Journal
MOLECULAR IMMUNOLOGY
Volume 46, Issue 5, Pages 793-802Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.molimm.2008.09.002
Keywords
Fc epsilon RI; Endosomes; Co-localization; Syk
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Funding
- Division of Intrainural Research
- NIAID/NIH
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In addition to initiating signaling cascades leading to mast cell mediator release, aggregation of the high affinity IgE receptor (Fc epsilon RI) leads to rapid internalization of the cross-linked receptor. However, little is known about the trafficking of the internalized Fc epsilon RI. Here we demonstrate that in RBL-2H3 cells, aggregated Fc epsilon RI appears in the early endosomal antigen 1 (EEA1(+)) domains of the early endosomes within 15 min after ligation, Minimal co-localization of Fc epsilon RI with Rab5 was observed by 30 min, followed by its appearance in the Rab7(+) late endosomes and lysosomes at later time points. During endosomal sorting, Fc epsilon RI alpha and gamma Subunits remain associated. In Syk-deficient RBL-2H3 cells, the rate of transport to lysosomes is markedly increased. Taken together, our data demonstrate time-dependent sorting of aggregated Fc epsilon RI within the endosomal-lysosomal network, and that Syk may play an essential role in regulating the trafficking and retention of Fc epsilon RI in endosomes. Published by Elsevier Ltd.
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