Article
Fisheries
Pengfei Chu, Yuchao Zhu, Luqian Xu, Xiangyu Yao, Yan Liang, Xiaojun Zhang
Summary: This study identified and analyzed the functional role of ATG4C, a protein involved in autophagy, in grass carp. ATG4C was found to be widely distributed in grass carp tissues, with high expression in the intestine. It showed a strong response in immune tissues after infection with grass carp reovirus (GCRV), indicating its involvement in the immune response. Additionally, the study confirmed the induction of autophagy and apoptosis by GCRV invasion, and revealed that ATG4C positively regulated autophagy while restricting apoptosis, leading to inhibition of GCRV replication.
Article
Immunology
Changyong Mu, Qiwang Zhong, Yan Meng, Yong Zhou, Nan Jiang, Wenzhi Liu, Yiqun Li, Mingyang Xue, Lingbing Zeng, Vikram N. Vakharia, Yuding Fan
Summary: In this study, a recombinant baculovirus expressing VP35-VP4 proteins from GCRV was generated and used to vaccinate grass carp through oral administration. The results showed that the vaccine induced both non-specific and specific immune responses, providing protection against GCRV infection with a relative percent survival rate of 56%. This study suggests that the silkworm pupae powder could be used as an effective oral vaccine for grass carp.
Article
Virology
Wentao Zhu, Meihua Qiao, Meidi Hu, Xingchen Huo, Yongan Zhang, Jianguo Su
Summary: This study found that Type II grass carp reovirus (GCRV-II) rapidly invades grass carp through the nostril, gill, and skin on the head, rather than merely by adhesion. Further investigations revealed that GCRV-II invades grass carp via the nostril-olfactory system-brain axis, and viral replication occurs.
Article
Fisheries
Baohong Xu, Hang Su, Changjun Chen, Qiaolin Liu, Tiaoyi Xiao, Yi Liu, Zhao Lv, Hongquan Wang, Zheyu Wen
Summary: The interaction of C5a and C5aR in grass carp infected with grass carp reovirus promotes the activation of p38 and ERK proteins, leading to increased expression of IL-6 and TNF-alpha and induction of an inflammatory response. These findings provide a theoretical basis for regulating the interaction of C5a and C5aR to alleviate grass carp hemorrhagic diseases.
AQUACULTURE REPORTS
(2023)
Article
Virology
Ling Yang, Jianguo Su
Summary: In this study, it was found that GCRV can only infect leukocytes in vitro, while erythrocytes and thrombocytes are not susceptible. Additionally, the expression levels of immune response-related genes were significantly up-regulated in infected leukocytes.
Article
Virology
Rui Jiang, Jie Zhang, Zhiwei Liao, Wentao Zhu, Hang Su, Yongan Zhang, Jianguo Su
Summary: This study investigated the latent infection of GCRV-II in asymptomatic grass carp with GCRV infection or exposure history. It was found that GCRV-II was detectable only in the brain of grass carp during latent infection, unlike the multi-tissue distribution observed in natural infection. Additionally, GCRV-II distribution in grass carp was notably affected by ambient temperature. This study provides insights into the mechanisms of GCRV-II latent infection and reactivation, contributing to the prevention and control of GCRV pandemics.
Article
Fisheries
Xiaoqing Xiong, Hong Yang, Chunhua Ding, Beibei Qin, Yadong Deng, Liming Xiong, Xiaoyan Liu, Yaoguo Li, Tiaoyi Xiao, Zhao Lv
Summary: In this study, a newly discovered integrin αvβ3 in grass carp was found to interact with viral proteins and mediate antiviral immune response. Furthermore, the expression of this integrin was significantly upregulated in various tissues of grass carp after infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Immunology
Qingqing Tian, Xingchen Huo, Qian Liu, Chunrong Yang, Yongan Zhang, Jianguo Su
Summary: This study developed a prophylactic grass carp reovirus (GCRV) vaccine using virus-like particle (VLP) assembly technology, and the highly immunogenic candidates in the vaccine were VP4, VP56, and VP35. Expression of VP4, VP56, and VP35 in both Escherichia coli and Pichia pastoris systems resulted in successful assembly of uniform, stable, and non-toxic VP4/VP56/VP35 VLPs. Vaccination with P. pastoris VLPs significantly improved survival rate, IgM levels, tissue viral load, and antioxidant immune-related indicators in grass carp.
Article
Immunology
Chuang Xu, Meihua Qiao, Xingchen Huo, Zhiwei Liao, Jianguo Su
Summary: The study screened antigenic epitopes of GCRV-II and prepared an oral microencapsulated vaccine using SA as a carrier and FlaB as an adjuvant. The vaccine showed strong immune responses in grass carp, significantly increasing survival rates and potentially serving as an oral vaccine against GCRV-II infection in aquaculture.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
Hongquan Wang, Hong Yang, Rui Li, Yaoguo Li, Tiaoyi Xiao
Summary: By comparing the expression variation of 13 immune factors in different tissues, it was found that the high gene expression in the coagulation complement and cytokine interaction pathways may be the main reason for the strong resistance of barbel chub to GCRV. However, high gene expression was not a factor affecting GCRV resistance between grass carp populations.
AQUACULTURE INTERNATIONAL
(2021)
Article
Immunology
Nan Wang, Jiahao Li, Yajun Wang, Yingying Wang, Defeng Zhang, Cunbin Shi, Yingying Li, Sven M. Bergmann, Xubing Mo, Jiyuan Yin, Qing Wang
Summary: In this study, Lactococcus lactis was used as an oral vaccine delivery system to express the VP6 protein of grass carp reovirus II (GCRV II). It was found that the recombinant L. lactis strain could induce mucosal immune protection, enhance nonspecific immune responses, and elicit both systemic and mucosal immunity in fish. This study suggests that L. lactis is a promising candidate for oral vaccine delivery in the aquaculture industry.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
De-Kui Qiu, Yan Xiong, Yu-Ming Gong, Yu-Ying Zheng, Rui Ma, Chen Zhang, Gao-Xue Wang, Bin Zhu
Summary: The study found that grass carp vaccinated with pcDNA3.1-vp4 showed better immune responses compared to pcDNA3.1-vp4-3, with the latter having a higher survival rate in preventing GCRV infection.
Article
Fisheries
Yexuan Zhang, Xudong Hu, Shuai Liu, Man Zhou, Chunling Wang, Hong Cao
Summary: This study used next-generation sequencing (NGS) technology to analyze the expression of long noncoding RNAs (lncRNAs) in grass carp kidney cells infected with grass carp reovirus (GCRV). The results showed that 37 lncRNAs and 1039 mRNA transcripts exhibited differential expression after GCRV infection compared to mock infection. Functional analysis indicated that the differentially expressed lncRNAs were mainly involved in biological regulation and cellular processes, such as MAPK signaling pathway and Notch signaling. Furthermore, the upregulation of lncRNA3076 (ON693852) after GCRV infection was observed, and silencing lncRNA3076 decreased GCRV replication, suggesting its important role in GCRV replication.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biology
Beibei Qin, Tiaoyi Xiao, Chunhua Ding, Yadong Deng, Zhao Lv, Jianming Su
Summary: This study identified 42 CiTRIMs in grass carp, with some TRIMs-B30.2 genes associated with the type I interferon response during GCRV infection, providing potential antiviral immune molecule markers for grass carp.
Article
Biochemistry & Molecular Biology
Xinyu Liang, Yongming Li, Pengfei Chu, Qian Wang, Hanyue Wang, Lanjie Liao, Cheng Yang, Zuoyan Zhu, Yaping Wang, Libo He
Summary: This study cloned and analyzed the peroxiredoxin 3 gene from grass carp and found that it can enhance host antioxidant activity and induce autophagy to inhibit the replication of grass carp reovirus.
Article
Fisheries
Huabing Wang, Feifei Zheng, Aotian Ouyang, Gailing Yuan, Jianguo Su, Xiaoling Liu
Summary: This study cloned and functionally characterized MaCSF-1 and MaCSF-1R from blunt snout bream, and found that they play crucial roles in macrophage proliferation, phagocytosis, and IL-10 production.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Zhensheng Wang, Chuang Xu, Yanqi Zhang, Xingchen Huo, Jianguo Su
Summary: The study confirms that the use of nanoparticles CMCS-20a can efficiently prevent grass carp reovirus (GCRV) infection, enhance the immunity of fish, and alleviate tissue damage caused by the virus.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Meihua Qiao, Liqiang Zhang, Chuang Xu, Xingchen Huo, Jiao Chang, Jianguo Su
Summary: The study found that chitosan and anisodamine, as adjuvants for immersion immunization, can enhance the resistance and survival rate of black spotted frogs to Elizabethkingia miricola. This vaccination method is simple and can prevent infectious diseases in the breeding industry of black spotted frogs.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Jiao Chang, Wentao Zhu, Xingchen Huo, Meihua Qiao, Chunrong Yang, Yongan Zhang, Jianguo Su
Summary: In this study, a recombinant Lactobacillus casei expressing multiple fragments of the key outer capsid protein of grass carp reovirus (GCRV) was constructed, and an oral microcapsule live vaccine (SCL-56310-500) was prepared. The results showed that this vaccine effectively enhanced the immune response and suppressed GCRV replication in fish, leading to a significantly higher survival rate.
Article
Fisheries
Yanqi Zhang, Jianguo Su
Summary: This review discusses the unique biological effects of IL-2 family cytokines in regulating differentiation, survival, and activation of multiple lymphocyte lineages, as well as their expression profiles, receptors, signaling transductions, and functions in teleosts. The IL-2 family cytokines in teleosts show relatively conserved functions in lymphocyte proliferation, differentiation, development, and inflammation, similar to their counterparts in mammals.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Immunology
Wentao Zhu, Yanqi Zhang, Zhiwei Liao, Xingchen Huo, Chunrong Yang, Yongan Zhang, Jianguo Su
Summary: This study reveals the strong antibacterial activity of type I interferon IFN1 in teleosts, which is mediated by phagocytosis and induction of immune factors and complement components. These findings shed light on the functional role of interferons in bacterial infections.
JOURNAL OF IMMUNOLOGY
(2023)
Article
Fisheries
Weixiang Zhang, Zhenjun Zhao, Jiancheng Zhou, Weicheng Wang, Jianguo Su, Gailing Yuan
Summary: This study evaluates the feasibility and biological activity of carboxymethyl chitosan (CMCS) loaded with Ctenopharyngodon idella interferon-gamma 2 (CiIFN-gamma 2), and examines the resistance to Aeromonas hydrophila infection in grass carp. The results show that CMCS-CiIFN-gamma 2 nanoparticles can enhance the expression of pro-inflammatory cytokines and the effective uptake of the protein, leading to improved immune protection against bacterial diseases in aquaculture.
Article
Fisheries
Qian Liu, Xingchen Huo, Qingqing Tian, Pengxu Wang, Fengxia Zhao, Chunrong Yang, Jianguo Su
Summary: The oral P-MCP-FlaC vaccine effectively controls LMBV infection in largemouth bass, improving the survival rate and up-regulating protective enzyme activity and immune gene expression.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Aotian Ouyang, Mengwei Zhang, Gailing Yuan, Xiaoling Liu, Jianguo Su
Summary: This study reveals that chitooligosaccharide (COS) has the ability to reverse cortisol-induced immunosuppression in fish and enhance the immune activity of macrophages. Oral administration of COS directly absorbed through the intestine significantly improves the innate immunity of blunt snout bream, leading to increased survival and reduced tissue damage. Thus, COS offers potential strategies for immunosuppression prevention and control in fish.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Virology
Wentao Zhu, Meihua Qiao, Meidi Hu, Xingchen Huo, Yongan Zhang, Jianguo Su
Summary: This study found that Type II grass carp reovirus (GCRV-II) rapidly invades grass carp through the nostril, gill, and skin on the head, rather than merely by adhesion. Further investigations revealed that GCRV-II invades grass carp via the nostril-olfactory system-brain axis, and viral replication occurs.
Article
Immunology
Qingqing Tian, Xingchen Huo, Qian Liu, Chunrong Yang, Yongan Zhang, Jianguo Su
Summary: This study developed a prophylactic grass carp reovirus (GCRV) vaccine using virus-like particle (VLP) assembly technology, and the highly immunogenic candidates in the vaccine were VP4, VP56, and VP35. Expression of VP4, VP56, and VP35 in both Escherichia coli and Pichia pastoris systems resulted in successful assembly of uniform, stable, and non-toxic VP4/VP56/VP35 VLPs. Vaccination with P. pastoris VLPs significantly improved survival rate, IgM levels, tissue viral load, and antioxidant immune-related indicators in grass carp.
Article
Multidisciplinary Sciences
Rui Jiang, Wentao Zhu, Zhiwei Liao, Chunrong Yang, Jianguo Su
Summary: TLR7 plays a crucial role in sensing viral ssRNA and dsRNA, but there are differences in the mechanism of dsRNA sensing between fish and tetrapods.
Article
Virology
Rui Jiang, Jie Zhang, Zhiwei Liao, Wentao Zhu, Hang Su, Yongan Zhang, Jianguo Su
Summary: This study investigated the latent infection of GCRV-II in asymptomatic grass carp with GCRV infection or exposure history. It was found that GCRV-II was detectable only in the brain of grass carp during latent infection, unlike the multi-tissue distribution observed in natural infection. Additionally, GCRV-II distribution in grass carp was notably affected by ambient temperature. This study provides insights into the mechanisms of GCRV-II latent infection and reactivation, contributing to the prevention and control of GCRV pandemics.
Article
Fisheries
Pengxu Wang, Xingchen Huo, Fengxia Zhao, Qian Liu, Qingqing Tian, Chunrong Yang, Jianguo Su
Summary: This study aimed to investigate the potential effectiveness of VD3 in treating Largemouth bass ranavirus (LMBV). The results showed that feeding Largemouth bass with 15000 IU/Kg and 20000 IU/Kg of VD3 significantly suppressed and cleared LMBV pathogens. In addition, VD3 treatment increased the expression levels of key immune-related genes and enhanced antioxidant and immune activities in the serum. Tissue pathology sections also revealed a dose-dependent relationship between VD3 supplementation and tissue damage.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Maolin Lv, Jingjing Zhang, Weicheng Wang, Rui Jiang, Jianguo Su
Summary: This study corrected the number of grass carp TLR20 members, identified a new TLR20 member, and analyzed its evolutionary and structural characteristics. The results showed that TLR20 plays an important role in antiviral and antibacterial defense.
FISH AND SHELLFISH IMMUNOLOGY REPORTS
(2023)
