Journal
MOLECULAR NUTRITION & FOOD RESEARCH
Volume 58, Issue 4, Pages 830-841Publisher
WILEY-BLACKWELL
DOI: 10.1002/mnfr.201300112
Keywords
ApoE; mouse; Genistein; Hypercholesterolemia; Lipid peroxidation; Nonalcoholic steatohepatitis
Categories
Funding
- Basic Science Research Program through the National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology [2009-0069120, 2012-014736]
- National Research Foundation of Korea [2009-0069120] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ask authors/readers for more resources
ScopeGenistein (GEN) is a compound that has been shown to alleviate hepatic steatosis. Here, we investigated its protective effects against non-alcoholic steatohepatitis (NASH) development in apolipoprotein E-deficient (ApoE(?/?)) mice fed a high-fat diet (HFD). Methods and resultsWild-type and ApoE(?/?) mice were fed an HFD with or without GEN (0.5 g/kg diet) for 24 weeks. Body weights were reduced and fecal cholesterol excretion was increased by GEN. GEN supplementation lowered serum and hepatic cholesterol and lipid peroxidation levels, and hepatic heme oxygenase 1 protein levels in ApoE(?/?) mice. Hepatic expressions of scavenger receptors involved in oxidized LDL uptake, CD36 and scavenger receptor A, were downregulated by GEN. GEN reduced serum alanine aminotransferase and monocyte chemoattractant protein 1 levels, and hepatic nuclear factor-B-mediated inflammatory gene expressions in ApoE(?/?) mice. These levels were higher in ApoE(?/?) mice fed an HFD than their corresponding wild-type mice. GEN also alleviated hepatic steatosis by reducing mRNA levels of monoacylglycerol O-acyltransferase 1, a target gene of peroxisome proliferator-activated receptor . ConclusionGEN alleviated NASH as well as hypercholesterolemia and obesity in ApoE(?/?) mice fed an HFD. Restoration of altered cholesterol metabolism and oxidative stress may be involved in the protective effect of GEN against NASH development.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available