Journal
MOLECULAR NUTRITION & FOOD RESEARCH
Volume 53, Issue 9, Pages 1123-1133Publisher
WILEY
DOI: 10.1002/mnfr.200800584
Keywords
Aromatic hydroxylation; Catechols; Human liver; Microsomes; Zearalenone
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Funding
- Deutsche Forschungsgemeinschaft [ME 574/32-1]
- KIT
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Zearalenone (ZEN) is a common mycotoxin, for which only reductive metabolites have been identified so far. We now report that ZEN is extensively monohydroxylated by microsomes from human liver in vitro. Two of the major oxidative metabolites arise through aromatic hydroxylation and are catechols. Their chemical structures have been unambiguously determined by using deuterium-labeled ZEN and by comparison with authentic reference compounds. Moreover, both catechol metabolites of ZEN were substrates of the enzyme catechol-O-methyl transferase. One of the monomethyl ethers represented the major metabolite when ZEN was incubated with rat liver slices, thus demonstrating that catechol formation also takes place under in vivo-like conditions. Out of ten major human cytochrome P450 (hCYP) isoforms only hCYPIA2 was able to hydroxylate ZEN to its catechols with high activity. Catechol formation represents a novel pathway in the metabolism of ZEN and may be of toxicological relevance.
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