Journal
MOLECULAR MEDICINE REPORTS
Volume 5, Issue 3, Pages 739-744Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/mmr.2011.700
Keywords
curcumin analogues; ovarian cancer; reactive oxygen species; endoplasmic reticulum stress
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Funding
- National Natural Science Funding of China [30901819]
- Science Foundation of Zhejiang Province of China [Y2090668, Y2110522]
- Zhejiang Medical Science Technology Project [2011KY13049]
- Science and Technology Grant of Wenzhou City [Y20090175]
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The aim of the present study was to investigate the apoptosis of human ovarian cancer cell lines, A2780 and CP70, induced by a novel curcumin analogue, B19. The proliferation of cells was detected with methyl thiazolyl tetrazolium (MTT) assay and apoptosis was examined by flow cytometry. Reactive oxygen species (ROS) were assessed by the fluorescent indicator DCF-DA. The protein expression of the endoplasmic reticulum (ER) stress pathways, GRP78, XBP-1, ATF-4 and CHOP, was examined with Western blotting. A growth inhibitory effect was observed after treatment with B19 in a dose-dependent manner and with more potential than curcumin. At 20 mu M, B19 induced significant apoptosis in CP70 cells. Furthermore, B19 induced the ER stress response, while curcumin had no effect on ER stress. These results suggest that B19 has more effective antitumor properties than curcumin, and is associated with the activation of ER stress and ROS in ovarian cancer cells.
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