Journal
MOLECULAR MICROBIOLOGY
Volume 79, Issue 1, Pages 264-278Publisher
WILEY
DOI: 10.1111/j.1365-2958.2010.07444.x
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Funding
- National Science Foundation [CBET 0644744]
- Public Health Service [GM083601, GM054365]
- BBSRC [BB/D015855/1]
- Department of Chemical & Biomolecular Engineering at the University of Illinois
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM083601, R01GM054365, R56GM054365] Funding Source: NIH RePORTER
- Biotechnology and Biological Sciences Research Council [BB/D015855/1] Funding Source: researchfish
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The flagellar genes in Salmonella enterica are expressed in a temporal hierarchy that mirrors the assembly process itself. The sigma(28)-FlgM regulatory circuit plays a key role in controlling this temporal hierarchy. This circuit ensures that the class 3 genes are expressed only when the hook-basal body (HBB), a key intermediate in flagellar assembly, is complete. In this work, we investigated the role of the sigma(28)-FlgM regulatory circuit in controlling the timing and magnitude of class 3 gene expression using a combination of mathematical modelling and experimental analysis. Analysis of the model predicted that this circuit continuously controls class 3 gene expression in response to HBB abundance. We experimentally validated these predictions by eliminating different components of the sigma(28)-FlgM regulatory system and also by rewiring the transcriptional hierarchy. Based on these results, we conclude that the sigma(28)-FlgM regulatory circuit continuously senses the HBB assembly process and regulates class 3 gene expression and possibly flagellar numbers in response.
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