Article
Virology
Henrike P. Ahsendorf, Ulrike S. Diesterbeck, Sven-Kevin Hotop, Michael Winkler, Mark Broenstrup, Claus-Peter Czerny
Summary: A human single-chain fragment variable (scFv) library was constructed and an anti-F13 scFv was isolated and characterized. The antigenic binding sites of the scFv were determined. The study suggests that constructing recombinant immunoglobulin libraries is a promising strategy for studying host-pathogen interactions.
Article
Radiology, Nuclear Medicine & Medical Imaging
Philipp Diebolder, Cedric Mpoy, Jalen Scott, Truc T. Huynh, Ryan Fields, Dirk Spitzer, Nilantha Bandara, Buck E. Rogers
Summary: Glycoprotein CD44 and its splice variants are overexpressed in many cancers and cancer stem cells, with hyaluronic acid binding to CD44 to activate cell signaling pathways. Developing a CD44-specific antibody fragment for imaging CD44-positive cancers using PET shows potential clinical utility in cancer therapy.
JOURNAL OF NUCLEAR MEDICINE
(2021)
Article
Veterinary Sciences
Aodi Wu, Yueli Wang, Adnan Ali, Zhenyu Xu, Dongsheng Zhang, Kairat Zhumanov, Jinliang Sheng, Jihai Yi
Summary: This study utilized immunoinformatics approach and advanced computer software to identify and analyze important antigenic parts of Brucella abortus, leading to the design of a chimeric peptide vaccine that can effectively stimulate the immune system's response to the bacteria.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Immunology
Alistair Noble, Basudev Paudyal, John C. Schwartz, William Mwangi, Danish Munir, Elma Tchilian, John A. Hammond, Simon P. Graham
Summary: Cattle possess three IgG subclasses with different functionalities, which differ from those described in humans, mice, or pigs. This has important implications for the development of vaccines and therapeutic antibodies.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Wissam Charab, Matthew G. Rosenberger, Haridha Shivram, Justin M. Mirazee, Moses Donkor, Soumya R. Shekhar, Donjeta Gjuka, Kimberly H. Khoo, Jin Eyun Kim, Vishwanath R. Iyer, George Georgiou
Summary: Elevated levels of circulating immune complexes are associated with autoimmunity and worse prognoses in cancer. IgG-ICs inhibit the proliferation and differentiation of a subset of naive T cells while stimulating the division of another naive-like T cell subset. Phenotypic analysis revealed immature features in the inhibited T cell subset and transcriptional features indicative of a more differentiated phenotype in the stimulated T cell subset.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Kai-Thomas Schneider, Toni Kirmann, Esther Veronika Wenzel, Jan-Hendrik Grosch, Saskia Polten, Doris Meier, Marlies Becker, Paul Matejtschuk, Michael Hust, Giulio Russo, Stefan Duebel
Summary: Generation of sequence defined antibodies from universal libraries by phage display has been established as a robust method. ScFv-Fcs can be effective as neutralizing antibodies against a panel of pathogens and toxins, but may have variable stability. A generic lyophilization protocol has been developed to improve their shelf life, showing preservation of initial properties without compromising binding activity after storage.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Ulrike S. Diesterbeck, Henrike P. Ahsendorf, Andre Frenzel, Ahmad Reza Sharifi, Thomas Schirrmann, Claus-Peter Czerny
Summary: A panel of potent neutralizing antibodies offers protection against orthopoxvirus infections. In an in vivo experiment, purified scFv-1.2.2.H9 and IgG1-1.2.2.H9 partially protected against the challenge with VACV, while only one mouse survived in the scFv-Fc-1.2.2.H9 group.
Article
Biotechnology & Applied Microbiology
Yaqi Yang, Siji Nian, Lin Li, Xue Wen, Qin Liu, Bo Zhang, Yu Lan, Qing Yuan, Yingchun Ye
Summary: Enhanced EphA2 expression is a crucial target for anti-tumor therapy, with high affinity scFvs against EphA2 successfully screened from an immune library constructed using PBMCs from cancer patients. Modified antibodies showed improved affinity and the ability to bind to EphA2, tumor cells, and tissues, inhibiting tumor growth to some extent. This study highlights the potential of immune libraries from cancer patients in screening for high-affinity antibodies with therapeutic effects.
Article
Biotechnology & Applied Microbiology
Lei Guo, Xiuze Chen, Haifeng Zeng, Na Tian, Weijie Lu, Jizhou Zhang, Yechen Xiao
Summary: This study presents a novel approach for expressing IL-18BP fusion protein in E. coli by fusing it with SUMO and IgG1 Fc fragments. The resulting protein showed high yield and soluble expression, and demonstrated anti-inflammatory effects in cell lines and a mouse model.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Review
Pharmacology & Pharmacy
Hajarossadat Ghaderi, Atefeh Alipour, Arya Mohammadi Zadeh Holagh, Fatemeh Kazemi Lomedasht, Meghdad Abdollahpour-Alitappeh, Hooman Kaghazian, Hosein Shahsavarani
Summary: Antibodies have attracted significant attention as a treatment for various diseases, but their size, side effects, and cost have limited their effectiveness. As a result, researchers have developed recombinant antibodies with small size, high resistance, and rapid clearance to overcome these limitations.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Sara M. A. Mohamed, Andreas Wohlmann, Peter Schofield, Keith C. S. Sia, Hannah McCalmont, Savvas N. Savvides, Kenneth Verstraete, Maria Kavallaris, Daniel Christ, Karl-Heinz Friedrich, Narges Bayat, Richard B. Lock
Summary: Antibody fragments show promise in targeted therapeutics by reducing leukemia cell survival through specific protein targeting. This study introduces a novel scFv fragment targeting CRLF2, which exhibits excellent binding affinity and potential therapeutic implications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Immunology
Wenfeng Xu, Lei Peng, Chun Li, Tong Wu, Han Chen, Haiyan Zhang, Hong Yu, Yingchun Ye, Yuchuan Wu, Qing Yuan, Siji Nian
Summary: This study successfully developed a fully human antibody that neutralizes the alpha-hemolysin toxin of Staphylococcus aureus, providing a new theoretical basis and treatment method for preventing MRSA infection.
Review
Virology
Noemie Oechslin, Maliki Ankavay, Darius Moradpour, Jerome Gouttenoire
Summary: Hepatitis E virus (HEV) is now considered as one of the most frequent causes of acute hepatitis worldwide, but it has received relatively little attention for decades. Recent advances in the molecular virology of HEV have allowed study of the entire viral life cycle and exploration of host factors required for infection.
Article
Biochemical Research Methods
Ilnaz Soleimani Mashhadi, Mohammad Reza Safarnejad, Morteza Shahmirzaie, Atousa Aliahmadi, Alireza Ghassempour, Hassan Y. Aboul-Enein
Summary: The study proposes an approach to improve the accuracy of recognition of epitopic peptide interaction rate through investigating the interaction between the nucleocapsid protein of fig mosaic virus (FMV-NP) and single-chain variable fragment antibodies (scFv-Ab). This method combines scFv-Ab with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and involves using two different enzymes and various incubation times, offering a precise and dependable analysis for monitoring and recognizing the binding kinetics of antigenic determinants.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Medicine, Research & Experimental
Roland Tschismarov, Raphael M. Zellweger, Min Jie Koh, Yan Shan Leong, Jenny G. Low, Eng Eong Ooi, Christian W. Mandl, Katrin Ramsauer, Ruklanthi de Alwis
Summary: The study found that after immunization with the MV-CHIK vaccine, participants displayed strong antibody responses, with the prime-boost regimen showing greater and longer-lasting protection compared to the prime-only regimen.
Review
Biotechnology & Applied Microbiology
Nina E. Weisser, J. Christopher Hall
BIOTECHNOLOGY ADVANCES
(2009)
Article
Biochemistry & Molecular Biology
Margaret A. Johnson, Jonathan Cartmell, Nina E. Weisser, Robert J. Woods, David R. Bundle
JOURNAL OF BIOLOGICAL CHEMISTRY
(2012)
Article
Immunology
Nina E. Weisser, Kurt C. Almquist, J. Christopher Hall
Article
Medicine, Research & Experimental
Mario Sanches, Igor D'Angelo, Maria Jaramillo, Jason Baardsnes, John Zwaagstra, Joe Schrag, Ian Schoenhofen, Mauro Acchione, Sam Lawn, Grant Wickman, Nina Weisser, David K. Y. Poon, Gordon Ng, Surjit Dixit
