Article
Biotechnology & Applied Microbiology
Xuechao Hu, Yangyang Luo, Yanli Man, Xiuyang Tang, Zhiqian Bi, Lujing Ren
Summary: This study investigated the response mechanism of Schizochytrium sp. to temperature stress using transcriptomic and lipidomic approaches. The results showed that high or low temperature inhibited cell growth and affected lipid biosynthesis. Low temperature induced more saturated fatty acids, while high temperature enlarged cell size. Phospholipids were found to be more sensitive to temperature change. The transcriptome analysis revealed the up-regulation of desaturase at low temperature and the up-regulated heat shock proteins at high temperature.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Veterinary Sciences
Luca Palazzese, Federica Turri, Debora Agata Anzalone, Joseph Saragusty, Jacques Bonnet, Marthe Colotte, Sophie Tuffet, Flavia Pizzi, Alessia Luciani, Kazutsugu Matsukawa, Marta Czernik, Pasqualino Loi
Summary: This study adapted a vacuum-drying encapsulation technique to preserve ram spermatozoa, comparing it to traditional lyophilization. The results showed that the encapsulation technique provided better structural stability and higher embryonic development compared to lyophilization. These findings highlight the importance of considering dehydration-related changes in sperm polyunsaturated fatty acids and DNA alterations for successful preservation in large mammals.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Dolores Camacho-Munoz, Magdalena Kiezel-Tsugunova, Orsolya Kiss, Mohib Uddin, Mattias Sunden, Maria Ryaboshapkina, Lars Lind, Jan Oscarsson, Anna Nicolaou
Summary: In this study, the effects of Fibrates and omega-3 polyunsaturated acids on plasma levels of bioactive lipid mediators in patients with obese non-alcoholic fatty liver and hypertriglyceridemia were investigated. The results showed that these two drugs have different effects on the plasma mediator lipidome, with omega-3 promoting lipid mediators potentially affecting chronic inflammation, while Fibrates mainly reducing ceramides. These findings suggest that both treatments could alleviate chronic inflammation and possibly impact disease outcomes, independent of triglyceride reduction.
Article
Agriculture, Multidisciplinary
Ying Shi, Meijing Liu, Yufang Pan, Hanhua Hu, Jin Liu
Summary: This study unraveled the function and role of a Delta 6 fatty acid elongase (No Delta 6-FAE) in EPA biosynthesis in N. oceanica, highlighting the potential of manipulating No Delta 6-FAE for improved lipid production.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Yi Lu, Finnur Freyr Eiriksson, Margret Thorsteinsdottir, Nils Cronberg, Henrik Toft Simonsen
Summary: Bryophytes, such as mosses, liverworts, and hornworts, have attracted researchers due to their diverse chemicals and potential applications in pharmaceutical, food, and cosmetic industries. They accumulate long-chain polyunsaturated fatty acids (l-PUFA) to maintain cell fluidity in cold environments. While Iceland has a rich bryophyte vegetation, the lipid profiles of these plants are largely unknown. In this study, lipidomics analysis was performed on 39 local bryophyte species in Iceland using UPLC-ESI-QTOF-MS, revealing that six moss species had abundant arachidonic acid (AA) and eicosapentaenoic acid (EPA) in their phospholipids and glycerolipids.
Article
Environmental Sciences
Marua Abu Risha, Asghar Ali, Puntita Siengdee, Nares Trakooljul, Dirk Dannenberger, Klaus Wimmers, Siriluck Ponsuksili
Summary: Climate change-induced temperature fluctuations have detrimental effects on the health and productivity of farm animals. This study investigates the impact of temperature stress on muscle cell differentiation, growth, and membrane fatty acid composition, revealing changes in molecular pathways related to lipid metabolism and fatty acid elongation.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Anandita Pal, Shan Sun, Michael Armstrong, Jonathan Manke, Nicole Reisdorph, Victoria R. Adams, Arion Kennedy, Yujiao Zu, Naima Moustaid-Moussa, Ian Carroll, Saame Raza Shaikh
Summary: EPA ethyl esters can improve the metabolic profile of female mice consuming a high fat diet, reducing fat mass accumulation and reversing the effects on circulating insulin, glucose, and inflammatory markers. The mechanisms may involve the biosynthesis of 8-HEPE and the upregulation of intestinal Akkermansia muciniphila, which controls glucose homeostasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2022)
Review
Biochemistry & Molecular Biology
Yingying Zhang, Shuang Wang, Lu Wang, Xiaoyan Chang, Yongxiao Fan, Meiqing He, Dawei Yan
Summary: This review article focuses on the functions of sphingolipids in plasmodesmata, including modulating membrane microdomain organization, architecture transformation, callose deposition and permeability control, as well as signaling regulation. The article also briefly discusses the difference between sphingolipids and sterols, and proposes unresolved questions for better understanding the relationship between plasmodesmal structure and function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Carla Ferreri, Anna Sansone, Chryssostomos Chatgilialoglu, Rosaria Ferreri, Javier Amezaga, Mercedes Caro Burgos, Sara Arranz, Itziar Tueros
Summary: Fatty acids play a crucial role in both biology and nutrition, and their impact on the onset and development of diseases, including cancer, is well known. Using fatty acid-based food and supplements to support cancer therapy requires multidisciplinary research. The composition of membrane fatty acids is essential in membrane lipid therapy. Additionally, fatty acids also serve as biomarkers for cancer and can be personalized for treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Malavika Prithviraj, Takehiro Kado, Jacob A. Mayfield, David C. Young, Annie D. Huang, Daisuke Motooka, Shota Nakamura, M. Sloan Siegrist, D. Branch Moody, Yasu S. Morita
Summary: Tuberculostearic acid, an abundant and genus-specific fatty acid, has been extensively studied as a diagnostic marker for tuberculosis. The discovery of the Cfa gene and its role in the synthesis of tuberculostearic acid provides valuable insights into the mechanisms of mycobacterial membrane compartmentalization. These findings contribute to a better understanding of the survival mechanisms of tuberculosis bacteria.
Article
Biotechnology & Applied Microbiology
Joao P. Monteiro, Elisabete Costa, Tania Melo, Pedro Domingues, Antoine Fort, M. Rosario Domingues, Ronan Sulpice
Summary: This study analyzed the lipid profile of three species of foliose Ulva grown in the same conditions and found important differences in fatty acid composition and sulfolipids. The study suggests that U. australis and U. rigida have higher nutritional value, while U. lacinulata has a lower n-6/n-3 ratio. Additionally, Ulva rigida contains sulfolipid species with potential bioactivity.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Cell Biology
Carla Ferreri, Anna Sansone, Marios G. Krokidis, Annalisa Masi, Barbara Pascucci, Mariarosaria D'Errico, Chryssostomos Chatgilialoglu
Summary: Oxygen plays a crucial role in lipid metabolism, especially in cells with impaired repair mechanisms. This study examined the effects of different oxygen tensions on fatty acid-based membrane remodeling in two cell models. The results showed that oxygen tension influences enzymatic activity and fatty acid composition, suggesting a relationship between oxygen and cellular functions.
Article
Oncology
Anastasia L. Berg, Megan R. Showalter, Nont Kosaisawe, Michelle Hu, Nathanial C. Stephens, Michael Sa, Hailey Heil, Noemi Castro, Jenny J. Chen, Kacey VanderVorst, Madelyn R. Wheeler, Zachary Rabow, Tomas Cajka, John Albeck, Oliver Fiehn, Kermit L. Carraway
Summary: Bis(monoacylglycero)phosphates (BMPs), lipid components within endolysosomal organelles, have been associated with pathological states such as neurodegenerative diseases and lysosomal storage disorders. This study identified lower BMP levels in transformed cells compared to normal cells, and observed an enrichment of 22:6 BMP species in breast cancer models. Functional analysis suggests that 22:6 BMPs serve as reactive oxygen species scavengers, protecting lysosomes from oxidant-induced damage. These findings highlight the potential therapeutic target of BMP-mediated lysosomal protection in breast tumor cells.
Article
Biochemistry & Molecular Biology
Wenke Jonas, Kristin Schwerbel, Lisa Zellner, Markus Jaehnert, Pascal Gottmann, Annette Schuermann
Summary: This study demonstrates that impaired lipophagy contributes to the pathology of NAFLD and is associated with an altered lipid profile. However, the lipid pattern does not appear to be specific for lipophagic alterations, but is more related to fatty liver disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Shaojie Cui, Glenn Simmons, Goncalo Vale, Yaqin Deng, Jungyeon Kim, Hyeonwoo Kim, Ruihui Zhang, Jeffrey G. McDonald, Jin Ye
Summary: This study reveals that Fas-associated factor 1 (FAF1) interacts directly with free polyunsaturated fatty acids (PUFAs) and this interaction is crucial for protecting cells from ferroptosis. FAF1 assembles a globular structure that sequesters free PUFAs into a hydrophobic core, preventing PUFA peroxidation and ultimately inhibiting ferroptosis initiation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Gastroenterology & Hepatology
Yuhan Yin, Anna Sichler, Josef Ecker, Melanie Laschinger, Gerhard Liebisch, Marcus Hoering, Marijana Basic, Andre Bleich, Xue-Jun Zhang, Ludwig Kuebelsbeck, Johannes Plagge, Emely Scherer, Dirk Wohlleber, Jianye Wang, Yang Wang, Marcella Steffani, Pavel Stupakov, Yasmin Gaertner, Fabian Lohoefer, Carolin Mogler, Helmut Friess, Daniel Hartmann, Bernhard Holzmann, Norbert Hueser, Klaus-Peter Janssen
Summary: Hepatocyte growth and proliferation depend on membrane phospholipid biosynthesis, which is significantly influenced by short-chain fatty acids (SCFAs) generated through bacterial fermentation. Antibiotic treatment and dysbiosis not only affect gut microbiota, but also impair hepatic lipid synthesis and liver regeneration.
JOURNAL OF HEPATOLOGY
(2023)
Article
Cell Biology
Vanda Varga-Zsiros, Ede Migh, Annamaria Marton, Zoltan Kota, Csaba Vizler, Laszlo Tiszlavicz, Peter Horvath, Zsolt Torok, Laszlo Vigh, Gabor Balogh, Maria Peter
Summary: Lipidomic technologies have become valuable tools for studying lipid metabolism in various biological fields. However, the evaluation of macroscopic tissue homogenates often overlooks microns-scale heterogeneity. In this study, a laser microdissection-coupled shotgun lipidomic platform was developed to analyze lipid profiles with spatial resolution. The method was validated using mouse liver and kidney samples, as well as a 2D cell culture model. The platform demonstrated good coverage and enabled the recognition of tissue heterogeneity, which could be useful for studying region-specific lipidomic alterations in complex samples, including tumors.
Article
Cardiac & Cardiovascular Systems
Nikolaos Pagonas, Felix S. Seibert, Gerhard Liebisch, Maximillian Seidel, Theodoros Giannakopoulos, Benjamin Sasko, Oliver Ritter, Nina Babel, Timm H. Westhoff
Summary: This study found an association between low levels of propionate, a short-chain fatty acid, and coronary artery disease (CAD).
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2023)
Article
Agriculture, Dairy & Animal Science
Lea Schaefer, Sarah M. Grundmann, Silvia Friedrichs, Dieter Luetjohann, Marcus Hoering, Gerhard Liebisch, Erika Most, Robert Ringseis, Klaus Eder
Summary: Replacing soybean oil with Hermetia illucen larvae fat alters the fatty acid composition of meat and reduces lipid oxidation.
ARCHIVES OF ANIMAL NUTRITION
(2023)
Article
Hematology
Lilli Arndt, Ileana Hernandez-Resendiz, Doreen Moos, Janine Dokas, Silvana Mueller, Franziska Jeromin, Richard Wagner, Uta Ceglarek, Iris M. Heid, Marcus Hoering, Gerhard Liebisch, Sonja C. Stadler, Ralph Burkhardt
Summary: This study demonstrates that deficiency of Trib1 promotes the formation of atherosclerotic lesions and increases plasma cholesterol and triglyceride levels in Ldlr(-/-) mice. It also suggests that Trib1 deficiency alters hepatic lipid metabolism and induces inflammation.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Qingqing Wang, Miriam Hoene, Chunxiu Hu, Louise Fritsche, Robert Ahrends, Gerhard Liebisch, Kim Ekroos, Andreas Fritsche, Andreas L. Birkenfeld, Xinyu Liu, Xinjie Zhao, Qi Li, Benzhe Su, Andreas Peter, Guowang Xu, Rainer Lehmann
Summary: Reliability, robustness, and interlaboratory comparability of quantitative measurements in clinical lipidomics studies are crucial. The different ex vivo stability of lipids in blood poses a risk to data interpretation. Clear recommendations for blood sample collection are necessary. In this study, we used UHPLC-high resolution mass spectrometry to investigate the stability of 417 lipid species in EDTA whole blood under different temperature conditions and time points. Our data suggest that cooling whole blood immediately and continuously, and separating plasma within 4 hours, unless focusing on robust lipids, are recommended. Lists of lipids' ex vivo (in)stability and potential biomarkers in whole blood are provided. Overall, our results contribute to the international efforts in achieving reliable and comparable clinical lipidomics studies in the future.
JOURNAL OF LIPID RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Coenraad Frederik Slabber, Marc Bachofner, Tobias Speicher, Andrii Kuklin, Abbie Elisabeth Fearon, Susagna Padrissa-Altes, Roman Bogorad, Carla Horvath Rudigier, Daria Wust, Sabrina Krautbauer, Marcus Hoering, Gerhard Liebisch, Daniel G. Anderson, Christian Wolfrum, Ulrich Auf Dem Keller, Sabine Werner
Summary: Mice lacking FGF receptors 1 and 2 (Fgfr1 and Fgfr2) in hepatocytes are hypersensitive to cytotoxic injury during liver regeneration, which is associated with the protective role of the urinary-reproductive factor Uhrf2 in liver cells. Uhrf2 expression increases during regeneration after partial hepatectomy in an FGFR-dependent manner, but the abundance of Uhrf2 in liver cells is lower in FGFR-deficient mice. Hepatocyte-specific knockout of Uhrf2 leads to liver necrosis, impaired hepatocyte proliferation, and liver failure.
Article
Multidisciplinary Sciences
Susanne Heimerl, Marcus Hoering, Dominik Kopczynski, Alexander Sigruener, Christina Hart, Ralph Burkhardt, Anne Black, Robert Ahrends, Gerhard Liebisch
Summary: This study presents a standardized protocol for quantifying lipid species concentrations in basal and activated human platelets using flow-injection mass spectrometry. The results show changes in lipid species concentrations after platelet activation and the release of complex lipids. These concentrations provide important insights into the physiological role of platelet lipids and serve as a basis for studying the platelet lipidome in patients with hemorrhagic or thrombotic disorders.
SCIENTIFIC REPORTS
(2023)
Article
Agriculture, Dairy & Animal Science
Lea Schaefer, Sarah M. Grundmann, Garima Maheshwari, Marcus Hoering, Gerhard Liebisch, Erika Most, Klaus Eder, Robert Ringseis
Summary: This study comprehensively investigated the effect of using insect fat from Hermetia illucens larvae as a fat source in broiler diets. The results showed that replacing soybean oil with insect fat had no effect on the growth performance and metabolic health of broilers, suggesting that insect fat can be a sustainable alternative fat source in broiler diets.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2023)
Article
Endocrinology & Metabolism
Fen Long, Memoona R. Bhatti, Alexandra Kellenberger, Wenfei Sun, Salvatore Modica, Marcus Hoering, Gerhard Liebisch, Jean-Philippe Krieger, Christian Wolfrum, Tenagne D. Challa
Summary: This study reveals that a high-fat diet can cause liver fibrosis and non-alcoholic fatty liver disease, and explores the mechanisms through which these diseases occur under strict diet control.
MOLECULAR METABOLISM
(2023)
Article
Chemistry, Analytical
Dominik Kopczynski, Nils Hoffmann, Nina Troppmair, Cristina Coman, Kim Ekroos, Michael R. Kreutz, Gerhard Liebisch, Dominik Schwudke, Robert Ahrends
Summary: Lipid analysis is important for understanding the various functions of lipids, and LipidSpace is a standalone tool that analyzes lipidomes by assessing their structural and quantitative differences. It offers a user-friendly GUI and support for multiple data formats, allowing for reanalysis and merging of datasets, and provides additional discoveries.
ANALYTICAL CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Ranjithkumar Rajendran, Vinothkumar Rajendran, Gregor Boettiger, Christine Stadelmann, Kian Shirvanchi, Laureen von Au, Sudhanshu Bhushan, Natascha Wallendszus, Darja Schunin, Victor Westbrock, Gerhard Liebisch, Sueleyman Erguen, Srikanth Karnati, Martin Berghoff
Summary: This study demonstrates the therapeutic potential of the FGFR inhibitor infigratinib in preventing and suppressing the first clinical episodes of MOG(35-55)-induced experimental autoimmune encephalomyelitis, reducing inflammation and neurodegeneration. Infigratinib also promotes oligodendrocyte maturation and remyelination, and decreases neurodegeneration-associated lipids. Therefore, infigratinib holds promise as a treatment for multiple sclerosis.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Nutrition & Dietetics
Sarah M. Grundmann, Kerstin Ress, Lea Zimmermann, Marcus Hoering, Gerhard Liebisch, Erika Most, Robert Ringseis, Klaus Eder
Summary: A high phosphorus intake is associated with metabolic disorders and this study investigated the impact of a high-phosphorus diet on mouse skeletal muscle lipid composition and gene transcription.
Article
Cell Biology
Thomas Timm, Christiane Hild, Gerhard Liebisch, Markus Rickert, Guenter Lochnit, Juergen Steinmeyer
Summary: The levels of lysophosphatidylcholine (LPC) are elevated in the synovial fluid of patients with osteoarthritis (OA). Different LPC species have varying effects on the regulation of proteins and phospholipids in fibroblast-like synoviocytes (FLSs). IL-1 & beta; has the most significant impact on the regulation of proteins by LPC 16:0, suggesting its potential role in modulating inflammatory and catabolic mechanisms.
Article
Biology
Sabrina Ehnert, Anna J. Schreiner, Claudine Seeliger, Josef Ecker, Fabian Springer, Gerhard Liebisch, Philipp Hemmann, Tina Histing, Andreas K. Nussler
Summary: Using fatty acid methyl ester gas chromatography-mass spectrometry, this study found that both saturated and unsaturated fatty acids increase in the bone marrow. Characteristic fatty acid patterns were identified in patients with normal BMD, osteopenia, and osteoporosis, and different patterns were found in plasma, red bone marrow, and yellow bone marrow. Specific fatty acids correlated with osteoclast activity and suggested a possible mechanism for how fatty acids interfere with BMD, but no single fatty acid in the profile could be claimed for controlling BMD.