Journal
MOLECULAR MEDICINE REPORTS
Volume 18, Issue 4, Pages 3631-3640Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/mmr.2018.9372
Keywords
microRNA-675-3p; esophageal squamous cell cancer; migration; invasion
Categories
Funding
- key development plan for social development of Jiangsu Province [SBE2016750057]
- Jiangsu Provincial Key R & D Program Social Development Clinical Frontier Technology Project (Application of Image-guided Precise Tumor Surgical Equipment in Esophageal Cancer Surgery) [BE2016731]
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Esophageal cancer ranks fourth in cancer-associated mortality in China and the incidence of esophageal adenocarcinoma has risen dramatically over the past two decades. MicroRNA (miRNA/miR) serves a pivotal role in human cancer cell growth, invasion and migration. MiR-675-3p is highly expressed in esophageal squamous cell cancer (ESCC) tissues, and may have an influence on ESCC cell migration and invasion. ESCC tumor tissue samples from 35 patients were profiled. MiR-675-3p expression was confirmed by reverse transcription-quantitative polymerase chain reaction. Manipulation of miR-675-3p via knockdown was carried out with subsequent evaluation of effects on cell proliferation, invasion, migration, and use of western blotting and ELISA assays. MiR-675-3p was overexpressed in ESCC tissues compared with normal tissues, and had higher expression levels in ESCC cells compared with the healthy esophageal epithelial cell line. The results revealed a predominant upregulation of cell migration and invasion ability. MiR-675-3p inhibitor inhibited ESCC cell proliferation, migration and invasion ability. It was also demonstrated that downregulation of miR-675-3p decreased the levels of matrix metalloproteinase (MMP) 2 and 9 and increased the level of E-cadherin. In addition, the effects of miR-675-3p inhibitor on ESCC cell lines were eliminated by con-transfection with miR-675-3p inhibitor and miR-675-3p mimic. In conclusion, the results indicated that miR-675-3p may be involved in the progression of ESCC through regulating ESCC cell migration and invasion capacity via modulating epithelial mesenchymal transition markers (MMP2, MMP 9 and E-cadherin).
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