Article
Neurosciences
Anna Longatti, Luisa Ponzoni, Edoardo Moretto, Giorgia Giansante, Norma Lattuada, Maria Nicol Colombo, Maura Francolini, Mariaelvina Sala, Luca Murru, Maria Passafaro
Summary: Rho GTPases, specifically ARHGAP22, play a critical role in regulating actin dynamics and synaptic function in the CNS. Loss of ARHGAP22 leads to RAC1 hyperactivity and synaptic defects in dendritic spine density, molecular composition, and plasticity, resulting in cognitive impairment and reduced anxiety-like behavior in mice. Inhibiting RAC1 activity restores synaptic plasticity in ARHGAP22 KO mice, highlighting the importance of ARHGAP22 in hippocampal excitatory synapse formation and function, as well as in learning and memory behaviors.
MOLECULAR NEUROBIOLOGY
(2021)
Article
Neurosciences
Courtney K. Walker, Kelsey M. Greathouse, Jennifer J. Tuscher, Eric B. Dammer, Audrey J. Weber, Evan Liu, Kendall A. Curtis, Benjamin D. Boros, Cameron D. Freeman, Jung Vin Seo, Raksha Ramdas, Cheyenne Hurst, Duc M. Duong, Marla Gearing, Charles F. Murchison, Jeremy J. Day, Nicholas T. Seyfried, Jeremy H. Herskowitz
Summary: Proteomic studies on postmortem human brain tissue samples have identified molecular alterations associated with aging and neurodegenerative diseases. To overcome the challenges of identifying individual proteins, researchers developed a blueprint to select and validate targets from proteomic datasets. Through network analysis, they identified TWF2 as a key protein correlated with thin spine length and experimentally validated the finding.
JOURNAL OF NEUROSCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Shu-jian Chen, Cong-cong Gao, Qun-yu Lv, Meng-qi Zhao, Xiao-ying Qin, Hong Liao
Summary: Knocking down sortilin in the prefrontal cortex and hippocampus can alleviate depressive-like behaviors induced by chronic unpredictable mild stress, mainly by decreasing ASM trafficking of ceramide.
ACTA PHARMACOLOGICA SINICA
(2022)
Article
Neurosciences
Bentolhoda Shafiei, Ali Afgar, Mohammad Hadi Nematollahi, Mohammad Shabani, Mahdieh Nazari-Robati
Summary: Aging leads to significant changes in the brain at both the molecular and morphological levels. Impaired antioxidant defense system makes brain cells more vulnerable to oxidative damage. However, the relationship between aging, trehalose, and the expression of miR-132 and SIRT1 in the hippocampus remains poorly understood.
NEUROSCIENCE LETTERS
(2023)
Article
Cell Biology
Tamara Stojanovic, David Velarde Gamez, Gabor Jorrid Schuld, Daniel Bormann, Maureen Cabatic, Pavel Uhrin, Gert Lubec, Francisco J. Monje
Summary: Nicotine addiction mainly develops during adolescence, with miRNA-132/212 playing a role in regulating the effects of nicotine on synaptic functions in the hippocampus of adolescent and adult mice.
Article
Biochemical Research Methods
Liting Deng, Vivek K. Gupta, Yunqi Wu, Kanishka Pushpitha, Nitin Chitranshi, Veer B. Gupta, Matthew J. Fitzhenry, Masoud Zabet Moghaddam, Tim Karl, Ghasem Hosseini Salekdeh, Stuart L. Graham, Paul A. Haynes, Mehdi Mirzaei
Summary: Distinct areas of the brain are affected uniquely at various stages of Alzheimer's disease pathogenesis, with fundamental compositional and functional shifts even in the early stages of the disease. This study highlights unique proteome and biochemical pathway alterations in specific brain regions underlying early stages of AD pathology, providing a framework for future longitudinal studies. The proteomics data have been deposited in the ProteomeXchange Consortium via PRIDE with the identifier PXD019192.
Article
Neurosciences
Xiaofeng Fu, Jing Liu, Junjie Xie, Guanhong Chen, Hao Zhang, Fantao Meng, Min Wu, Qiongyu Li, Yong Liu, Wentao Wang, Juanjuan Dai, Dan Wang, Di Zhao, Chen Li, Xuezhen Wang
Summary: In this study, the researchers investigated the effects of the miR-132-3p/FOXO3a-PPM1F axis on Alzheimer's disease (AD) and found that miR-132-3p indirectly regulates PPM1F expression by targeting FOXO3a. This could be important for early diagnosis, treatment, and understanding the pathogenesis of AD.
Article
Neurosciences
Bo Lei, Jiaxin Liu, Zhihui Yao, Yan Xiao, Xiaoling Zhang, Yueting Zhang, Jianguo Xu
Summary: The study revealed that NF-kappa B-induced upregulation of miR-146a-5p promoted oxidative stress and pyroptosis in AD-HNNs by targeting TIGAR. Knockdown of NF-kappa B or overexpression of TIGAR significantly attenuated oxidative stress and pyroptosis in AD-HHNs, while concurrent overexpression of miR-146a-5p inhibited these effects.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Tamara Stojanovic, Hannah Benes, Amena Awad, Daniel Bormann, Francisco J. Monje
Summary: miRNAs are evolutionarily conserved molecules that regulate gene expression, playing a critical role in cholinergic signaling and neuroplasticity in the nervous system. Deletion of the miR-132/212 gene significantly alters protein levels and synaptic transmission upon nicotine stimulation, highlighting the importance of miRNA/nicotinergic signaling interplay in drug addiction and memory functions.
Article
Neurosciences
Alberto A. Rasia-Filho, Ketlyn T. Knak Guerra, Carlos Escobar Vasquez, Aline Dall'Oglio, Roman Reberger, Claudio R. Jung, Maria Elisa Calcagnotto
Summary: Human pyramidal neurons in the cortical and subcortical areas display diverse morphological features and distribution that significantly enhance the connectivity and complexity of brain circuits. However, these neurons also have vulnerabilities, making them susceptible to neurodegenerative diseases like Alzheimer's and temporal lobe epilepsy.
FRONTIERS IN SYNAPTIC NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Simonetta Pazzaglia, Barbara Tanno, Francesca Antonelli, Paola Giardullo, Gabriele Babini, Prabal Subedi, Omid Azimzadeh, Zohaib N. Khan, Kateryna Oleksenko, Fabian Metzger, Christine von Toerne, Damien Traynor, Dinesh Medipally, Aidan D. Meade, Munira Kadhim, Fiona M. Lyng, Soile Tapio, Anna Saran, Mariateresa Mancuso
Summary: The study demonstrates the potential contribution of non-targeted radiation effects to hippocampal damage, indicating similarities and differences in molecular and histological changes induced by different irradiation doses and methods. It suggests that both targeted and non-targeted radiation effects may play a role in the pathogenesis of hippocampal radiation injury, with implications for human health.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biology
Soile Tapio
Summary: The purpose of this article is to describe the technical development in proteomics over the last two decades, specifically focusing on its application in radiation biology. It is not intended to be a scientific review, but rather a subjective viewpoint on the subject. Proteomics has rapidly evolved and greatly contributed to our understanding of biological mechanisms following radiation exposure, with potential for future use in clinical trials to investigate the effects of ionizing radiation.
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
(2022)
Article
Oncology
Melissa Schwab, Katharina Thunborg, Omid Azimzadeh, Christine von Toerne, Caroline Werner, Maxim Shevtsov, Tommaso Di Genio, Masa Zdralevic, Jacques Pouyssegur, Kathrin Renner, Marina Kreutz, Gabriele Multhoff
Summary: This study shows that inhibiting lactate dehydrogenase can significantly increase the radiosensitivity of tumor cells by impairing the stress response. Therefore, targeting lactate metabolism may serve as a potential strategy to improve clinical outcomes for patients with highly aggressive, therapy-resistant tumors.
Article
Engineering, Biomedical
Sandra Wiedenmann, Markus Breunig, Jessica Merkle, Christine von Toerne, Tihomir Georgiev, Michel Moussus, Lucas Schulte, Thomas Seufferlein, Michael Sterr, Heiko Lickert, Stephanie Ellen Weissinger, Peter Moeller, Stefanie M. Hauck, Meike Hohwieler, Alexander Kleger, Matthias Meier
Summary: This study successfully established the single-cell characterization of the differentiation of human induced pluripotent stem cells into pancreatic duct-like organoids using a microwell chip, and discovered secreted markers of pancreatic carcinogenesis.
NATURE BIOMEDICAL ENGINEERING
(2021)
Article
Public, Environmental & Occupational Health
Omid Azimzadeh, Christine von Toerne, Vikram Subramanian, Wolfgang Sievert, Gabriele Multhoff, Michael J. Atkinson, Soile Tapio
Summary: This study investigated long-term serum proteome alterations following local heart irradiation in mice and identified biomarkers of radiation-induced cardiac toxicity. The analysis showed changes in levels of proinflammatory cytokines and cholesterol metabolism-related proteins in the serum of irradiated mice. These findings provide insights into systemic effects of heart irradiation and may be useful in understanding adverse cardiac effects after radiation therapy.
FRONTIERS IN PUBLIC HEALTH
(2021)
Article
Biochemistry & Molecular Biology
Simonetta Pazzaglia, Barbara Tanno, Ilaria De Stefano, Paola Giardullo, Simona Leonardi, Caterina Merla, Gabriele Babini, Seda Tuncay Cagatay, Ammar Mayah, Munira Kadhim, Fiona M. Lyng, Christine von Toerne, Zohaib N. Khan, Prabal Subedi, Soile Tapio, Anna Saran, Mariateresa Mancuso
Summary: Cell communication via exosomes could influence cell fate under stress situations like ionizing radiation. Studies suggest that exosomes may play a role in radiation effects and resistance to radiotherapy by carrying signaling mediators of radiation damage. There is a lack of comprehensive understanding of exosomes and their regulation under radiation in intact mammalian organisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Timo Smit, Esther Schickel, Omid Azimzadeh, Christine von Toerne, Oliver Rauh, Sylvia Ritter, Marco Durante, Insa S. Schroeder
Summary: This study utilized 3D aggregates of human embryonic stem cell-derived cardiomyocytes to investigate the effects of ionizing radiation and maturity levels of the cells. The matured aggregates displayed increased beat rates and more arrhythmic cellular sequences after X-ray irradiation compared to non-irradiated controls. Proteome analysis provided insight into signaling mechanisms contributing to cardiotoxicity and proposed an in vitro model for screening various noxae targeting adult cardiotoxicity.
Article
Oncology
Barbara Tanno, Flavia Novelli, Simona Leonardi, Caterina Merla, Gabriele Babini, Paola Giardullo, Munira Kadhim, Damien Traynor, Dinesh K. R. Medipally, Aidan D. Meade, Fiona M. Lyng, Soile Tapio, Luca Marchetti, Anna Saran, Simonetta Pazzaglia, Mariateresa Mancuso
Summary: Radiation exposure can lead to heart disease in mice, even when only a portion of the body is irradiated. The study suggests that miRNAs released from irradiated cells may play a role in damaging unirradiated cardiac cells. Understanding the effects of radiation exposure on non-cancer diseases is crucial for developing preventive measures.
Article
Peripheral Vascular Disease
A. Augusto B. Peluso, Stefan J. Kempf, Thiago Verano-Braga, Lucas Rodrigues-Ribeiro, Lene Egedal Johansen, Mie Rytz Hansen, Gitte Kitlen, Andreas Houe Haugaard, Colin Sumners, Henrik J. Ditzel, Robson A. Santos, Michael Bader, Martin R. Larsen, U. Muscha Steckelings
Summary: This study mapped the changes in the phosphorylation status of human aortic endothelial cells in response to AT(2)-receptor stimulation and identified HDAC1 inhibition and p53 activation as novel signaling mechanisms. The study also created a dataset of AT(2)-receptor induced phospho-modified proteins, which can be used for further discoveries.
Article
Biochemistry & Molecular Biology
Birgitte Villadsen, Camilla Thygesen, Manuela Grebing, Stefan J. Kempf, Marie B. Sandberg, Pia Jensen, Stefanie H. Kolstrup, Helle H. Nielsen, Martin R. Larsen, Bente Finsen
Summary: Ceruloplasmin (Cp) is a multicopper oxidase with important roles in iron and copper regulation. Cp deficiency can lead to aceruloplasminemia, which is characterized by neurological symptoms. This study compared the brain proteome in Cp-deficient and wild-type mice, revealing that Cp deficiency affects proteins involved in neuronal projections, synaptic signaling, cellular mRNA processing, and neurodegenerative disease and diabetes. Cp deficiency also affects iron concentration and transferrin saturation, potentially impacting protein and lipid synthesis.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Ilona Barbara Csordas, Eric Andreas Rutten, Tuende Szatmari, Prabal Subedi, Lourdes Cruz-Garcia, David Kis, Balint Jezso, Christine von Toerne, Martina Forgacs, Geza Safrany, Soile Tapio, Christophe Badie, Katalin Lumniczky
Summary: Extracellular vesicles (EVs) play an important role in mediating bystander responses in irradiated bone marrow by altering cellular pathways through the miRNAs they carry. Using a mouse model, we analyzed the miRNA content of BM-derived EVs from mice irradiated with different doses and investigated the proteomic changes in BM cells upon direct irradiation or treatment with EVs. We identified key cellular processes regulated by miRNAs and suggested potential candidates involved in the regulation of these bystander processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Meeting Abstract
Oncology
Melissa Schwab, Katharina Thunborg, Omid Azimzadeh, Christine von Toerne, Maxim Shevtsov, Masa Zdralevic, Jacques Pouyssegur, Kathrin Renner, Marina Kreutz, Peter Vaupel, Gabriele Multhoff
Meeting Abstract
Oncology
Melissa Schwab, Katharina Thunborg, Omid Azimzadeh, Christine von Toerne, Maxim Shevtsov, Masa Zdralevic, Jacques Pouyssegur, Kathrin Renner, Marina Kreutz, Peter Vaupel, Gabriele Multhoff
Article
Biochemical Research Methods
Nina Compera, Scott Atwell, Johannes Wirth, Christine von Toerne, Stefanie M. Hauck, Matthias Meier
Summary: This study developed a microfluidic chip platform to simulate adipose tissue in vitro and revealed the differences in adipocytes under low/high glucose conditions using proteomics analysis. This in vitro model can be used to investigate the molecular and functional mechanisms of adipose tissue under normal and pathological conditions.