Article
Microbiology
Stephany Angelia Tumewu, Hidenori Matsui, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda, Yuki Ichinose
Summary: In this study, four dCache_1 type MCPs in Pta6605 were characterized, with PscA, PscB, and PscC2 responsible for sensing amino acids. Mutant strains of PscA, PscB, and PscC2 showed reduced chemotaxis to most amino acids, as well as effects on cellular functions and virulence in the host tobacco plant.
MICROBIOLOGICAL RESEARCH
(2021)
Review
Plant Sciences
Brian C. Mooney, Melissa Mantz, Emmanuelle Graciet, Pitter F. Huesgen
Summary: Pathogens and hosts are locked in an evolutionary arms race, with pathogen effectors targeting host proteins and host proteins evolving to sense and counteract these effectors. Type III effector proteases are common among bacterial pathogens and modify host proteins irreversibly. Studying effector proteases in plants provides insights into how pathogens evade host immune responses, and how hosts detect and defend against these effectors.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Fisheries
Zhen Tao, Haoda Ye, Chaozheng Zhang, Suming Zhou, Kequan Wang, Mingming Zhang, Jianjun Xie, Hetron Mweemba Munang'andu, Cheng Xu, Pengcheng Wang, Xiaojun Yan
Summary: In this study, a novel two-component system PvgAS has been identified in P. plecoglossicida, which regulates the expression of T6SS-1 and plays a crucial role in bacterial pathogenicity. This finding provides important insights into the understanding of the pathogenic mechanisms of P. plecoglossicida and potential therapeutic interventions against this bacterium.
Article
Microbiology
Ya Jin, Wei Zhang, Shen Cong, Qi-Guo Zhuang, Yi-Lin Gu, Yi-Nan Ma, Melanie J. Filiatrault, Jun-Zhou Li, Hai-Lei Wei
Summary: The bacterial plant pathogen Pseudomonas syringae uses a type III secretion system (T3SS) to deliver effector proteins into plant cells for infection. The T3SS protein HrpP induces host cell death, suppresses pattern-triggered immunity (PTI), and interacts with MKK2 to manipulate plant immunity. This study highlights the importance of HrpP in effector translocation and immune manipulation. Rating: 8/10.
MICROBIOLOGY SPECTRUM
(2023)
Article
Plant Sciences
Chad Fautt, Kevin L. Hockett, Estelle Couradeau
Summary: We evaluated 16 PCR primer sets designed for identifying Pseudomonas syringae species complex using in silico methods. We assessed their amplification rate, correlation between amplicon sequence distance and genome identity, and classification resolution using naive Bayes models. Additionally, we demonstrated the potential of using single amplicon sequence data to predict type III effector protein repertoires, which determine host specificity and range.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Multidisciplinary Sciences
Megan R. O'Malley, Eyram Kpenu, Scott C. Peck, Jeffrey C. Anderson
Summary: Many plant pathogenic bacteria attach to host surfaces during infection, and this attachment is induced by plant signals such as citric acid, glutamic acid, and aspartic acid. These same compounds also induce the expression of genes encoding a type III secretion system (T3SS), suggesting co-regulation of attachment and T3SS deployment. The T3SS master regulator HrpL is partially required for attachment, while the response regulator GacA negatively regulates attachment.
Article
Microbiology
Yezhou Hu, Yanxia Ding, Boying Cai, Xiaohui Qin, Jingni Wu, Minhang Yuan, Shiwei Wan, Yang Zhao, Xiu-Fang Xin
Summary: This study reveals that phytopathogen Pseudomonas syringae induces water soaking in plant leaf tissue by altering the regulation of abscisic acid (ABA), which leads to ABA signaling and stomatal closure. P. syringae achieves this by binding and inhibiting Arabidopsis type one protein phosphatases (TOPPs) that normally suppress ABA signaling. This hijacking of ABA signaling and stomatal closure are key mechanisms of disease susceptibility.
CELL HOST & MICROBE
(2022)
Article
Plant Sciences
Jay Jayaraman, Minsoo Yoon, Lauren M. Hemara, Deborah Bohne, Jibran Tahir, Ronan K. Y. Chen, Cyril Brendolise, Erik H. A. Rikkerink, Matthew D. Templeton
Summary: Testing effector knockout strains of Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) in their native kiwifruit host revealed nonredundant effectors contributing to Psa3 virulence, while complementation in weak kiwifruit pathogen P. syringae pv. actinidifoliorum (Pfm) identified redundant Psa3 effectors. Important Psa3 effectors include those that suppress pattern-triggered immunity (PTI) and early infection process effector redundancy targeting plant immunity hub RIN4.
Review
Biochemistry & Molecular Biology
Jiadai Huang, Chunyan Yao, Yue Sun, Quanjiang Ji, Xin Deng
Summary: This review summarizes the recent advances in the regulatory networks of transcription factors (TFs) involved in virulence-related pathways in Pseudomonas syringae. It also discusses future perspectives in terms of TF-mediated pathogenesis mechanisms and provides novel insights for combating P. syringae infections based on TF regulatory networks.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Plant Sciences
Sachi Kashihara, Takafumi Nishimura, Yoshiteru Noutoshi, Mikihiro Yamamoto, Kazuhiro Toyoda, Yuki Ichinose, Hidenori Matsui
Summary: Pseudomonas amygdali pv. tabaci uses effector proteins to infect tobacco cells, but tobacco has evolved resistance genes and mechanisms. HopAZ1 is identified as a key factor that induces disease resistance in tobacco.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Lu-Lu He, Xin Wang, Dylan O'Neill Rothenberg, Xiaoli Xu, Hai-Hong Wang, Xin Deng, Zi-Ning Cui
Summary: In this study, a high-throughput screening reporter system was constructed to screen for imidazole, oxazole, and thiazole compounds. The screening identified three compounds that significantly inhibited the activity of hrpW and hrpL gene promoters in P. syringae without affecting its growth. These compounds were found to suppress the expression of T3SS genes and inhibit the pathogenicity of P. syringae in tomato and bean. The findings suggest the potential of novel P. syringae T3SS inhibitors for disease prevention and control.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Review
Microbiology
Karl J. Schreiber, Ilea J. Chau-Ly, Jennifer D. Lewis
Summary: Phytopathogenic bacteria use T3SS to deliver T3SEs, which interact with host targets to attenuate immune signaling. T3SEs have diverse biochemical activities and can be difficult to predict without structural data, and some T3SEs are activated following injection into host cells.
Article
Multidisciplinary Sciences
Kinya Nomura, Felipe Andreazza, Jie Cheng, Ke Dong, Pei Zhou, Sheng Yang He
Summary: The AvrE family of bacterial effector proteins functions as membrane channels in plant cells, facilitating pathogen growth and disrupting host cell viability. The elucidation of the biochemical function of AvrE family effector proteins is of great significance in understanding bacterial pathogenesis.
Article
Plant Sciences
Xiaoli Liu, Jun Cai, Xiaoxu Li, Feng Yu, Dousheng Wu
Summary: The article hypothesizes that type III effectors manipulate the microbiota composition by mediating ternary pathogen-plant-microbiota interactions, providing new insights for studying the biological functions of type III effectors and developing new strategies for plant disease control.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Plant Sciences
Saray Santamaria-Hernando, Alvaro Lopez-Maroto, Clara Galvez-Roldan, Marti Munar-Palmer, Elizabet Monteagudo-Cascales, Jose-Juan Rodriguez-Herva, Tino Krell, Emilia Lopez-Solanilla
Summary: This study demonstrates that the perception of GABA and l-Pro by the PsPto-PscC chemoreceptor drives the entry of Pseudomonas syringae pv. tomato into the tomato apoplast. The recognition of these compounds leads to chemoattraction and is involved in the regulation of GABA catabolism. Mutation of the PsPto-PscC chemoreceptor reduces chemotactic response, impairing entry and reducing virulence in tomato plants. Interestingly, GABA and l-Pro levels increase upon pathogen infection and are involved in the regulation of the plant defense response.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Immunology
Emerson Crabill, Whitman B. Schofield, Hayley J. Newton, Andrew L. Goodman, Craig R. Roy
INFECTION AND IMMUNITY
(2018)
Article
Plant Sciences
Emerson Crabill, Anna Joe, Anna Block, Jennifer M. van Rooyen, James R. Alfano
Article
Microbiology
Johana C. Misas-Villamil, Izabella Kolodziejek, Emerson Crabill, Farnusch Kaschani, Sherry Niessen, Takayuki Shindo, Markus Kaiser, James R. Alfano, Renier A. L. van der Hoorn
Article
Microbiology
Hayley J. Newton, Lara J. Kohler, Justin A. McDonough, Morayma Temoche-Diaz, Emerson Crabill, Elizabeth L. Hartland, Craig R. Roy
Article
Plant Sciences
Chia-Fong Wei, Brian H. Kvitko, Rena Shimizu, Emerson Crabill, James R. Alfano, Nai-Chun Lin, Gregory B. Martin, Hsiou-Chen Huang, Alan Collmer