka Pohl, Elisabeth M. Haberl, Wolfgang Mages, Philipp Girke, Gerhard Liebisch, Sabrina Krautbauer, Christa Buechler
Summary: Visceral adiposity is associated with liver steatosis and the development of NASH. Mice lacking SNTB2 have reduced intra-abdominal fat mass and do not accumulate hepatic cholesteryl esters on a MCD diet. However, they still develop a similar NASH pathology as wild type mice, suggesting a minor role of intra-abdominal fat and liver cholesteryl esters in this model.
Article
Biochemistry & Molecular Biology
Georg Peschel, Kilian Weigand, Jonathan Grimm, Martina Mueller, Sabrina Krautbauer, Marcus Hoering, Gerhard Liebisch, Christa Buechler
Summary: This study analyzed the composition of sphingomyelin (SM) in the serum of patients with chronic hepatitis C virus (HCV) infection, and found differences in SM species among different genders, genotypes, and patients with liver cirrhosis. The levels of SM were more closely associated with liver function in male patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Qingqing Wang, Miriam Hoene, Chunxiu Hu, Louise Fritsche, Robert Ahrends, Gerhard Liebisch, Kim Ekroos, Andreas Fritsche, Andreas L. Birkenfeld, Xinyu Liu, Xinjie Zhao, Qi Li, Benzhe Su, Andreas Peter, Guowang Xu, Rainer Lehmann
Summary: Reliability, robustness, and interlaboratory comparability of quantitative measurements in clinical lipidomics studies are crucial. The different ex vivo stability of lipids in blood poses a risk to data interpretation. Clear recommendations for blood sample collection are necessary. In this study, we used UHPLC-high resolution mass spectrometry to investigate the stability of 417 lipid species in EDTA whole blood under different temperature conditions and time points. Our data suggest that cooling whole blood immediately and continuously, and separating plasma within 4 hours, unless focusing on robust lipids, are recommended. Lists of lipids' ex vivo (in)stability and potential biomarkers in whole blood are provided. Overall, our results contribute to the international efforts in achieving reliable and comparable clinical lipidomics studies in the future.
JOURNAL OF LIPID RESEARCH
(2023)
Article
Biochemical Research Methods
Petr Lapcik, Barbora Vesela, David Potesil, Katerina Dadakova, Martina Zapletalova, Petr Benes, Pavel Bouchal, Eva Matalova
Summary: Inhibition of caspase-9 leads to changes in the proteome of chondrogenic cultures, with alterations in extracellular matrix organization, collagen metabolism, and muscle system processes. Cells shifted from glycolytic to lipid-based metabolism, suggesting a non-apoptotic role of caspases in chondrocyte homeostasis.
Article
Biochemistry & Molecular Biology
Coenraad Frederik Slabber, Marc Bachofner, Tobias Speicher, Andrii Kuklin, Abbie Elisabeth Fearon, Susagna Padrissa-Altes, Roman Bogorad, Carla Horvath Rudigier, Daria Wust, Sabrina Krautbauer, Marcus Hoering, Gerhard Liebisch, Daniel G. Anderson, Christian Wolfrum, Ulrich Auf Dem Keller, Sabine Werner
Summary: Mice lacking FGF receptors 1 and 2 (Fgfr1 and Fgfr2) in hepatocytes are hypersensitive to cytotoxic injury during liver regeneration, which is associated with the protective role of the urinary-reproductive factor Uhrf2 in liver cells. Uhrf2 expression increases during regeneration after partial hepatectomy in an FGFR-dependent manner, but the abundance of Uhrf2 in liver cells is lower in FGFR-deficient mice. Hepatocyte-specific knockout of Uhrf2 leads to liver necrosis, impaired hepatocyte proliferation, and liver failure.
Article
Multidisciplinary Sciences
Susanne Heimerl, Marcus Hoering, Dominik Kopczynski, Alexander Sigruener, Christina Hart, Ralph Burkhardt, Anne Black, Robert Ahrends, Gerhard Liebisch
Summary: This study presents a standardized protocol for quantifying lipid species concentrations in basal and activated human platelets using flow-injection mass spectrometry. The results show changes in lipid species concentrations after platelet activation and the release of complex lipids. These concentrations provide important insights into the physiological role of platelet lipids and serve as a basis for studying the platelet lipidome in patients with hemorrhagic or thrombotic disorders.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Analytical
Dominik Kopczynski, Nils Hoffmann, Nina Troppmair, Cristina Coman, Kim Ekroos, Michael R. Kreutz, Gerhard Liebisch, Dominik Schwudke, Robert Ahrends
Summary: Lipid analysis is important for understanding the various functions of lipids, and LipidSpace is a standalone tool that analyzes lipidomes by assessing their structural and quantitative differences. It offers a user-friendly GUI and support for multiple data formats, allowing for reanalysis and merging of datasets, and provides additional discoveries.
ANALYTICAL CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Ranjithkumar Rajendran, Vinothkumar Rajendran, Gregor Boettiger, Christine Stadelmann, Kian Shirvanchi, Laureen von Au, Sudhanshu Bhushan, Natascha Wallendszus, Darja Schunin, Victor Westbrock, Gerhard Liebisch, Sueleyman Erguen, Srikanth Karnati, Martin Berghoff
Summary: This study demonstrates the therapeutic potential of the FGFR inhibitor infigratinib in preventing and suppressing the first clinical episodes of MOG(35-55)-induced experimental autoimmune encephalomyelitis, reducing inflammation and neurodegeneration. Infigratinib also promotes oligodendrocyte maturation and remyelination, and decreases neurodegeneration-associated lipids. Therefore, infigratinib holds promise as a treatment for multiple sclerosis.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Oncology
Merel van Gogh, Jesus F. Glaus Garzon, Dilara Sahin, Lucia Knopfova, Petr Benes, Onur Boyman, Igor Jurisica, Lubor Borsig
Summary: The authors demonstrate that c-Myb expression in tumor cells induces the expression of immune-related genes, leading to immunomodulation and inhibition of tumor growth.
CANCER IMMUNOLOGY RESEARCH
(2023)
Article
Biology
Sabrina Ehnert, Anna J. Schreiner, Claudine Seeliger, Josef Ecker, Fabian Springer, Gerhard Liebisch, Philipp Hemmann, Tina Histing, Andreas K. Nussler
Summary: Using fatty acid methyl ester gas chromatography-mass spectrometry, this study found that both saturated and unsaturated fatty acids increase in the bone marrow. Characteristic fatty acid patterns were identified in patients with normal BMD, osteopenia, and osteoporosis, and different patterns were found in plasma, red bone marrow, and yellow bone marrow. Specific fatty acids correlated with osteoclast activity and suggested a possible mechanism for how fatty acids interfere with BMD, but no single fatty acid in the profile could be claimed for controlling BMD.