Article
Microbiology
Ashok R. Nayak, Pradip K. Singh, Jinlei Zhao, Montserrat Samso, Michael S. Donnenberg
Summary: Type 4 pili are important surface appendages on bacteria and archaea that can extend and retract with great force. This study presents the detailed structure of an enzyme that converts chemical energy to mechanical energy in a human pathogen, revealing a mechanism of action not previously seen in enzymes that power type 4 pilus extension.
Article
Biochemistry & Molecular Biology
K. Snehaa, Taru Singh, Sajad Ahmad Dar, Shafiul Haque, Vishnampettai G. Ramachandran, Rumpa Saha, Dheeraj Shah, Shukla Das
Summary: This study investigated the prevalence and regional variation of EPEC in paediatric population suffering from diarrhea in East Delhi, India, showing a changing trend of aEPEC over tEPEC in children less than 5 years with diarrhea, emerging as a drug resistant enteropathogen.
BIOMEDICAL JOURNAL
(2021)
Article
Microbiology
Solanka Ellen Ledwaba, David Thomas Bolick, Pedro Henrique Quintela Soares de Medeiros, Glynis Luanne Kolling, Afsatou Ndama Traore, Natasha Potgieter, James Paul Nataro, Richard Littleton Guerrant
Summary: In this study, the role of bundle-forming pili (BFP) in enteropathogenic E. coli (EPEC) infections was evaluated using a mouse infection model. The results showed that disruption of BFP function does not impair pathogenicity, and may even worsen diarrhea, colonization, and intestinal disruption and inflammation. Further research is needed to understand the mechanisms underlying the role of BFP in EPEC infections.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
El-Shama Q. A. Nwoko, Iruka N. Okeke
Summary: Autoaggregation is an important factor in bacterial survival, biofilm formation, and other functions, regulated by various mechanisms. Understanding and studying autoaggregation through qualitative and quantitative methods can lead to the discovery of new therapeutic targets.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2021)
Article
Mycology
Joshua A. Lieberman, Joseph Fiorito, Doug Ichikawa, Ferric C. Fang, Robert M. Rakita, Lori Bourassa
Article
Microbiology
Joshua A. Lieberman, Gregory Pepper, Samia N. Naccache, Meei-Li Huang, Keith R. Jerome, Alexander L. Greninger
JOURNAL OF CLINICAL MICROBIOLOGY
(2020)
Article
Surgery
Joshua A. Lieberman, James A. Mays, Candy Wells, Anne Cent, Deborah Bell, Daniel D. Bankson, Alexander L. Greninger, Keith R. Jerome, Ajit P. Limaye
AMERICAN JOURNAL OF TRANSPLANTATION
(2020)
Article
Immunology
Kristen J. Brao, Brendan P. Wille, Joshua Lieberman, Robert K. Ernst, Mark E. Shirtliff, Janette M. Harro
INFECTION AND IMMUNITY
(2020)
Article
Obstetrics & Gynecology
Michelle C. Starr, Alexander Kula, Joshua Lieberman, Shina Menon, Anthony J. Perkins, Teresa Lam, Shilpi Chabra, Sangeeta Hingorani
JOURNAL OF PERINATOLOGY
(2020)
Article
Pathology
Joshua A. Lieberman, Theresa Nester, Brooke Emrich, Elizabeth M. Staley, Lori A. Bourassa, Hamilton C. Tsang
Summary: The first COVID-19 case in the US was reported in Washington State, leading to significant disruptions in medical institutions and education. The Department of Laboratory Medicine at the University of Washington quickly made substantial changes to medical student clerkships, transitioning to remote learning to continue medical education during the pandemic.
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
(2021)
Article
Microbiology
Sara Rassoulian Barrett, Noah G. Hoffman, Christopher Rosenthal, Andrew Bryan, Desiree A. Marshall, Joshua Lieberman, Alexander L. Greninger, Vikas Peddu, Brad T. Cookson, Stephen J. Salipante
JOURNAL OF CLINICAL MICROBIOLOGY
(2020)
Article
Pediatrics
Joshua A. Lieberman, Caitlin Naureckas Li, Gabriella S. Lamb, David A. Kane, Mary K. Stewart, Ruslan A. Mamedov, Brad T. Cookson, Stephen J. Salipante
Summary: Molecular assays, such as metagenomic next-generation sequencing, are important for infectious disease diagnosis; however, their clinical impact and cost-effectiveness may vary depending on the type of infection and anatomical location. This case study highlights the limitations and challenges of using clinical metagenomics in infectious disease diagnosis.
FRONTIERS IN PEDIATRICS
(2021)
Review
Microbiology
Pradip Kumar Singh, Janay Little, Michael S. Donnenberg
Summary: This article introduces the importance of type IV pili (T4P) and their impact on microbiology, and reviews the important discoveries and research milestones in this field. It also points out some important discoveries that are often overlooked in current reports.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2022)
Article
Microbiology
Ashok R. Nayak, Pradip K. Singh, Jinlei Zhao, Montserrat Samso, Michael S. Donnenberg
Summary: Type 4 pili are important surface appendages on bacteria and archaea that can extend and retract with great force. This study presents the detailed structure of an enzyme that converts chemical energy to mechanical energy in a human pathogen, revealing a mechanism of action not previously seen in enzymes that power type 4 pilus extension.
Article
Microbiology
Andrew P. Platt, Benjamin Bradley, Nadia Nasir, Sydney R. Stein, Sabrina C. Ramelli, Marcos J. Ramos-Benitez, James M. Dickey, Madeleine Purcell, Shreya Singireddy, Nicole Hays, Jocelyn Wu, Katherine Raja, Ryan Curto, Stephen J. Salipante, Claire Chisholm, Stephanie Carnes, Desiree A. Marshall, Brad T. Cookson, Kevin M. Vannella, Ronson J. Madathil, Shahabuddin Soherwardi, Michael T. McCurdy, Kapil K. Saharia, Joseph Rabin, Alison Grazioli, David E. Kleiner, Stephen M. Hewitt, Joshua A. Lieberman, Daniel Chertow
Summary: This study compared the premortem and postmortem detection of pulmonary co-infections in fatal COVID-19 cases. The results showed that the detection of premortem pulmonary co-infections was lower compared to postmortem. Pseudomonas, Enterobacterales, and Staphylococcus aureus were the most frequently identified bacteria, while invasive pulmonary fungal infection was more frequently detected postmortem. Patients with undiagnosed pulmonary co-infections had shorter hospital and ICU stays and fewer extra-pulmonary infections.
Review
Medical Laboratory Technology
Joshua A. Lieberman, Chase A. Cannon, Lori A. Bourassa
Summary: Racial and ethnic disparities in STI in the US are striking and are due to complex interactions of myriad social determinants of health. Budgetary cuts for laboratory and public health services and competition for resources during the COVID-19 pandemic are major challenges. Laboratory professionals must be aware of these underlying issues and work to maximize efforts to ensure equitable access to diagnostic STI testing for all persons, particularly those most disproportionately burdened by STI.
JOURNAL OF APPLIED LABORATORY MEDICINE
(2021)
Article
Medical Laboratory Technology
Joshua A. Lieberman, Lynn G. Stansbury, Joseph Kufera, William C. Chiu, Laurie J. Punch, John R. Hess, Thomas M. Scalea, Sharon M. Henry
JOURNAL OF APPLIED LABORATORY MEDICINE
(2018)
Article
Medicine, Research & Experimental
Joshua A. Lieberman, Kyoko Kurosawa, Dhruba SenGupta, Brad T. Cookson, Stephen J. Salipante, David Busch
Summary: This case report describes a 66-year-old female with culture-negative endocarditis caused by Leptotrichia goodfellowii, identified through reflexive next-generation amplicon sequencing, leading to the patient's death despite additional surgical treatment. The case highlights the importance of advanced molecular diagnostic tools in characterizing serious infections.
COLD SPRING HARBOR MOLECULAR CASE STUDIES
(2021)
Article
Gastroenterology & Hepatology
John J. Garber, Emily M. Mallick, Karen M. Scanlon, Jerrold R. Turner, Michael S. Donnenberg, John M. Leong, Scott B. Snapper
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2018)
